Method For Determination Of Taurine Research Articles

Determination of Taurine in Quadriceps Femoris by High Performance Liquid Chromatography with Precolumn Derivatization

A method for determination of taurine in mouse quadriceps femoris by high performance liquid chromatography with precolumn derivatization ophthalaldehyde (OPA) was established. The sample was extracted and treated with canon exchange resin. Results showed that taurine in quadriceps femoris was separated and quantified on C18 reversed phase column by high performance liquid chromatographic (HPLC) after derivatization with OPA in 3min, using mixture of methanol and phosphate （V/V＝1:1，pH＝4.9） as mobile phase, rate of flow is 0.6mL/min, detecting at 340nm by UV-detector, L-Glutamine as internal standard. The result showed that the linear ranger of taurine was 6.25-187.7ng/mL, correlation coefficient R2=0.9994, the recoveries were 91.8%-101.8%, RSD=3.2% (n=6). The retention time of taurine is 7.32 min. The concentration of taurine in mouse quadriceps femoris is 3.18mg/g. The protein and amino acid were separated by sample pre-treatment. The method is of good separation effect, simple, reliable, and can be used to analyze the taurine concentration in mammal tissue.

A Development of An Analytical Spectrophotometeric Method for determination of Taurine in Energy Drinks

A Development of An Analytical Spectrophotometeric Method for determination of Taurine in Energy Drinks

Spectrophotometric Method for Determination of Taurine in Energy Drinks

Spectrophotometric Method for Determination of Taurine in Energy Drinks

UV-Spectrophotometry Determination of Taurine in Energy Drink Mixtures

The aim of this study is developing and validation of UV-spectrophotometric method for determination of taurine in energy drink mixtures. The investigation includes validation of procedures for performance of the tests for identification, purity and assay. Analytical parameters precision, accuracy, selectivity, linearity, limit of detection and limit of quantitation were studied and compared. Developed method allows selectively determination of taurine in the present of caffeine at different conditions (algorithm, wavelength zones). The preferences are estimation the analytical parameters from the validation procedures. They let to compose the criteria in accordance with European Pharmacopoeia and EU regulates about the use of official analytical programs for quality control of supplements containing taurine. The method has been applied successfully in analysis of energy food drinks preparation in the appointed for each of their aspect of applications.

Quantitative on-chip determination of taurine in energy and sports drinks

A new method for the quantitative determination of taurine in beverages by microchip electrophoresis was developed. A rapid and simple sample preparation procedure, only including two dilution steps and the addition of the fluorogenic labeling reagent NBD-Cl (4-chloro-7-nitrobenzofurazan), is applied. Using a home-built wavelength-resolved fluorescence detector, the separation and determination of the taurine derivative could be achieved in only 12 s, while the additional spectral information was utilized to ensure peak purity. Spanning from 0.1 to 50 mmol L(-1), the linear dynamic range of the applied method was adapted to the apparent contents in common taurine containing beverages. The smallest detectable amount of the taurine derivative actually injected into the separation channel was as low as 60 amol. The method was successfully validated by an independent liquid chromatographic method.

Taurine Analysis in Milk and Infant Formulae by Liquid Chromatography: Collaborative Study

A collaborative study was conducted on a liquid chromatographic (LC) method for determination of taurine in infant formula and milk powders. Twenty laboratories participated in the analysis of 8 blind duplicates over the range of approximately 3-60 mg/100 g sample. The method involved protein removal, conversion to the dansyl-derivative, and isocratic LC separation with UV and/or fluorescence detection. Following outlier treatment, overall mean RSDR has been estimated at 7.00% for supplemented products with a HORRAT value of 1.1. The poorer precision at endogenous levels establishes a lower limit of determination of about 5 mg/100 g. An overall mean RSDr:RSDR value of 0.7 for all products demonstrated acceptable performance.

Development of An HPLC-UV Method for Determination of Taurine in Infant Formulae and Breast Milk

A rapid and accurate high performance liquid chromatographic (HPLC) procedure was proposed for routine and selective determination of taurine in infant formulas and breast milk. The sample preparation was simple, involving protein removal and filtration operations. Afterwards, taurine was derivatised with o-phtha]aldehyde/2-mercaptoethanol prior to injection onto a reversed phase column C18 (S10ODS2). Isocratic elution was carried out using 0.05 M phosphate buffer pH 5.3/ methanol (60:40) mixture. The effluent was monitored by a UV detector at 350 nm. A linear relationship was found between peak area and a concentration range of 1–70 μg/mL. The detection limit was 0.3 μg/mL. Effective separation and quantification was achieved in under six minutes. No interference of other amino acids was observed. Extensive validation of the proposed method was carried out both by the standard additions method and by comparison with a fluorimetric method of known accuracy. The precision was better than 1.4%.

Determination of Taurine in Infant Formulas Using Ultrafiltration and Cation-Exchange Chromatography

A fast and simple method for determination of taurine in infant formulas has been developed. The sample preparation uses disposable ultrafiltration cartridges to remove protein and clarify the sample. Hydrolysis is avoided, simplifying the procedure and increasing efficiency. One mL sample is centrifuged in a cartridge for 45 min. The filtrate is diluted with pH 2.2 citrate buffer and injected into a high performance amino acid analyzer. A cation-exchange column (sodium phase) is used with a single buffer eluant and an isocratic chromatographic program. Colorimetric detection is performed following post-column ninhydrin reaction. Chromatographic resolution from other ninhydrin-positive compounds is excellent. Average recoveries for 3 levels of spike for various products were 100-102%. Precision is 1-3% RSD, depending on product. Linearity, specificity, and ruggedness are excellent. The method is applicable to quality control testing of milk-based, soy-based, and prehydrolyzed protein-based infant formulas in the ready-to-use, concentrate, and powder forms. A variety of commercially available infant formulas from different manufacturers were analyzed and all were found to contain taurine levels comparable to human milk. Some human milk and cow's milk samples were also analyzed and results compare well with literature values.