The local stress concentration created by the seam welding process often reduces the fatigue life of the material along and around the seam. Post-welding heat treatment is usually used to improve the weld performance, but the improvement effect on fatigue life may not be obvious. In this study, a new treatment method of electromagnetic coupling was applied to regulate the Ti2AlNb electron beam weld after heat treatment, so as to improve the fatigue properties of the welded joint. The results show that after electromagnetic coupling treatment, the fatigue limit of the welded joint is increased by 10.4 %, the residual compressive stress is increased, and the fatigue crack propagation rate is decreased. The microstructure analysis shows that after electromagnetic coupling treatment, the fatigue crack source starts in the subsurface layer, the substructural crystals in the fusion zone and the heat affected zone decrease, the recrystallization content and the large angle grain boundary increase, and the dislocation density increases and tends to be homogenized. An increase of at least 2.3 % in hardness of the welded joints also indicates the increase in the dislocation density. Local high density dislocation is found in the grain, which causes dislocation entanglement and hinders crack initiation and propagation and this provides an important basis for improving fatigue properties. This study provides a new method for improving the fatigue properties of Ti2AlNb electron beam welds which can also be used to study the properties of other welded joints.