Methanol Extract Of Fruits Research Articles

Adult food and species type influence the nutritional physiology and tolerance of two flour beetles to the extract of Dennettia tripetala (G. Baker)

AbstractTribolium castaneum (Herbst) and Tribolium confusum (Jacquelin du Val) are common and damaging pests of stored grain flours in several homes and flour mill industries worldwide. This study examines how food, species, concentration, and exposure time affect the susceptibility and nutritional physiology of T. castaneum and T. confusum when exposed to partially purified methanolic fruit extract of Dennettia tripetala (G. Baker) and two of its major active compounds (linalool and 2‐phenylnitroethane). Gas chromatography–mass spectrometry was used to evaluate the chemical profile of the partially purified plant extract. Thirty‐two compounds were found in the plant, including established insecticidal and insectifuge compounds such as linalool, cis‐vaccenic acid, 2‐phenylnitroethane, and linoleic acid, among others. The interactions among the factors have a differential impact on the susceptibility and nutritional indices of both Tribolium species to D. tripetala, linalool and 2‐phenylnitroethane. According to LC50 values, linalool and 2‐phenylnitroethane were the most and least toxic to both beetles, respectively. With increasing extract concentrations, all nutritional indices except feeding deterrence decreased. Relative to controls, linalool and 2‐phenylnitroethane evoked the highest and least reduction, respectively, in beetles' nutritional indices. Treated corn discs deterred both species over treated wheat discs, regardless of insecticide type. T. castaneum had a higher overall food consumption than T. confusum. Regardless of insecticide type, the relative growth rate was higher in T. confusum and T. castaneum fed with treated corn and wheat discs, respectively. D. tripetala extract, linalool, and 2‐phenylnitroethane showed higher post‐ingestion toxicity to T. castaneum than T. confusum. This study emphasises the importance of certain intrinsic factors, like beetles' species and cereal food types, when managing Tribolium species with plant‐based insecticides. The various data obtained from this study will help researchers and pesticide manufacturers formulate effective biopesticides from linalool and 2‐phenylnitroethane to control Tribolium species on corn and wheat flours.

Exploring the phytochemical, antioxidant, antimicrobial and analgesic potentials of Solanum erianthum as an alternative biological feedstock for producing sustainable biochemicals

Solanum erianthum is a medicinal herb that offers health benefits and valuable phytochemicals in treating human ailments. Phytochemicals and therapeutic properties of different parts of S. erianthum in four solvents: water, petroleum ether, chloroform, and methanol were evaluated. Stem showed high alkaloids (15.40 ± 0.7%) and tannins (8.6 ± 0.3%), followed by higher root saponins (12.2 ± 0.2%). In contrast, leaf exhibited high flavonoid (13.5 ± 0.5%) and phenolic (12.30 ± 1.0%) contents. The petroleum ether extract of the stem had the highest 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity (70.66 ± 0.3%) and ferric reducing antioxidant power (FRAP) activity (70.55 ± 0.08 μg/mL). In contrast, the petroleum ether extract of the root had maximum total antioxidant capacity (TAC) (1.065 ± 0.6 absorbance at 695 nm), while the fruit aqueous extract showed highest activity in ferric thiocyanate assay (FTC) (61.53 ± 0.1). Antibacterial activity ranged from 19 to 51 mm, while antifungal was from 11 to 43 mm. The methanol extracts of the root and leaf showed the highest antimicrobial activity against the tested bacteria and fungi. The methanol extract of fruit showed the highest analgesic activity (11.98 ± 2.14 s) and writhing inhibition (1st phase 85.1% and 2nd phase 96.7%), as confirmed by the formalin-induced paw licking test. Fruit extract showed active central and peripheral analgesic effects in early (88.9%) and late (92.8%) phases of pain, comparable to paracetamol (60 mg/kg). In conclusion, this study demonstrates the antioxidant, antimicrobial, and antifungal properties as well as central and peripheral analgesic effects of S. erianthum extracts.

Quality control methods for fruit extracts of Kigelia africana using high performance thin layer chromatography

Kigelia africana is a tree native to Africa but also found in eastern and southern parts of India with reported anti-bacterial, anti-inflammatory, and immunomodulatory activities. Verbascoside, caffeic acid and ferulic acid are important markers for the quality control of the plant. Two different HPTLC methods were developed and validated; method − 1 for estimation of verbascoside and caffeic acid while method − 2 for estimation of caffeic acid and ferulic acid. Developed methods were applied to the methanolic fruit extract to determine the quantities of markers. Both methods were found to be linear, specific, precise, accurate, sensitive and robust. Results indicated that both methods can be used for quantitative determination of verbascoside, caffeic acid and ferulic acid in fruit extract. The developed methods may be utilised as a part of the quality control and standardisation for the raw material and extracts of Kigelia africana and can also aid to chromatographic fingerprinting of the plant.

Ethno‐Botanical Study of Medicinal Plants from Vidisha, Madhya Pradesh: Methanolic Extracts of Four Species and their Antimicrobial, Anti‐Inflammatory and Toxicity Properties

AbstractThe traditional usage of Plumbago zeylanica L, Cassia alata L, Caesalpinia crista L, and Rubia Cordifolia L in the villages of Vidisha district in Madhya Pradesh, India, has sparked research into the plants' phytochemical makeup and a range of possible medicinal benefits. This research is the first to describe the phytochemical makeup, powerful anti‐inflammatory, and antioxidant properties of PZ stem. The PZ stem and CA fruit methanolic extracts has the greatest levels of phenolic, flavonoid, and flavonol compounds and has a robust antioxidant capacity for scavenging DPPH and hydrogen peroxide with regard to A. flavus, A. baumannii, A. fumigatus, S. aureus, K. pneumonia, and R. oryzae, the anti‐bacterial and anti‐fungal activity of the chosen plant extracts are strongest for PZ, CA, and CA T. terrestris leaf.

Anticancer, Antioxidant, Antimicrobial Activities, and HPLC Analysis of Alcoholic Extracts of Parthenocissus quinquefolia L. Plant Collected from Çanakkale

This study aimed to contribute to revealing the health effects of the Parthenocissus quinquefolia L. (PQ) plant by examining the biological activities of various extracts of the plant. An ethnobotanical important medicinal plant, PQ belongs to the Vine family (Vitaceae) and is known for its medicinal uses. Herein, fruit and leaf alcoholic extracts of the plant exhibited anticancer activity on triple negative breast cancer (TNBC) cell lines, estrogen receptor positive (ER+) (MCF-7), and human colon adenocarcinoma (HT-29) cancer cell lines. Furthermore, fruit methanol extracts displayed strong radical scavenging activity with low IC50 values such as 0.51 mg/mL, 0.80 mg/mL, 0.84 mg/mL, and 1.45 mg/mL. The highest TEAC values (18.72 mM Trolox/mg, 9.90 mM Trolox/mg, and 8.41 mM Trolox/mg) were calculated for the extracts of red leaves. The antifungal activity was determined against Candida albicans as fungal pathogen. The cell apoptosis with methanol extract was obtained between 20 µg/mL and 70 µg/mL for 48 h and 72 h treatments by Hoechst 33258 staining of nuclei under a fluorescence microscopy. HPLC analysis was performed for methanol extracts to determine major bioactive components. Consequently, this plant extracts may guide to new strategies and may be uses in medicinal applications for further investigations.

Antibacterial Profiling of Zanthoxylum armatum Extracts: A Comprehensive Computational and Experimental Study

Objectives The current study was conducted to evaluate the antibacterial potential of leaf and fruit extracts of Zanthoxylum armatum against two pathogenic bacterial isolates, Staphylococcus aureus and Staphylococcus epidermidis. Methods Twelve commercially available antibiotics were tested S. aureus and S. epidermidis by antimicrobial susceptibility test (AST). Qualitative analysis of phytochemicals was performed to evaluate the presence of certain secondary metabolites. The activity of Z. armatum extracts against S. aureus and S. epidermidis was measured as a maximum zone of inhibition exhibited by each leaf and fruit extract. An in-silico study was conducted on flavonoids and alkaloids to show their binding affinity with the PBP2a receptor protein of S. aureus and TcaR of S. epidermidis. Results The AST revealed that S. aureus was resistant to Penicillin, Ampicillin, Clindamycin, Vancomycin, Rifampicin, Novobiocin, and Oxacillin, whereas S. epidermidis was resistant to Streptomycin, Oxacillin, Tetracycline, and Novobiocin. Qualitative analysis of phytochemicals resulted in the presence of Saponins, fixed oils, flavonoids, alkaloids, starch, and fatty acids in both leaf and fruit extracts. The maximum zone of inhibition against S. aureus was produced by methanolic leaf extracts of Z. armatum and chloroform fruit extracts. For S. epidermidis, the best activity was exhibited by benzene leaf extracts and methanolic fruit extracts. An in-silico study showed that flavonoids Nitidine and Nevadensin exhibited binding affinity with the PBP2a receptor protein higher than selected antibiotics, ie, Penicillin, Chloramphenicol, and Oxacillin. TcaR of S. epidermidis interacted with Tambuletin, followed by Nitidine and Kaempferol. Conclusion After in vitro testing, in silico analysis advised extracting and purifying the bioactive components from Z. armatum extracts that showed significant interaction with bacterial virulence proteins for use as natural antibiotics against antibiotic-resistant bacteria.

Protective effect of Phaleria macrocarpa Methanolic Fruit Extract against Oxidative Stress in Brine Shrimps

Phaleria macrocarpais one of the commonly usedplants in East Asia as herbal medicines. The extract of Phaleria macrocarpa can exhibit a variety of beneficial pharmacological activities, including anti-diabetic, and antioxidant activities which are primarily contributed by the constituents isolated from different parts of Phaleria macrocarpa. However, there have been only limited attempts to explore the antioxidant properties of this plant in relation to its medicinal use. Therefore, this study was conducted to determine the antioxidant activity and protective effect of P. macrocarpa methanolic fruit (PMFM) extract against oxidative stress in brine shrimp (Artemia salina). The antioxidant activity of PMFM extractwas evaluated using 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays.In vitro brine shrimp toxicity assay was performed on nauplii of Artemia salina to determine the toxicity and the protective effects of the PMFM extract. The DPPH and FRAP assays results showed that PMFM extracthas good antioxidant activity.Additionally, treatment with PMFM extract (concentration ranging from 0.05 to 0.2 mg/ml) was able to protect Artemia salinanauplii from the toxic effects of hydrogen peroxide.Thus, the present findings demonstrate that PMFM extractpossesses good antioxidant and protective activity against oxidative stress.

Antidiabetic activity of methanolic extract of Hibiscus sabdariffa Linn. fruit in alloxan-induced Swiss albino diabetic mice

Abstract The majority of natural diabetic medications come from fruits and vegetables. These natural medications help protect humans from negative impacts of chemical antidiabetics by scavenging free radicals. The present study aimed to explore the antioxidant and antidiabetic properties of methanolic extract of fruits of Hibiscus sabdariffa Linn. (MEHSF) in alloxan-induced Swiss albino diabetic mice. The dried coarse powder of Hibiscus sabdariffa Linn. fruits was subjected to methanol extraction. The antidiabetic activity was determined by using alloxan-induced (80 mg/kg body weight) diabetic mice. Following a 15-day treatment period, serum biochemical parameters including total cholesterol (TC), triglyceride (TAG), LDL-cholesterol (LDL), HDL-cholesterol (HDL), serum glutamic pyruvic transaminase (SGPT), and serum glutamic-oxaloacetic transaminase (SGOT) enzymes were estimated. The antioxidant activity was evaluated through a DPPH and ABTS free radical scavenging assay. Total phenolic content and total flavonoid content were assessed using established methods. MESHF, containing polyphenolic and flavonoid compounds, exhibited antioxidant properties. A 100 and 200 mg/kg significantly (p < 0.05) lowered the blood glucose levels and improved biochemical parameters such as TC, TAG, LDL, and HDL in diabetic mice. Further, MESHF significantly (p < 0.05) reduced the activity of the SGPT and SGOT in diabetic mice compared to untreated diabetic mice. These results suggest that MEHSF with promising antioxidant and antidiabetic potentials can be considered to be a probable new resource of the antidiabetic agent.

Hepatoprotective effect of methanol fruit extract of Punica granatum L in highly active antiretroviral therapy-induced toxicity in Wistar rats

Prolonged use of Highly Active Antiretroviral Therapy (HAART) has been linked to toxicity, particularly hepatotoxicity. There are few effective drugs for HAART patients that promote hepatic cell regeneration and prevent liver injury. Therefore, the purpose of this study was to investigate the hepato-protective activity of Methanol fruit extract of Punica granatum (MFEPG) in HAART-administered rats. Thirty rats weighing between 150–200 g were randomly divided into six groups and each group comprised of five rats. Distilled water was given to the rats in group one. Only HAART was given to the rats in group two. MFEPG at doses of 100 and 400 mg/kg was given to the rats in groups three and four. MFEPG dosages of 100 and 400 mg/kg along with HAART were given to the rats in groups five and six, respectively. All treatments were via oral gavage daily for 40 days. Under halothane anesthesia, all rats were sacrificed on day 41. Liver tissues were utilized for lipid peroxidation marker; Malondialdehyde (MDA), antioxidant enzymes; Superoxide dismutase (SOD) and Catalase (CAT) and histological evaluation, while blood samples were examined for biochemical parameters (AST, ALT, ALP, Total cholesterol, Total protein, and Albumin). The HAART-treated group exhibited a significantly higher amount of the lipid peroxidation end product; MDA, and significantly lower levels of antioxidant enzymes; SOD, and CAT. Liver enzymes and total cholesterol were significantly increased with a significant reduction in Total protein and Albumin levels in the HAART-treated group. Conversely, the liver function biomarkers were returned to normal levels in the HAART and MFEPG-treated groups. Histopathological studies revealed that when HAART-exposed rats were treated with MFEPG, both the biochemical and histological results significantly improved. Thus, the antioxidant activity of MFEPG provides protection against HAART-induced liver oxidative damage. More research is needed to determine the safety of using MFEPG in humans.

Nephroprotective effect of the methanolic fruit extract of Musa balbisiana Colla against carbon tetrachloride-induced toxicity in Swiss albino mice

Nephroprotective effect of the methanolic fruit extract of Musa balbisiana Colla against carbon tetrachloride-induced toxicity in Swiss albino mice

PHYTOCHEMICAL AND ANTICONVULSANT SCREENING OF THREE MEDICINAL PLANTS USED IN THE TREATMENT OF EPILEPSY IN THE SOUTH-WESTERN PART OF NIGERIA

The burden of epilepsy in developing countries made medicinal plants like Xylopia aethiopica fruit; Khaya grandifoliola, Alstonia boonei etc an alternative source in epilepsy management in the south-western part of Nigeria. The aim of the study was to provide pharmacological rationale for the ethnomedicinal use of the plants in epilepsy management. The oral medial lethal dose of methanol stem bark extracts of Alstonia boonei (MEAB) and Khaya grandifoliola (MEKG) and methanol fruit extract of Xylopia aethiopica (MEXAF) were done in accordance with the Organization for Economic Cooperation Development guideline. Quantitative and qualitative phytochemical profiling of the extracts was done. Anticonvulsant screening was carried out on the extracts (doses: 75, 150 and 300 mg/kg) using the pentylenetetrazole (PTZ)-induced seizure and maximum electroshock tests (MEST). Results showed that the MEXAF has the highest amount of phytochemicals except for saponins in MEKG; and MEAB with the least amount (but higher alkaloid) than MEKG. The TLC showed different bands of spots of the extracts. In the PTZ test, MEXAF showed 100 % protection against mortality at 300 mg/kg; MEAB with 66.67 % protection at 75 mg/kg and MEKG 0 % protection. MEAB, MEKG and MEXAF nonsignificantly increased the onset of seizure and latency to death. In the MEST, MEXAF, MEKG and MEAB at 75 mg/kg protected 50, 33.3 and 16.67% of the animals against tonic hind limb extension respectively and nonsignificantly (p˃0.05) decreased recovery time except MEXAF which significantly (p<0.05) decreased the recovery time at a dose of 75 mg/kg. It was concluded that the extracts possess anticonvulsant activities hence, the pharmacological credence for the ethnomedicinal use of these plants in treating epilepsy.

Anti-oxidative constituents of Musa balbisiana Colla fruit extract and evaluation of hepatoprotective activity in CCl4-induced hepatotoxicity in Swiss albino mice

Carbon tetrachloride is employed to induce hepatotoxicity in experimental animals to mimic oxidative stress caused by several factors in humans and impair liver functions and lead to hepatic damage. Fruits of Musa balbisiana Colla are rich in bioactive compounds and hold therapeutic and nutritional worth. This current study aims to explore the phytochemical constituents, antioxidant effects, and hepatoprotective activity of a methanolic extract derived from the unripe fruit pulp of M. balbisiana Colla via phytochemical screening, evaluation of antioxidant activity, in vivo experiments in Swiss albino mice through monitoring of the body and the liver weight, estimation of hepatic biomarkers, enzymes, malondialdehyde (MDA), and cytokines along with immunohistochemical and histopathological study. The methanolic fruit extract administration minimized hepatic biomarker and MDA levels along with the cytokines and also elevated superoxide dismutase, catalase, plus glutathione content compared to the hepatotoxic group (p < 0.05). Immunohistochemical findings and histopathological examinations of the liver tissues from methanolic extract-treated mice displayed the down regulation of transforming growth factor beta in hepatic parenchymal tissues and re-establishment of standard structural attributes of hepatic tissue. The outcomes of our research illustrate that the methanolic fruit extract of M. balbisiana Colla administration assists in hepatotoxicity attenuation.

Unveiling the functional components and anti-Alzheimer's activity of Koelreuteria elegans (Seem.) A.C. Sm. using UHPLC-MS/MS and molecular networking

The metabolomic profiles of Koelreuteria elegans leaf and fruit methanol extracts using UHPLC-MS/MS analysis aided by molecular networking were explored. Both extracts reduced all the examined markers of inflammation and neurodegeneration in the injured streptozotocin (STZ)-induced AD mice.

Acute anti-inflammatory effect of methanolic extracts of leaf, bark and fruit of Averrhoa bilimbi on carrageenan-induced acute inflammation in rats

Acute anti-inflammatory effect of methanolic extracts of leaf, bark and fruit of Averrhoa bilimbi on carrageenan-induced acute inflammation in rats

Total phenolic content and antioxidant activities in methanol extracts of medicinal herbs from Indo-Gangetic plains of India

The present study investigates total phenolics, flavonoids, and in vitro antioxidant activities in methanol extracts of roots, stems, leaves, and fruits of medicinal herbs (Phyllanthus fraternus G.L. Webster; Bhui-amla and Solanum nigrum L.; Makoi or black nightshade) of Indo-Gangetic plains of India. The results showed that biochemical attributes of methanol extracts of both the tested plants varied significantly with sites (P < 0.05). Contents of total phenolics and flavonoids and antioxidant properties were found highest in fruits followed by leaf, stem, and least in root of P. fraternus and leaves, fruits, stem, and roots of S. nigrum plants. The present investigation revealed that total phenolic content in fruits of P. fraternus ranged from 26.69 to 61.48 mg GAE/g fw and from 8.89 to 24.69 mg GAE/g fw in leaves of S. nigrum plants, and thus, these plant parts can be promoted for pharmaceutical purposes and health benefits. It is also suggested that the individual phenolic compound in the different parts of tested plants should be analyzed to identify their elite population for their mass cultivation, conservation, and sustainable utilization.

Isolation of quinic acid from dropped Citrus reticulata Blanco fruits: its derivatization, antibacterial potential, docking studies, and ADMET profiling.

Citrus reticulata dropped fruits are generally discarded as waste, causing environmental pollution and losses to farmers. In the present study, column chromatography has been used to isolate quinic acid (1,3,4,5-tetrahydroxycyclohexane-1-carboxylic acid) from the ethyl acetate fraction of a methanol extract of citrus fruits dropped in April. Quinic acid is a ubiquitous plant metabolite found in various plants and microorganisms. It is an important precursor in the biosynthesis of aromatic natural compounds. It was further derivatized into 3,4-o-isopropylidenequinic acid 1,5-lactone (QA1), 1,3,4,5-tetraacetoxycyclohexylaceticanhydride (QA2), and cyclohexane-1,2,3,5-tetraone (QA3). These compounds were further tested for their antibacterial potential against the foodborne pathogens Staphylococcus aureus, Bacillus spp., Yersinia enterocolitica, and Escherichia coli. QA1 exhibited maximum antibacterial potential (minimum inhibitory concentration; 80-120μg/mL). QA1 revealed synergistic behavior with streptomycin against all the tested bacterial strains having a fractional inhibitory concentration index ranging from 0.29 to 0.37. It also caused a significant increase in cell constituent release in all the tested bacteria compared to the control, along with prominent biofilm reduction. The results obtained were further checked with computational studies that revealed the best docking score of QA1 (-6.30kcal/mol, -5.8kcal/mol, and -4.70kcal/mol) against β-lactamase, DNA gyrase, and transpeptidase, respectively. The absorption, distribution, metabolism, excretion, and toxicity (ADMET) analysis revealed that the drug-like properties of QA1 had an ideal toxicity profile, making it a suitable candidate for the development of antimicrobial drugs.

The Potential Role of Phytochemicals of Juniperus procera in the Treatment of Ovarian Cancer and the Inhibition of Human Topoisomerase II Alpha Activity.

A variety of active chemicals found in medicinal plants can be used to develop new medications with few adverse effects. In vitro and in silico analyses were used to evaluate the anticancer properties of Juniperus procera fruit and leaf extracts. Here, we show that the methanolic extract from J procera fruit and leaf extracts inhibits 2 human ovarian cancer cell lines, A2780CP and SKOV-3. The leaf extract demonstrated strong cytotoxicity against A2780CP with an IC50 of 1.2 μg/mL, almost matching the IC50 of the anticancer medication doxorubicin (0.9 μg/mL). Higher antioxidant activity was observed in the fruit than leaf extract. The molecular docking results showed that the active component, podocarpusflavone A, was the best-docked chemical with the human topoisomerase II alpha enzyme. According to our knowledge, this is the first in vitro study to show the cytotoxicity of J procera extracts against the 2 previously described human ovarian cancer cell lines. The fact that the podocarpusflavone A molecule may have an inhibitory effect on the human topoisomerase II alpha enzyme was also revealed by this first in silico analysis. Our findings imply that the J procera fruit and leaf methanolic extract has anticancer characteristics that may guide future in vivo studies.

Phytochemical, Antioxidant and Anti-Diabetic Activity Scrutinization in the Methanolic Fruit Extract of Syzygium jambos

Phytochemical, Antioxidant and Anti-Diabetic Activity Scrutinization in the Methanolic Fruit Extract of Syzygium jambos

Pengaruh Ekstrak Metanol Buah Lakum (Cayratia trifolia L. Domin) terhadap Nilai SGOT dan SGPT Tikus Putih Diinduksi Parasetamol

Lakum fruit (Cayratia trifolia L. (Domin)) has secondary metabolite compounds that are beneficial to health, one of which is a hepatoprotector. This study was conducted to prove the potential of hepatoprotective activity of lakum fruit methanol extract based on the value of SGPT and SGOT enzyme. The research method used a pre-posttest randomized control group design with a sample of 35 male rats divided into five treatment groups: solvent control group (CMC 0.5%); positive control group (HEPA-Q®); treatment group extract (0,575; 1,15; 1,725 mg/g BW). The treatment of induction of paracetamol and methanol extract of Cayratia trifolia fruit was carried out for 7 days respectively. The results showed that the dose of 1,15 mg/g BW of Cayratia trifolia fruit methanol extract had the most effective deterioration of SGOT and SGPT values with a percentage reduction of SGOT of 49.3% and SGPT of 51.8% (p <0.05) which approached control positive (HEPA-Q®). Based on the research conducted, methanol extract of Cayratia trifolia fruit has the potential to be hepatoprotective.

Assessment of Phenolic Composition, Antioxidant Potential, Antimicrobial Properties, and Antidiabetic Activity in Extracts Obtained from Schinus molle L. Leaves and Fruits.

The current research centers on exploring the antioxidant, antimicrobial, and antidiabetic features of Schinus molle L. grown in Turkey. Quantitative analysis of chlorogenic acid, caffeic acid, and hyperoside levels in leaf, ripe fruit, and raw fruit extracts was conducted using High-Performance Liquid Chromatography (HPLC) in a 70% methanol-water mixture. Among the extracts, the methanol extract from ripe fruits displayed the highest chlorogenic acid concentration, measuring at 2.040% ± 0.172% standard deviation (SD). Moreover, analysis of their total phenolic and flavonoid contents was carried out. Antioxidant power was assessed through different chemical assays, together with their antimicrobial and anti-diabetic properties. The results of DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and reducing power assays showed that leaf and ripe fruit alcoholic extract exhibited peak performance. While the MIC ( minimum inhibitory concentration) values of the extracts were determined to have moderate bactericidal effects on Staphylococcus aureus, Escherichia coli, and Candida albicans it was observed that none of the extracts displayed biofilm inhibition. The inhibition percentage of α-glucosidase enzyme activity for the methanol extract of raw fruits was determined to be 99.11 ± 1.61. In diabetic β-TC cells, glucose level was measured as 129 ± 2.03 mg/dL, and insulin amount was measured as 37.2 ± 0.02 mg/dL. The findings of our study seem to have important implications for future research, as Schinus molle L. may be a potential pharmaceutical candidate with important pharmacological activities.