Composition Of Methanol Research Articles

Prediction of methanol loss by hydrocarbon gas phase in hydrate inhibition unit by back propagation neural networks

Gas hydrate often occurs in natural gas pipelines and process equipment at high pressure and low temperature. Methanol as a hydrate inhibitor injects to the potential hydrate systems and then recovers from the gas phase and re-injects to the system. Since methanol loss imposes an extra cost on the gas processing plants, designing a process for its reduction is necessary. In this study, an accurate back propagation neural network (BPNN) is designed for the prediction of methanol loss by the gas phase as a function of temperature, pressure, and methanol composition in the aqueous phase. Different configurations of BPNN were trained, tested, and a configuration providing the smallest absolute average relative deviation (AARD%) was chosen as an optimum structure. Finally, comparisons made among the accuracy of the developed BPNN model, process simulators, and probabilistic neural network (PNN). Results confirm that the designed BPNN model is more accurate than the other considered predictive tools. The BPNN provided an AARD=5.75% for prediction of experimental data, while Aspen-HYSYS, Aspen-Plus, and PNN presented an AARD% of 9.71, 12.57, and 13.27, respectively.

STABLE LIQUID CHROMATOGRAPHY METHOD FOR THE RESOLUTION AND QUANTIFICATION OF RELATED IMPURITIES OF FOSAMPRENAVIR IN BULK AND PHARMACEUTICAL FORMULATIONS

Highly resolved and validated Liquid Chromatography method was developed for the separation and quantification of fosamprenavir and its related impurity 2 and 5 in bulk and pharmaceutical formulations. Separation of fosamprenavir and its impurities was achieved on prontosil ODS C18 column using mobile phase composition of methanol and 0.1 m sodium acetate in the ratio of 40:60 (v/v) at pH 5.9 as mobile phase at a flow rate of 0.9 mL/min in isocratic condition. Uv detection of the eluents was monitored at a wavelength of 246nm. In these conditions, well resolved peaks were observed at a retention time of 8.67, 5.73 and 4.00 min for fosamprenavir, Impurity 2 and 5, respectively. Calibration curve was plotted in the concentration range of 75-450 µg/mL for fosamprenavir and 1-6 µg/mL for impurity 2 and 5. Forced degradation study confirms that the method can separate the known and unknown impurities of fosamprenavir and the % degradation was found to be very less in all the stress conditions. Hence the method is suitable for the identification and quantification of impurities 2 and 5 along with fosamprenavir in bulk drug and formulations.

One-step photolytic synthesis of hydrophobic porous polymer materials by the copolymerization of the dimethacrylate—alkyl methacrylate system in the presence of methanol

Hydrophobic porous polymer materials were synthesized by one-step visible light polymerization from compositions consisting of phthalate-containing oligoester di(meth)acrylate MDP-2, methanol, and alkyl methacrylate (butyl methacrylate, ethylhexyl methacrylate, iso-decyl methacrylate, lauryl methacrylate, and stearyl methacrylate) compositions. The highest hydrophobization effect is observed for stearyl methacrylate. The maximum MDP-2 block photopolymerization rate is by 16–50 times higher than that of alkyl methacrylates. The specific surface areas of the synthesized porous polymers reach 4.2 m2 g−1, and the pore size ranges from 5.5 to 17 µm. The addition of stearyl methacrylate to the composition leads to the pore size distribution narrowing. The principal possibility of using these photopolymerizable compositions as stationary phases for liquid chromatography is shown.

The study of solid-liquid equilibrium for dimethyl terephthalate (DMT) in several solvents from T = (298.15 to 330.15) K

By the gravimetric method, the solubility of dimethyl terephthalate (DMT) is measured in eight monary solvent and one binary solvent (methanol-ethanol) at atmospheric pressure from T = (298.15 to 330.15) K. Obviously, the increase of temperature promotes the increase of solubility in each solvent, while the increase of methanol composition leads to the opposite result in methanol-ethanol. Simultaneously, associating the measured data with seven solid-liquid equilibrium models separately to obtain model parameters, the correlation results show that measured data and calculated data are in good consistence. Especially, the Apelblat and λh have the best agreements for the monary and binary solvents respectively. Furthermore, thermodynamic parameters such as entropy, enthalpy and Gibbs free energy of DMT dissolution are acquired, which proves that the DMT dissolution is an enthalpy-driven, irreversible and endothermic process in every tested solvents.

QbD-Driven Analytical Method Development and Validation for Raloxifene Hydrochloride in Pure Drug and Solid Oral Dosage Form

The present research work encompasses systematic development of a simple, fast, sensi- tive, reproducible and cost-effective reversed-phase high-performance liquid chromatographic (RP-HPLC) method, applying the principles of Quality by design (QbD) for quantification of raloxifene hydrochloride (RLX). Reversed-phase chromatography was carried out using Waters Acquity high-performance liquid chro- matographic system (HPLC), equipped with photodiode array (PDA) detector controlled by Empower 2® software, using a Hibar Purospher® STAR C18 (250 x 4.6 mm; 5 μm) HPLC column. Initial risk assessment followed by screening studies was carried out using a four-factor-eight-run fractional factorial design (FFD). Subsequently, optimization studies were carried out employing a central composite design (CCD) with flow rate and mobile phase ratio as the critical method parameters (CMPs), and tailing factor (TF), % assay and theoretical plate count (TPC) as the critical analytical attributes (CAAs). The optimal chromatographic sepa- ration was conducted using graphical and numerical optimization with the mobile phase composition of methanol and sodium acetate buffer (pH 4) in the ratio of 50:50 (% v/v) at a flow rate of 1mL/min, an oven temperature of 40°C at a λmax of 287 nm. Linearity was observed in the concentration range of 0.5-100 μg/mL, with a correlation coefficient of 0.999. Further, the LOD and LOQ were found to be 0.5432 ng/mL and 1.6461 ng/mL respectively. The developed method was validated as per the ICH Q2 (R1) guidelines, and the principles and science of analytical quality by design (AQbD) for establishing a high degree of linearity, accuracy, precision, sensitivity and robustness, for estimating RLX in bulk drugs and marketed formulations.

Analysis of Wild Raspberries (Rubus idaeus L.): Optimization of the Ultrasonic-Assisted Extraction of Phenolics and a New Insight in Phenolics Bioaccessibility

A simple and efficient ultrasonic-assisted extraction (UAE) technique was developed in order to find optimal conditions for the extraction of total phenolic compounds, flavonoids and anthocyanins in wild raspberry (Rubus idaeus L.) fruits. Several extraction variables, including methanol composition (v/v, %), solid-solvent ratio (g/mL), time (min) and extraction temperature (°C) were optimized using response surface methodology (RSM). Under optimal conditions for extraction, the total phenolics were found in the concentration of 383mg GAE/100g of fresh fruit weight, while HPLC-PDA analysis of the optimized extract showed the presence of cyanidin-3-glucoside, cyanidin-3-sophoroside, catechin, gallic and ellagic acid. The experimental values of DPPH and ABTS radical scavenging activities were 29.0 and 39.5μmol Trolox/g of fresh fruit weight, respectively. In vitro simulated gastrointestinal digestion showed great raspberry phenolics stability. Our study assessed the bioaccessible phenolics in wild raspberry fruits and showed optimal conditions for the effective extraction of bioactive compounds for their analysis.

A A VALIDATED REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-CHARGED AEROSOL DETECTOR TECHNIQUE FOR THE SIMULTANEOUS ESTIMATION OF SITAGLIPTIN AND ERTUGLIFLOZIN IN PURE AND PHARMACEUTICAL DOSAGE FORMS

Objective: The main objective of the present work is to develop and validate a selective reverse-phase (RP) high-performance liquid (HPLC)-charged aerosol detection technique for the quantitation of the sitagliptin and ertugliflozin in dosage form to attain high degree of sensitivity.
 Materials and Methods: In present HPLC technique, separation of drugs was achieved on Phenomenex C18 column (250×4.6 mm, 5 μ) with a mobile phase composition of phosphate buffer (pH – 5.8), acetonitrile, and methanol in the proportion of 40:40:20%V/V. 1 ml/min flow rate and 256 nm wavelength detection were maintained for the elution of drugs in the chromatographic system. The retaining time of sitagliptin and ertugliflozin in column was found to be 4.2 and 2.4 min, respectively.
 Results: The projected technique was successfully applied for the quantitation of sitagliptin and ertugliflozin as a single combined mixture. The linearity statistics for calibration curves shown a good linearity in the concentration range of 0.3125–10 μg/ml for sitagliptin and 0.0625–2.5 μg/ml for ertugliflozin. The average values of regression coefficient, slope, and intercept were 0.9998, 8688.2, and 1977.6 for sitagliptin and 0.9996, 33602, and 1852.6 for ertugliflozin. The technique was validated as per the International Council for Harmonization guidelines. The limit of detection and limit of quantification findings were 0.082 and 0.247 μg/ml for sitagliptin and 0.04 and 0.12 μg/ml for ertugliflozin.
 Conclusion: The developed and validated RP-HPLC-charged aerosol detector technique of sitagliptin and ertugliflozin in dosage form showed that the method was accurate and selective with high degree of sensitivity.

Alternative Ultrasound-Assisted Method for the Extraction of the Bioactive Compounds Present in Myrtle (Myrtus communis L.).

The bioactive compounds in myrtle berries, such as phenolic compounds and anthocyanins, have shown a potentially positive effect on human health. Efficient extraction methods are to be used to obtain maximum amounts of such beneficial compounds from myrtle. For that reason, this study evaluates the effectiveness of a rapid ultrasound-assisted method (UAE) to extract anthocyanins and phenolic compounds from myrtle berries. The influence of solvent composition, as well as pH, temperature, ultrasound amplitude, cycle and solvent-sample ratio on the total phenolic compounds and anthocyanins content in the extracts obtained were evaluated. The response variables were optimized by means of a Box-Behnken design. It was found that the double interaction of the methanol composition and the cycle, the interaction between methanol composition and temperature, and the interaction between the cycle and solvent-sample ratio were the most influential variables on the extraction of total phenolic compounds (92.8% methanol in water, 0.2 s of cycle, 60 °C and 10:0.5 mL:g). The methanol composition and the interaction between methanol composition and pH were the most influential variables on the extraction of anthocyanins (74.1% methanol in water at pH 7). The methods that have been developed presented high repeatability and intermediate precision (RSD < 5%) and the bioactive compounds show a high recovery with short extraction times. Both methods were used to analyze the composition of the bioactive compounds in myrtle berries collected from different locations in the province of Cadiz (Spain). The results obtained by UAE were compared to those achieved in a previous study where microwave-assisted extraction (MAE) methods were employed. Similar extraction yields were obtained for phenolic compounds and anthocyanins by MAE and UAE under optimal conditions. However, UAE presents the advantage of using milder conditions for the extraction of anthocyanins from myrtle, which makes of this a more suitable method for the extraction of these degradable compounds.

Design of experiment avenue for development and validation of RP-HPLC-PDA method for determination of apremilast in bulk and in in-house tablet formulation

BackgroundApremilast is phosphodiesterase-4 and an immunomodulating agent used for treatment of refractory psoriatic arthritis.MethodsThe reversed-phase high-performance liquid-chromatography method for analysis of apremilast was developed and validated as per ICH guidelines. The separation of apremilast was performed on PrincetonSPHERE Ultima C18 column (250 mm × 4.6 mm, i.d., 5 μm particle size) with photodiode array detection carried out at 231 nm. A Box–Behnken design with response surface methodology was executed out for optimization of chromatographic conditions of reversed-phase high-performance liquid-chromatography for finished desired chromatographic separation of apremilast from its formulation with less number of experimental trials. Three independent factors, namely methanol composition in the mobile phase, pH of an aqueous phase, and flow rate, were used to construct a mathematical model and study the effects of these independent factors on responses such as retention time, theoretical plates, and tailing factor.ResultsOptimized experimental conditions for proposed work consists of methanol and water, pH 3.50 adjusted with ortho-phosphoric acid (70:30 % v/v) as a mobile phase at a flow rate 1 ml/min with a retention time was found to be 5.15 min. Accuracy study was completed at three different levels and was found in the range of 99.44–101.49%.ConclusionThe 3D response surface graphs revealed that the methanol composition and pH of an aqueous phase were both most stringent factors affecting the responses. Thus, a new, precise, and accurate HPLC method was developed and validated and can be used for regular analysis of apremilast.

Determination of ospemifene in human plasma by LC-MS/MS and its application to a human pharmacokinetic study.

A highly sensitive, specific and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method has been developed and validated for the determination of ospemifene in human plasma using ospemifene-d4 as an internal standard. Solid-phase extraction technique with Phenomenex Strata X-33 μm polymeric sorbent cartridges (30 mg/1 mL) was used to extract the analytes from the plasma. The chromatographic separation was achieved on Agilent Eclipse XDB-Phenyl, 4.6 × 75 mm, 3.5 μm column using the mobile phase composition of methanol and 20 mm ammonium formate buffer (90:10, v/v) at a flow rate of 0.9 mL/min. A detailed method validation was performed as per the US Food and Drug Administration guidelines and the calibration curve obtained was linear (r2 = 99) over the concentration range 5.02-3025 ng/mL. The API-4500 MS/MS was operated under multiple reaction monitoring mode during the analysis. The proposed method was successfully applied to a pharmacokinetic study in healthy human volunteers after oral administration of an ospemifene 60 mg tablet under fed conditions.

Phytochemical profiling, antioxidant, enzyme inhibition and cytotoxic potential of Bougainvillea glabra flowers

In this study, phytochemical composition, antioxidant, enzyme inhibition and cytotoxic activities of methanol and dichloromethane (DCM) extracts of Bougainvillea glabra (B. glabra) flowers were investigated. Methanol extract was found to have higher total bioactive contents and UHPLC-MS analysis of methanol extract revealed the presence of well-known phenolic and flavonoid compounds. Antioxidant activities were performed by radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), phosphomolybdenum (TAC) and metal chelating assays. From our result, we observed that methanol extract had many antioxidant compounds. The DCM extract exhibited higher cholinesterases and α-glucosidase enzyme inhibition, while methanol extract showed significant urease inhibition. Both extracts exhibited strong to moderate cytotoxicity against MCF-7, MDA-MB-231, CaSki, DU-145 and SW-480 cancer cells with IC50 values ranging from 88.49 to 304.7 µg/mL. The findings showed the B. glabra to possess considerable antioxidant, enzyme inhibition and cytotoxic potentials and therefore has potential to discover novel bioactive molecules.

DoE Approach: A validated Stability Indicating RP-HPLC Method Development for the Separation of Diasteromeric Analogs and Process Impurities of Carfilzomib

Abstract A novel reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the determination and separation of thirty diastereomers and six process related impurities of Carfilzomib (CFZ) active ingredient using YMC-Pack ODS-A (150 mm x 4.6 mm, 3µm) column and mobile phase comprising of pH 5.5 potassium di-hydrogen phosphate buffer, acetonitrile and methanol. The flow rate and detection wavelength were 0.9mL min−1 and 220 nm respectively. Design of Experiment (DOE) approach was strategically designed to optimize the critical chromatographic parameters like column temperature, flow rate and composition of methanol in the mobile phase to achieve the separation of diastereomers and process impurities. The developed method was validated for linearity, range, precision, accuracy, specificity, selectivity and intermediate precision.

In vitro biological propensities and chemical profiling of Euphorbia milii Des Moul (Euphorbiaceae): A novel source for bioactive agents

The plants of Euphorbia genus have been extensively studied for their nutritive and therapeutic purposes. The present research is the foremost effort to investigate and compare the biological activities and chemical composition of dichloromethane (DCM) and methanol (MeOH) solvent extracts of Euphorbia milli Des Moul aerial and root parts. Antioxidant potential was determined using six different (FRAP, CUPRAC, Phosphomolybdenum, DPPH, ABTS and ferrous chelation) methods. The enzyme inhibition effects of the tested extracts were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glucosidase, α-amylase and tyrosinase. Similarly, the amount of total phenolic and flavonoid contents were assessed via spectrophotometric methods and individual secondary metabolites were also determined using UHPLC-MS analysis. Methanolic extracts from both aerial and root parts contained the highest contents for phenolic and flavonoids which tends to correlate with their significant DPPH, ABTS (radical scavenging), FRAP, CUPRAC (reducing power) and α-glucosidase inhibition potentials. While, both the DCM extracts containing the lowest bioactive contents were most active in the phosphomolybdenum assay, cholinesterases and tyrosinase inhibition. The root extracts proved to be a better source of bioactive antioxidant molecules. Additionally, UHPLC-MS profiling of both the methanolic extracts revealed the presence of total 16 secondary metabolites belonging to five major groups (phenolic, flavonoid, coumarin, glycoside and alkaloid). To conclude, our results suggest that E. milii can be considered as a promising lead origin for natural bioactive enzyme inhibitory and antioxidant compounds which could pave the way for industrial applications.

Solvent-induced modulation of the chemical sensing performance of gold nanoparticle film chemiresistors

The analyte sensitivity of a chemical sensor is commonly tuned by the type of chemical recognition element. Here, it is shown that the solvent also plays a critical role in the sensing performance of thiol-capped gold nanoparticle film chemiresistors. Both the resistance change and speed of response of the chemiresistor can be optimised by adjusting the composition of methanol and water used for detection of the pesticide permethrin. It is shown that the resistance change is governed by the partitioning of the analyte between the thiol coating of the gold nanoparticles and the solvent. The partition coefficient, log P, has a strong influence on the response magnitude and is directly correlated to the response time. By varying the solvent, the log P of extremely high partitioning analytes such as permethrin in water can be lowered, and a much larger resistance change can be obtained in a shorter time period (5 min). A theoretical model is developed that correlates the experimental findings to changes in analyte partition coefficient with solvent type and composition. The results have significant implications for the design of chemical sensors and solvent conditions that can offer an enhanced signal within a practical timeframe.

Solubility modelling and thermodynamic aspect of 2,3-dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone in binary aqueous solutions of several alcohols

The equilibrium solubility of 2,3-dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone in four neat solvents namely methanol, ethanol, n-propanol and isopropanol and co-solvent mixtures of methanol (1) + water (2), ethanol + water (2), n-propanol (1) + water (2) and isopropanol (1) + water (2) at temperature range from (278.15 to 333.15) K was reported. The experiment was carried out by the saturation shake-flask technique under atmosphere pressure (101.1 kPa). At the same composition of methanol, ethanol, n-propanol or isopropanol and temperature, the mole fraction solubility of 2,3-dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone was highest in methanol (1) + water (2) mixtures, and lowest in isopropanol (1) + water (2) mixtures. By using the Jouyban-Acree model, van’t Hoff-Jouyban-Acree model and Apelblat-Jouyban-Acree model, 2,3-dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone solubility was well correlated obtaining RAD lower than 2.65% and RMSD lower than 1.85 × 10−4. The transfer Gibbs free energy (ΔtrG°), enthalpy (ΔtrH°), and entropy (ΔtrS°) were calculated, demonstrating that the solubilization capacity was more favorable with the increase in the alcohol concentration. Furthermore, quantitative values for the local mole fraction of methanol (ethanol, n-propanol or isopropanol) and water around the 2,3-dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone were computed by using the Inverse Kirkwood–Buff integrals method applied to the determined solubility data. 2,3-Dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone was preferentially solvated by water for the four solvent mixtures in water-rich compositions; while in intermediate and co-solvent-rich composition for alcohol (1) + water (2) mixtures, 2,3-dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone is preferentially solvated by the alcohol. The preferential solvation magnitude of 2,3-dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone was highest in isopropanol mixtures than in the other three co-solvent mixtures.

Development of New Analytical Microwave-Assisted Extraction Methods for Bioactive Compounds from Myrtle (Myrtus communis L.).

The phenolic compounds and anthocyanins present in myrtle berries are responsible for its beneficial health properties. In the present study, a new, microwave-assisted extraction for the analysis of both phenolic compounds and anthocyanins from myrtle pulp has been developed. Different extraction variables, including methanol composition, pH, temperature, and sample–solvent ratio were optimized by applying a Box–Behnken design and response surface methodology. Methanol composition and pH were the most influential variables for the total phenolic compounds (58.20% of the solvent in water at pH 2), and methanol composition and temperature for anthocyanins (50.4% of solvent at 50 °C). The methods developed showed high repeatability and intermediate precision (RSD < 5%). Both methods were applied to myrtle berries collected in two different areas of the province of Cadiz (Spain). Hierarchical clustering analysis results show that the concentration of bioactive compounds in myrtle is related to their geographical origin.

Ionic liquid modified multiwalled carbon nanotube embedded styrene butadiene rubber membranes for the selective removal of toluene from toluene/methanol mixture via pervaporation

Abstract Present work reports the fabrication of elastomeric membrane based on ionic liquid functionalized multiwalled carbon nanotubes (f-MWCNT) incorporated Styrene butadiene rubber (SBR) for the separation of azeotropic composition of toluene and methanol. The fabricated membranes were characterized by morphological analysis using transmission electron microscopy and glass transition temperature and heat capacity measurements by differential scanning calorimetry. Composite membranes demonstrate impressive separation performance with preferential selectivity towards toluene and 5 phr f-MWCNT loaded membranes was found to show the best result with respect to toluene flux and selectivity. Optimal separation performance with the permeation flux 225% of SBR control membrane and separation factor of 128 (1.6 times of SBR control membrane) is obtained for this membrane. The concurrent optimization of the physical and chemical structures of toluene permeation path on f-MWCNT surface provides the membrane with high-efficiency toluene permeation. Ionic liquid on MWCNT surface confer aromatic pi-pi interaction with toluene molecules leading to greater toluene affinity and higher repellency against methanol. Pervaporation characteristics of the membranes were also strongly influenced by the feed mixture composition. The study confirmed that increasing toluene concentration improved the toluene flux but reduced the separation factor. The experimental pervaporation fluxes were compared with the calculations based on modified Maxwell–Stefan equation. The model allows a good quantitative prediction of experimental flux values.

Biodiesel production by transesterification in two steps: Kinetic effect or shift in the equilibrium conversion?

In continuous biodiesel production plants, practically all technologies carry out the transesterification in two steps, feeding methanol and catalyst in each of them. The glycerine phase is separated after the first one. The explanation normally given to justify this procedure is that the glycerine separation after the first step shifts the equilibrium towards products, thus increasing the conversion. The reacting mixture is a system with partial miscibility, being the compositions of the biodiesel and glycerine phases different depending upon the global composition of methanol, catalyst, soaps, water, etc. The results presented in this work make it possible to conclude that the most important cause of the conversion improvement when performing the reaction in two steps is a change in the catalyst and methanol distribution between the biodiesel and glycerine phases. The separation of the glycerine phase leads to a positive impact on the kinetic of the second reaction step, since it allows that both the catalyst and the methanol concentrate in the biodiesel phase. This is due to the small quantity of glycerine phase present in the system in the second reaction step. Therefore, the higher concentrations of catalyst and methanol in the biodiesel phase significantly increase the rate of transesterification and decrease the mass transfer limitations.

Sorption and Thermodynamic Characteristics of Compositions of Water-Soluble Nonionic Polymers and Nanocluster Polyoxomolybdates

New data on the interaction between methanol vapors and compositions based on polyvinylpyrrolidone and polyvinyl alcohol that include nanocluster polyoxomolybdates of the keplerate and toroidal types are obtained using the gravimetric option of equilibrated interval sorption and the results from calculations of the thermodynamic cycle. Adsorption isotherms, concentration dependences of the Gibbs energy of the interaction between methanol and polymer films, and the Gibbs energy and entropy for polyoxometallate–polymer systems are obtained. The data are analyzed from the viewpoint of the effect the nature of components has on the properties of the compositions.

Phenolic Composition, Antioxidant and Cytotoxic Prospective of three Linum species: A Potential Source of Novel Anticancer Pharmacophores

The therapeutic potential of some Linum species has been reported previously. Nonetheless, several species of this genus warrant further scientific consideration. In this study, the phenolic composition, antioxidant activity (DPPH, phosphomolybdenum, iron chelating, hydroxyl radical and lipid peroxidation assays) and cytotoxicity effect (human rhabdomyosarcoma, human cervix carcinoma, and murine fibroblast cell lines) of ethyl acetate, methanol, and water extracts of three Linum species (Linum austriacum subsp. glaucescens, Linum hirsutum subsp. anatolicum, and Linum tenuifolium) were assessed. High performance liquid chromatography with diode-array detection (HPLC-DAD) analysis revealed the presence of ferulic acid, p-hydroxybenzoic acid, synapic acid, rutin, apigenin-glucoside, quercetin, luteolin, and naringenin in all the extracts. It was observed that the extracts (IC50 ranging from 42.07 to 51.66 µg/mL) showed IC50 significantly (p<0.05) lower than ascorbic and gallic acid (> 1000 and 255.43 µg/mL respectively) against lipid peroxidation. According to the American National Cancer Institute guidelines, the extracts showed low cytotoxicity (IC50 < 30 µg/mL). However, the water extracts showed cytotoxicity effects (IC50 17.43±0.13 to 28.12±0.66 µg/mL) against the studied cell lines as compared to the antineoplastic positive control (Cis-DDP). These findings support the use of these plants in the management of ROS mediated disorders and advocates for the need for further scientific evaluations to confirm the observed in vitro bioactivity. The cytotoxic potential coupled with the antioxidant property of the water extracts of these plant species is of particular interest which can open new avenues in the quest for novel anticancer drugs. Keywords: Linum, antioxidant, phenolic, nutraceuticals, anticancer, cytotoxic, Turkey.