Cold Seeps Research Articles

Air Quality and Health Impacts of Onshore Oil and Gas Flaring and Venting Activities Estimated Using Refined Satellite-Based Emissions.

Emissions from flaring and venting (FV) in oil and gas (O&G) production are difficult to quantify due to their intermittent activities and lack of adequate monitoring and reporting. Given their potentially significant contribution to total emissions from the O&G sector in the United States, we estimate emissions from FV using Visible Infrared Imaging Radiometer Suite satellite observations and state/local reported data on flared gas volume. These refined estimates are higher than those reported in the National Emission Inventory: by up to 15 times for fine particulate matter (PM2.5), two times for sulfur dioxides, and 22% higher for nitrogen oxides (NOx). Annual average contributions of FV to ozone (O3), NO2, and PM2.5 in the conterminous U.S. (CONUS) are less than 0.15%, but significant contributions of up to 60% are found in O&G fields with FV. FV contributions are higher in winter than in summer months for O3 and PM2.5; an inverse behavior is found for NO2. Nitrate aerosol contributions to PM2.5 are highest in the Denver basin whereas in the Permian and Bakken basins, sulfate and elemental carbon aerosols are the major contributors. Over four simulated months in 2016 for the entire CONUS, FV contributes 210 additional instances of exceedances to the daily maximum 8-hr average O3 and has negligible contributions to exceedance of NO2 and PM2.5, given the current form of the national ambient air quality standards. FV emissions are found to cause over $7.4 billion in health damages, 710 premature deaths, and 73,000 asthma exacerbations among children annually.

Experimental study on gas slippage effect in coal at different temperatures based on nuclear magnetic resonance

Coalbed methane (CBM) can be recovered more effectively through heat injection mining. The slippage effect in coal becomes more significant as CBM is exploited, essential for maintaining consistent production of CBM wells. By “slippage effect,” we mean that gas molecules exhibiting non–zero velocity near the wall surface of coal pores, leading to higher gas permeability than liquid permeability. To explore the slippage effect and its control mechanism during heat injection mining, methane seepage experiments with constant effective stress were conducted under five different temperatures and seven pore pressures. An analysis was conducted on the mechanisms by which pore pressure and temperature affect gas slippage effect. Furthermore, the correlation between coal pore structure and the gas slippage effect was revealed using the nuclear magnetic resonance (NMR) technique. The results appear that, first, as pore pressure increases, the slippage effect is suppressed due to the influence of the average molecular free path, despite the positive impact of adsorption–induced matrix expansion. Second, as temperature rises, the average molecular free path and thermal expansion have a positive impact on gas slippage, while matrix shrinkage caused by desorption has a negative impact. The positive impact is stronger, resulting in a continuous enhancement of the slippage effect. Finally, a new slippage factor calculation based on NMR T2 distribution of micropores and transitional pores (<100 nm, T2 < 8.33 ms) was provided, and the method was verified by slippage factor fitted by permeability experimental data. The heat injection mining of CBM can be theoretically guided by the research results.

Optimizing unconventional gas extraction: The role of fracture roughness

In unconventional reservoir engineering, such as coalbed methane and shale gas extraction, fracture behavior is pivotal in gas accumulation, migration, and extraction, acting as a primary channel for gas flow. Current research inadequately addresses the quantitative impact of fracture roughness on gas extraction. In this study, we introduce a novel interdisciplinary model that quantitatively characterizes shale fracture roughness and correlates it with shale permeability. This model comprehensively considers factors affecting shale extraction, including fracture roughness, in situ stress, reservoir deformation, and adsorption-desorption dynamics. It provides a thorough analysis of how fracture roughness influences gas seepage, extraction efficiency, and reservoir stability under various physical conditions. Our validated findings reveal that fracture roughness significantly affects shale permeability, stress responses, and displacement. As the fracture roughness coefficient χ increases from 0.2 to 1.0, the maximum reduction in gas pressure within the hydraulically fractured region is 4.7%, while the maximum increase in reservoir stress is 2.8%. In shale reservoirs near extraction well, particularly in hydraulically fractured zones, the maximum decrease in shale gas pressure is 11.1%, and the maximum increase in stress is 3.2%, which offer a groundbreaking approach for optimizing extraction rates and ensuring project safety in the industry.

New Phenol Derivatives from the Haima Cold Seep-Derived Fungus Aspergillus subversicolor CYH-17.

Seven new phenol derivatives, subversins A-E (1-5), subversic acid A (6) and epi-wortmannine G (7); one new natural product, 4-hydroxy-7-methoxyphthalide (8); and five known compounds (9-13) were isolated from the fungus Aspergillus subversicolor CYH-17 collected from the Haima cold seep. The structures and absolute configurations of these compounds were determined via NMR, MS, optical rotation, electronic circular dichroism (ECD) calculation, X-ray diffraction analysis and comparison with the literature. Compounds 2 and 5 were two pairs of enantiomers. All compounds were tested for their α-glucosidase and acetylcholinesterase (AChE) inhibitory activity, antioxidant activity and antibacterial activity, but no obvious activity was observed among these studied compounds.

Pseudallenes A and B, new sulfur-containing ovalicin sesquiterpenoid derivatives with antimicrobial activity from the deep-sea cold seep sediment-derived fungus Pseudallescheria boydii CS-793.

Pseudallenes A and B (1 and 2), the new and rare examples of sulfur-containing ovalicin derivatives, along with three known analogues 3-5, were isolated and identified from the culture extract of Pseudallescheria boydii CS-793, a fungus obtained from the deep-sea cold seep sediments. Their structures were established by detailed interpretation of NMR spectroscopic and mass spectrometric data. X-ray crystallographic analysis confirmed and established the structures and absolute configurations of compounds 1-3, thus providing the first characterized crystal structure of an ovalicin-type sesquiterpenoid. In the antimicrobial assays, compounds 1-3 showed broad-spectrum inhibitory activities against several plant pathogens with MIC values ranging from 2 to 16 μg/mL.

Deep-sea in situ and laboratory multi-omics provide insights into the sulfur assimilation of a deep-sea Chloroflexota bacterium.

The cycling of sulfur is one of Earth's major biogeochemical processes and is closely related to the energy metabolism of microorganisms living in the deep-sea cold seep and hydrothermal vents. To date, some of the members of Chloroflexota are proposed to play a previously unrecognized role in sulfur cycling. However, the sulfur metabolic characteristics of deep-sea Chloroflexota bacteria have never been reported, and remain to be verified in cultured deep-sea representatives. Here, we show that the deep-sea Chloroflexota bacterium ZRK33 can perform sulfate assimilation in both laboratory and deep-sea conditions, which expands our knowledge of the sulfur metabolic potential of deep-sea Chloroflexota bacteria. We also show that the genes associated with assimilatory and dissimilatory sulfate reduction ubiquitously distribute in the deep-sea Chloroflexota members, providing hints to the roles of Chloroflexota bacteria in deep-sea sulfur biogeochemical cycling.

Trace and Rare Earth Element Fingerprints of Aerobic Oxidation of Methane in Seep‐Dwelling Bivalves

AbstractAerobic methanotrophic bacteria are pivotal in the global carbon cycle by converting methane into biomass and inorganic carbon species. Light rare earth elements (light‐REE; La, Ce) are part of the metalloenzymes mediating the biochemical processes in methanotrophs. However, the partitioning of trace metals and REE in chemosymbiotic megafauna with methanotrophic endosymbionts remains largely unknown. Here we determine stable isotope compositions (δ13C, δ15N) of soft tissues (gill, mantle, foot) as well as trace metal and REE contents of soft tissues and shells of chemosymbiotic bivalves dwelling at methane seeps (Site F and Haima seeps) of the South China Sea. Isotopic compositions of soft tissues are found to reflect the mode of chemosymbiosis (i.e., methanotrophy vs. thiotrophy). Particularly gill tissues of bivalves with methanotrophic endosymbionts display marked light‐REE and trace metal enrichments, which is interpreted to reflect the enzymatic activity of endosymbionts. Additionally, correlations between light‐REE and copper (Cu), zinc (Zn), and molybdenum (Mo) contents in soft tissues of chemosymbiotic mussels are ascribed to the uptake of these elements by the methanotrophic symbionts. An observed higher trace metal content in the tissue of the semi‐infaunal and infaunal bivalves with thiotrophic endosymbionts is believed to reflect the uptake of metals associated with sulfide particles. This study documents diagnostic enrichment of trace metals and light‐REE in the soft tissues of bivalves with chemotrophic endosymbionts and provides new constraints for future identification of chemosymbiosis at ancient seeps.

Structural control on carbon emissions at the Nirano mud volcanoes – Italy

The Nirano Salse in Italy is a well-studied site where natural gas seepage (NGS) and other hydrocarbon fluids and gases are emitted at the earth's surface. A novel integrated approach is applied to define a comprehensive structural interpretation of the gas seepage and flow dynamic in the mud volcano system of the Nirano Salse Regional Nature Reserve (Modena, Northern Apennines). The paper investigates the relationship between gas emissions and local structures, particularly faults and fractures, in the shallow subsurface (down to 500–600 m depth) to understand the control that structures have on fluid ascent from deep leaky hydrocarbon traps. We performed continuous monitoring of mud levels within vents; carried out geological surveys to characterize the main stratigraphic and structural discontinuities; measured the carbon emissions (CH4 and CO2) seepage both from volcanoes and the surrounding soil by a portable gas fluxmeter; and integrated the results with available geophysical surveys. The authors argue that the transgressive Pleistocene-Pliocene Argille Azzurre Formation hides the complex and highly structured pre-Pliocene geology of the area, in which faults and fractures act as pathways for deep fluid ascent. The emissions are aligned along a NE-SW trend at the intersection of a NE-SW fracture system and NW-SE-oriented normal faults, which are both associated to the local tensional stress field of a likely left-lateral strike-slip transfer structure or in the extrados of a fold. By examining both natural gas macroseepage and diffuse flux, it is shown that local structures control the fluid ascent and contribute to the emission of hydrocarbon gases and fluids at the Earth's surface. Understanding the structural control of carbon emissions at the Nirano Salse is also important for evaluating the carbon budget at the site, particularly in areas where there are detectable surface emissions. The study has implications for geologic, environmental, and economic issues, including hydrocarbon exploration, hazard assessment, and impact on the atmospheric carbon budget. Furthermore, the outcomes have an important implication to evaluate the potential for dangerous abrupt mud eruptions, and the site safety in proximity to the mud volcanoes.

Trophic relationship between mussels and scale worms under various seepage intensities in the haima cold seep: Insights from stable isotopes (δ13C and δ15N) and C:N:P stoichiometry

The deep-sea mussels Gigantidas haimaensis (Mytilidae: Bathymodiolinae) usually contain one scale worm Branchipolynoe pettiboneae in their mantle cavity in the Haima cold seep, South China Sea. To explore their environmental adaptation and coexistence mechanisms, the stable isotopes (δ13C and δ15N) and C:N:P ecological stoichiometry of G. haimaensis and the associated B. pettiboneae were investigated under different methane seepage intensities. In the presence of seepage, most mussels harbored one scale worm in their mantle cavity. However, under seepage cease, the physiological status of mussels looked unhealthy, and no scale worm appeared in their bodies. The variation in δ13C values was great among different mussel tissues, ranging from −49.7‰ to −57.8‰. The δ13C values of mussel tissues followed the order of foot > gill > mantle under active seepage, while no regular trend was found under seepage cease. The δ13C and δ15N of scale worms were averagely enriched by 2.9 ‰ and 3.2 ‰ relative to their mussel hosts, and the trophic niche separation between scale worms and mussels was more significant under active seepage. The δ13C value of mussel foot was significantly higher under active seepage (average −50.8‰) than under seepage cease (average −55.1‰), indicating that mussels might ingest and assimilate more 13C-rich suspended particulate organic matter (POM) under active seepage. In mantle tissues, the high C:N ratio and low δ13C value should be attributed to the high content of energy storage substances. The C:N ratio of mantle dramatically declined with the exhaustion of energy storage materials under seepage cease. The variation of P content was most significant in the gill, which might be regulated by the abundance of symbiotic bacteria. It can be speculated that the dramatic decline of P content in the gill was attributed to the loss of high-P bacterial symbionts under seepage cease. Both the C:P and N:P ratios of gill tissues significantly increased under seepage cease, which suggested the decline of food quality for scale worms. Mussel-dwelling scale worms might detect the change of food quality and abandon their starving mussel host. Our results help to better understand the environmental adaptation of chemoautotrophic mussels and scale worms under unstable seepage intensity in cold seep ecosystems.

Investigation on damage-permeability model of dual-porosity coal under thermal-mechanical coupling effect

Understanding the permeability characteristics of coal under mining disturbance is crucial for ensuring coal mine safety and efficient gas extraction. Coal is a dual porosity medium consisting of matrix and fracture, and there are some differences in mechanics and permeability between matrix pores and fracture. However, research on the damage and gas seepage characteristics of dual-porosity coal is limited. In this paper, a coupled damage-permeability model considering matrix pores and fractures in coal is established, incorporating the influences of effective stress, gas adsorption, thermal expansion, and thermal cracking. And the reliability of the newly developed model was verified using experimental data of temperature and pressure variations under different stress boundary conditions. The results indicate that during the elastic stage, the total permeability and fracture permeability of coal exhibit a trend of decreasing and then increasing with pore pressure, while matrix permeability gradually increases. In the full stress-strain stage, the overall damage-permeability and fracture damage-permeability of coal exhibit an “S"-shaped variation trend with axial strain, while matrix damage-permeability shows a slow increasing trend. To accurately characterize the evolution of gas seepage in coal, contributions from matrix pores and fractures must be comprehensively considered, and the effects of gas adsorption, effective stress, thermal cracking, and thermal expansion on permeability cannot be ignored. Furthermore, through parameter sensitivity analysis, it is found that during the elastic stage, coal permeability decreases with an increase in the adsorption sensitivity coefficient and thermal expansion coefficient. In the full stress-strain process, coal permeability increases with an increase in the damage-permeability coefficient. The results of this study are of great significance for predicting the evolution of coal permeability in the working face ahead under mining disturbance.

Exploring the depths: Metagenomic profiling of cold seep bacterial communities and isolation of hydrocarbon-degrading microbes from the Cauvery-Mannar basin

Cold seeps are unique ecosystems characterized by the presence of endemic communities reliant on microorganisms that survive through chemosynthesis, utilizing sulfide, methane, hydrocarbons, and other reduced compounds. Recently, a cold seep was discovered in the Cauvery-Mannar basin during an expedition aboard the research vessel ORV Sindhu Sadhana which led to the investigation into the distribution and isolation of microorganisms within the sediment layers (0–6 cm). Metagenomic analysis based on V3-V4 region of the sediment's bacterial community revealed a prevalence of bacteria associated with methane and hydrocarbons. Dominant phyla included Campilobacterota (20–25%), Proteobacteria (15–25%), Firmicutes (15-20%), Desulfobacterota (10–18%), Chloroflexi (5–10%), and Actinobacterota (5–7%). In-vitro isolation of hydrocarbon-degrading bacteria using minimal media supplemented with 1% crude oil gave rise to 15 bacterial strains. Molecular identification of six isolates (chosen based on the dense growth obtained in agar plates) revealed their affiliation with the genera Acinetobacter, Rhodococcus, Acinetobacter, and Bacillus. This comprehensive study constitutes the first report on the bacterial community structure found in sediments from a cold seep of Cauvery-Mannar basin, along with the successful isolation and identification of hydrocarbon-degrading bacteria.

Bioaccumulation and Trophic Transfer of Short-, Medium-, and Long-Chain-Chlorinated Paraffins in Benthic Biotas of the Haima Cold Seep, South China Sea

Bioaccumulation and Trophic Transfer of Short-, Medium-, and Long-Chain-Chlorinated Paraffins in Benthic Biotas of the Haima Cold Seep, South China Sea

Microeukaryotes have unexpected importance in cold seep food webs through predation and parasitism

Cold seeps are inhabited by a high abundance of chemosynthetic prokaryotes and invertebrates. However, a complete food web between invertebrates and prokaryotes cannot be established without the important link of microeukaryotes. Yet, question such as which microeukaryotes are at work in cold seeps is still mostly open. We, therefore, compared the biological communities in cold seeps and adjacent areas of the South China Sea by the metabarcoding and quantitative PCR (qPCR) of nuclear 18S and 16S rRNA genes, and estimated the potentially ecological roles of microeukaryotes by cross-domain microbial network analysis. The anaerobic methane oxidizing archaea (ANME), sulfate-reducing bacteria (SRB) and Radiolaria accounted for most variations between microbial communities from the cold seeps and adjacent areas. A significant correlation was detected between the copy numbers of 18S rRNA gene and communities of the ANME and SRB in cold seeps, indicating close links between microeukaryotes and chemosynthetic prokaryotes. After adding microeukaryotic nodes microbial network became more complex, and the negative correlation proportion and average path length increased, suggesting increased predation and parasitism. The ANME and SRB groups were mainly negatively correlated with and potentially preyed by Acantharea and Cercozoa, which were then negatively correlated with annelids and mollusks. Parasitic microeukaryotes of Syndiniales widely existed in cold seeps and had close relationships with Polycystinea. Parasitism might cause the death of Polycystinea and the release of Syndiniales spores, which may be an important carbon source for the cold seep biota. Using Mantel analyses, we also demonstrated that trophic relationships are closer in cold seeps than in the adjacently deep-sea sediments, and that microeukaryote-metazoans linkages in cold seeps are closer than those between prokaryotes and metazoans. Overall, our study outlines the main components of food webs in cold seeps and highlights that microeukaryotes have an unexpected importance in the carbon cycle.

Biogeochemical investigations of methane seepage at the ultraslow-spreading Arctic mid-ocean ridge: Svyatogor ridge, Fram Strait

The Arctic continental shelves are important reservoirs of methane stored in gas hydrates and deeper geological formations. However, little is known about methane dynamics in deeper oceanic settings such as mid-ocean ridges, mainly due to operational challenges related to their remoteness. This study investigates a recently discovered methane seepage environment at Svyatogor Ridge, a sediment-covered transform fault on the western flank of the Arctic mid-ocean ridge west of Svalbard. Svyatogor Ridge was previously hypothesized to host deep gas hydrates and active fluid flow systems, which is a unique combination for this setting and requires geochemical evidence. Based on sediment and foraminiferal geochemistry, we demonstrate that Svyatogor Ridge hosts a shallow methane cycle with anaerobic oxidation of microbial methane, sustaining chemosynthetic communities at the seafloor. Our geochemical datasets also suggest that methane fluxes were higher in the past than today and long-lasting, with episodes of intense methane oxidation recorded in pre-Holocene sediments. Methane seepage is still ongoing at this location, although no evidence exists that methane is reaching the sea surface. The results provide the first evidence of a methane seepage environment ever reported from the ultra-slow spreading Arctic mid-ocean ridge, thus calling for a reevaluation of the role of this type of ridge in the ocean methane cycle.

Characterizing the Oxygen Minimum Zone (OMZ) in the Costa Rican Eastern Tropical Pacific using in situ data from field campaigns

For conservation and sustainable fisheries, it is important to characterize the Oxygen Minimum Zones or OMZ in and around the methane seeps of the Eastern Tropical Pacific (ETP), Costa Rica, through the analysis of temperature, salinity, density, and oxygen profiles. The data used in this work were collected during several oceanographic research campaigns in the Pacific continental margin and offshore of Costa Rica, between 2009 and 2019, using a CTDs, as the profiler of physical parameters of the water column. In general, it was observed that dissolved oxygen gradually decreases with depth to the thermocline, then its concentration decreases more rapidly and remains low, indicating the presence of the OMZ and tends to increase slightly at greater depths. Mean vertical extension of the OMZ near and around the seeps was 763 m and the mean depth for the minimum dissolved oxygen value was 393 m. Spatial differences of measurements taken at stations near the methane seeps were calculated with respect to the measurements at the station located above them. Overall, a greater variability of the oxygen anomalies was observed within the mixed layer, while under the thermocline their values remain stable and around zero.

Monitoring underwater volcano degassing using fiber-optic sensing

Continuous monitoring of volcanic gas emissions is crucial for understanding volcanic activity and potential eruptions. However, emissions of volcanic gases underwater are infrequently studied or quantified. This study explores the potential of Distributed Acoustic Sensing (DAS) technology to monitor underwater volcanic degassing. DAS converts fiber-optic cables into high-resolution vibration recording arrays, providing measurements at unprecedented spatio-temporal resolution. We conducted an experiment at Laacher See volcano in Germany, immersing a fiber-optic cable in the lake and interrogating it with a DAS system. We detected and analyzed numerous acoustic signals that we associated with bubble emissions in different lake areas. Three types of text-book bubbles exhibiting characteristic waveforms are all found from our detections, indicating different nucleation processes and bubble sizes. Using clustering algorithms, we classified bubble events into four distinct clusters based on their temporal and spectral characteristics. The temporal distribution of the events provided insights into the evolution of gas seepage patterns. This technology has the potential to revolutionize underwater degassing monitoring and provide valuable information for studying volcanic processes and estimating gas emissions. Furthermore, DAS can be applied to other applications, such as monitoring underwater carbon capture and storage operations or methane leaks associated with climate change.

The diversification and potential function of microbiome in sediment-water interface of methane seeps in South China Sea.

The sediment-water interfaces of cold seeps play important roles in nutrient transportation between seafloor and deep-water column. Microorganisms are the key actors of biogeochemical processes in this interface. However, the knowledge of the microbiome in this interface are limited. Here we studied the microbial diversity and potential metabolic functions by 16S rRNA gene amplicon sequencing at sediment-water interface of two active cold seeps in the northern slope of South China Sea, Lingshui and Site F cold seeps. The microbial diversity and potential functions in the two cold seeps are obviously different. The microbial diversity of Lingshui interface areas, is found to be relatively low. Microbes associated with methane consumption are enriched, possibly due to the large and continuous eruptions of methane fluids. Methane consumption is mainly mediated by aerobic oxidation and denitrifying anaerobic methane oxidation (DAMO). The microbial diversity in Site F is higher than Lingshui. Fluids from seepage of Site F are mitigated by methanotrophic bacteria at the cyclical oxic-hypoxic fluctuating interface where intense redox cycling of carbon, sulfur, and nitrogen compounds occurs. The primary modes of microbial methane consumption are aerobic methane oxidation, along with DAMO, sulfate-dependent anaerobic methane oxidation (SAMO). To sum up, anaerobic oxidation of methane (AOM) may be underestimated in cold seep interface microenvironments. Our findings highlight the significance of AOM and interdependence between microorganisms and their environments in the interface microenvironments, providing insights into the biogeochemical processes that govern these unique ecological systems.

Deep-large faults controlling on the distribution of the venting gas hydrate system in the middle of the Qiongdongnan Basin, South China Sea

Many locations with concentrated hydrates at vents have confirmed the presence of abundant thermogenic gas in the middle of the Qiongdongnan Basin (QDNB). However, the impact of deep structures on gas-bearing fluids migration and gas hydrates distribution in tectonically inactive regions is still unclear. In this study, the authors apply high-resolution 3D seismic and logging while drilling (LWD) data from the middle of the QDNB to investigate the influence of deep-large faults on gas chimneys and preferred gas-escape pipes. The findings reveal the following: (1) Two significant deep-large faults, F1 and F2, developed on the edge of the Songnan Low Uplift, control the dominant migration of thermogenic hydrocarbons and determine the initial locations of gas chimneys. (2) The formation of gas chimneys is likely related to fault activation and reactivation. Gas chimney 1 is primarily arises from convergent fluid migration resulting from the intersection of the two faults, while the gas chimney 2 benefits from a steeper fault plane and shorter migration distance of fault F2. (3) Most gas-escape pipes are situated near the apex of the two faults. Their reactivations facilitate free gas flow into the GHSZ and contribute to the formation of fracture - filling hydrates.©2024 China Geology Editorial Office.

Evolution of Permeability and Gas Seepage in Deep Coal Under 3D Stress

Evolution of Permeability and Gas Seepage in Deep Coal Under 3D Stress

Potential Underestimate in Reported Bottom-up Methane Emissions from Oil and Gas Operations in the Delaware Basin

Methane is a greenhouse gas and identified as a key driver of near-term climate change. Bottom-up approaches estimate annual methane loss from US natural gas production and transport at 6 Tg, but recent studies suggest this may be an underestimate. To investigate this possibility, an equipment-based emissions inventory, using EPA emission factors, was developed to calculate methane emissions from oil and gas operations in the Delaware basin, USA. Emission factors and activity data were then updated using contemporary and region-specific measurement data. The original inventory estimated emissions at 315 Gg CH4 y−1 (gas production-normalized rate of 0.6% loss), while the updated inventory estimated emissions of 1500 Gg CH4 y−1 (2.8% loss). The largest changes resulted from large fugitive emissions from oil production (+430 Gg CH4 y−1), updating maintenance activity emissions (+214 Gg CH4 y−1), considering flaring inefficiency (+174 Gg CH4 y−1), and the inclusion of associated gas venting (+136 Gg CH4 y−1). This study suggests that a systematic underestimate probably exists in current bottom-up inventories and identifies sources currently missing or may be incorrect. We also strongly recommend that emission factors should be validated through direct comparison against measurement campaigns that include long-tail distributions typical of oil and gas activities.