Cold Seeps Research Articles

Genomic characterization of the bacterial phylum Candidatus Effluviviacota, a cosmopolitan member of the global seep microbiome.

The newly discovered bacterial phylum Candidatus Effluviviacota is widespread across diverse seepage ecosystems, marine environments, and freshwater environments, with a notable preference for cold seeps. While maintaining an average abundance of approximately 1% in the global gene catalog of cold seep habitats, it has not hitherto been characterized. The metabolic versatility of Ca. Effluviviacota in anaerobic carbon, hydrogen, and metal cycling aligns with its prevalence in anoxic niches, with a preference for cold seep environments. Variations in metabolic potential between Ca. Effluvivivax and Ca. Effluvibates may contribute to shaping their respective habitat distributions.

Quantitative analysis of the upwelling behavior of methane bubbles in nature and numerical simulations

In recent years, methane gas released from the seafloor has been considered a sub-oceanic resource, and comprehending the dynamics of methane bubbles in the ocean has garnered increasing attention. Therefore, in this study, we conducted a quantitative analysis of the behavior of ascending methane bubbles with and without a hydrate layer. The bubbles were filmed at two natural gas seep sites and reproduced by numerical simulations using two-dimensional motion analysis software. The simulations were performed with gas bubbles and methane hydrate (MH)1 bubbles spouting from a nozzle in a computational domain filled with pure water to assess the validity of image analysis for in-situ data, whereas numerical models and physical properties were utilized for the current two-phase (gas-liquid) simulations. The rising velocity, size, circumference, circularity, and maximum diameter of the methane bubbles were examined to understand the effects of the MH layer on the statistical and stochastic features of ascending methane bubbles. Based on the statistics of the above variables, the gas bubbles had a higher rising velocity and smaller circularity than the MH bubbles when the bubble sizes were identical. In addition, stochastic analysis indicated that the circularity of the MH bubble was uniquely determined by their size, due to the more rigid skin of the MH bubbles compared to that of gas bubbles. Consequently, discrepancies in bubble dynamics between methane gas and MH bubbles were clarified in this study.

Pore-fracture structures and seepage flow characteristics during spontaneous coal combustion based on CT 3D reconstruction

To study the evolution characteristics of the pore-fracture structures and seepage properties of coal in the process of spontaneous combustion, a high-temperature tube furnace was used to heat the coal samples. The coal samples were scanned using Xray-CT scanning technology, and the CT 3D reconstruction technology was used to extract the 3D pore structure and equivalent pore network model of each group of coal samples. Firstly, the specific surface area and volume of pore cleavage is statistically characterized; the change rules of Ep, Et, Lc, and Cn are quantitatively analyzed. At 200 °C, the coal body produces a large number of pore fractures, the development of coal samples is more significant, and the pore-specific surface area and the number of pore throats reached their maximum value. In addition, the changes of fractal dimension, porosity, connectivity, and permeability of coal with temperature were discussed, and the best permeability of the coal was achieved at 200 °C. The connectivity and permeability were weakened with the further increase of temperature, and the structural changes had a close influence on the gas seepage pressure and seepage flow rate. The results of the study provide important engineering guidance for deepening fire prevention in coalfields.

Analisa Cacat Produk Dan Kerusakan Mold Pada Proses Injection Molding Dan Tindakan Perbaikan Di PT. Patco Elektronik Teknologi

This study examines product defects and mold damage in the injection molding process and corrective actions at PT. Patco Elektronik Teknologi. This type of research is field research with a qualitative approach. Researchers use primary and secondary data sources. Data collection techniques through observation, interviews, and documents related to product defects and mold damage in the injection molding process and corrective actions. The data were analyzed using data reduction, data presentation, and conclusion drawing methods. The results of the study show that injection molding has many advantages when compared to other plastic manufacturing methods, but in the process there are various defects in the plastic products produced. Based on the FMEA analysis, a number of product defects occur due to several factors, such as Bending, Gas Mark, Silver, Flash, Under Cut, Crack, Flow Mark, Scratches, Burn Mark, and Short Mold. Gas Mark has the highest risk priority number (RPN) value of 75. The problem of defects due to gas marks occurs in the housing panel components produced by PT. Patco Elektronik Teknologi. The company took corrective action NG Gas Mark on the housing panel product by means of mold maintenance and gas vent making by the mold shop for the cavity and ejector pin parts. After the gas vent making process was carried out, defects due to gas marks were greatly reduced in the housing panel products produced by PT. Patco.

Promethearchaeum syntrophicum gen. nov., sp. nov., an anaerobic, obligately syntrophic archaeon, the first isolate of the lineage 'Asgard' archaea, and proposal of the new archaeal phylum Promethearchaeota phyl. nov. and kingdom Promethearchaeati regn. nov.

An anaerobic, mesophilic, syntrophic, archaeon strain MK-D1T, was isolated as a pure co-culture with Methanogenium sp. strain MK-MG from deep-sea methane seep sediment. This organism is, to our knowledge, the first cultured representative of 'Asgard' archaea, an archaeal group closely related to eukaryotes. Here, we describe the detailed physiology and phylogeny of MK-D1T and propose Promethearchaeum syntrophicum gen. nov., sp. nov. to accommodate this strain. Cells were non-motile, small cocci, approximately 300-750 nm in diameter and produced membrane vesicles, chains of blebs and membrane-based protrusions. MK-D1T grew at 4-30 °C with optimum growth at 20 °C. The strain grew chemoorganotrophically with amino acids, peptides and yeast extract with obligate dependence on syntrophy with H2-/formate-utilizing organisms. MK-D1T showed the fastest growth and highest maximum cell yield when grown with yeast extract as the substrate: approximately 3 months to full growth, reaching up to 6.7×106 16S rRNA gene copies ml-1. MK-D1T had a circular 4.32 Mb chromosome with a DNA G+C content of 31.1 mol%. The results of phylogenetic analyses of the 16S rRNA gene and conserved marker proteins indicated that the strain is affiliated with 'Asgard' archaea and more specifically DHVC1/DSAG/MBG-B and 'Lokiarchaeota'/'Lokiarchaeia'. On the basis of the results of 16S rRNA gene sequence analysis, the most closely related isolated relatives were Infirmifilum lucidum 3507LTT (76.09 %) and Methanothermobacter tenebrarum RMAST (77.45 %) and the closest relative in enrichment culture was Candidatus 'Lokiarchaeum ossiferum' (95.39 %). The type strain of the type species is MK-D1T (JCM 39240T and JAMSTEC no. 115508). We propose the associated family, order, class, phylum, and kingdom as Promethearchaeaceae fam. nov., Promethearchaeales ord. nov., Promethearchaeia class. nov., Promethearchaeota phyl. nov., and Promethearchaeati regn. nov., respectively. These are in accordance with ICNP Rules 8 and 22 for nomenclature, Rule 30(3)(b) for validation and maintenance of the type strain, and Rule 31a for description as a member of an unambiguous syntrophic association.

Computational Fluid Dynamics Simulation of Combustion Efficiency for Full-Size Upstream Flare Experiments

Methane emissions from oil and gas production can occur throughout the value chain, but for many producers, one of the most significant sources is flaring. Understanding the influence of the operating conditions and the environmental factors the combustion efficiency and destruction and removal efficiency (CE/DRE) of flares is essential if their role in methane emissions, a potent but short-lived greenhouse gas, is to be better understood and mitigated. An industry-scale experimental study was focused on the emissions of un-assisted flares commonly encountered in upstream oil and gas production. This paper simulates two un-assisted flare tips combustions by using the commercial computational fluid dynamics (CFD) software package Fluent 21R2 to augment the physical experimental testing. Two three-dimensional (3D) flare tips models are built, and the k-omega SST turbulence model and flamelet generated manifold (FGM) combustion model are applied to simulate flaring combustion. The CFD model is first validated against full-scale industry flare tests that use extractive sampling of the combustion plume. CFD results are in good agreement with measured results when the vent gas net heating value (NHV) is greater than 300 BTU/SCF. Greater uncertainty exists for both CFD results and measured data if the NHV is less than 300 BTU/SCF. Then, the CFD model is extended to include high crosswind states up to 50 m/s that cannot be readily or safely examined empirically. The results emphasize the critical role of the vent gas net heating value (NHV) on flare combustion and crosswind in reducing the CE. The comparison helps pave the way for further use of CFD simulation to improve flare designs and modes of operation and supports the use of parametric models to track and report methane losses from flaring.

Discovery of deep-sea acoels from a chemosynthesis-based ecosystem.

Chemosynthesis-based ecosystems such as hydrothermal vents and hydrocarbon seeps harbour various endemic species, each uniquely adapted to the extreme conditions. While some species rely on obligatory relationships with bacterial symbionts for nutrient uptake, scavengers and predators also play important roles in food web dynamics in these ecosystems. Acoels, members of the phylum Xenacoelomorpha, are simple, worm-like invertebrates found in marine environments worldwide but are scarcely understood taxa. This study presents a novel genus and species of acoel from a deep-sea hydrocarbon seep off Hatsushima, Japan, Hoftherma hatsushimaensis gen. et sp. nov. Our multi-locus phylogenetic analysis revealed that the acoels are nested within Hofsteniidae, a family previously known exclusively from shallow waters. This finding suggests that at least two independent colonization events occurred in the chemosynthesis-based environments from the phylum Xenoacoelomorpha, represented by hofsteniid acoels and Xenoturbella. Previous reports of hofsteniid species from low-oxygen and sulfide-rich environments, including intertidal habitats with decomposing leaves, in addition to H. hatsushimaensis gen. et sp. nov. from a deep-sea hydrocarbon seep, imply a common ancestral adaptation to sulfide-rich ecosystems within Hofsteniidae. Moreover, the sister relationship between solenofilomorphid acoels predominating in sulfide-rich habitats indicates common ancestral adaptation to sulfide-rich ecosystems between these two families.

Thermal dynamics in deep shale reservoirs: Influences of the kerogen microstructural behavior on the gas adsorption/desorption capacity

In shale gas extraction projects, an investigation into the mechanisms of energy/mass transfer associated with shale gas adsorption/desorption in organic matter (kerogen) microstructure under high temperature and stress condition is crucial for improving the efficiency of shale gas production. This study presents a coupling thermo-hydro-mechanical model based on an improved fractal method that could explain the microstructural evolution of the kerogen system and the resultant alterations during the gas adsorption/desorption process under varying thermal conduction, gas seepage, and stress conditions. The influence of porosity, diameter, and tortuosity on the abundance, length, and complexity of kerogen networks under coupled multi-field effects is evaluated. The significance of this study is it could address the following aspects quantitively: (1) the spatiotemporal evolution of kerogen fractal dimensions following various extraction timelines; (2) the influence of shale temperatures on kerogen structures; (3) the influence of the kerogen fractal dimension on the shale gas desorption capacity and production efficiency; and (4) under different temperatures, when the fractal dimension/tortuosity fractal dimension of kerogen changes due to extraction disturbances, the volumetric deformation induced by gas adsorption increases by a maximum of 26.1%/decreases by 28.1% and in the later stages of extraction, the maximum gas pressure decreases by 44.7%/increases by 47.1%. The proposed fractal method adeptly reveals shale gas desorption behaviors under multi-field coupling conditions from a microscopic perspective, which cannot be found in the literature.

Investigation of the Extent of Hydrothermal Activities of the Sikidang Crater, Dieng Volcanic Complex (Central Java), Using Geomagnetic Method

Abstract The Sikidang Crater (± 2000 m asl) is one of the active craters in the Dieng Volcanic Complex (DVC). It is situated on the southeastern flank of Mt. Pangonan. The crater is significant for development of both tourism and energy sectors in Dieng. Thermal manifestations inside the crater which consist of boiling mud pools and pots, hot springs, fumaroles, gas vents, steaming and altered ground make the place a popular tourist attraction. They also serve as windows to the shallow subsurface processes and help better understand the dynamics of the Dieng geothermal system. However, the land use of the crater and its vicinity disregards the fact that the actual extent of the hydrothermal activity of the crater has not been figured out, and that the foci of the thermal fluid discharges in the crater often shift. Some agriculture plots, housing areas, as well as tourism and geothermal infrastructures are located within or near the morphological boundary of the crater. The inherent hydrothermal hazard of the crater therefore, must be well mitigated to minimize the negative impacts to the people, as well as their assets. Our research aims to delineate the lateral and vertical extent of the hydrothermal activity of the crater by geomagnetic method. Geomagnetic anomaly in geothermal field occurs due to the difference in the intensity of hydrothermal alteration of the rocks. The equipment used was a pair of Proton Precision Magnetometers GEOTRON G5. Our result shows that low geomagnetic anomaly (-2200 to -400 nT) correlated with demagnetized, hydrothermally altered andesitic rocks covers an area of ≥ 1.15 km2, i.e., more than seven times larger than the area outlined by morphological boundary of the crater. The anomalous zone extends N/NE - S/SW from beyond the areas of inter-volcanic plain between Pangonan and Igir Binem in the north, to the areas beyond Sikunang and Kendil cones in the south. Below the Sikidang mud pool the demagnetized rock mass reaches a maximum thickness of 2000 m. The supporting data, i.e., the fluctuations of the gas discharge, and the seismicity of the DVC indicate that the recent dynamics of the Sikidang Crater is mostly controlled by the internal processes within the crater.

Carbonate chimneys at the highly productive point Dume methane seep: Fine-scale mineralogical, geochemical, and microbiological heterogeneity reflects dynamic and long-lived methane-metabolizing habitats.

Methane is a potent greenhouse gas that enters the marine system in large quantities at seafloor methane seeps. At a newly discovered seep site off the coast of Point Dume, CA, ~ meter-scale carbonate chimneys host microbial communities that exhibit the highest methane-oxidizing potential recorded to date. Here, we provide a detailed assessment of chimney geobiology through correlative mineralogical, geochemical, and microbiological studies of seven chimney samples in order to clarify the longevity and heterogeneity of these highly productive systems. U-Th dating indicated that a methane-driven carbonate precipitating system at Point Dume has existed for ~20 Kyr, while millimeter-scale variations in carbon and calcium isotopic values, elemental abundances, and carbonate polymorphs revealed changes in carbon source, precipitation rates, and diagenetic processes throughout the chimneys' lifespan. Microbial community analyses revealed diverse modern communities with prominent anaerobic methanotrophs, sulfate-reducing bacteria, and Anaerolineaceae; communities were more similar within a given chimney wall transect than in similar horizons of distinct structures. The chimneys represent long-lived repositories of methane-oxidizing communities and provide a window into how carbon can be transformed, sequestered, and altered over millennia at the Point Dume methane seep.

Carbon budget of methane seepages in the Haiyang 4 Area in the northern slope of the South China sea

Methane seepage records information of the local carbon cycle with respect to the generation, consumption and sequestration of carbon. Here presents the investigation of 7 gravity cores retrieved in 2004 during cruise SO-177 in the Haiyang 4 Area at the northern slope of the South China Sea. Porewater solutes, sulfate, methane, total alkalinity, sulfide and calcium demonstrate currently the weak seep activity. Local carbon cycling and sequestration is also revealed, that dominates by anaerobic oxidation of biogenic methane to dissolved bicarbonate inducing calcium carbonate and iron sulfide minerals (mainly pyrite) precipitation. A reactive transport model was employed to quantify the carbon cycle and budget. Model results show that current methane fluxes contribute to less than 2 wt % of authigenic carbonates and 2 wt % iron sulfide minerals being precipitated in 600–800 cm sediment depth. The sequestration of carbon could be > 50 mmol C cm−2 yr−1 under in situ condition. The observed increase of carbonate and iron sulfide minerals at ∼100 cm, however, require higher methane fluxes to shift the zone of anaerobic oxidation of methane upwards to around 1 m below the seafloor, which have occurred during sea level low stands in the geological past. The oscillation of seepage flux contributed to the formation of multiple layers of authigenic carbonates and pyrite, which indicates the high capability of carbon sink and is speculated to be induced by the dissociation of the underlying hydrates triggered by sea level drop and or temperature increase.

Extensive glacitectonism and rapid deglacial fluid expulsion in the northeastern Barents Sea

High-latitude regions store large amounts of carbon trapped by ice sheets, permafrost and gas hydrates, yet joint evolution of these climate components is poorly understood. Covered by a cold-based ice-sheet during the last glaciation, the seafloor of the northeastern Barents Sea reveals one of the largest known glacitectonic provinces in the Arctic. Up to 200-m high glacitectonic landforms are cratered and overlie faults associated with ‘bright spots’ indicative of subsurface gas accumulations. However, sediment samples show low pore gas concentrations and no present-day gas seepage. We combine new observations and ice-sheet - hydrate modelling to propose that during the Late Weichselian, glacitectonism was caused by patchy substrate stiffening due to gradual growth of subglacial gas hydrates and permafrost. Ice decay led to rapid destabilisation and full drainage of shallow hydrate reservoirs and permafrost thaw, causing craterisation which was likely accompanied by large fluxes of carbon released into the water column. This study shows that these processes were more widespread across glaciated margins, also highlighting sensitivity and potential for abrupt changes of high-latitude carbon pools in response to complex interactions between the cryosphere, ocean, and solid earth.

Salinity and nutrient condition as key factors drive the assembly of sediment prokaryotic communities

Despite extensive research on the geographical patterns of microbial communities, our comprehension of the mechanisms underlying their spatial distribution is still limited. Natural ecosystems provide opportunities to investigate the structure, connectivity, and assembly processes of prokaryotic communities. Saline lakes, mangroves, ocean margins, cold seeps, and open oceans as five distinct natural ecosystems exhibit varied levels of salinity and nutrient condition (carbon sources, electron donors, and electron acceptors). Based on the analysis of 197 sets of published 16S rRNA gene amplicon sequencing data on sediment samples of the five habitats, differences in salinity and nutrient conditions were identified to play a critical role in governing the composition, connectivity, and assembly process of prokaryotic communities. Specifically, unique prokaryotic community patterns were observed in these habitats, e.g., mangrove sediment communities were shown to have the highest alpha diversity and the lowest community-level ribosomal RNA gene operon (rrn) copy numbers, compared to the open ocean sediment communities. Positive correlation predominated connections (>80% of total connections) of the prokaryotic microbial networks in the five habitats. Communities within nutrient-rich saline lake and cold seep sediments exhibit the strongest and closest connections. Using the dissimilarity-overlap curve and null model, differences in composition, connectivity, and assembly process were found to be predominantly governed by deterministic forces. These findings enhance our understanding of microbial ecology in typical saline environments and enable us to investigate intricate ecosystems.

Zero-Net Liquid Flow Simulation Experiment and Flow Law in Casing Annulus Gas-Venting Wells

Under casing annulus gas venting, the annulus of the well is in a special state of zero-net liquid flow (ZNLF), leading to gas production without liquid at the wellhead, resulting in significant holdup issues. Therefore, conventional two-phase flow models cannot be used for calculation. To study the flow characteristics of ZNLF in the annulus of the well, this study established a visual experimental device with a total height of 5.4 m, an outer pipe inner diameter of 140 mm, and an inner pipe outer diameter of 72 mm. The flow characteristics of ZNLF were studied by controlling the casing pressure, initial liquid level, and bottom gas injection rate. The experimental results showed that the flow patterns of ZNLF are mainly bubbly flow and churn flow. Bubbly flow occurred at lower gas rates, while churn flow occurred at higher gas rates. In addition, the experiment found that when the gas injection rate and initial liquid column height were controlled to be the same, the liquid holdup decreased as the casing pressure increased. Analysis of the data patterns indicated that the slip velocity is related to the casing pressure. Based on the experimental results of ZNLF in the annulus, this study established standards for flow pattern transitions, holdup, and a pressure drop calculation model. The model results showed good agreement with the experimental results, with errors not exceeding ±5%.

SEM/Raman spectroscopy of clathrites as analogs of authigenic carbonates in ocean worlds

AbstractThere is evidence from the near‐infrared observations of space missions of the presence of carbonates on the surface of several ocean worlds. However, their genesis remains unresolved. We investigate the hypothesis that these carbonates may be in the form of clathrites assuming that clathrate hydrates are stable phases in the crust and ocean of these ocean worlds. In order to support this, we studied a sample of a potential clathrite from the Hydrate Ridge cold seep (Cascadia Subduction Zone), the carbonate rock fossil of clathrate hydrates, as a terrestrial analogue. We characterised the mineralogy and texture of the sample by using a coupled confocal Raman microscope and scanning electron microscopy instrument with the aim of identifying possible geo‐ and biosignatures, which could be relevant for future missions of exploration to ocean worlds and Mars. Our results show that aragonite is the dominant mineral phase in the clathrite sample, but Mg‐calcite and dolomite were also identified. These three carbonates constitute a pattern related to clathrate hydrate formation and dissociation processes. Dolomite was defined as a biosignature of gas hydrate microbiomes because it was integrated within Mg‐calcite grains precipitated after clathrate hydrate dissociation. Nevertheless, no spectral changes were observed in Raman bands of carbonate minerals that would indicate the influence of clathrate hydrates in their genesis. We also observed that Raman band positions of the associated framboidal pyrites are a characteristic signature of the associated framboid‐like texture because its potential as biosignature may only be attributed by biochemical analysis.

Metagenomic insights into Heimdallarchaeia clades from the deep-sea cold seep and hydrothermal vent

Heimdallarchaeia is a class of the Asgardarchaeota, are the most probable candidates for the archaeal protoeukaryote ancestor that have been identified to date. However, little is known about their life habits regardless of their ubiquitous distribution in diverse habitats, which is especially true for Heimdallarchaeia from deep-sea environments. In this study, we obtained 13 metagenome-assembled genomes (MAGs) of Heimdallarchaeia from the deep-sea cold seep and hydrothermal vent. These MAGs belonged to orders o_Heimdallarchaeales and o_JABLTI01, and most of them (9 MAGs) come from the family f_Heimdallarchaeaceae according to genome taxonomy database (GTDB). These are enriched for common eukaryote-specific signatures. Our results show that these Heimdallarchaeia have the metabolic potential to reduce sulfate (assimilatory) and nitrate (dissimilatory) to sulfide and ammonia, respectively, suggesting a previously unappreciated role in biogeochemical cycling. Furthermore, we find that they could perform both TCA and rTCA pathways coupled with pyruvate metabolism for energy conservation, fix CO2 and generate organic compounds through an atypical Wood-Ljungdahl pathway. In addition, many genes closely associated with bacteriochlorophyll and carotenoid biosynthesis, and oxygen-dependent metabolic pathways are identified in these Heimdallarchaeia MAGs, suggesting a potential light-utilization by pigments and microoxic lifestyle. Taken together, our results indicate that Heimdallarchaeia possess a mixotrophic lifestyle, which may give them more flexibility to adapt to the harsh deep-sea conditions.

Integrative taxonomy of a new giant deep-sea caudofoveate from South China Sea cold seeps

Caudofoveata is a class of worm-like molluscs (aplacophorans) that typically have an infaunal lifestyle, burrowing in soft bottoms in a wide range of marine habitats from shallow to deep waters. Here, we describe a very large new species of caudofoveate from South China Sea methane seeps growing up to 154 mm in length: Chaetoderma shenloongsp. nov. It is the first caudofoveate to be named from a chemosynthetic ecosystem and the first aplacophoran mollusc associated with seeps. Our new species stands out from other Pacific Chaetoderma species by its large size, a wide body relative to its length, a barely sclerotised radula, and the presence of isosceles-triangular sclerites. Phylogenetic reconstruction using the mitochondrial cytochrome c oxidase subunit I (COI) gene placed it within a paraphyletic clade comprising Chaetodermatidae and Limifossoridae, in line with a previous phylogenetic analysis. This also revealed that C. shenloongsp. nov. is conspecific with a Chaetoderma sp. whose whole genome was recently sequenced and assembled but remained undescribed until now. The most closely related species with an available COI sequence was C. felderi, the largest caudofoveate species recorded. Our discovery suggests caudofoveates may be present in other seeps globally but so far neglected; a potential example is C. felderi from the Gulf of Mexico, where seeps are abundant but whose exact habitat remains unclear.

Analysis of Hydraulic Permeability Enhancement Effect Based on an Experimental System of Highly Pressurized Water Invasion and Gas Seepage

Analysis of Hydraulic Permeability Enhancement Effect Based on an Experimental System of Highly Pressurized Water Invasion and Gas Seepage

Hydrocarbon‐derived thrombolites from the Outer Carpathians (Lower Cretaceous, Poland)

AbstractShallow marine thrombolites from a newly discovered Lower Cretaceous cold seep in the Outer Carpathians (Poland) were analysed in order to untangle the complex sedimentological and biogeochemical processes involved in their formation and their diagenetic modifications. The studied thrombolites are made of two components: (i) microcrystalline mesoclots; and (ii) spar‐filled framework cavities. These components are dominated by calcite and show a complex spatial relationship, resulting in a heterogeneous, clotted fabric. The mesoclots exhibit digitate structures, often concentrically‐laminated, and are chiefly composed of microcrystalline material with δ13C values from −34.8 to −19.4‰ PeeDee Belemnite. Biomarkers analyses show the presence of 2, 6, 10, 15, 19‐pentamethylicosane within the mesoclots. The mesoclots host <5 mm wide microtubes filled with isopachous calcite recording even lower δ13C values (from −39.0 to −20.5‰ PeeDee Belemnite). The morphology of the mesoclots relative to their internal lamination and their geochemistry indicates that their growth was linked to anaerobic oxidation of methane while the microtubes acted as conduits for hydrocarbon‐charged fluids. The framework cavities are internally lined with framboidal pyrite, and are cemented by calcite spar with relatively high δ13C (−15.1 to −7.3‰ PeeDee Belemnite) and low δ18O values (−9.3 to −4.4‰ PeeDee Belemnite). Carbonate precipitation within the framework cavities is interpreted to have been related to bacterial sulphate reduction. U‐shaped trace fossils attributed to the ichnogenus Balanoglossites cross‐cut both mesoclots and framework cavities. The mechanisms involved in the formation and diagenesis of thrombolites at cold seeps are yet to be fully understood, and this work provides new insights on these complex biogeochemical and sedimentological processes.

A Modified Model of Micro-Scale Gas Seepage in Rock Porous Media

It is of significance to study the micro-scale gas seepage mechanism in rock porous media due to shale gas exploitation and prevention of tunnel gas explosion. However, current microscale seepage models have problems, such as poor accuracy or small computational domain. In this work, a representative volume element scale microscopic seepage model with high precision and large computational domain is established using the lattice Boltzamnn method. In the representative volume element scale model, micro-scale effect is considered by the Beskok-Karniadakis correction relationship. Then the accuracy of the representative volume element scale model is improved by proposing an equivalent resistance correction coefficient. The correction coefficient value is obtained by comparing the calculated result for the flow rate at a representative volume element scale and more precise pore scale. For example, when the porosity is 0.9 and the dimensionless specific surface area is 30, the corrected model can reduce the average deviation from 34.38 to 0.14%. Finally, to expand the applicability of the proposed model, a correlation for the correction coefficient is introduced as a function of the Knudsen number, porosity of porous media, and dimensionless specific surface area. The mean absolute percentage deviation of the correction coefficient correlation is 9.25%.