Soil Methane Research Articles

Coupling Stable Isotope Analysis with Gas Push‐Pull Tests to Derive In Situ Values for the Fractionation Factor αox Associated with the Microbial Oxidation of Methane in Soils

Core Ideas: Methane CH4 isotopologue fraction was measured in situ for the first time. αox varied strongly with oxidation rate. αox varied between landfills and between individual points on the same landfill. αox varied with spatial and temporal variability of soil properties. Our findings limit application of stable isotopes for CH4 oxidation quantification. A prerequisite for the application of stable isotope fractionation for the quantification of the methane (CH4) oxidation efficiency of landfill covers is that the fractionation factor αox is known or can be estimated with adequate accuracy. So far, αox has only been determined in laboratory experiments. In this study, αox was determined under in situ conditions in the field by coupling two independent methods, gas push‐pull tests and stable isotope analysis, to assess biological fractionation of CH4 isotopologues in landfill cover soils. On six landfills with nine points of investigation, 22 measurements were performed, covering a wide range of environmental conditions, such as soil temperature and moisture and observed oxidation rates. Values for αox varied between near 1, indicating little fractionation, and 1.151. Correlation of αox with the CH4 oxidation rate found by gas push‐pull tests revealed a clear asymptotic relationship, with low rates being associated with high values for αox and high rates resulting in little fractionation. Values for αox varied between the different landfills and between the individual points of investigation on the same landfill. The latter is assumed to reflect the spatial variability of methanotrophic activity due to spatial variability in soil moisture and hence air‐filled porosity as well as the spatial variability of gas fluxes. Significant variation of αox was observed for the same sampling point, presumably reflecting the temporal variability of factors influencing methanotrophic activity. These effects could include seasonally changing environmental conditions (e.g., soil temperature and moisture) but also the temporal variability of gas fluxes through the landfill soil cover, changing exposure of methanotrophs to CH4 and oxygen and hence their activity. The quantification of the CH4 oxidation efficiency using fractionation of stable isotopes is very sensitive to αox. Assuming a value constant in time and space and transferring this value from laboratory experiments to field settings entails significant uncertainty regarding the quantification of CH4 oxidation.

Short-term effects of biochar and salinity on soil greenhouse gas emissions from a semi-arid Australian soil after re-wetting

Arid and semi-arid soils often show a pulse of soil greenhouse gas (GHG) emissions upon re-wetting – whether from irrigation water or rainfall. We used a laboratory incubation to elucidate interactions of salinity, biochar amendment, and simulated wetting intensity in emissions of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in a semi-arid Australian soil. A factorial experimental design was used with three main factors: irrigation water salinity (using NaCl, control or ~0.9dSm−1, 5dSm−1 and 10dSm−1), biochar amendment (0% and 5% by mass of Eucalyputs polybractea biochar) and soil moisture (25%, 50%, 75% and 100% of water-holding capacity, WHC – a proxy for wetting intensity after irrigation or rainfall). The strongest single regulating variable of rates of soil CO2 emission was WHC (+171% increase between 25% and 100% WHC). Salinity reduced CO2 emissions (relative to controls) by −19% at 5dSm−1 and −28% at 10dSm−1. Soils amended with biochar produced less (−10%) CO2 emissions. All treatments showed negative CH4 emissions (or CH4 oxidation) that were only influenced by WHC. Soil N2O emissions increased with salinity (+60%), while biochar additions reduced them slightly (−12%). N2O emissions were not influenced by WHC. Overall, results showed that biochar additions can mitigate some of the “pulse” effects of rainfall on emissions (~10% in term of global warming potential across all treatments).

2D profiles of CO2, CH4, N2O and gas diffusivity in a well aerated soil: measurement and Finite Element Modeling

Soil gas fluxes depend on soil gas concentrations and physical properties of a soil. Taking soil samples for physical analysis into the laboratory strongly modifies soil gas concentrations and also cuts roots that sustain the activity in the rhizosphere. Since microbial processes interact with gas concentrations in soil, we need to study gas transport and production in situ.We developed a method to monitor the transport and production and consumption of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in soils in situ in a two dimensional (2D) profile using tetrafluoromethane (CF4) and sulfur hexafluoride (SF6) as tracer gases and Finite Element Modeling of soil gas transport. Continuous injection of the inert tracer gases and 2D gas sampling in a soil profile allowed for inverse modeling of the 2D profile of soil gas diffusivity. In a second step, the 2D profiles of the production and consumption of CO2, CH4, and N2O were inversely determined.Soil gas concentrations were monitored in a Scots pine stand in South-West Germany during a rain-free week in the fall. The 2D relative (so as to be independent of gas species) soil gas diffusivity profile showed large horizontal variability. Relative soil gas diffusivity was found to be anisotropic with the vertical direction greater by a factor of 1.26. Topsoil moisture decreased slowly over time resulting in an increase in relative soil gas diffusivity. The soil was found to be a source of CO2, and a net sink of CH4 and N2O, with the highest production (CO2) and consumption (CH4, N2O) occurring in the topsoil. The gas concentration and production profiles of CO2 were nearly horizontally homogenous, while those for CH4 showed larger horizontal differences. Net consumption of CH4 and net production of CO2 both increased as the soil dried. This occurred despite reverse trends for these variables in the topsoil (0–8cm depth) which were more than offset by the underlying soil becoming more active. Sensitivity tests showed that the determination of 2D profiles of soil gas diffusivity and production and consumption of CO2 and CH4 were more reliable than the estimates for N2O because the magnitudes of these for N2O were very low. Our method represents a useful tool for the analyses of soil gas flux heterogeneities and associated microbial processes within soil profiles.

Methane Emission from Pineapple Cultivation on a Tropical Peatland at Saratok, Malaysia

Information on methane emission in pineapple cultivation on peatlands is scarce. Methane emission in pineapple cultivation is important as 90% of pineapples are grown on the peat soils of Malaysia. It is essential to determine methane emission in pineapple cultivation because pineapples are Crassulacean acid metabolism plants whose effects on methane could be different from other crops grown on tropical peat soils. Methane emissions from root respiration, microbial respiration, and oxidative peat decomposition were determined in a lysimeter experiment. There were three treatments: peat soil cultivated with pineapple, bare peat soil, and bare peat soil fumigated with chloroform. Methane emissions from peat soil cultivated with pineapple, bare peat soil, and bare peat soil fumigated with chloroform were 0.65 t/ha/yr, 0.75 t/ha/yr, and 0.75 t/ha/yr, respectively. The lower methane emissions are consistent with the general believe that methane emission from cultivated peat soils is lower than those of anaerobic or water logged peat soils. Soil methane emission was affected by nitrogen fertilization under pineapple cultivation but the converse was true for soil temperature nor soil moisture.

Cryogenic Displacement and Accumulation of Biogenic Methane in Frozen Soils

Evidences of highly localized methane fluxes are reported from the Arctic shelf, hot spots of methane emissions in thermokarst lakes, and are believed to evolve to features like Yamal crater on land. The origin of large methane outbursts is problematic. Here we show, that the biogenic methane (13C ≤ −71‰) which formed before and during soil freezing is presently held in the permafrost. Field and experimental observations show that methane tends to accumulate at the permafrost table or in the coarse-grained lithological pockets surrounded by the sediments less-permeable for gas. Our field observations, radiocarbon dating, laboratory tests and theory all suggest that depending on the soil structure and freezing dynamics, this methane may have been displaced downwards tens of meters during freezing and has accumulated in the lithological pockets. The initial flux of methane from the one pocket disclosed by drilling was at a rate of more than 2.5 kg C(CH4) m−2 h−1. The age of the methane was 8–18 thousand years younger than the age of the sediments, suggesting that it was displaced tens of meters during freezing. The theoretical background provided the insight on the cryogenic displacement of methane in support of the field and experimental data. Upon freezing of sediments, methane follows water migration and either dissipates in the freezing soils or concentrates at certain places controlled by the freezing rate, initial methane distribution and soil structure.

土壌におけるCH_4の放出および吸収の定量化( 陸上生態系における炭素フラックス調査法 現状と今後の課題)

土壌におけるCH_4の放出および吸収の定量化( 陸上生態系における炭素フラックス調査法 現状と今後の課題)

Emission and distribution of phosphine in paddy fields and its relationship with greenhouse gases

Phosphine (PH3), as a gaseous phosphide, plays an important role in the phosphorus cycle in ecosystems. In this study, the emission and distribution of phosphine, carbon dioxide (CO2) and methane (CH4) in paddy fields were investigated to speculate the future potential impacts of enhanced greenhouse effect on phosphorus cycle involved in phosphine by the method of Pearson correlation analysis and multiple linear regression analysis. During the whole period of rice growth, there was a significant positive correlation between CO2 emission flux and PH3 emission flux (r=0.592, p=0.026, n=14). Similarly, a significant positive correlation of emission flux was also observed between CH4 and PH3 (r=0.563, p=0.036, n=14). The linear regression relationship was determined as [PH3]flux=0.007[CO2]flux+0.063[CH4]flux−4.638. No significant differences were observed for all values of matrix-bound phosphine (MBP), soil carbon dioxide (SCO2), and soil methane (SCH4) in paddy soils. However, there was a significant positive correlation between MBP and SCO2 at heading, flowering and ripening stage. The correlation coefficients were 0.909, 0.890 and 0.827, respectively. In vertical distribution, MBP had the analogical variation trend with SCO2 and SCH4. Through Pearson correlation analysis and multiple stepwise linear regression analysis, pH, redox potential (Eh), total phosphorus (TP) and acid phosphatase (ACP) were identified as the principal factors affecting MBP levels, with correlative rankings of Eh>pH>TP>ACP. The multiple stepwise regression model ([MBP]=0.456∗[ACP]+0.235∗[TP]−1.458∗[Eh]−36.547∗[pH]+352.298) was obtained. The findings in this study hold great reference values to the global biogeochemical cycling of phosphorus in the future.

Soil microbial community structure and diversity are largely influenced by soil pH and nutrient quality in 78-year-old tree plantations

Abstract. Forest plantations have been recognised as a key strategy management tool for stocking carbon (C) in soils, thereby contributing to climate warming mitigation. However, long-term ecological consequences of anthropogenic forest plantations on the community structure and diversity of soil microorganisms and the underlying mechanisms in determining these patterns are poorly understood. In this study, we selected 78-year-old tree plantations that included three coniferous tree species (i.e. slash pine, hoop pine and kauri pine) and a eucalypt species in subtropical Australia. We investigated the patterns of community structure, and the diversity of soil bacteria and eukaryotes by using high-throughput sequencing of 16S rRNA and 18S rRNA genes. We also measured the potential methane oxidation capacity under different tree species. The results showed that slash pine and Eucalyptus significantly increased the dominant taxa of bacterial Acidobacteria and the dominant taxa of eukaryotic Ascomycota, and formed clusters of soil bacterial and eukaryotic communities, which were clearly different from the clusters under hoop pine and kauri pine. Soil pH and nutrient quality indicators such as C : nitrogen (N) and extractable organic C : extractable organic N were key factors in determining the patterns of soil bacterial and eukaryotic communities between the different tree species treatments. Slash pine and Eucalyptus had significantly lower soil bacterial and eukaryotic operational taxonomical unit numbers and lower diversity indices than kauri pine and hoop pine. A key factor limitation hypothesis was introduced, which gives a reasonable explanation for lower diversity indices under slash pine and Eucalyptus. In addition, slash pine and Eucalyptus had a higher soil methane oxidation capacity than the other tree species. These results suggest that significant changes in soil microbial communities may occur in response to chronic disturbance by tree plantations, and highlight the importance of soil pH and physiochemical characteristics in microbially mediated ecological processes in forested soils.

Difference in Soil Methane (CH4) and Nitrous Oxide (N2O) Fluxes from Bioenergy Crops SRC Willow and SRF Scots Pine Compared with Adjacent Arable and Fallow in a Temperate Climate

Soil greenhouse gas (GHG) fluxes of methane (CH4) and nitrous oxide (N2O) were measured over a 2-year period from several land use systems on adjacent sites under the same soil and climatic conditions to assess the influence of the transition from arable agricultural (barley) and fallow to perennial bioenergy crops short rotation coppice (SRC) willow (Salix spp.) and short rotation forest (SRF) Scots pine (Pinus sylvestris). There were no significant differences between CH4 and N2O fluxes measured from the SRC, SRF and fallow, but the arable agricultural site showed an order of magnitude higher N2O emissions compared with the others. Fertiliser application to the arable crop was the major factor influencing N2O emissions, and both air and soil temperature showed no significant effects on fluxes between the different land use systems. Soil moisture was significantly different from the arable crop, showing a greater range than from SRF and SRC. Hence, these bioenergy crops might be viable options for water-stressed areas.

Quantifying and predicting spatio-temporal variability of soil CH4 and N2O fluxes from a seemingly homogeneous Australian agricultural field

Soil methane (CH4) and nitrous oxide (N2O) fluxes are difficult to predict from soil temperature and moisture alone, especially compared to carbon dioxide (CO2) fluxes. That difficulty is reflected in high spatial and temporal (spatiotemporal) variability of these two greenhouse gases (GHGs). We used a 16ha field, under homogeneous soils and vegetation, to simultaneously explore spatial and temporal variability of soil CH4 and N2O fluxes. We also measured soil physical and chemical properties in order to explain, and predict, spatial variability of these two gases. Gas fluxes were measured using either a dynamic chamber (spatial variability study) or automated chambers using FTIR (temporal variability study). Soil samples were analysed for 30 chemical parameters (including at least two forms of soil carbon and nitrogen), while two proximal soil sensors were used to collect fine-resolution soil electrical conductivity and gamma radiometric concentration across the site. Fluxes of CH4 and N2O showed distinct spatial patterns, and were uniquely related to soil properties. Spatial variability in both CH4 and N2O fluxes was greater than five months of temporal variability (an increase in 112% and 39% in standard deviations for each gas respectively). If we relied solely on the autochambers for mean field fluxes, we would have underestimated fluxes by 59 and 197%, for CH4 and N2O respectively. CH4 fluxes were more spatially-dependent than those of N2O (semivariance analysis), but both showed greater spatial dependence than previously reported. Nearly 40 and 50% of the mean spatial flux of CH4 and N2O were from 1% of the area. Spatial variability in soil CH4 fluxes was predicted best by electrical conductivity measurements at 0–50cm (r=0.74) and soil C. Soil N2O fluxes, on the other hand, were predicted best by soil N and the gamma radiometric data (r=0.48). Overall, our results clearly show that the large spatial variance of both CH4 and N2O fluxes requires great caution when scaling from chamber-based measurements to the field and beyond. Proximal sensors (as used here) can help map “hot spots” of soil CH4 and N2O fluxes at the field scale.

Combining soil and tree‐stem flux measurements and soil gas profiles to understand CH4 pathways in Fagus sylvatica forests

AbstractQuantifying and understanding fluxes of methane (CH4) and carbon dioxide (CO2) in natural soil–plant–atmosphere systems are crucial to predict global climate change. Wetland herbaceous species or tree species at waterlogged sites are known to emit large amounts of CH4. Upland forest soils are regarded as CH4 sinks and tree species like upland beech are not known to significantly emit CH4. Yet, data are scarce and this assumption needs to be tested.We combined measurements of soil–atmosphere and stem–atmosphere fluxes of CO2 and CH4, and soil gas profiles to assess the contribution of the different ecosystem compartments at two upland beech forest sites in Central Europe in a case study. Soil was a net CH4 sink at both sites, though emissions were detected consistently from beech stems at one site. Although stem emissions from beech stems were high compared to known fluxes from other upland tree species, they were substantially lower compared to the strong CH4 sink of the soil. Yet, we observed extraordinarily large CH4 emissions from one beech tree that was 140% of the CH4 sink of the soil. The soil gas profile at this tree indicated CH4 production at a soil depth > 0.3 m, despite the net uptake of CH4 consistently observed at the soil surface. Field soil assessment showed strong redoximorphic color patterns in the adjacent soil and supports this evaluation. We hypothesize that there is a transport link between the soil and stem via the root system representing a preferential transport mechanism for CH4 despite the fact that beech roots usually do not bear aerenchyma. The high mobility of gases requires a holistic view on the soil–plant–atmosphere system. Therefore, we recommend including field soil assessment and soil gas profiles measurements when investigating soil–atmosphere and stem–atmosphere fluxes to better understand the sources of gases and their transport mechanisms.

Soil methane oxidation in both dry and wet temperate eucalypt forests shows a near-identical relationship with soil air-filled porosity

Abstract. Well-drained, aerated soils are important sinks for atmospheric methane (CH4) via the process of CH4 oxidation by methane-oxidising bacteria (MOB). This terrestrial CH4 sink may contribute towards climate change mitigation, but the impact of changing soil moisture and temperature regimes on CH4 uptake is not well understood in all ecosystems. Soils in temperate forest ecosystems are the greatest terrestrial CH4 sink globally. Under predicted climate change scenarios, temperate eucalypt forests in south-eastern Australia are predicted to experience rapid and extreme changes in rainfall patterns, temperatures and wild fires. To investigate the influence of environmental drivers on seasonal and inter-annual variation of soil–atmosphere CH4 exchange, we measured soil–atmosphere CH4 exchange at high-temporal resolution (< 2 h) in a dry temperate eucalypt forest in Victoria (Wombat State Forest, precipitation 870 mm yr−1) and in a wet temperature eucalypt forest in Tasmania (Warra Long-Term Ecological Research site, 1700 mm yr−1). Both forest soil systems were continuous CH4 sinks of −1.79 kg CH4 ha−1 yr−1 in Victoria and −3.83 kg CH4 ha−1 yr−1 in Tasmania. Soil CH4 uptake showed substantial temporal variation and was strongly controlled by soil moisture at both forest sites. Soil CH4 uptake increased when soil moisture decreased and this relationship explained up to 90 % of the temporal variability. Furthermore, the relationship between soil moisture and soil CH4 flux was near-identical at both forest sites when soil moisture was expressed as soil air-filled porosity (AFP). Soil temperature only had a minor influence on soil CH4 uptake. Soil nitrogen concentrations were generally low and fluctuations in nitrogen availability did not influence soil CH4 uptake at either forest site. Our data suggest that soil MOB activity in the two forests was similar and that differences in soil CH4 exchange between the two forests were related to differences in soil moisture and thereby soil gas diffusivity. The differences between forest sites and the variation in soil CH4 exchange over time could be explained by soil AFP as an indicator of soil moisture status.

Tree-based intercropping may reduce, while fertilizer nitrate may increase, soil methane emissions

Tree-based intercropping (TBI) systems have shown some promise in mitigating greenhouse gas emissions, such as by sequestering carbon and decreasing soil nitrous oxide emissions. However, the effects of TBI on soil methane fluxes remain unknown. In a field study, we failed to show differences in soil CH4 production between TBI and conventional monocropping (CM) systems. Within TBI plots, however, we found significantly lower CH4 concentrations near the middle of the alleys than closer to tree rows. Soil CH4 concentrations also decreased with soil depth, even dipping below mean global atmospheric concentrations. Laboratory assays revealed a higher CH4 oxidation potential in soils collected from TBI plots compared with CM plots. These assays also revealed a decrease in CH4 oxidation potential after soils were amended with nitrate. We conclude that TBI could potentially reduce soil CH4 emissions, whereas fertilizer nitrate may increase them.

Trapped Greenhouse Gases in the Permafrost Active Layer: Preliminary Results for Methane Peaks in Vertical Profiles of Frozen Alaskan Soil Cores

Degradation of organic carbon stored in permafrost may represent an additional source of atmospheric greenhouse gases (GHGs) in a warming climate. However, there is no clear understanding of how seasonal freeze–thaw affects gas permeability and emission of methane in permafrost soils, in part due to the lack of chemical and physical characterisation of the soils. Here, we report vertical profiles of CO2, CH4 and other soil properties with resolutions of 0.05–0.1 m depth from five soil cores, drilled to 0.9 m depth, in Alaskan permafrost during the early spring of 2013, when the ground was frozen under snow cover. Two cores from tundra and black spruce bog showed sudden increases in methane concentration (up to 416 µmol litre−1soil), indicating excess CH4 trapped in gas-impermeable soil layers during the freezing period. Active-layer cooling in late autumn, producing a frozen surface and relatively warm sub-surface, may have allowed underground microbial activity and facilitated CH4 production, while the upward transport of CH4 was hampered by the frost. Our preliminary results show that estimating annual GHG efflux from permafrost regions can be complicated because of heterogeneous distributions of trapped methane within soil columns. Copyright © 2016 John Wiley & Sons, Ltd.

Spatial micro-distribution of methanotrophic activity along a 120-year afforestation chronosequence

Methanotrophic bacteria drive upland soil methane (CH4) uptake. Land-use change often affects their activity, but the mechanisms involved are not well understood. We studied soil-atmosphere CH4 fluxes along a 120-year Norway spruce afforestation chronosequence on subalpine pasture, testing whether effects were related to shifts in the spatial niche of methanotrophs. Previous field data had shown that soil 14CH4 uptake increased with forest age, and that this effect was driven by decreased water filled pore space due to higher rainfall interception in the more developed canopies of older forest stands. The spatial distribution of methanotrophic activity was determined by 14CH4-labelling followed by soil section preparation, aggregate size fractionation, aggregate erosion, and micro-autoradiographic imaging. Uptake rates of CH4 measured in laboratory incubations of soil cores as well as their water contents largely followed the in situ measurements previously made in the field. 14CH4 assimilation was heterogeneously distributed, and occurred further down the soil profile in older forest that had a more developed organic layer that did not contribute to CH4 uptake. Assimilation was largest in 2—8 mm aggregates, and higher at the exterior than in the interior of aggregates. Our data indicates that differences in soil aggregation and related methanotrophic activities did not contribute substantially to higher CH4 uptake in older forest, mostly because aggregation did not change much with age. On a per mass basis, however, large aggregates contributed less to CH4 uptake due to their unfavorable surface to volume ratio. More generally, we argue that the (sub-)aggregate heterogeneity of soil microbial activity and diversity is underexplored, although it critically determines ecological interactions that drive ecosystem-level processes.

Effects of nitrogen and phosphorus additions on soil methane uptake in disturbed forests

AbstractAtmospheric nitrogen (N) deposition is generally thought to suppress soil methane (CH4) uptake in natural forests, and phosphorus (P) input may alleviate this negative effect. However, it remains unclear how N and P inputs control soil CH4 uptake in disturbed forests. In this study, soil CH4 uptake rates were measured in two disturbed forests, including a secondary forest (with previous, but not recent, disturbance) and a plantation forest (with recent continuous disturbance), in southern China for 34 months of N and/or P additions: control, N addition (150 kg N ha−1 yr−1), P addition (150 kg P ha−1 yr−1), and NP addition (150 kg N ha−1 yr−1 plus 150 kg P ha−1 yr−1). Mean CH4 uptake rate in control plots was significantly higher in the secondary forest (24.40 ± 0.81 µg CH4‐C m−2 h−1) than in the plantation forest (17.07 ± 0.70 µg CH4‐C m−2 h−1). CH4 uptake rate had negative relationships with soil water‐filled pore space in both forests. In the secondary forest, N, P, and NP additions significantly decreased CH4 uptake by 39.7%, 27.8%, and 37.6%, respectively, but had no significant effects in the plantation forest, indicating that P input does not alleviate the suppression of CH4 uptake by N deposition. Taken together, our findings suggest that reducing anthropogenic disturbance, including harvesting of forest floor, and anthropogenic N and P inputs will increase soil CH4 uptake in disturbed forests, which is important in view of the increased trends in global warming during recent decades.

Effects of nitrogen and biochar amendment on soil methane concentration profiles and diffusion in a rice-wheat annual rotation system.

The CH4 emissions from soil were influenced by the changeable CH4 concentrations and diffusions in soil profiles, but that have been subjected to nitrogen (N) and biochar amendment over seasonal and annual time frames. Accordingly, a two-year field experiment was conducted in southeastern China to determine the amendment effects on CH4 concentrations and diffusive effluxes as measured by a multilevel sampling probe in paddy soil during two cycles of rice-wheat rotations. The results showed that the top 7-cm soil layers were the primary CH4 production sites during the rice-growing seasons. This layer acted as the source of CH4 generation and diffusion, and the deeper soil layers and the wheat season soil acted as the sink. N fertilization significantly increased the CH4 concentration and diffusive effluxes in the top 7-cm layers during the 2013 and 2014 rice seasons. Following biochar amendment, the soil CH4 concentrations significantly decreased during the rice season in 2014, relative to the single N treatment. Moreover, 40 t ha−1 biochar significantly decreased the diffusive effluxes during the rice seasons in both years. Therefore, our results showed that biochar amendment is a good strategy for reducing the soil profile CH4 concentrations and diffusive effluxes induced by N in paddy fields.

Wetland invasion by Typha × glauca increases soil methane emissions

Wetland invasion by monotypic dominant plants can alter the physicochemical and biological properties of soils that affect methane emissions, a potent greenhouse gas. We examined the effects of Typha×glauca invasion on soil methane using laboratory incubation and controlled mesocosm experiments. Typha-invaded soils collected from three Midwestern (USA) wetlands had greater methane production potential during laboratory incubation than soils dominated by native wet meadow vegetation. Ten years post-invasion of native plant-dominated mesocosms, Typha increased methane emissions at least three-fold (native: 15.0±10.5mg CH4-Cm−2 h−1, median: 6.1mg CH4-Cm−2h−1; Typha: mean: 45.9±16.7mg CH4-Cm−2h−1, median: 26.8mg CH4-Cm−2h−1) under high (+10cm) water levels, though methane emissions were negligible under low (–10cm) water levels. Methane emissions were positively correlated with soil carbon, nitrogen, and aboveground biomass, all of which were greater in Typha-invaded mesocosms. Together, our data suggest that replacement of large tracts of native wetlands throughout eastern North America with monocultures of invasive Typha could alter regional methane emissions.

Redox dynamics in the active layer of an Arctic headwater catchment; examining the potential for transfer of dissolved methane from soils to stream water

AbstractThe linkages between methane production, transport, and release from terrestrial and aquatic systems are not well understood, complicating the task of predicting methane emissions. We present novel data examining the potential for the saturated zone of active layer soils to act as a source of dissolved methane to the aquatic system, via soil water discharge, within a headwater catchment of the continuous permafrost zone in Northern Canada. We monitored redox conditions and soil methane concentrations across a transect of soil profiles from midstream to hillslope and compare temporal patterns in methane concentrations in soils to those in the stream. We show that redox conditions in active layer soils become more negative as the thaw season progresses, providing conditions suitable for net methanogenesis and that redox conditions are sensitive to increased precipitation during a storm event—but only in shallower surface soil layers. While we demonstrate that methane concentrations at depth in the hillslope soils increase over the course of the growing season as reducing conditions develop, we find no evidence that this has an influence on stream water methane concentrations. Sediments directly beneath the stream bed, however, remain strongly reducing at depth throughout the thaw season and contain methane at concentrations 5 orders of magnitude greater than those in hillslope soils. The extent of substreambed methane sources, and the rates of methane transport from these zones, may therefore be important factors determining headwater stream methane concentrations under changing Arctic hydrologic regimes.

A five-year study of the impact of nitrogen addition on methane uptake in alpine grassland.

It remains unclear how nitrogen (N) deposition affects soil methane (CH4) uptake in semiarid and arid zones. An in situ field experiment was conducted from 2010 to 2014 to systematically study the effect of various N application rates (0, 10, 30, and 90 kg N ha−1 yr−1) on CH4 flux in alpine grassland in the Tianshan Mountains. No significant influence of N addition on CH4 uptake was found. Initially the CH4 uptake rate increased with increasing N application rate by up to 11.5% in 2011 and then there was gradual inhibition by 2014. However, the between-year variability in CH4 uptake was very highly significant with average uptake ranging from 52.9 to 106.6 μg C m−2 h−1 and the rate depended largely on seasonal variability in precipitation and temperature. CH4 uptake was positively correlated with soil temperature, air temperature and to a lesser extent with precipitation, and was negatively correlated with soil moisture and NO3−-N content. The results indicate that between-year variability in CH4 uptake was impacted by precipitation and temperature and was not sensitive to elevated N deposition in alpine grassland.