Excessive water hyacinth growth in aquatic environments and metanil yellow (MY) dye in industrial wastewater pose severe environmental and public health challenges. Therefore, this study evaluated the effects of various process factors on batch MY biosorption onto water hyacinth leaves (LECs) and MY biosorption kinetics, equilibrium, and thermodynamics. The optimal pH for MY biosorption by LECs was 1.5-2.0. The initial MY concentration affected the equilibrium MY biosorption capacity but not the LEC particle size and solution temperature. However, the LEC particle size and solution temperature affected the MY biosorption rate; the biosorption rate was higher at a lower particle size (0.15-0.3 mm) and a higher temperature (62 °C) than at higher particle sizes and lower temperatures. The pseudo-second-order model adequately described the biosorption kinetics of MY by LECs at the different levels of the process factors, whereas the Sips and Redlich-Peterson models satisfactorily represented the biosorption isotherm of MY. The Sips model predicted a maximum MY biosorption capacity of 170.8 mg g-1. The biosorption of MY by LECs was endothermic and not spontaneous. These findings demonstrate that LECs exhibit great potential for bioremediating MY-contaminated wastewater, thereby providing valuable insights for effective water treatment and pollution control strategies.