Metals In Different Organs Research Articles

A Study on Bioaccumulation of Heavy Metals in two Anuran Tadpoles: Clinotarsus alticola and Leptobrachium smithi from Rosekandy Tea Estate, Cachar, Assam

Considering the significance of heavy metal pollution in aquatic system bioaccumulation of heavy metals in two species of tadpoles namely Clinotarsus alticola and Leptobrachium smithicollected from tea gardens of Barak valley, Assam was studied. Aquatic life is affected by heavy metal pollutants present in water as well as in sediment. The result of the study revealed that the concentration of iron, chromium, cadmium and lead in water samples was higher than the permissible limit of 0.3, 0.05, 0.003, 0.01 mg/L respectively but that of copper and zinc concentration was within the maximum permissible limit of 2 mg/L and 3 mg/L (WHO, 2005). The accumulation pattern of different heavy metals in different organs viz., intestine, liver and tail was studied.Overall the metal burden in different organs of Clinotarsus alticola and Leptobrachium smithi was in the order of liver>tail>intestine. Liver had highest accumulation of metals while intestine accumulated the least.Iron (Fe) was highly and zinc (Zn) was the least accumulated metal in both the tadpoles. The accumulation of heavy metals might be due to tea plantation influx water, domestic and associated anthropogenic activities.

Effects of Some Heavy Metals in Different Organs and Some Hepatic Enzymes for European eel (Anguilla anguilla) at Lake Edku

Effects of Some Heavy Metals in Different Organs and Some Hepatic Enzymes for European eel (Anguilla anguilla) at Lake Edku

Effect of Heavy Metals on Liver, Kidney, Gills and Muscles of Cyprinus carpio and Wallago attu inhabited in the Indus

The present study was aimed to evaluate the effect of heavy metals on an important tissue of two fish species Cyprinus carpio and Wallago attu, sampled from the Indus river, Mianwali District, Pakistan. The concentration of selected heavy metals Fe, Cr, Cu, and in gills, muscles, kidney and liver was compared with an International standard of food fish. The overall metal concentrations among different weight categories in C. carpio were in the order of Fe > Cu > Cr >. In W. attu the overall accumulation of these metals were, in order of Fe > Cu > Cr > Pb The order of accumulation of metals in gills and muscle of C. carpio was Fe > Cr > Pb > Cu; kidney and muscles of W. attu was Fe > Cr > Cu > Pb; liver Fe > Cu > Cr > Pb. An increasing trend of concentration of iron, copper, chromium and lead occurred with an increase in weight of C. carpio and W. attu. There was a significant difference in the accumulation of heavy metals in different organs of both species (p<0.01). All studied heavy metals except Cr were within permissible limits described by various international agencies like WHO, FAO and FEPA in edible tissues of C. carpio and W. attu.

Bioaccumulation of Heavy Metals in Different Organs of Wallago attu from River Kabul Khyber Pakhtunkhwa, Pakistan.

The aim of the study was to quantify the accumulation of heavy metals like Zn, Ni, Cr, Cu, Cd, Mn, Fe, and Hg in various organs of Wallago attu. Samples were collected from polluted parts of River Kabul and compared with control fish netted from Warsak Dam. The data indicated that the sequence of metal bioaccumulation in the skin, gills, and muscle was Zn > Cr > Pb > Cu > Ni > Fe > Mn > Hg > Cd, in the intestine was Zn > Pb > Cr > Cu > Ni > Fe > Mn > Hg > Cd, and in the liver was Zn > Pb > Cr > Cu > Ni > Fe > Mn > Hg > Cd. The overall metal burden in different tissues of W. attu was in the sequence of skin > gills > intestine > muscle > liver. The skin being the prime target organ showed that the route of metal uptake was the direct result of fish to metal exposure. The liver accumulated the least level of metals than other organs of the same fish.

Analysis of heavy metals in organisms based on an optimized quantitative LIBS

Organisms including human beings and animals suffer from the intrusion of chemical contaminants, e.g. heavy metals, from a variety of sources such as food, drinking water, breathing, and body contact. Heavy metals can impact strongly on proteins and various enzymes in organisms and deposit in biological tissues, causing chronic toxicosis. Quantitative analysis of the absorption of heavy metals in different organs and tissues of organisms is valuable in environmental biology. Laser-induced breakdown spectroscopy (LIBS) is a practical tool for quantitative analysis. Here, an optimized LIBS approach is proposed to analyze the contents of heavy metal elements in various tissues of a contaminated fish sample. In our experiments, some optimal experimental parameters of LIBS have been identified and an internal-standard-based quantitative analysis (ISQA) algorithm has also been adopted to determine the contents of some heavy metals. Experimental results show that heavy metals deposit selectively in certain fish tissues such as liver and gill rather than evenly distribute all over the fish body. The optimized quantitative LIBS approach can be promoted for the environmental biology.

Assessment of the quality of polluted areas based on the content of heavy metals in different organs of the grapevine (Vitis vinifera) cv Tamjanika.

In this study, the samples of the spatial soil and organs of the grapevine (Vitis vinifera) cultivar Tamjanika were collected from the selected zones near the Mining and Smelting Complex Bor (East Serbia). They were analyzed by ICP-OES to determine the content of Cu, Zn, Pb, As, Cd, and Ni with the aim of ascertaining if these data may help in the assessment and improvement of the quality of environment in polluted areas such as Bor and its surrounding area. The results obtained from the calculated biological and enrichment factors, as well as from the Pearson correlation study and hierarchical cluster analysis confirmed that very useful information is recorded in plant organs: root, stem, leaves, and fruit. Yet, when the atmospheric pollution is the sphere of interest, the most informative data are found in unwashed leaves. The results of this study indicated also that the investigated plant species has some highly effective strategies involved in tolerance to the stress induced by heavy metals, which makes it an excellent candidate for phytostabilization purposes. Planting of this grapevine cultivar can be recommended in all areas that are severely polluted with heavy metals.

English

Wild animals have provided complimentary protein for human populations across the world over the centuries. This study investigated on bioaccumulation of heavy metals in different organs of cane rat (Thryonomys swinderianus) and the health implications of its consumption. Four carcasses were collected from each of the four ecotomes (Mosinmi, Agbara, Omo forest reserve and Ibese) and concentrations of 7 heavy metals (Fe, Cu, Cd, Pb, Mn, Cr, Zn) were examined in four organs (skin, liver, lung and kidney) from each specimen used by Atomic Absorption Spectrophotometer. Analysis of variance revealed no significant difference (P>0.05) in the concentration of metals in the animal except Fe and Cu, while significant variation exists when specimens were compared across different ecotomes. Total mean concentrations were Fe (400.512±60.0107), Cu (8.569±1.0396), Cd (0.06±0.040), Pb (0.3156±0.1175), Mn (9.4200±1.0383), Cr (1.3013±0.2739) and Zn (72.771±10.5672). Average mean concentration for all the metals in the study area was found to be higher than the recommended level which suggests that consumption of animals from this ecotomes are hazardous to human health and no single organ is completely safe for human consumption. Key words: Bioaccumulation, Thryonomys swinderianus, cane rat, bush meat, heavy metals, wildlife consumption, animal toxicity.

Human health risks from metals and metalloid via consumption of food animals near gold mines in Tarkwa, Ghana: Estimation of the daily intakes and target hazard quotients (THQs)

Heavy metal and metalloid contamination in food resulting from mining is of major concern due to the potential risk involved. Food consumption is the most likely route of human exposure to metals. This study was therefore to assess metals in different organs and different animal species near gold mines used for human consumption (free-range chicken, goat and sheep) in Tarkwa, Ghana, and to estimate the daily intake and health risk. The concentrations of Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, and Pb were measured with an inductively coupled plasma-mass spectrometer and Hg analysis was done using the mercury analyzer. Principal component analysis of the results showed a clear separation between chicken, grouped on one side, and the ruminants clustered on another side in both offal and muscle. Interestingly, As, Cd, Hg, Mn and Pb made one cluster in the offal of chicken. Chicken muscle also showed similar distribution with As, Hg and Pb clustered together. The daily intake of metals (μg/kg body weight/day) were in the following ranges; As [0.002 (kidneys of goat and sheep)–0.19 (chicken gizzard)], Cd [0.003 (chicken muscle)–0.55 (chicken liver)], Hg [0.002 (goat muscle)–0.29 (chicken liver)], Pb [0.01 (muscles and kidneys of goat and sheep)–0.96 (chicken gizzard)] and Mn [0.13 (goat kidney)–8.92 (sheep liver)]. From the results, daily intakes of As, Cd, Hg, Pb and Mn in these food animals were low compared to the provisional tolerable daily intake guidelines. The THQs although less than one, indicated that contributions of chicken gizzard and liver to toxic metal exposure in adults and especially children could be significant.

Effect of Humic Acid and Bacterial Manure on Distribution of Heavy Metals in Different Organs of Maize

Effect of Humic Acid and Bacterial Manure on Distribution of Heavy Metals in Different Organs of Maize

Bioaccumulation of heavy metals in the Cyprinus carpio organs of the El Izdihar dam (Algeria)

The objective of this study is to determine the bioaccumulation of heavy metals in different organs of Cyprinus carpio of El Izdihar dam, Sidi Abdelli (Wilaya of Tlemcen) in northwestern Algeria. This latter is an important water resource for drinking water and irrigation in the region. The Zn, Pb, Fe, Ni, Cu, and Cd elements were analyzed using the Rayleigh WFX-130 atomic absorption spectrophotometer after wet digestion by Malayandi and Barette method. The results are given in mg/kg dry weight. One way ANOVA and principal component analysis (PAC) were used to compare the data among months (levels of 0.05). Mean concentrations were found to decrease in C. carpio samples in sequence in gills and gonads as Zn ##xgt; Fe ##xgt; Pb ##xgt; Ni ##xgt; Cu ##xgt; Cd, in fillets as Zn ##xgt; Pb ##xgt; Ni ##xgt; Fe ##xgt; Cu ##xgt; Cd. For Zn and Fe, bioaccumulation in the gills is higher than in the gonads and fillets. For Cu and Cd: fillets ##xgt; gills ##xgt; gonads, and for Pb and Ni: fillets ##xgt; gonads ##xgt; gills. In fish samples, the concentrations of zinc, lead, and cadmium exceed the FAO/WHO values. The results for all metals were statistically significant at p < 0.05 between different months and different organs.

Organ- and species-specific accumulation of metals in two land snail species (Gastropoda, Pulmonata)

In order to evaluate the usefulness of terrestrial gastropods as bioindicators there is a need for studies that simultaneously compare (1) concentrations of metals in reference and polluted plots, (2) species within the same polluted habitat, (3) metal accumulation patterns in different organs and (4) metal accumulation patterns in relation to soil physicochemical properties. This study aims to assess metal accumulation patterns in two land snail species. Instead of analyzing an organism as a whole, investigating the partitioning of metals in different organs can provide information on the actual toxicological relevant fractions. Therefore, concentrations of Ag, Cd, Cr, Cu, Ni and Zn were examined in five different organs of Cepaea nemoralis, as well as in the foot and the body of Succinea putris. Snails were sampled at four polluted dredged sediment disposal localities and three adjacent less polluted reference plots situated along waterways in Flanders, Belgium. Due to the small size and problematic dissection of S. putris only the concentrations in the foot of both species could be compared. For this reason only, C. nemoralis can be described as a better bioindicator species that allows a far more detailed analysis of organ metal accumulation. This study showed that organs other than the digestive gland may be involved in the immobilization and detoxification of metals. Furthermore, pH, soil fractionation (clay %, silt %, sand %) and organic matter, correlate with metal accumulation in organs. However, most often the soil metal concentrations did not correlate with the concentrations found in snail organs. Metal concentrations in organs of both species (1) differed among polluted plots but rarely between polluted and reference plots within a locality, (2) were organ-specific (digestive gland>foot>albumen gland=spermoviduct=ovotestis), (3) were species-specific and (4) depended on the metal type (high Cd and Cu concentrations were observed in the digestive gland and foot respectively). Our study emphasizes that background metal levels should be taken into account when using invertebrates as bioindicators of metal contamination and that bioindicators may show substantial differences in accumulation patterns even if they have a highly comparable ecology.

Bioaccumulation of Heavy Metals in Fish (Hydrocynus forskahlii, Hyperopisus bebe occidentalis and Clarias gariepinus) Organs in Downstream Ogun Coastal Water, Nigeria

In this study accumulation of some heavy metals Cadmium (Cd), Chromium (Cr), Cobalt (Co), Nickel (Ni) and Lead (Pb) in the operculum, gills, heart, kidney, muscle and vertebrae were determined in some fishes (Hydrocynus forskahlii, Hyperopisus bebe occidentalis and Clarias gariepinus) collected from fishermen around Ogun estuary. The accumulation of the metals in different organs showed significant differences (P H. bebe occidentalis > C. gariepinus and the pattern of distribution was Ni > Cr > Co > Cd > Pb for all the fish species. The levels of Ni and Cr in this study were higher than the maximum permissible limits (FAO, UNEP, FEPA and WHO) for human consumption and that of Cd, Pb and Co were still lower. Safe disposals of domestic sewage and industrial effluents as well as enforcement of laws enacted to protect our environment are therefore advocated.

Evaluation of Metal Concentrations in Red Tilapia (Oreochromis spp) from Three Sampling Sites in Jelebu, Malaysia Using Principal Component Analysis

Concentration of V, Mn, Fe, Co, Cu, Zn, As, Se, Cd, and Pb in muscle, liver, and gill tissues of red tilapia (Oreochromis spp) sampled from three different aquaculture sites which include earthen pond, ex-tin mining pool, and concrete tank in Jelebu, Negeri Sembilan, were determined using microwave-assisted digestion–inductively coupled plasma-mass spectrometry. Accumulation patterns relating organs and elements, as well as origins and elements, were evaluated using multivariate statistics. With the aid of principal component analysis, it is possible to visualize the distribution pattern of metals in different organs as well as clustering tendencies of tilapia samples according to the production sites. In general, levels of V, Co, Fe, Cu, Zn, Se, and Cd in liver were higher than those in muscles and gills, whereas Mn and Pb were higher in gills while As in muscles. Results from principal component analysis revealed that there are similar pattern of metal distribution among organs regardless of the production sites. It is also suggested that Cu, As, and Pb are the best describers in characterizing the studied organs, where liver tissues are associated with high Cu, gills with high Pb, and muscles with high As. On the other hand, V, Co, and Pb are observed to be key discriminants for sample origins.

Effects of rare earth elements on the distribution of mineral elements and heavy metals in horseradish

In order to investigate the effects of rare earth elements (REEs) on horseradish, the distribution of the mineral elements and heavy metals in different organs of horseradish have been studied by using inductively coupled plasma-atomic emission spectrometry (ICP-AES). Meanwhile, three variable major parameters, namely the concentration of REEs, the type of REEs, and the growth stage of plant were chosen. The results indicated that the test REEs, Ce(III) and Tb(III), could be accumulated in leaves, stems and roots of horseradish. In addition, we found that the content of mineral elements was increased in horseradish treated with 20 mg l −1 of Ce(III), but not those with the 20 mg l −1 of Tb(III). Moreover, the content of mineral elements in horseradish was decreased with the increasing concentration of REEs (100, 300 mg l −1). Furthermore, we found that there were the opposite effects on the content of the heavy metals in horseradish treated with REEs. Finally, we found that the effect of REEs on the accumulation of REEs, and the content of mineral elements or heavy metals of horseradish during vigorous growth stage, no matter positive or negative, was more obvious than that of the other growth stages. These results demonstrated that the distribution behaviors of mineral elements and heavy metals in horseradish can be affected by the type and concentration of REEs, and the growth period of plant.

Comparative study on the accumulation of heavy metals in different organs of tench (Tinca tinca L. 1758) and plerocercoids of its endoparasite Ligula intestinalis

Concentrations of heavy metals (Cu, Fe, Zn, Mn, Cr, Pb and Cd) in some organs of tench and tissues of its parasite found in Kovada Lake (Turkey) were analyzed using atomic absorption spectrophotometry and subsequently compared with the data from sediments and water. Only Cu, Fe, Zn and Mn were detected in water, sediment, plerocercoids of Ligula intestinalis and fish samples, while levels of Cr, Pb and Cd were below the detection limits. Four elements were found at higher concentrations in the plerocercoid than in different fish tissues (muscle, liver and gill), being 1.6-37.4 times higher than that measured in muscle, liver and gill. Significant positive (for Cu) and negative (for Fe, Zn and Mn) correlations were found between the quantity of heavy metals in water and tissues of L. intestinalis plerocercoids while there were significant positive (for Cu and Zn) and negative (for Fe and Mn) correlations between the quantity of heavy metals in bottom sediment and tissues of L. intestinalis plerocercoids. Cestodes were found suitable to reflect the amount heavy metals in sediments, providing more reliable information about the actual pollution of the reservoirs.

Contribution to the ecotoxicological study of cadmium, lead, copper and zinc in the mussel Mytilus edulis

The understanding of natural fluctuations of metal concentrations in mussels used as bio-indicators is indispensable for a good assessment of the disturbances due to pollution. We have studied thoroughly the factors which condition bioaccumulation by using controlled populations of mussels, Mytilus edulis L., sampled monthly over more than two years (March 1982–May 1984) in the Bay of Bourgneuf, France. Seasonal changes in metal levels have been recognized, maximum values being observed in winter and early spring and minimum in later spring and summer. Depending on metal and size group, the ratios between these maximum and minimum values varied between 1.56 and 3.43. The fluctuations in soft-tissue weight appear to be the main explanatory factor of seasonal variations in metal concentrations in mussels. Fluctuations in metal levels related to size of mussels were observed. Except for cadmium in mussels with a mean dry weight of soft tissues >0.2 g, a slight decrease in metal concentrations was observed for growing individuals: the regression coefficient b was -0.10, -0.11, -0.13 and -0.27 for Cd, Cu, Zn and Pb, respectively. The metals examined were rather uniformly distributed among the different groups of organs (visceral mass, gills and palps, “remainder”) and, consequently, several analyses of metals in different organs do not provide much more information than one analysis on the whole soft tissues. It is concluded that fluctuations related to size or season are reflected by only moderate differences in the maximum and minimum concentrations of metals in the mussels, but that they are nevertheless sufficient to conceal low chronic or short-term pollution, except at those sites where the “normal” environmental conditions are well-documented.