Metal Burden Research Articles

Quantification of 26 metals in human urine samples using ICP-MSMS in a random sample collective of an occupational and environmental health care center in Aachen, Germany

Despite several studies on metal exposure in the general population, the knowledge on the background burden of distinct metals is still sparse (e.g. Cu, In, Mn, Pb, Sn, Sr, Ta, Te). While up to date reference values exist for 16 distinct metals as Biological Reference Value (BAR) or the 95th percentile for Al, As, Ba, Be, Cd, Co, Cr, Hg, Li, Mo, Ni, Pt, Sb, Se, Tl and U respectively, the background burden of the general population for the remaining elements is unknown or yet no matter of scientific counselling. We established and validated an inductively coupled plasma triple quadrupole mass spectrometry (ICP-MSMS) human biomonitoring method (HBM), that enabled us to determine 26 metals in urine. Al, As, Ba, Be, Cd, Co, Cu, Ga, Gd, Hg, In, Li, Mo, Ni, Pb, Sb, Se, Sn, Sr, Ta, Te, Tl, V and Zn were analyzed. The method was applied to 88 urine samples collected in the ambulance of the Institute for Occupational, Social and Environmental Medicine (IASU) Aachen, Germany. Patients from two major metal processing companies (steel and copper) and a more heterogenous group of occupational exposed and non-exposed persons were defined and distinguished. HBM data from about 88, in general occupationally unexposed persons against certain metals served as a collective representing the general population in first approximation. For these the 95th percentiles are reported. Significant differences of urinary metal concentrations of the employees of the two metal processing companies compared to the third group were observed among others for Cu, Cr, Ni, Mn and are discussed, thus demonstrating the usefulness of the method for both environmental and occupational purposes.

Cd and Cr Metals Burden in Fruits and Vegetables of Southern European Countries: A Review

Cd and Cr Metals Burden in Fruits and Vegetables of Southern European Countries: A Review

Cd and Cr Metals Burden in Fruits and Vegetables of Southern European Countries: A Review

Cd and Cr Metals Burden in Fruits and Vegetables of Southern European Countries: A Review

Insights into the health status of the general population living near an electroplating industry zone: metal elevations and renal impairment.

A cross-sectional study was conducted in 2016 in Zhejiang Province, China, to evaluate the body burdens of metals and metalloids associated with renal dysfunction in populations living near electroplating industries. We recruited 236 subjects and performed physical examinations, determined the blood and urinary levels of arsenic (As), cadmium (Cd), chromium (Cr), manganese (Mn), nickel (Ni), lead (Pb), antimony (Sb), and selenium (Se) by an inductively coupled plasma mass spectrometer (ICP-MS), and measured three renal impairment biomarkers, namely nacetyl-β-D-glucosaminidase (NAG), retinol-binding protein (RBP), and β2-microglobulin (BMG). The proportion of abnormal nasal symptoms in the exposure group (10.1%) was much higher than in the control group (0; p < 0.05). The blood and urinary levels of As, Cd, and Se in the exposure group were significantly higher than those in the control group (p < 0.05). The blood levels of Mn and Pb, as well as the urinary levels of Cr and Ni, were significantly higher in the exposure group than in the control group (p < 0.05). The exposure group demonstrated higher levels of NAG, RBP, and BMG than the control group (0.51 vs. 0.14mg/g creatinine, 12.79 vs. 9.26IU/g creatinine, and 1.39 vs. 0.78mg/g creatinine, respectively; p < 0.05). Urinary BMG was positively correlated with urinary Cd levels (r = 0.223, p < 0.05), while urinary RBP was correlated with blood Cd levels (r = 0.151, p < 0.05) and urinary Cd, Cr, Ni, and Se levels (r = 0.220, 0.303, 0.162, and 0.306, respectively; p < 0.05). In conclusion, our study indicated that a population living in the vicinity of electroplating industries had high body burdens of certain metals and metalloids associated with non-negligible renal dysfunction.

Heavy metal contents in bovine tissues (kidney, liver and muscle) from Central Gondar Zone, Ethiopia

Environments prone to naturally non-biodegradable metal pollution have remained hot areas of research. Here, concentrations of Cu, Cr, Cd and Pb in 90 bovine tissue (kidney, liver and muscle) samples collected from Gondar City abattoir and Nara-Awudarda in Central Gondar Zone, Ethiopia, were investigated. The samples were dry-ashed at 550 °C for 3.45 h, mineralized with 2 mL conc. HNO3, and analysed using flame atomic absorption spectrometer. Pattern recognition methods, viz. principal component analysis (PCA) and partial least squares – discriminant analysis (PLS-DA), were employed to investigate heavy metal profiles in bovine tissues and classify them based on their geographic source. Results showed that the mean concentrations of the metals (mg/kg) extended in the range: 1.5–11.5 (Cu), 0.42–1.5 (Pb), BDL–1.05 (Cr) and BDL–0.41 (Cd). The concentrations of Cu and Cd, except Cd in muscle samples from Gondar City abattoir, were below the permissible limits while Pb and Cr, except for Pb in kidney and Cr in all samples at Gondar City abattoir, were above the permissible limits. Hence, it calls for enforceable food safety monitoring practices. PCA visualizes grouping of the studied bovine tissues based on the contents of analyzed metals where liver contained highest concentrations of the studied metals, except for Cd, which was highest in kidney. PLS-DA model clearly classified the tissue samples into two clusters depending on their geographic source. In general, the accumulation burden of heavy metals exhibited tissue-specific and sample source differences, probably due to varied organ functions and environmental conditions.

Investigation of the allergens in 2,316 children with allergic rhinitis from Guangdong, China.

Allergic rhinitis (AR) is one of the popular childhood diseases, bringing physical and metal burdens to the children and their families. The study was performed to detect common allergens eliciting AR in children, to investigate the prevalence of allergens in different age and gender cohorts, and to provide a reliable basis for clinical prevention and treatment of AR during childhood. We measured serum-specific IgE and performed inhalant and ingestion allergen examinations in 2,316 children with AR, in collaboration with BioSciTec GmbH. The prevalence of different allergens was determined according to gender, age, severity, and season. Among the 2,316 AR cases, the top five inhalant allergens were Dermatophagoides pteronyssinus (1,674 cases, 72.3%), Dermatophagoides farinae (1,520 cases, 65.6%), Blomia tropicalis (1,477 cases, 63.8%), Cockroach (602 cases, 26.0%), and Dog hair (602 cases, 26.0%). The top five ingestive allergens were Milk (1,111 cases, 48.0%), Egg white (543 cases, 23.4%), Shrimp/Crab (425 cases, 18.4%), Beef/Mutton (422 cases, 18.2%), and Egg yold (329 cases, 14.2%). AR severity analyses showed that 50.9% (1,180 cases) of D. pteronyssinus allergies were above level three, 47.9% (1,109 cases) of D. farinae allergies were above level three, only 23.3% (539 cases) of B.tropicalis allergies were level three, and B. tropicalis allergies were mainly of level 2. Other AR-inducing allergens mainly produced level one or two reactions. Regarding ingestion allergens, 7.9% (183 cases) of milk allergies and 4.7% (108 cases) of Shrimp/Crab allergies were above level three, and other allergens induced AR mainly of level one or two. The study investigated the major allergens eliciting AR in children from Guangdong, China, assessed the prevalence and severity among cohorts regarding age, gender, and season, and produced essential information on childhood AR, laying important references for AR prevention and treatment in the future.

Assessment of human exposure to food crops contaminated with lead and cadmium in Owerri, South-eastern Nigeria

BackgroundFood safety and security have remained an emerging global challenge amidst increasing human activities that potentially contaminate the food chain. With the rapid population growth, urbanisation and unrestrained emission of toxic substances, urban-dwelling Nigerians are particularly vulnerable to consuming contaminated food crops. Materials and methodThis study presents a framework for critical analysis of human exposure patterns to potentially contaminated food crops using the city of Owerri (Nigeria) as case study. It systematically assessed the metal burden of soil and staple food crops and the potential health risk associated with dietary exposure of humans to contaminated food crops. Samples of soil, cassava (Manihot esculenta) tubers and fluted pumpkin (Telfairia occidentalis) leaves were collected from household gardens and analysed for concentration of selected metals (Al, As, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Ni, Pb and Zn) using ICP-OES. A risk assessment of human exposure to Pb contamination using both the Target Hazard Quotient (THQ) and Hazard Index (HI) was estimated. ResultsThe majority of metals measured below the respective health-based maximum concentration limits of the Nigerian Department of Petroleum Resources target value, except for Pb in soil, which was above the limit of 85 µg g−1 in 0.9 % of sampled soils. However, Pb measured above the threshold of 0.3 µg g−1 stipulated by the FAO/WHO Codex Alimentarius in 46 % of sampled pumpkin leaves with no correlation established with soil Pb concentrations. This suggests possible Pb contamination via atmospheric deposition, and that human ingestion of pumpkin leaves presents the greatest health risks. ConclusionThe findings revealed that children and toddlers are more prone to Pb contamination via food crop ingestion than adults based on the THQ and HI evaluations; hence need for relevant policies to ensure food safety. This study provides background data for epidemiological investigation of relationships between contaminated food crop ingestion and blood Pb.

The effect of an organic food intervention treatment on biomarkers of exposure to lead and cadmium in primary school children of Cyprus: A cluster-randomized crossover trial

BackgroundFood contaminants, such as, pesticides and metals are ubiquitous in the food chain. Studies routinely report on the metals content of organic and conventional food crops. However, comparative human studies on the body burden of metals associated with organic food consumption are currently lacking. ObjectivesThe objectives were to i) determine the effectiveness of an organic food intervention in reducing the body burden of urinary concentration of lead (Pb) and cadmium (Cd) and ii) evaluate the association between metal exposures and biomarkers of oxidative damage in primary school children in Cyprus. MethodsThis study was part of the ORGANIKO cluster-randomized crossover trial, a 40-day organic food treatment to 149 healthy children (10–12 years) in Cyprus. Urinary biomarkers of Pb and Cd were determined using inductively coupled plasma mass spectrometry. Linear mixed-effect regression models were used to account for the effect and duration of the organic food treatment. Multiple comparisons were handled using Benjamini-Hochberg correction. ResultsA time-dependent reduction for creatinine-adjusted Pb during the intervention period was observed (β = −0.021; 95% CI: −0.034, −0.008; p-adjusted = 0.01). A similar trend was observed for creatinine-adjusted Cd, but it was not significant (β = −0.013; 95% CI: −0.026, 0.000; p-adjusted = 0.15). The creatinine-adjusted oxidative damage biomarkers were significantly associated with both metal biomarkers; Pb (8-OHdG: β = 0.388, 95% CI: 0.303, 0.472; p-adjusted<0.001; MDA: β = 0.187, 95% CI: 0.109, 0.265; p-adjusted<0.001; 8-iso-PGF2a: β = 0.320, 95% CI: 0.244, 0.397; p-adjusted<0.001), and Cd (8-OHdG: β = 0.148, 95% CI: 0.063, 0.233; p-adjusted = 0.003; MDA: β = 0.107, 95% CI: 0.030, 0.184; p-adjusted = 0.018; 8-iso-PGF2a: β = 0.263, 95% CI: 0.186, 0.339; p-adjusted<0.001). DiscussionA 40-day organic food treatment reduced primary school children's exposures to Pb over time. More human studies in settings with high food contaminant profiles across common crops are warranted.

Neuron Protection by EDTA May Explain the Successful Outcomes of Toxic Metal Chelation Therapy in Neurodegenerative Diseases.

Many mechanisms have been related to the etiopathogenesis of neurodegenerative diseases (NDs) such as multiple sclerosis, amyotrophic lateral sclerosis, Parkinson’s disease, and Alzheimer’s disease. In this context, the detrimental role of environmental agents has also been highlighted. Studies focused on the role of toxic metals in the pathogenesis of ND demonstrate the efficacy of treatment with the chelating agent calcium disodium ethylenediaminetetraacetic acid (EDTA) in eliminating toxic metal burden in all ND patients, improving their symptoms. Lead, cadmium, aluminum, nickel, and mercury were the most important toxic metals detected in these patients. Here, I provide an updated review on the damage to neurons promoted by toxic metals and on the impact of EDTA chelation therapy in ND patients, along with the clinical description of a representative case.

Occurrence and health implications of heavy metals in preschool children’s clothing manufactured in four Asian regions

Clothing may be a potential contributor to body metal burden in children. However, available information on heavy metals in children’s clothing is extremely limited and the associated health risks remain poorly understood. This study investigated the concentrations of Pb, Cd, Co, Zn, Cr, As, Cu and Ni in new preschool children’s clothing manufactured in four Asian regions. The children’s clothing had higher levels of Ni and Cr but lower levels of Pb and Cd in comparison to the concentrations reported in other textile products. The concentrations of Cd were higher in the black clothing than those in the white and color samples. The non-cotton samples contained higher Co concentrations. The Pb concentrations in the samples manufactured in China were significantly higher than those in the other three regions. We estimated the dermal exposure doses for these metals and calculated the associated risks. The results indicated that the health risks from exposure to these metals in the children’s clothing were acceptable. However, more research is required to investigate heavy metals and the associated risks in child clothing due to the increasing complexity of their materials and manufacturing processes.

Metallomics analysis for early assessment and individualized intervention of neurodevelopmental disorders

The children today are in the midst of the epidemic of neurodevelopmental disorders. In this metallomics study for the scalp hair samples of total 2550 children with autistic disorders (2108 males and 442 females aged 0-15 years), it was demonstrated that near one-half of the infantile individuals aged 0-3 years are suffering from zinc deficiency and toxic metal burdens. Zinc level correlated closely to the index of zinc/iron ratio more than zinc/copper ratio. Furthermore, there were significant relationships between zinc deficiency and toxic metal burdens such as lead and aluminum, which were inversely associated with not only zinc level but also zinc/iron ratio with higher regression coefficients of r = -0.486 and -0.551 (P<0.00001), respectively. High-significant inverse association was detected between zinc and molybdenum concentration (r = -0.509) and also between zinc/iron ratio and molybdenum (r = -0.548). These findings suggest that infantile zinc deficiency relates to the high burdens of not only toxic but also some essential metals such as molybdenum, iron, and manganese and that these various mineral imbalances play principal roles in the etiology of neurodevelopmental disorders. We expect that the early assessment and intervention of the mineral imbalances (or dis-homeostasis) in individual child open an avenue for evidence-based individualized treatment of neurodevelopmental disorders and also of the comorbid immune disorders, in near future.

Risk factor determination and qualitative risk assessment of Mucormycosis in Harbor Porpoise, an emergent fungal disease in Salish Sea marine mammals

Mucorales infections are increasing in frequency and are a One Health pathogen of concern. In humans and domestic animals, risk factors include being immunocompromised, elevated circulating serum iron, contaminated open wounds, or metabolic diseases such as ketoacidosis or uncontrolled diabetes. Mucormycosis was first identified in 2012 in Pacific Northwest marine mammals, predominantly in harbor porpoises. We performed an assessment to determine the overall qualitative risk, or risk score, of mucormycosis in harbor porpoises. Risk factors for this disease are unknown in aquatic mammals. In a separate risk factor analysis, potential risk factors such as pollutants, trace metals (e.g., iron), and co-infection with other pathogens (e.g., viruses and Brucella spp.) were examined in mucormycosis cases and noncases using a matched case-control study design, to determine the presence and strength of association of these factors with mucormycosis. Disease severity (gross and histopathology) and exposure scores were multiplied together to obtain the overall risk scores of 9 -16 which corresponded to moderate and severe, respectively. In the risk factor analysis, the factors most strongly associated with a mucormycosis case, relative to a control, were elevated liver iron, decreased blubber thickness, and the decreased ratio of the sum of PCB congeners/sum of PBDE congeners. The results of this study suggest that mucormycosis may pose an inordinately high risk to harbor porpoises (and potentially sympatric species in the Salish Sea such as southern resident killer whales) based on the detected prevalence and the severity of lesions observed at necropsy. However, the risk may be greater on an individual basis compared to the overall population, and is likely related to other factors such as increased POP and heavy metal burdens.

Ribosomal DNA copy number associated with blood metal levels in school-age children: A follow-up study on a municipal waste incinerator in Zhejiang, China

To evaluate the body burdens of heavy metals and explore the impact of environmental metal exposure on ribosomal DNA (rDNA) or mitochondrial DNA (mtDNA) copy number (CN) variation in school-age children living near a municipal waste incinerator (MWI), we conducted a follow-up study in 2019. A total of 146 sixth-grade children from a primary school located 1.2 km away from the MWI were recruited for our study. Metals, including vanadium (V), chromium (Cr), manganese (Mn), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), selenium (Se), cadmium (Cd), stannum (Sn), stibium (Sb), thallium (Tl), and lead (Pb), were determined by an inductively coupled plasma mass spectrometer method. Real-time qPCR was used to measure the rDNA and mtDNA CN. The blood metal levels followed this order: Zn > Cu > Se > Pb > Mn > Sb > As > Ni > Cd > Co > Cr > Sn > V > Tl. Blood Cr level was significantly correlated with 18 S, 2.5 S, and 45 S CN (β = −0.25, −0.22, −0.26, p < 0.05); Ni was correlated with 5 S (β = −0.36, p < 0.01); Cu was correlated with 28 S, 18 S, and 5.8 S (β = −0.24, −0.24, −0.23, p < 0.05); while Zn was correlated with 18 S, 5.8 S, and 45 S (β = −0.28, −0.32, −0.26, p < 0.05). In conclusion, school-age children living near the MWI had lower blood metal levels compared to children recruited in 2013, while rDNA CN loss was found to be correlated to several heavy metals in these children.

Heavy Metal Burden and Health Risk Assessment of Fresh, Frozen and Smoked Fish from a Local Market in Southwest Nigeria

Heavy metals load in fish environment and fish products had been an issue of public concern. The burden of some heavy metals in fresh tilapia (Oreochromis niloticus), frozen Herring (Clupea harengus), smoked herring, and associated risk to man was investigated. Atomic absorption spectrometry was used to determine metal concentrations. Standard risk measurement indices [daily metal intake (DMI), hazard quotient (HQ), and health risk index (HRI)] were used. Levels of Mn and Fe were significantly different (p&lt;0.05) among the fish species, while the burden of other metals was low to not detected in the samples. The level of Cu (0.11±0.04), Zn (2.51±0.82), and Fe (8.72±4.41) were recorded in the bones of smoked herrings, while the highest levels of Mg were recorded in fresh tilapia. Gills recorded significantly (p&lt;0.05) high levels of Mg (1.16±0.02), Fe (14.92±0.53), Cu (0.10±0.00), and Zn (1.35±0.04). The muscle of the frozen herring records the highest burden of these metals. Mn, Fe, Cu, and Cd revealed the highest HRI in all fish samples and age categories, especially for nine years old and below children. In conclusion, health fish indicators revealed that Mn, Fe, Cu, and Cd pose a risk to the populace and, with long time consumption, can do more damage to consumers, especially frozen herring.

Determination of toxic metal burden and related risk factors in pregnant women: a biological monitoring in Sabzevar, Iran.

Nowadays, the adverse effect of toxic metals on humans is well known, especially in the fetal period such as preventing cognitive development and congenital abnormalities of the central nervous system. Hence, this study aims to evaluate the toxic metal burden in mothers and newborns in Sabzevar. Obtained data can be useful for authorities in public health issues. To determine heavy metals in placental blood and umbilical cord blood, one hundred eighty blood samples were taken from ninety mothers referred to Shahidan Mobini Hospital for delivery. The amount of metals in samples was analyzed using inductively coupled plasma optical emission spectrometry (ICP OES). The results of this study revealed that 21.52%, 26.19%, and 60.71% of maternal blood samples (placental blood) and 16.47%, 56.47%, and 20% of umbilical cord blood samples were higher than the US center for disease control (CDC) recommended levels for Pb, Cd, and As respectively. According to the multiple linear regression analysis, the Pb (p = 0.054), As (p < 0.001), and Se (p < 0.001) levels had an association with the mother's living area. Also, there was a significant association between Se (0.021) and the age of the mother. However, the Se values in its optimum concentrations in the blood (60-140μg/L) can decrease the adverse effects of toxic metals, 72.5% of the pregnant women had Se values below the 60μg/L and only 6% of pregnant women had Se levels higher than 140μg/L. We concluded that the mothers inhabiting the rural areas need more Se sources in their diets.

Cohort Profile Update: the China Metal-Exposed workers Cohort Study (Jinchang Cohort).

The Jinchang Cohort was an ongoing 20-year ambispective cohort with unique metal exposures to an occupational population. From January 2014 to December 2019, the Jinchang Cohort has completed three phases of follow-up. The baseline cohort was completed from June 2011 to December 2013, and a total of 48 001 people were included. Three phases of follow-ups included 46 713, 41 888, and 40 530 participants, respectively. The death data were collected from 2001 to 2020. The epidemiological, physical examination, physiological, and biochemical data of the cohort were collected at baseline and during follow-up. Biological specimens were collected on the baseline to establish a biological specimen bank. The concentrations of metals in urine and serum were detected by inductively coupled plasma mass spectrometry (ICP-MS). The new areas of research aim to study the all-cases mortality, the burden of diseases, heavy metals and diseases, and the course of the chain from disease to high-risk outcomes using a combination of macro and micro means, which provided a scientific basis to explore the pathogenesis of multi-etiology and multi-disease and to evaluate the effects of the intervention measures in the population.

Impacts of trace metals on Roan Antelope, Hippotragus equines and its Endoparasite Strongyloides spp., sampled in the tropical rainforests of Odo Ona Kekere, Ibadan, Oyo State, Nigeria

BackgroundRoan antelopes, together with other mammals, serve as man's proteins. Parasitic infections affect their growth and development and therefore reduce their yield. Samples of hunted roan antelopes (Hippotragus equines) were collected from Oluwo bushmeat Market, Epe, and Odo Ona Kekere in Oluyole Local Government Area of Ibadan, Nigeria. The intestine and liver of the roan antelopes were assessed based on endoparasites, accumulated trace metals, lipid profile, antioxidant biomarkers, and histopathology. The study was conducted to determine the impacts of trace metals on the enteric parasite Strongyloides spp. as an early warning to metal toxicity impacts on the antelopes and the consumers. ResultsThe study showed that the enteric parasites of the Roan antelope accumulated barium at a higher level than the host. The parasites showed great potentials for storage of cadmium and nickel, with the second-highest bioaccumulation factors in the study (>2), after zinc with bioaccumulation factor > 3. Vanadium's significant bioaccumulation factors are recorded only in the liver and intestine of the roan antelopes. The negative impact of the multi-stress conditions was evident in this study. For example, the significantly highest concentrations of zinc and barium in the parasites than the intestines and liver of the roan antelopes may partly be implicated in the outstandingly higher cholesterol, and low-lipid lipoproteins indicate dyslipidemia, which results from cellular damage due to stress. In stress conditions, some physiological reactions occur, including changes in hormones and components in the blood. These events might lead to higher cholesterol levels which may result in dyslipidemia. As seen in this study, although the levels of MDA in the investigated tissues were reasonably fairly stable, the upregulated SOD in the investigated tissues of the parasite served as an early warning signal of stress in the roan antelopes. ConclusionThe study revealed that Strongloides spp might be a reliable bioindicator of the metal burden in the roan antelopes. The enteric parasite may also serve as a good biosequestration tool to alleviate the toxic load of cadmium and nickel from the roan antelope. The synergistic impacts of cadmium and nickel on the parasites might reduce the infection intensity in the host. This study has demonstrated an empirical early warning against the deleterious accumulation of vanadium, barium, and zinc, which might rise beyond acceptable levels in the future, thus providing prognostic data for proactive decisions by stakeholders to make pragmatic plans and policy towards a sustainable conservation of the roan antelopes.

Novel, parallel and differential synaptic architecture based on NAND flash memory for high-density and highly-reliable binary neural networks

A novel synaptic architecture based on a NAND flash memory structure is proposed as a high-density synapse capable of exclusive NOR (XNOR) operation for binary neural networks (BNNs). For the first time, a 4T2S-based synaptic architecture with a complementary input voltage that implements an equivalent bitwise XNOR operation is proposed. Two adjacent NAND flash strings connected with the word lines are used as one synaptic string with four input transistors connected to the bit-line. By changing the threshold voltage of the NAND flash cells and input voltages in a complementary fashion, the XNOR operation is successfully demonstrated. The large on/off current ratio (∼7 × 105) of the NAND flash cells and differential sensing scheme can implement high-density and highly reliable binary neural networks without error correcting codes (ECC), which can reduce the burden of the complementary metal–oxide–semiconductor (CMOS) overhead. Despite the string structure of NAND flash memory, the parallel read scheme significantly reduces the read-out latency when compared to the sequential read scheme. In addition, we show that with only 1 erase/program pulse, sufficiently low bit-error rate (7.6 × 10−9 %) is achieved without the conventional incremental step pulse programming (ISPP) scheme. Finally, the estimated synapse density of V-NAND flash memory with 128 stacks is ∼103 times that of the 2T2R synapse in resistive random access memory (RRAM).

Biochemical and reproductive biomarker analysis to study the consequences of heavy metal burden on health profile of male brick kiln workers

The present study aims to assess the effect of a heavy metal burden on general health, biochemical parameters, an antioxidant enzyme, and reproductive hormone parameters in adult male brick kiln workers from Pakistan. The study participants (n = 546) provided demographic data including general health as well as body mass index. Blood was collected to quantitatively assess hematological, biochemical, and reproductive hormone parameters as well as heavy metal concentrations using both atomic absorption spectroscopy (AAS) and particle-induced X-ray emission (PIXE). The data showed that 10% of the brick kiln workers were underweight and 10% obese (P = 0.059), with workers also reporting multiple health issues. Heavy metal concentrations utilizing AAS revealed significantly (p = 0.000) higher levels of cadmium, chromium, and nickel, while PIXE detected more than permissible levels of Si, P, S, Cl, K, Ca, Zn, Ti (p = 0.052), Mn (p = 0.017), Fe (p = 0.055), Co (p = 0.011), Ni (p = 0.045), and Cu (p = 0.003), in the blood of kiln workers. Moreover, a significant increase in platelet count (P = 0.010), a decrease in sodium dismutase levels (p = 0.006), a major increase in reactive oxygen species (p = 0.001), and a reduction in protein content (p = 0.013) were evident. A significant increase in cortisol levels (p = 0.000) among the workers group was also observed. The concentration of LH and FSH increased significantly (p = 0.000), while that of testosterone decreased (p = 0.000) in the worker group compared with controls. A significant inverse relationship was found between cortisol, LH (r = − 0.380), and FSH (r = − 0.946), while a positive correlation between cortisol and testosterone was also evident (r = 0.164). The study concludes that increased heavy metal burden in the blood of brick kiln workers exposes them to the development of general and reproductive health problems due to compromised antioxidant enzyme levels, increased oxidative stress conditions, and a disturbing reproductive axis.

A Cross‐Cutting Approach for Relating Anthropocene, Environmental Injustice and Sacrifice Zones

AbstractThe Anthropocene is an uneven phenomenon. Accelerated shifts in the functioning of the Earth System are mainly driven by the production and consumption of wealthy economies. Social, environmental and health costs of such industrialization, however, bear on low‐income communities inhabiting severely degraded territories by polluting activities (i.e., sacrifice zones). How global, national and local socio‐economic and governance processes have interacted in perpetuating socio‐environmental inequalities in these territories has been rarely explored. Here, we develop an historical quantitative approach integrating a novel chemostratigraphic record, data on policy making, and socio‐economic trends to evaluate the feedback relationship between environmental injustice and Anthropocene in sacrifice zones. We specifically outline a case study for the Puchuncaví valley ‐one of the most emblematic sacrifice zones from Chile‐. We verify an ever‐growing burden of heavy metals and metalloids over the past five decades paced by the staggering expansion of local industrial activities, which has ultimately been spurred by national and transnational market forces. Local poverty levels have declined concomitantly, but this path toward social equality is marginal as costs of pollution have grown through time. Indeed, national and international pollution control actions appear insufficient in mitigating the cumulative impact brought by highly toxic elements. Thus, our sub‐decadal reconstruction for pollution trends over the past 136 years from a sediment record, emerges as a science‐based tool for informing the discussion on Anthropocene governance. Furthermore, it helps to advance in the assessment of environmental inequality in societal models that prioritize economic growth to the detriment of socio‐environmental security.