1α,24(R)-Dihydroxyvitamin D3 [1α,24(R)(OH)2D3], a synthetic vitamin D3 analog, has been developed as a drug for topical use in the treatment of psoriasis. At present, the target tissue metabolism of 1α,24(R)(OH)2D3 is not understood completely. In our present study, we investigated the metabolism of 1α,24(R)(OH)2D3 in the isolated perfused rat kidney. The results indicated that 1α,24(R)(OH)2D3 is metabolized in rat kidney into several metabolites, of which 1α,24(R),25-trihydroxyvitamin D3, 1α,25-dihydroxy-24-oxovitamin D3, 1α,23(S),25-trihydroxy-24-oxovitamin D3, and 1α,23-dihydroxy-24,25,26,27-tetranorvitamin D3 are similar to the previously known metabolites of 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3]. In addition to these aforementioned metabolites, we also identified two new metabolites, namely 1α-hydroxy-24-oxovitamin D3 and 1α,23-dihydroxy-24-oxovitamin D3. The two new metabolites do not possess the C-25 hydroxyl group. Thus, the metabolism of 1α,24(R)(OH)2D3 into both 25-hydroxylated and non-25-hydroxylated metabolites suggests that 1α,24(R)(OH)2D3 is metabolized in the rat kidney through two pathways. The first pathway is initiated by C-25 hydroxylation and proceeds further via the C-24 oxidation pathway. The second pathway directly proceeds via the C-24 oxidation pathway without prior hydroxylation at the C-25 position. Furthermore, we demonstrated that rat kidney did not convert 1α-hydroxyvitamin D3 [1α(OH)D3] into 1α,25(OH)2D3. This finding indicates that the rat kidney does not possess the classical vitamin D3-25-hydroxylase (CYP27) activity. However, from our present study it is apparent that prior hydroxylation of 1α(OH)D3 at the C-24 position in the ‘R’ orientation allows 25-hydroxylation to occur. At present, the enzyme responsible for the C-25 hydroxylation of 1α,24(R)(OH)2D3 is unknown. Our observation that the side chain of 1α,24(R)(OH)2D3 underwent 24-ketonization and 23-hydroxylation even in the absence of the C-25 hydroxyl group suggests that 1α,25(OH)2D3-24-hydroxylase (CYP24) can perform some steps of the C-24 oxidation pathway without prior C-25 hydroxylation. Thus, we speculate that CYP24 may be playing a dual role in the metabolism of 1α,24(R)(OH)2D3.