Keratinocyte Metabolism Research Articles

In vitro and in vivo antiinflammatory activities of C10 substituted anthralin derivatives.

In model systems where anthralin and butanthrone have a high anti-proliferative activity, e.g., inhibition of keratinocyte metabolism in culture or inhibition of ornithine decarboxylase inductionin vivo, CD 003 is inactive. However, in the skin disease psoriasis there is both an inflammatory as well as a differentiation/proliferation component, and consequently we have evaluated anthralin and butanthrone for their potentialin vivo antiinflammatory activities. In addition, we have compared a new analogue, substituted at C10, with these two compound. Our results support the belief that modification of the anthralin structure can result in the formation of less toxic, less irritant and less staining analogues, which maintain or significantly improve upon the topical antiinflammatory properties of anthralin. The possibility that such analogues will be of use in the treatment of skin diseases with a major inflammatory component is at present being investigated.

Modulation of Phospholipid Metabolism in Murine Keratinocytes by Tumor Promoter, 12-O-Tetradecanoylphorbol-13-acetate

The possibility that phospholipid deacylation may be a critical event in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-associated effects on mouse skin prompted us to examine in vitro the effects of TPA on arachidonic acid metabolism in neonatal mouse keratinocytes. Three-day old neonatal keratinocytes were prelabeled with [14C]arachidonic acid ([14C]AA) and [14C] stearic acid ([14C]ST) and used to characterize the lipases that were activated when these cells were treated with TPA in culture. Data from these studies demonstrate that phosphatidylcholine (PC) and phosphatidylinositol (PI) are the major phospholipids that undergo early hydrolysis to release arachidonic acid when challenged by TPA. Of particular interest was the novel observation of the hydrolysis of 14C-labeled PI in these keratinocytes, the accumulation of [14C]1,2-diacylglyceride and the lack of the [14C]diacylglyceride phosphorylation to form [14C]phosphatidic acid. This lack of [14C] phosphatidic accumulation implied that although TPA enhanced the hydrolysis of [14C]PI resulting in increased [14C]diacylglyceride it did not enhance the resynthesis of the [14C]PI via the phosphorylation of the [14C]diacylglyceride. Therefore, TPA probably is not involved in the turnover of PI in these cells but is involved in the activation of PC hydrolyzing phospholipase A2 and PI hydrolyzing phospholipase C in these keratinocytes releasing arachidonic acid which then undergoes oxygenation reactions to provide biologically active eicosanoids.

Ultraviolet Radiation Induces Changes in Membrane Metabolism of Human Keratinocytes in Culture

Human keratinocytes in culture were prelabeled with [3H]arachidonic acid (AA) and then exposed to ultraviolet B radiation. Irradiated cells released labeled AA metabolites into media in a dose-dependent manner when compared to sham-irradiated cells. The response began immediately and continued for 24 h. Extracts from media were examined by high-performance liquid chromatography for identification of specific AA metabolites. Irradiated cells were stimulated to produce prostaglandin-like material (PGE2 and PGF2 alpha). These findings support the concept that the cell membrane of keratinocytes participates directly or indirectly in initiating the sunburn response. It is also felt that the metabolites formed following injury to the membrane are an integral component in the mediation of that response.

Melittin-stimulated arachidonic acid metabolism by cultured malignant human epidermal keratinocytes

Upon melittin stimulation, cultured SCC-13 keratinocytes release prostaglandins E 2, F 2α, 6-keto-F 1α, thromboxane B 2, leukotriene B 4, and 6-sulfido-peptide-containing leukotrienes (SRS) into serum free medium. Release of prostaglandins E 2, F 2α, and SRS, normalized to cell protein, is 3- to 10-fold higher from rapidly growing than confluent cultures. Cells growing with hydrocortisone in the medium produce approximately twice the level of the cyclooxygenase-mediated metabolites PGE 2 and PGF 2α as those without hydrocortisone, but similar levels of the lipoxygenase-mediated metabolite SRS. The results demonstrate the potential utility of squamous carcinoma lines for investigating biochemical pathways of arachidonic acid metabolism in keratinocytes.

NADH spectrofluorometry of rat skin.

ArticleNADH spectrofluorometry of rat skin.D J Pappajohn, R Penneys, and B ChanceD J Pappajohn, R Penneys, and B ChancePublished Online:01 Nov 1972https://doi.org/10.1152/jappl.1972.33.5.684MoreSectionsPDF (865 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByThe Effect of a 7-Week Training Period on Changes in Skin NADH Fluorescence in Highly Trained Athletes26 July 2020 | Applied Sciences, Vol. 10, No. 15The Effect of Exercise on the Skin Content of the Reduced Form of NAD and Its Response to Transient Ischemia and Reperfusion in Highly Trained Athletes15 May 2019 | Frontiers in Physiology, Vol. 10Responses of NADH to Physiological and Pathophysiological ConditionsMonitoring of Various Organs in Different Animal ModelsSpectroscopic Monitoring of NADH: Historical OverviewNoninvasive sorting of stem cell aggregates based on intrinsic markers17 January 2014 | Cytometry Part A, Vol. 85, No. 4In Vivo Multiphoton NADH Fluorescence Reveals Depth-Dependent Keratinocyte Metabolism in Human SkinBiophysical Journal, Vol. 104, No. 1Multiphoton Flow Cytometry to Assess Intrinsic and Extrinsic Fluorescence in Cellular Aggregates: Applications to Stem Cells5 August 2010 | Microscopy and Microanalysis, Vol. 17, No. 4Screening Approaches for Stem CellsLrrc10 is required for early heart development and function in zebrafishDevelopmental Biology, Vol. 308, No. 2Metabolic Mapping of MCF10A Human Breast Cells via Multiphoton Fluorescence Lifetime Imaging of the Coenzyme NADH3 October 2005 | Cancer Research, Vol. 65, No. 19Recovery of hemoglobin oxygen saturation and intrinsic fluorescence with a forward-adjoint model1 April 2005 | Applied Optics, Vol. 44, No. 10Tissue spectroscope: a novel in vivo approach to real time monitoring of tissue vitalityJournal of Biomedical Optics, Vol. 9, No. 5Real time micro-fiberoptic monitoring of endogenous fluorescence in the rat conceptus during hypoxiaTeratology, Vol. 48, No. 4In Vivo NADH FluorescenceLens redox fluorometry: Pyridine nucleotide fluorescence and analysis of diabetic lensExperimental Eye Research, Vol. 49, No. 3Two-dimensional analysis of the redox state of the rat cerebral cortex in vivo by NADH fluorescence photographyBrain Research, Vol. 119, No. 2 More from this issue > Volume 33Issue 5November 1972Pages 684-7 https://doi.org/10.1152/jappl.1972.33.5.684PubMed4344165History Published online 1 November 1972 Published in print 1 November 1972 Metrics