Metabolic Profile Parameters Research Articles

Ontogenetic features of hormonal status and metabolic profile of pigs in industrial technology

The hormonal and metabolic profile of conditionally healthy Yorkshire pigs of different age and sex groups was studied in an industrial pig farm. A statistically significant effect of age on the level of serum cortisol, triiodothyronine, and thyroxine in the studied pig groups was established. Increased cortisol levels compared to the literature data in almost all pig groups may indicate chronic stress, while increased secretion of thyroid hormones is an adaptive response associated with stress compensation. Biochemical parameters of blood serum in animals of different groups reflect age-related metabolic features. It was shown that the average parameter values for the groups as a whole are within the limits obtained by other authors. It was revealed that with age, there is a decrease in the content of glucose, triglycerides, and urea. At the same time, the level of total protein, albumins, and globulins in the blood serum of the studied pigs gradually increases with age. In some cases, reliable intersexual differences in the level of biochemical parameters were found, in particular, sows exceeded boars in cholesterol content, while the opposite pattern was noted for total protein. In general, the hormonal and metabolic profiles of the herd correspond to the “industrial” status of the complex. The ability to adapt, intensive protein, carbohydrate and fat metabolism are obviously fixed by targeted breed selection. The established reference values of the hormonal status and metabolic profile parameters can be used to characterize pigs of high-intensity meat breeds under industrial technology conditions.

Indicators of Glucose Metabolism in Children and Adolescents Characterized as Having "Metabolically Healthy" and "Metabolically Unhealthy" Obesity.

Some individuals with obesity may exhibit fewer metabolic disturbances and face a lower long-term risk of complications; however, the existence of this so-called "metabolically healthy obesity" (MHO) compared to "metabolically unhealthy obesity" (MUO) remains controversial. We hypothesized that children with MHO might have a more favorable profile than children with MUO. Markers of glucose metabolism and insulin sensitivity were compared between children and adolescents diagnosed with MHO and MUO. This study recruited prospectively 104 children and adolescents (aged 6-16 years, 47 boys) with obesity. All participants underwent an oral glucose tolerance test (OGTT), and a comparative analysis was performed on HOMA-IR, QUICKI, insulin sensitivity index (ISI), insulinogenic index (IGI), disposition index (DI), and oral disposition index (oDI). Glucose metabolism indices were compared in these subgroups according to pubertal status. Forty-seven children (45.2%) were diagnosed with MHO. The whole-body ISI differed significantly between the MHO and MUO groups (4.02 vs. 2.7, p < 0.01). The IGI was statistically lower in the MHO group compared to MUO (1.26 vs. 1.54, p < 0.01), while neither the DI nor the oDI differed significantly. A higher ISI (4.5 vs. 3.9, p < 0.01) was observed in prepubertal MHO individuals compared to MHO adolescents. Children classified as MHO according to the more recent criteria exhibit a more favorable metabolic profile than those with MUO. However, a completely healthy profile was not demonstrated in the MHO group, as many crucial metabolic profile parameters were comparable to those observed in the MUO group. The findings of this study indicate that all children with obesity, irrespective of whether they are categorized as having MUO or MHO, necessitate close monitoring.

Evaluation of pregnancy toxemia in goats: Metabolic profile, hormonal findings, and redox balance

Pregnancy toxemia entails the development of hyperketonemia due to the negative energy balance that occurs in the last trimester of pregnancy in dairy goats. There are a limited number of studies on new diagnostic biomarkers and the pathophysiology of pregnancy toxemia in goats. This study assessed the hormones spexin and irisin and redox biomarker and metabolic profile changes in goats with pregnancy toxemia. The study included 36 hair goats, with 12 in the subclinical pregnancy toxemia (SPT) group, 12 in the clinical pregnancy toxemia (CPT) group, and 12 in the control group (CG). Spexin, irisin, and insulin hormone concentrations were determined by ELISA, while metabolic profile parameters were evaluated with an automatic chemical analyzer and redox balance by spectroscopy. In the goats with pregnancy toxemia, serum levels of spexin, irisin, insulin, and glucose were significantly lower than those of CG goats. The SPT group had significantly higher ischemia-modified albumin concentrations than CG goats, while the CPT group had significantly lower native thiol concentrations compared to CG animals. The concentrations of beta-hydroxybutyric acid and non-esterified fatty acids in the groups with pregnancy toxemia were significantly higher than those of control animals. In the diagnosis of pregnancy toxemia, spexin had sensitivity of 90 %, specificity of 95 %, and an area under the curve value of 0.988, while irisin had sensitivity of 90 %, specificity of 91 %, and an AUC value of 0.979. In this study, both spexin and irisin hormones showed excellent performance in the diagnosis of pregnancy toxemia. In addition, pregnancy toxemia was found to cause increased oxidative stress and significant changes in metabolic and endocrine metabolism in goats. Measuring these biomarkers in both healthy dairy goats and those with pregnancy toxemia may contribute significantly to the management and diagnosis of herd health.

Effects of season on metabolic profile of Holstein Friesian cows in postpartum period

The aim of the present study was to determine the metabolic profile of Holstein-Friesian cows in the postpartum period, as well as the effect of season on metabolic profile. The postpartum period is essential in the reproductive life of high yielding dairy cows because of its impact on future gravidity. This study included 60 cows up to 15 days after parturition, aged 2-8 years (the largest number of cows was between 3 and 5 years old) with no apparent clinical problems. Cows were sampled in summer season (n=30) and winter season (n=30). Parameters of metabolic profile were determined as follows: glucose, albumin, total protein, cholesterol, bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), urea, calcium, phosphorus and magnesium. Statistical differences were considered significant at the p&lt;0.05. Present research showed that all investigated parameters were within a reference range for cattle. Impact of season sampling was determined for glucose, albumin, total protein, cholesterol and phosphorus, while bilirubin, calcium, magnesium, urea as well as activities of ALT, AST and LDH were unaffected by the season of sampling. In conclusion, metabolic status is affected by the season and examination during the postpartum period can provide valuable information of cows' health status, in order to diagnose and moreover prevent postpartum diseases.

Analytical and Clinical Interference of Sample Hemolysis in Evaluating Blood Biochemical and Endocrine Parameters in Cows.

Hemolysis is a common cause of errors in laboratory tests as it affects blood parameters and leads to a positive or negative bias. This study aims to examine the relationship between the level of hemolysis (expressed as cell-free hemoglobin concentration, g/L) and the variability of metabolic and endocrine parameters and to determine the threshold level of hemolysis that causes an analytically and clinically significant bias for the twenty most frequently examined blood parameters in cows. Paired blood samples of 10 mL each were obtained from 30 cows. One was subjected to mechanical trauma and plasma was extracted directly from the other. Hemolyzed and non-hemolyzed samples from the same animal were mixed to obtain final samples with cell-free hemoglobin concentrations of 0, 1, 2, 4, 6, 8, and 10 g/L. Metabolic and endocrine parameters were measured in the samples and their deviation and the linear equation between the level of hemolysis and the deviation were determined. The following threshold values of hemolysis were determined, which correspond to the acceptable analytical (lower value) and clinical (upper value) levels of parameter variability: BHB 0.96 and 4.81; NEFA 0.39 and 3.31; GLU 0.38 and 3.90; ALB 1.12 and 6.11; TPROT 1.40 and 6.80; UREA 6.62 and 20.1; TBIL 0.75 and 5.65; AST 0.11 and 2.18; GGT 1.71 and 8.90, LDH 0.01 and 0.11, ALP 0.97 and 2.95; TGC 1.56 and 15.5; CHOL 1.29 and 8.56; Ca 5.68 and 25.7; P 0.57 and 8.43; Mg 1.10 and 8.47; INS 1.15 and 3.89; T3 8.19 and 15.6; T4 8.97 and 18.5; and CORT 2.78 and 11.22 g/L cell-free hemoglobin. Three decision levels are available for each metabolic and endocrine parameter: if hemolysis is below the lower (analytical) threshold value, results can be reported without restriction; if hemolysis is between the lower and upper thresholds, the results can be issued with guidance in the form of corrective linear equations; and if hemolysis is above the upper (clinical) threshold, the results and sample must be discarded. This method contributes to an optimal approach to hemolysis interference with metabolic profile parameters in blood samples from cows.

Metabolic profile in first episode drug naïve patients with psychosis and its relation to cognitive functions and social cognition: a case control study

1st episode drug naïve patients with psychosis might be at higher risk for cardiometabolic disturbances which could affect the different cognitive, and executive functions and domains of social cognition. This study aimed to study the metabolic parameters in 1st episode drug naïve patients with psychosis, to evaluate the relation of these cardiometabolic domains to the cognitive, executive functions, and social cognition. Socio-demographic characteristics of 150 first episode drug naïve patients with psychosis and 120 matched healthy control groups were collected. The current study also assessed the cardiometabolic profile and cognitive functions in both groups. Social cognition was examined by Edinburgh Social Cognition Test. The study revealed a statistically significant difference in parameters of metabolic profile among the studied groups (p < 0.001*), the scores of cognitive and executive tests were statistically significantly different (p < 0.001*). In addition, the patient's group has lowered scores of domains of social cognition (p < 0.001*). Also, the mean affective theory of mind was negatively correlated with the conflict cost of the Flanker test (r = -.185* p value = .023). The total cholesterol level (r = − 0.241**, p value = .003) and level of triglycerides (r = − 0.241**, p value = 0.003) were negatively correlated with the interpersonal domain of social cognition, the total cholesterol level is positively correlated to the total score of social cognition (r = 0.202*, p value = 0.013). Patients with 1st episode drug naïve psychosis showed disturbed cardiometabolic parameters which have deleterious effects on cognitive functions and social cognition.

Effect of Helicobacter pylori eradication on metabolic profile: an international, multicenter, case-control study

As a gram-negative and microaerophilic bacterium, Helicobacter pylori (HP)is the main cause of chronic gastritis. Therefore, considering the high prevalence of HP infection worldwide, as well as the increasing prevalence of metabolic disorders, the present study aimed to investigate the relationship between HP infection eradication and metabolic profile. This prospective case-control study was performed on patients with HP infection whom referred to 7 medical centers in 3 countries (Iran, Egypt, and Vietnam) in 2020-2021. The metabolic profile of all of the participants evaluated before starting of treatment for HP eradication and 3 months after the treatment. Then changes of metabolic profile compared between those with successful HP eradication (group A) and subjects who failed to eradicate (group B). Overall, 199 patients, including 93 male (46.7%) with the mean age of 44.5 years (18-93 years) included. Based on response to treatment, the participants allocate into group A (those who respond to HP eradication): 164 cases (82.42%); or group B as those who failed to achieve eradication (35 cases, 17.58%). Racially 86.9% of participants were Caucasian and 89% diagnosed as non-ulcer dyspepsia (NUD). The most prevalent comorbidity include hypertension (11.5%) and hyperlipidemia (10%) which were more prevalent in group B (P = 0.002).Three months after therapy, average weight of participants among those who achieved eradication (group A) decreased from 73.1 to 71.4kg (P = 0.01), but in comparison with group B, was non-significant (P = 0.171). The BMI of patients before and after treatment did not show any significant differences. The biochemical parameters of patients before and after treatment were not significantly different regardless of treatment success (P > 0.05). The levels of total cholesterol and VLDL cholesterol after treatment were not significantly different from baseline values in two groups. HDL and LDL cholesterol levels before and after treatment in the resistant group were significantly higher than the responding group. Average serum TG level decreased significantly after treatment in the group A (P < 0.0001), in contrast to the resistant group (P = 0.356).The liver transaminases (AST and ALT) before and after treatment were not significantly different between the two groups (P > 0.05). The results of logistic regression showed that the eradication of infection has no significant affect any of the metabolic profile parameters. HP infection treatment in individuals without significant metabolic disorders does not affect metabolic parameters up to 3 months after eradication. HP eradication among subjects with several comorbidities mandates eradication protocol intensification to avoid treatment failure.

Metabolic profile in women differs between high versus low energy spenders during a low intensity exercise on a cycle-desk

Active-desks are emerging strategies aiming at reducing sedentary time while working. A large inter-individual variability in energy expenditure (EE) profile has been identified and has to be explored to better optimize and individualize those strategies. Thus the present study aimed at comparing the metabolic and physical profile of individuals characterized as high spenders (H-Spenders) versus low spenders (L-Spenders) based on EE during a cycle-desk low intensity exercise. 28 healthy women working in administrative positions were enrolled. Anthropometric, body composition and fasting metabolic profile parameters were assessed. EE was determined by indirect calorimetry, at rest and during a 30-min cycle-desk use. Participants were categorized as H-Spenders and L-Spenders using the median of the difference between EE at rest and during the 30-min exercise. H-Spenders had higher mean EE (p < 0.001) and carbohydrate oxidation (p = 0.009) during exercise. H-Spenders displayed higher values for fasting plasma insulin (p = 0.002) and HOMA-IR (p = 0.002) and lower values for HDL-cholesterol (p = 0.014) than L-Spenders. The percentage of body fat mass was significantly higher in H-Spenders (p = 0.034). Individuals expending more energy during a low intensity cycling exercise presented a less healthy metabolic profile compared with L-Spenders. Future studies will have to explore whether the chronic use of cycle-desks during work time can improve energy profile regarding metabolic parameters.

Effects of Supplementation with Rumen-Protected Choline and Methionine on Metabolic Profile and Some Reproductive Parameters in Dairy Cattle During Transition Period

This study conducted to determine effect of the rumen-protected choline and methionine supplementation to peripartum dairy cattle on metabolic profile, metabolic disorders and some reproductive parameters. For this, 3-6 year-old, multiparous, healthy and pregnant 32 high-yield Holstein dairy cows were divided into 4 different groups during trial as: a control (CON) group with no supplementation to the ration; a 75 gr/day rumen-protected choline chloride (CHOL) supplemented group, 42 gr/ day methionine (MET) supplemented group, 75 gr/ day choline chloride and 42 gr/ day methionine (MET + CHOL) supplemented group were during three weeks before and after parturation. Blood samples were taken at wk -3, calving, wk 3 and wk 10 and were analyzed. Health problems and reproductive data were recorded during treatment. Statistically significant differences were detected in non-esterified fatty acids (NEFA), insulin like growth factor-1 (IGF-1), glucose, total protein, albumin, direct bilirubin, total cholesterol and very low density lipoproteins (VLDL). Results of this study suggested that rumen-protected choline and methionine supplementation to the rations of dairy cattle affected some metabolic profile parameters. The statistical differences in metabolic profile tests did not affect metabolic diseases and reproductive parameters.

Animal Biomonitoring for the Surveillance of Environment Affected by the Presence of Slight Contamination by β-HCH.

The aim of this study was to evaluate the influence of hidden environmental pollution on some blood parameters of sheep to detect susceptible biomarkers able to reveal slight contamination. Four dairy sheep farms, two with semi-extensive and two with intensive type systems were involved in this study. Two farms in different systems were chosen as properly located in a southern area of Latium (Italy), close to the Sacco River, in which contamination with β-hexachlorocyclohexane (β-HCH) occurred in the past due to industrial waste. A recent study established the presence of low but detectable residual contamination in these areas. The other two farms were outside the contaminated area. Erythrocyte glutathione transferase (e-GST) and oxidative stress parameters were monitored as well as some immune response and metabolic profile parameters throughout the investigated period of four months. The present study showed a relevant and significant increase in e-GST (+63%) in the extensive farming system of the contaminated area, whereas some immune response biomarkers, i.e., white blood cells, neutrophils, lymphocytes, and lysozyme resulted within the physiological range. In all farms, oxidative stress and acute phase response parameters were also within the physiological range. Our results suggest that e-GST is a very effective alarm signal to reveal “hidden” persistent contamination by β-HCH, and reasonably, by many other different dangerous pollutants.

Oxidative status in dairy goats: periparturient variation and changes in subclinical hyperketonemia and hypocalcemia

BackgroundA better comprehension of the redox status during the periparturient period may facilitate the development of management and nutritional solutions to prevent subclinical hyperketonemia (SCHK) and subclinical hypocalcemia (SCHC) in dairy goats. We aimed to evaluate the variation in the redox status of dairy goats with SCHK and SCHC during their periparturient periods. Guanzhong dairy goats (n = 30) were assigned to SCHK (n = 10), SCHC (n = 10), and healthy (HEAL, n = 10) groups based on their blood β-hydroxybutyrate (BHBA) and calcium (Ca) concentrations. Blood were withdrawn from goats every week from 3 weeks before the expected parturition date to 3 weeks post-kidding. On the same day, the body condition scores (BCS) were evaluated, and the milk yield was recorded for each goat. The metabolic profile parameters and the indicators of oxidative status were determined by using the standard biochemical techniques.ResultsIn comparison with the HEAL goats, SCHK and SCHC goats presented with a more dramatic decline of BCS post-kidding and a significant decrease in the milk yield at 2- and 3-weeks postpartum, ignoring the obvious increase at 1-week postpartum. The levels of non-esterified fatty acids (NEFA) peaked at parturition, exhibiting significantly higher levels from 1-week prepartum to the parturition day in the SCHK and SCHC groups. The malondialdehyde (MDA) concentration was increased in the SCHK goats from 1-week antepartum until 3-weeks postpartum, with its concentration being significantly higher in the SCHC goats at parturition. The hydrogen peroxide (H2O2) concentration was significantly lower in the SCHK and SCHC goats from 2-weeks antepartum to 1-week post-kidding. The total antioxidant capacity (T-AOC) and the superoxide dismutase (SOD) level were decreased at 1-week antepartum in the SCHK and SCHC goats, respectively. The glutathione peroxidase (GSH-Px) level was increased in the SCHK and SCHC goats during the early lactation period.ConclusionsThe SCHK and SCHC goats exerted more efforts to maintain their redox homeostasis and to ensure the production performance than the HEAL goats during their periparturient period, probably owing to more intense fat mobilization and lipid peroxidation in the former.

Influence of Season and Lactation Stage on Oxidative, Haematological, and Metabolic Profile Parameters in Holstein Dairy Cows

Influence of Season and Lactation Stage on Oxidative, Haematological, and Metabolic Profile Parameters in Holstein Dairy Cows

Evaluation of some metabolic profile parameters in transition cows: thresholds for estimating postpartum diseases in Aydin province

This study aimed to evaluate non-esterified fatty acids (NEFA), beta hydroxybutyric acid (BHBA), magnesium (Mg2+), calcium (Ca2+) and lactate as biological tests for the detection of some metabolic diseases during transition period in selected dairy farms located in Aydın province. Cow-side analysis with body condition score evalutation were performed weekly in at least 12 dairy cattle from each farm from prepartum week 2 to postpartum week 2. According to the prepartum NEFA ≥0.4 mmol/L, postpartum BHBA ≥1.4 mmol/L and Ca2+ ≤ 2.0 mmol/L at parturition (day 0) in herd-based evaluation, it was observed that all farms were positively evaluated for negative energy balance and also at risk for subclinical hypocalcaemia. Regarding the threshold value of Mg2+ &lt;0.61 mmol/L, subclinical hypomagnesaemia was observed at the Ist and IInd farms. It was concluded that prepartum NEFA threshold (0.68 mmol/L), obtained from the receiver operating characteristics statistical analysis could be used for observing some postpartum clinical diseases. It was suggested that it allowed planning and strategic interventions to prevent herd-based diseases in Turkey and will be a precedent for future studies and herd management.

Changes in antioxidant enzyme activities and metabolic parameters in dairy cows during different reproductive periods

Our study aimed to monitor the alterations in antioxidant enzyme activities and the metabolic profile parameters and their relationships in dairy cows during different reproductive stages (gestational-, dry-, pre- and post-partum). The collection of blood samples from thirty healthy pregnant Holstein cows took place at a commercial dairy farm, between September 2015 and June 2016. The cows covered eight different reproductive stages (4–6 weeks before the dry period (D4-6w)), at the beginning of the dry period (D0; on day 210 of gestation), the first month of the dry period (D1m), antepartum day 8 (APd8), postpartum (PP) day 3 (PPd3), PP day 8 (PPd8), PP between 3rd-4th weeks (PP3-4w) and PP between 80 and 90 days (PP80-90d). The activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were analysed to monitor and evaluate the antioxidant system. Concentrations of beta-hydroxybutyrate (BHBA), non-esterified fatty acids (NEFA), albumin, glucose, total bilirubin, cholesterol, calcium (Ca) and the enzyme activities of aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), gamma-glutamyl transferase (GGT) were also determined as metabolic profile parameters. GPx activities increased in D1m (P < 0.05) and peaked during the PP3d and PP8d periods (P < 0.0001), with values decreasing again at the PP3-4w period. The most significant change in SOD activities was detected in the PP3-4w with a significant decrease (P < 0.05). BHBA and NEFA concentrations increased significantly during the PPd3 and PPd8 periods (P < 0.05), with BHBA decreased to low values in the PP3-4w (P < 0.05). Significant positive correlations were found between BHBA, NEFA, total bilirubin and GPx (0.784, 0.874 and 0.871; P < 0.05, P < 0.01 and P < 0.01 respectively). A significant negative correlation between calcium and cholesterol and GPx was found (−0.857, P < 0.01; and −0.681, P < 0.05, respectively). As a result, a relationship was determined between blood antioxidant enzymes and metabolic parameters at different periods. High GPx and SOD activities, especially in the early period before and after parturition, is thought to be related to the adaptation of the animals to this process. The lack of adaptation abilities of animals during these periods causes many problems seen during early postpartum. Therefore, considering GPx and SOD activity measurements in pre- and postpartum periods contributes to better management of these periods and the prevention of complications.

Prothrombotic fibrin network characteristics in patients with acromegaly: a novel mechanism for vascular complications.

There remains increased cardiovascular mortality in patients with acromegaly. This study aims to evaluate whether GH/IGF-1 excess increases vascular disease by adversely affecting fibrin network characteristics. Cross-sectional study in 40 patients with acromegaly (21 males, age 53 ± 13 years) and 40 age/gender-matched controls. Clot structure was analysed using a validated turbidimetric assay and fibrin networks were visualised by laser scanning confocal microscopy (LSCM). Metabolic profile parameters, body composition, plasma fibrinogen and PAI-1 were also assessed. Twenty-two patients had active acromegaly and 18 were in remission. There was no difference in qualitative patient characteristics between the two groups. Both groups had less favourable body composition and cardiovascular risk profile compared with controls. Despite no difference in clot formation and lysis parameters between the two patient groups, active disease patients had higher fibrinogen and clot maximum absorbance compared with controls, after adjusting for BMI (3.8 ± 0.2 vs 2.6 ± 0.2 mg/mL, P < 0.001; and 0.39 ± 0.02 vs 0.33 ± 0.01 arbitrary units, P = 0.03, respectively). Patients in remission had higher fibrinogen compared with controls following adjustment for BMI (3.3 ± 0.2 vs 2.6 ± 0.2 mg/mL, P = 0.02) but not clot maximum absorbance (0.35 ± 0.03 vs 0.33 ± 0.02 arbitrary units, P = 0.6). LSCM showed increased fibrin network density only in active disease patients, consistent with turbidimetric analysis. In addition to active disease, BMI, fat mass and skinfold thickness were associated with higher clot density and longer lysis time. Patients with active acromegaly have more compact clots, thus conferring increased thrombosis risk. Prothrombotic fibrin networks may represent one mechanism for enhanced vascular risk in active acromegaly.

Blood metabolic profile tests at dairy cattle farms as useful tools for animal health management

Blood metabolic profile tests are simple cost-effective biochemical tests which are mostly used to identi-fy nutritional and/or management challenges in dairy cattle herds, but they also can be simply used to find animals which are clinically healthy, but really have some hidden problems like low production performance, reproductive diseases and/or long calving intervals and other sub-clinical diseases. Rou-tine metabolic profile tests commonly consist of blood glucose, urea, albumin, cholesterol, beta-hydroxybutyric acid and non-esterified fatty acids values, as well as sodium, potassium, chloride, calci-um, magnesium and inorganic phosphate levels. Briefly, the current review discusses blood metabolic profile tests, their importance, introduces an easy method for it, emphasises on the relation between blood metabolic profile parameters and many important sub-clinical diseases including ketosis, milk fever, mastitis, cystic ovaries, displaced abomasum and etc., and indicates that because of its simplicity, low cost and easy for analysis results, it can be considered as a good method for diagnosis of important diseases at dairy cattle farms.

Impact of vitamin D3 status on major clinical and laboratory parameters of patients with non-alcoholic fatty liver disease

Non-alcoholic fatty liver disease (NAFLD) is a non-specific, integral and multifactorial organ damage in type 2 diabetes mellitus (T2DM), obesity, insulin resistance (IR), metabolic syndrome. Recently, vitamin D3 deficiency is considered one of the factors associa­ted with the development of NAFLD. Objective — to evaluate the functional state of the liver, steatosis indices, and metabolic profile parameters in patients with NAFLD depending on the availability of vitamin D3 to the patient’s body. Materials and methods. Аccording to the recom­men­dations of the European Association of Endocri­nologists, all patients were divided into 3 groups: group 1 — with an optimal level of vitamin D3 (30 ng/ml) group 2 — D3 insufficiency (21—29 ng/ml) and group 3 — D3 deficiency (< 20 ng/ml). The diagnosis of NAFLD was made on the basis of clinical analysis, transaminase activity and ultrasound examination. Results and discussion. The study included 126 patients with T2DM and concomitant NAFLD. Over 90 % of all examined showed overweight or obesity of varying degrees, and BMI and WC increased in parallel with a decrease in the concentration of vitamin D3. There was also a tendency to an increase in transaminase activity in parallel with a decrease in vitamin D3 levels, however, no significant difference between the groups was revealed. The maximum value of fatty hepatosis indices was diagnosed in patients with D3 deficiency, which was statistically significantly different from patients from the optimal support group (HSI — 43.34 ± 6.59 versus 39.67 ± 4.37; p = 0.032 and, respectively, for FLI — 79.21 ± 19,61 versus 64.96 ± 17.72; p = 0.007). For the TyG index, a tendency to its increase was demonstrated at the same time as the D3 status worsened (p = 0.175). The subanalysis found that the pronounced D3 deficiency (< 10 ng/ml) is characterized by statistically significantly higher indicators of fatty hepatosis indices, anthropometric parameters and transaminase activity compared with patients with a D3 level in the range of 10—20 ng/ml. Conclusions. Vitamin D3 deficiency in patients with NAFLD is characterized by statistically significantly higher indices of fatty hepatosis indices, anthropomet­ric parameters and transaminase activity compared to the optimal level or its insufficiency.

Gene expression pattern of acetyl-coA carboxylase alpha, fatty acid synthase, and stearoyl-CoA desaturase in pregnant Barki sheep under complete feed deprivation

Objective: To assess the mRNA level of acetyl CoA carboxylase alpha (ACACA), fatty acid synthase (FASN), and stearoyl-CoA desaturase (SCD) by means of real-time PCR in Barki sheep subjected to complete feed deprivation. Design: Controlled study. Animals: Seven healthy pregnant ewes. Procedures: Ewes were subjected to complete feed deprivation with ad libitum water for five consecutive days. Venous blood samples were collected from each ewe before (zero time) and on the fifth day post-deprivation of feed for measurement of the mRNA level of ACACA, FASN, and SCD and assessment of serum metabolic profile parameters. Results: On the fifth day post-fasting, the gene expression pattern of ACACA, FASN, SCD genes showed a significant (p &lt; 0.05) down regulation in comparison with pre-deprivation of feed. There was a significant (p &lt; 0.05) increase in the serum level of non-esterified fatty acids (NEFA), beta-hydroxyl buteric acid (BHBA), and triglycerides in pregnant ewes in the fifth day post-fasting in comparison with pre-deprivation of feed. On the other hand, there was a significant (p &lt; 0.05) decrease in the level of glucose, cholesterol, and insulin in pregnant ewes in the fifth day post-fasting compared with pre-deprivation of feed. On histopathology, liver showed marked heptic steatosis in midzonal and periportal area, with formation of small fatty cysts in liver lobule. There was a positive correlation between leptin and insulin (r = 0.996; p &lt; 0.01), BHB and leptin (r = 0.951; p &lt; 0.05) and glucose and SCD (r = 1.0, p &lt; 0.01). However, there was a negative correlation between FASN and NEFA (r = - 0.991; p &lt; 0.05), FASN and leptin (r = -0.683; p &lt; 0.05) and FASN and cholesterol (r = - 0.82; p &lt; 0.05). Conclusion and clinical relevance: Pregnant Barki ewes can clinically tolerate complete feed deprivation for five days, with down regulation of ACACA, FASN, SCD genes and presence of marked metabolic changes. Therefore, metabolic monitoring is warranted to predict the early changes associated with feed deprivation under different stressful conditions.

SUN-075 Higher Fibrinogen and Clot Density in Patients with Acromegaly: The Role of Adverse Body Composition to the Increased Thrombotic Potential

Introduction: Patients with acromegaly have increased mortality rates compared with the general population, particularly due to cardiovascular and cerebrovascular disease. We hypothesized that one mechanism through which GH/IGF-1 excess increases vascular disease is via effects on hemostasis/fibrinolysis and subsequent clot formation. Patients and Methods: We conducted a case-control study in 40 patients with acromegaly and 40 age and sex-matched healthy controls. We measured parameters of metabolic profile, fibrinogen using the Clauss method and performed clot structure analysis using a validated turbidimetric assay. Results: Forty patients with acromegaly (21 males, mean age 53±13 years) were recruited. 30% had active disease (GH>1mcg/L and IGF-1 above the reference range), 27.5% had dichotomous GH/IGF-1 results and 42.5% were in remission. Compared with controls, patients had a less favorable metabolic profile with higher BMI (30±5.5 vs. 26.7±4.1 kg/m2, p=0.003), waist/hip ratio (0.91±0.08 vs. 0.87±0.08, p=0.045), total fat mass (29.8±10 vs. 23.4±10 kg, p=0.003) and triglycerides (1.5±0.9 vs. 1.1±0.5 mmol/L, p=0.02). Significantly higher fibrinogen levels were found in the patient group (3.59±1.56 vs. 2.58±0.37 mg/ml, p<0.001). Patients had raised clot maximum absorbance (a measure of clot density), compared with controls (0.38±0.13 vs. 0.32±0.08 arbitrary units, p=0.02). Regarding fibrinolysis, no statistical difference in clot lysis times was found between the two groups, however patients with acromegaly had numerically more prolonged lysis times compared with controls at all lysis stages (times from maximum clot optic density to 25%, 50% and 75% lysis were respectively: 15.5±16.6 vs. 10.4±5.4 min, p=0.067; 29±22.2 vs. 23.2±11.8 min, p=0.15; and 40.8±22.5 vs. 35.3±12.5 min, p=0.18). In the patient group smoking was associated with higher fibrinogen levels (coefficient 0.8, p=0.03), higher clot density (coefficient 0.07, p=0.035) and reduced lag time for clot formation (coefficient -39.3, p=0.02). Diagnosis of diabetes, higher BMI and total fat mass were associated with more prolonged lysis time (coefficients 13, p=0.04; 1.7, p=0.007; and 0.9, p=0.009 respectively). Total fat mass was also positively associated with higher clot density (coefficient 0.005, p=0.01). Additionally, a positive correlation was found between fibrinogen and clot density (coefficient 0.05, p<0.001). Conclusions: Patients with acromegaly demonstrate abnormalities in clot structure properties, particularly in clot formation, with higher fibrinogen and clot density, suggesting increased thrombotic potential with more compact clots. The adverse metabolic profile of these patients is a contributing factor to these clotting abnormalities. Prospective studies are required to assess whether hemostatic abnormalities in acromegaly are reversible after successful treatment.

Insulinska rezistencija kod krava u peripartalnom periodu (pregled)

Insulin resistance is a state in which the biological effect of insulin is reduced when its concentration decreases or when a compensatory mechanism increases its concentration. Insulin resistance is characterized by reduced insulin response to glucose, i.e. decreased pancreatic beta cell function (insulin hyporesponsiveness) and/or reduced sensitivity of glucose to insulin (reduced intake of glucose by peripheral tissues under the influence of insulin - eng. insulin sensitivity). Methods for estimating insulin resistance include direct methods (hyperinsulinaemic-euglycaemic clamp, golden standard), indirect methods (intravenous glucose tolerance test) and surrogate methods (indices calculated from basal concentrations of insulin, glucose, NEFA and BHB-HOMA, QUICKI, RQUICKI and RQUICKI- BHB). Surrogate indices show correlations with direct and indirect test results but they are inconsistent. Inconsistency occurs because the dependence of glucose concentrations on the degree of hepatic gluconeogenesis should be kept in mind when evaluating insulin resistance in ruminants. Therefore, the hyperinsulinaemic-euglicaemic clamp method is particularly suitable as it excludes gluconeogenesis in hepatocytes from analysis. Our results have shown a correlation between HOMA, QUICKI and RQUICKI indices and metabolic profile parameters. The correlation between dynamic and basal responses of NEFA, BHB, insulin, glucose and inorganic phosphorus is significantly dependent on RQUICKI-BHB index values in ketotic cows. RQUICKI index of insulin resistance is substantially dependent on NEFA values in early lactation as well as on insulin and glucose values in the dry period. Further investigations should focus on the practical evaluation of the use of insulin resistance indices.