Mesoporous SiO2 Research Articles

Ultra‐Stable and Bright Pure‐Red Perovskite Nanocrystals for Backlit Displays

AbstractMetal halide perovskite nanocrystals (NCs) have emerged as an alternative to conventional phosphors for wide color gamut displays. However, the issues of poor stability and low emission efficiency of pure‐red CsPbBrxI3‐x NCs set an obstacle to their practical application. Herein, the synergistic effect of Mn doping and mesoporous SiO2 sealing is reported through in situ formation of Mn‐doped NCs into mesoporous SiO2 nanospheres (MnNCs@SiO2) to structurally and spatially stabilize the perovskite NCs for bright and stable pure‐red emitter. The large bonding energy of Mn‐halogen effectively suppresses the halide vacancy defects, prompting the highest photoluminescence quantum yield (PLQY) up to 84% (634 nm) among the pure‐red composite analogs. More importantly, the nanocrystal structure stability is fundamentally improved by the enhanced formation energy with Mn doping, together with the spatial isolation by SiO2 nanospheres. The MnNCs@SiO2 films exhibit impressive stabilities against high‐temperature heating, thermal cycle test, UV irradiation, water, and acid/alkali erosion, representing one of the most stable pure‐red perovskite emitters. By integrating fabricated all‐perovskite CsPbX3 single color conversion film into the liquid crystal display (LCD) modules, a wide color gamut of 128% of NTSC is achieved, enabling an excellent color rendition for high‐saturation object colors toward practical applications.

Enhanced methanol formation in CO2 hydrogenation through synergistic copper and gallium interaction

Methanol production from CO2 hydrogenation has attracted great attention, due to the demand for carbon neutralization. Ga-promoted Cu catalysts show promising properties for methanol formation in CO2 hydrogenation, urging a fundamental investigation of the active state. In this study, we synthesized CuGa species on mesoporous SiO2, varying the amount of Ga. The structural characterizations revealed that Ga existed mostly in highly dispersed GaOx species with Cu in the form of metallic Cu clusters. The addition of Ga significantly increased the methanol formation with an optimum Ga value compared to Cu and Ga on SiO2. The surface-sensitive measurements showed the mixed form of Cu+ and Cu0 on the Cu surface exhibited, where the Cu+ state was proportional to the methanol formation rate. Therefore, we conclude that the Cu+-GaOx interface constitutes an active site for methanol production. Furthermore, we found that the Ga addition influences the oxidation state and dispersion of Cu.

Extraction and green template-free synthesis of high-value mesoporous γ-Al2O3 and SiO2 powders from coal gangue

The study successfully demonstrated an eco-friendly approach for synthesizing mesoporous alumina and mesoporous silica from coal gangue, in line with green chemistry rules. By employing natural breakdown and increased dissolution of coal gangue through specific chemical treatments, along with optimal leaching conditions, this study achieved a dissolution efficiency of over 98% for aluminum. This process, which includes leaching, separation, simultaneous precipitation, selective dissolution, gel formation, and calcination, produced mesoporous alumina and mesoporous silica without the need for templates. The results presented that the synthesized mesoporous alumina and silica had purities of 98.81% and 99.08%, respectively. Additionally, the synthesized mesoporous alumina and silica had specific surface areas of 340.15 and 392.85 m2/g, and average pore sizes of 7.27 and 3.25 nm, confirming the desirable physical properties of the synthesized γ-Al2O3 and SiO2. These products offer a sustainable solution to environmental issues caused by coal accumulation, with promising applications across various industries.

Aptamer molecular gate functionalized mesoporous SiO2@MB controlled-release system for pollutant detection using Ti(Ⅲ) self-doped TiO2 NTs as active photoanode coupled with electrostatic modulation

Atrazine (ATZ) is a widely used herbicide that can cause serious harm to organisms and ecosystems. An immobilization-free photoelectrochemical (PEC) aptasensor has been herein developed for ATZ based on aptamer molecular gate functionalized mesoporous SiO2@MB controlled release system. Compared with traditional immobilization-based sensors, immobilization-free sensors (IFSs) avoid the modification of the recognition element on the electrode surface. Mesoporous SiO2 with large surface area and good biocompatibility can be used as nanocontainers to stably encapsulate the signal shuttle molecule methylene blue (MB). The bifunctional aptamer (APT) is used not only as the recognition element for ATZ but also as the signal switch to block or release MB. In the presence of ATZ, the specific recognition between ATZ and APT will cause the detachment of APT from the surface of SiO2, thus the molecular gate will open and release MB. Due to pH modulation, the positively charged MB can reach the surface of the negatively charged Ti(III) self-doped TiO2 NTs (Ti(III)–TiO2 NTs) electrode to act as an electron donor, which increases the photocurrent. The immobilization-free aptasensor has shown ultrasensitive detection of ATZ with a wide linear range from 1.0 pM to 100.0 nM and a low detection limit of 0.1 pM. In addition, the sensor has excellent selectivity, stability and anti-interference ability, and has been used in real water sample analysis successfully. This strategy has provided a new idea for the design of advanced immobilization-free PEC sensors for environmental pollutant detection.

Dual-luminophore fast-responding pressure-sensitive paint for the simultaneous elimination of motion- and temperature-induced errors

Abstract Motion- and temperature-induced errors are the major sources of error in pressure-sensitive paint (PSP) measurement. In this study, we developed a novel dual-luminophore fast-responding PSP with reference and pressure-sensitive channels that have similar temperature sensitivities, enabling motion- and temperature-induced errors to be simultaneously eliminated by taking the intensity ratio of the two channels. Rhodamine B (RhB), which was loaded on the Mobil Composition of Matter No. 41 (MCM-41) molecular sieve, and platinum tetrakis(pentafluorophenyl) porphyrin (PtTFPP) were chosen as the reference and pressure-sensitive luminophores, respectively. These luminophores were mixed with mesoporous SiO2 particles and a small amount of polymer to form a sprayable motion–temperature cancellation (MTC) PSP. By controlling the concentration of RhB, the temperature sensitivity of the reference channel was adjusted to match that of PtTFPP. To minimize temperature-induced errors, the effect of spectral ranges was also investigated. The lowest temperature sensitivity achieved for the MTC-PSP was 0.025%/℃, yielding an extremely low temperature-induced error of 55 Pa/℃. Its pressure sensitivity and response time were 0.46%/kPa and 145 μs, respectively. In addition, a theoretical model for the MTC-PSP that considers the effect of spectral overlap was proposed. The model accurately predicted the nonlinear relationship between the intensity ratio and pressure. The capability of the MTC-PSP was confirmed in a fast-rotating-disk experiment, and the pressure results agreed well with the theoretical pressure distribution on the disk.

Facile synthesis of SiOx/C as high-performance anodes for lithium-ion batteries

Despite its high capacity (∼2615 mAh·g−1), silicon oxide (SiOx) suffers from low electrical conductivity and volume expansion during cycling, leading to capacity fading. In this work, a SiOx/C composite was synthesized by magnesium thermal reduction using co-assembled mesoporous SiO2 and graphitic carbon nitride (g-C3N4) as precursors. The SiOx/C composite displays a sheet-like nanostructure with highly dispersed SiOx. The presence of carbon enhances the conductivity and structural stability of the composite, leading to a low charge resistance (94.0 Ω), fast lithium diffusion (DLi+ = 5.15 × 10−14 cm2·s−1), and excellent cycling capability (863 mAh·g−1 after 160 cycles).

Construction of sub micro-nano-structured silicon based anode for lithium-ion batteries

The significant volume change experienced by silicon (Si) anodes during lithiation/delithiation cycles often triggers mechanical-electrochemical failures, undermining their utility in high-energy-density lithium-ion batteries (LIBs). Herein, we propose a sub micro-nano-structured Si based material to address the persistent challenge of mechanic-electrochemical coupling issue during cycling. The mesoporous Si-based composite submicrospheres (M-Si/SiO2/CS) with a high Si/SiO2 content of 84.6 wt.% is prepared by magnesiothermic reduction of mesoporous SiO2 submicrospheres followed by carbon coating process. M-Si/SiO2/CS anode can maintain a high specific capacity of 740 mAh g−1 at 0.5 A g−1 after 100 cycles with a lower electrode thickness swelling rate of 63%, and exhibits a good long-term cycling stability of 570 mAh g−1 at 1 A g−1 after 250 cycles. This remarkable Li-storage performance can be attributed to the synergistic effects of the hierarchical structure and SiO2 frameworks. The spherical structure mitigates stress/strain caused by the lithiation/delithiation, while the internal mesopores provide buffer space for Si expansion and obviously shorten the diffusion path for electrolyte/ions. Additionally, the amorphous SiO2 matrix not only servers as support for structure stability, but also facilitates the rapid formation of a stable solid electrolyte interphase layer. This unique architecture offers a potential model for designing high-performance Si-based anode for LIBs.

“Template-assisted synthesis” strategy to improve the bifunctional catalytic activity of oxygen electrode for reversible protonic ceramic electrochemical cell

Reversible protonic ceramic electrochemical cell (R-PCEC) is a new energy conversion device that can achieve efficient conversion between chemical energy and electrical energy. However, the oxygen electrode has poor activity towards oxygen evolution reaction (OER) and oxygen reduction reaction (ORR), which hinders the large-scale application of R-PCEC. Therefore, in order to improve the catalytic activity of oxygen electrodes, La0.5Sr0.5Co0.2Fe0.8O3 (LSCF) is prepared using mesoporous silica SiO2 (SBA-15) and polymethyl methacrylate (PMMA) as templates, and it was combined with BaZr0.1Ce0.7Y0.2O3-δ (BZCY) to form a composite oxygen electrode for R-PCEC. Compared to the LSCF with solid-state reaction method, LSCF with hard-template method shows higher specific surface area and more oxygen vacancy, resulting in excellent ORR catalytic activity. In addition, LSCF prepared using PMMA as a template exhibits the best ORR catalytic activity, and the single cell with this composite oxygen electrode shows the highest maximum power density of 406 mW cm−2 in fuel cell (FC) mode with wet H2 (3%H2O) and air as the fuel and oxidant, respectively. Additionally, it demonstrates a current density of −973 mA cm−2 at 1.3 V in electrolysis cell (EC) mode with wet air (10%H2O) as the oxidant. The single cell also shows good reversibility in FC mode and EC mode. This work provides a new strategy to improve the bifunctional activity of oxygen electrode in R-PCEC.

Enhanced Cycling Stability of All-Solid-State Lithium-Sulfur Battery through Nonconductive Polar Hosts.

All-solid-state lithium-sulfur batteries (ASSLSBs) are promising next-generation battery technologies with a high energy density and excellent safety. Because of the insulating nature of sulfur/Li2S, conventional cathode designs focus on developing porous hosts with high electronic conductivities such as porous carbon. However, carbon hosts boost the decomposition of sulfide electrolytes and suffer from sulfur detachment due to their weak bonding with sulfur/Li2S, resulting in capacity decays. Herein, we propose a counterintuitive design concept of host materials in which nonconductive polar mesoporous hosts can enhance the cycling life of ASSLSBs through mitigating the decomposition of adjacent electrolytes and bonding sulfur/Li2S steadily to avoid detachment. By using a mesoporous SiO2 host filled with 70 wt % sulfur as the cathode, we demonstrate steady cycling in ASSLSBs with a capacity reversibility of 95.1% in the initial cycle and a discharge capacity of 1446 mAh/g after 500 cycles at C/5 based on the mass of sulfur.

CeO2 Nanoislands Enhance the Activity and Stability of Pt Nanoparticles Supported on Fibrous Mesoporous SiO2 for Catalytic Combustion of Propane

CeO₂ Nanoislands Enhance the Activity and Stability of Pt Nanoparticles Supported on Fibrous Mesoporous SiO₂ for Catalytic Combustion of Propane

Mesoporous silica encapsulated core-shell NiRh@NiO nanocatalyst for performance-enhanced ethanol steam reforming

A novel mesoporous SiO2 encapsulated core-shell NiRh@NiO nanostructure was constructed to enhance the activity and stability of ethanol steam reforming (ESR). At 550 °C and ethanol-WHSV = 21 h−1, the specific activity and conversion loss (17 h) of NiRh@NiO@m-SiO2 were 0.42 molethanol/(gcat.·h) and 10.9 %. The alloying of NiRh core, dominated NiO in shell, and abundant mesopore of SiO2 were confirmed by systematic characterization techniques. The in situ DRIFTS results indicated that alloying Ni and Rh boosted ethoxide dehydrogenation to acetaldehyde and acetate demethanation to carbonate. ReaxFF molecular dynamics (MD) simulations suggested that NiO shell was conducive to water activation, which, in turn, promoted the conversion of CHxOy species. The SiO2 encapsulation derived confinement effect inhibited both metal core sintering and leaching caused by the filamentous carbon. The abundant mesopores of SiO2 ensured the facile in-diffusion of water and out-diffusion of carbonaceous products, suppressing carbon deposition within the SiO2 encapsulation and the destruction of core-shell structure.

Preparation of highly dispersed Pt catalyst by CeOx 'islands' modification of dendritic mesoporous SiO2: CO as a probe molecule

There are still challenges in producing noble metal catalysts with atomic dispersion on a large scale in current. This study presents a method for preparing a highly dispersed Pt catalyst (Pt/CeOx/DMS) by modifying dendritic mesoporous SiO2 (DMS) with CeOx 'islands'. The results demonstrate that Pt is highly dispersed on the CeOx/DMS carrier, with the majority of Pt existing in the form of single atoms (Pt/20 wt.% CeOx/DMS). The dendritic DMS has a large specific area (451 m2 g−1) that provides more sites for the formation of CeOx 'islands'. This promotes the dispersion of Pt, significantly improving the oxidation activity of CO. The addition of CeOx 'islands' to the Pt/DMS catalyst promotes the desorption of CO on Pt NPs, enhances the adsorption and activation of O2, and further improves the activity of catalyst. This is because of the synergistic effect between Pt and CeOx 'islands'. The DFT result found that it is more beneficial for Pt1 to adsorb O2 before other reactions. To test the activity and stability of the catalyst, the team conducted catalytic oxidation of CO as a model reaction. The results show that the promotion of CeOx 'island' reduces T90 of CO catalytic oxidation by 16 ℃. In the high-temperature stability test of the catalyst, it was observed that even after reacting at 500 ℃ for 8 h, the catalyst can still achieve 90 % conversion of CO at 147 ℃, demonstrating its good stability. This study provides a foundation for designing a new type of CO oxidation catalyst. The catalyst features high dispersion Pt and exhibits excellent catalytic activity.

Innovative hydroxyapatite/submicron mesoporous SiO2/HA particles composite coatings for enhanced osteogenic activity of NiTi bone implants: A comprehensive investigation of materials and biological interactions

In this study, hydroxyapatite (HA) incorporated in mesoporous SiO2 particles (SiO2/HA) was initially synthesized using a soft template method. The resulting structure owned a high surface area (796.62 m2 g-1) and a substantial pore volume (0.757 cm3 g-1). The TEM and FESEM images indicated that the synthesized mesoporous particles possessed a spherical morphology, with a diameter of approximately 200 nm, and pores of slit-shape. The mission was to improve the surface characteristics of NiTi implants by composite coatings. This investigation emphasized specifically the impact of different concentrations of mesoporous SiO2/HA particles within the electrolyte on the properties of HA/mesoporous composite coatings. The surface characteristics of the coatings were examined using ATR-FTIR, FESEM, EDS, AFM, and hydrophilicity measurements. Furthermore, the osteogenic activity of the coatings was evaluated by culturing the osteosarcoma cell line SAOS-2 on the coated samples. The introduction of mesoporous particles resulted in a decrease in the coatings’ surface roughness along with an improvement in their wettability. Moreover, there was an enhancement in the cell viability (99% after 7 days), proliferation, adhesion, and growth of SAOS-2 cells at the optimized concentration of mesoporous SiO2/HA particles (1000 mg/L). DAPI images illustrated that the mesoporous particles did not cause any adverse impact on the cellular nuclei. Finally, in the apoptosis test, the composite coating displayed a reduced number of necrotic cells compared to the pure HA coating.

Efficient and reusable mesoporous silica structures for ciprofloxacin removal from water media

The present study aimed to evaluate the potential of mesoporous silica structures, synthesized by the sol-gel method, and using an inorganic precursor, in adsorbing the antibiotic ciprofloxacin (CIP) from water. The structures with the largest surface area and smallest pore size were selected for adsorption tests. Under optimal adsorption conditions, the results showed better correlation with the Langmuir isotherm for the non-porous SiO2 (SiO2–3) and with the Freundlich isotherm for the mesoporous SiO2 (M0.5-SiO2–3 and M1.5-SiO2–3). The kinetic parameters for all analyzed particles had a better fit to the pseudo second-order model. The highest adsorption capacity value obtained was 47.24 mg/g for M1.5-SiO2–3. The thermodynamic parameters indicated that the adsorption was spontaneous, endothermic, and mostly occurred through physical and multilayer adsorption mechanisms. By the analysis of intraparticle diffusion it is clear that a multistep adsorption process occurs, and therefore an adsorption mechanism was proposed. The influence of temperature, pH and presence of salt on the adsorption process was evaluated. The results show that increasing temperature favours adsorption, and that pH and the presence of ions directly influence the adsorption capacity of CIP, indicating that the major interactions controlling the process are mainly electrostatic. Reusability experiment was carried out for M1.5-SiO2–3, being possible to identify that after 5 adsorption-desorption cycles, the adsorbent still has significant CIP adsorption capacity. Based on the results the feasibility of synthesizing mesoporous structures, using a facile method, with high CIP adsorption capacity was demonstrated.

Pyrite-assisted degradation of methoxychlor by laccase immobilized on Fe3S4/earthworm-like mesoporous SiO2.

Laccase immobilized and cross-linked on Fe3S4/earthworm-like mesoporous SiO2 (Fe3S4/EW-mSiO2) was used to degrade methoxychlor (MXC) in aqueous environments. The effects of various parameters on the degradation of MXC were determined using free and immobilized laccase. Immobilization improved the thermal stability and reuse of laccase significantly. Under the conditions of pH 4.5, temperature 40 °C, and reaction time 8 h, the degradation rate of MXC by immobilized laccase reached a maximum value of 40.99% and remained at 1/3 of the original after six cycles. The excellent degradation performance of Fe3S4/EW-mSiO2 was attributable to the pyrite (FeS2) impurity in Fe3S4, which could act as an electron donor in reductive dehalogenation. Sulfide groups and Fe2+ reduced the activation energy of the system resulting in pyrite-assisted degradation of MXC. The degradation mechanism of MXC in aqueous environments by laccase immobilized on Fe3S4/EW-mSiO2 was determined via mass spectroscopy of the degradation products. This study is a new attempt to use pyrite to support immobilized laccase degradation.

Biomimetic Self-Propelled Asymmetric Nanomotors for Cascade-Targeted Treatment of Neurological Inflammation.

The precise targeted delivery of therapeutic agents to deep regions of the brain is crucial for the effective treatment of various neurological diseases. However, achieving this goal is challenging due to the presence of the blood‒brain barrier (BBB) and the complex anatomy of the brain. Here, a biomimetic self-propelled nanomotor with cascade targeting capacity is developed for the treatment of neurological inflammatory diseases. The self-propelled nanomotors are designed with biomimetic asymmetric structures with a mesoporous SiO2 head and multiple MnO2 tentacles. Macrophage membrane biomimetic modification endows nanomotors with inflammatory targeting and BBB penetration abilities The MnO2 agents catalyze the degradation of H2O2 into O2, not only by reducing brain inflammation but also by providing the driving force for deep brain penetration. Additionally, the mesoporous SiO2 head is loaded with curcumin, which actively regulates macrophage polarization from the M1 to the M2 phenotype. All in vitro cell, organoid model, and in vivo animal experiments confirmed the effectiveness of the biomimetic self-propelled nanomotors in precise targeting, deep brain penetration, anti-inflammatory, and nervous system function maintenance. Therefore, this study introduces a platform of biomimetic self-propelled nanomotors with inflammation targeting ability and active deep penetration for the treatment of neurological inflammation diseases.

Particle Size Measurement and Detection of Bound Proteins of Non-Porous/Mesoporous Silica Microspheres by Single-Particle Inductively Coupled Plasma Mass Spectrometry.

Single-particle inductively coupled plasma mass spectrometry (spICP-MS) has been used for particle size measurement of diverse types of individual nanoparticles and micrometer-sized carbon-based particles such as microplastics. However, its applicability to the measurement of micrometer-sized non-carbon-based particles such as silica (SiO2) particles is unclear. In this study, the applicability of spICP-MS to particle size measurement of non-porous/mesoporous SiO2 microspheres with a nominal diameter of 5.0 µm or smaller was investigated. Particle sizes of these microspheres were measured using both spICP-MS based on a conventional calibration approach using an ion standard solution and scanning electron microscopy as a reference technique, and the results were compared. The particle size distributions obtained using both techniques were in agreement within analytical uncertainty. The applicability of this technique to the detection of metal-containing protein-binding mesoporous SiO2 microspheres was also investigated. Bound iron (Fe)-containing proteins (i.e., lactoferrin and transferrin) of mesoporous SiO2 microspheres were detected using Fe as a presence marker for the proteins. Thus, spICP-MS is applicable to the particle size measurement of large-sized and non-porous/mesoporous SiO2 microspheres. It has considerable potential for element-based detection and qualification of bound proteins of mesoporous SiO2 microspheres in a variety of applications.

Preparation of surface molecular imprinting fluorescent sensor based on magnetic porous silica for sensitive and selective determination of catechol.

A magnetic fluorescent molecularly imprinted sensor was successfully prepared and implemented to determine catechol (CT). Fe3O4 nanoparticles were synthesized by the solvothermal technique and mesoporous Fe3O4@SiO2@mSiO2 imprinted carriers were prepared by coating nonporous and mesoporous SiO2 shells on the surface of the Fe3O4 subsequently. The magnetic surface molecularly imprinted fluorescent sensor was created after the magnetic mesoporous carriers were modified with γ-methacryloxyl propyl trimethoxy silane to introduce double bonds on thesurface of the carries and the polymerization was carried out in the presence of CT and fluorescent monomers. The magnetic mesoporous carriers were modified with γ-methacryloxyl propyl trimethoxy silane and double bonds were introduced on the surface of the carriers. After CT binding with the molecularly imprinted polymers (MIPs), the fluorescent intensity of the molecularly imprinted polymers (Ex = 400nm, Em = 523nm) increased significantly. The fluorescent intensity ratio (F/F0) of the sensor demonstrated a favorable linear correlation with the concentration of CT between 5 and 50μM with a detection limit of 0.025μM. Furthermore, the sensor was successfully applied to determine CT in actual samples with recoveries of 96.4-105% and relative standard deviations were lower than 3.5%. The results indicated that the research of our present work provided an efficient approach for swiftly and accurately determining organic pollutant in water.

A smartphone-assisted fluorescent sensing platform for ochratoxin A using Mn-doped CsPbBr3 perovskite quantum dots embedded in the mesoporous silica as a ratiometric probe

Food sources are susceptible to contamination with ochratoxin A (OTA), which is a serious threat to human health. Thus, the construction of novel, simple sensing platforms for OTA monitoring is of utmost need. Manganese-doped lead halide perovskite quantum dots encapsulated with mesoporous SiO2 (Mn-CsPbBr3 QDs@SiO2) were prepared here and used as a ratiometric fluorescent probe for OTA. Mn-CsPbBr3 QDs, synthesized at room temperature, exhibit dual emission with maximum wavelengths of 440 and 570 nm and, when embedded in the SiO2 layer, produce a stable and robust photoluminescence signal. By adding OTA to the probe, emission at 440 nm increases while emission at 570 nm decreases, so a ratiometric response is obtained. Experimental variables affecting the probe signal were studied and optimized and the mechanism of sensing was discussed. This ratiometric sensor demonstrated excellent selectivity and low detection limit (4.1 ng/ml) as well as a wide linear range from 5.0 to 250 ng/ml for OTA. A simple portable smartphone-based device was also constructed and applied for the fluorescence assay. With different OTA concentrations, the multicolor transition from pink to blue under a UV lamp led to simple visual and smartphone-assisted sensing of OTA by using a color analyzing application. Satisfactory recoveries in black tea, coffee, moldy fig and flour samples confirmed the reliability of the assay. The accuracy of the probe was proved by comparison of the results with high-performance liquid chromatography (HPLC).

Hierarchical Dendritic Photonic Crystal Beads for Efficient Isolation and Proteomic Analysis of Multiple Cell Types.

Cell types with different morphology, and function collaborate to maintain organ function. As such, analyzing proteomic differences and connections between different types of cells forms the foundation for establishing functional connectomes and developing in vitro organoid simulation experiments. However, the efficiency of cell type isolation from organs is limited by time, equipment, and cost. Here, hierarchical dendritic photonic crystal beads (HDPCBs) featuring high-density functional groups via the self-assembly of dendritic mesoporous structure SiO2 nanoparticles (DM-SiO2) and grafting dendrimers onto the surface of dendritic mesoporous photonic crystal beads (DMPCBs) is developed. This platform integrates multitype cell separation with in situ protein cleavage processes. Efficient simultaneous isolation of Kupffer cells and Liver Sinusoidal Endothelial cells (LSECs) from liver, with high specificity and convenient operation in a short separation time are demonstrated. The results reveal 2832 and 3442 unique proteins identified in Kupffer cells and LSECs using only 50 HDPCBs, respectively. 764 and 629 over-expressed proteins associated with the function of Kupffer cells and LSECs are found, respectively. The work offers a new method for efficiently isolating multiple cell types from tissues and downstream proteomic analysis, ultimately facilitating the identification of primary cell compositions and functions.