Mesocellular Foam Research Articles

Real-Time Imaging of Cell Therapy with Acoustics

Background & Aim Cell therapy has shown significant promise in treating disease. Unfortunately, stem cell therapy is limited by mis-injection into highly fibrotic tissues, poor cell survival due to ischemia and inflammation, and low cell retention. For these reasons, our group has developed a variety of tools that facilitae real-time imaging of stem cell therapy. We are particualrly intersted in acoustic and photoacoustic imaging because of their low cost, ease of use, and video frame rates to faciliate real-time imaging of the cell delivery event as well as downstream analysis of cell biodistribution. We have built several novel nanoparticles contrast agents and used them image mesenchymal stem cell delivery and location in vivo. These same nanoparticles can often also provide MRI signal or deliver pro-survival drugs in addition to reporting the location of the therapeutic cells. Methods, Results & Conclusion Here, I will discuss the value of acoustic imaging as applied to cell therapy using cardiac cell therapy as an example. This work used a biocompatible multi-functional silica-iron oxide nanoparticle (SIO) made via an in situ growth of Fe3O4 nanoparticles on both the external surfaces and pore walls of mesocellular foam silica nanoparticles. In contrast to prior work, this approach builds a magnetic moiety inside the pores of a porous silica structure. These materials serve three roles: drug delivery, magnetic manipulation, and imaging. The addition of Fe3O4 to the silica nanoparticles increased their colloidal stability, T2-based magnetic resonance imaging (MRI) contrast, and superparamagnetism. The particle core offered acousticv signal. We then used the hybrid materials as a sustained release vehicle of insulin-like growth factor—a pro-survival agent that can increase cell viability. In vivo rodent studies show that labeling stem cells with this nanoparticle increased the efficacy of stem cell therapy in a ligation/reperfusion model. The nanoparticle-labeled cells increase the mean left ventricular ejection fraction by 11% and 21% and the global longitudinal strain by 24% and 34% on days 30 and 60, respectively. In summary, this multifunctional nanomedicine improves stem cell survival via the sustained release of pro-survival agents.

Characterization of Mesoporous Silica and its Application as an Adsorbent for Benzene Sensing

Mesoporous silica (MPS) is a porous silica material with various pore structures. In this study, mesocellular foam silica (MCF) was synthesized and functionalized by hexamethyldisilazane (HMDS) to study effects of surface chemistry on benzene adsorption capability. Physical and chemical properties of pristine and functionalized MCFs were characterized and compared. Scanning and transmission electron microscopy showed that the complex pore structures of the MCFs were retained after the functionalization at relatively high temperature (573K). TGA and FTIR results showed that the functionalization led to a reduction of water adsorbed on the surfaces of the MCF. The functionalization improved adsorption of benzene compared to the pristine MCF and the optimum HMDS:SiO2 molar ratio was 1.5. The amount of benzene adsorbed has a linear relationship with the concentration of benzene in the environment. This relationship enables quantitative benzene detection by using the functionalized MCF as sensing materials in resistive-type or gravimetric-type benzene gas sensors.

Pyridine-, thiol- and amine-functionalized mesoporous silicas for adsorptive removal of pharmaceuticals

Abstract Pyridine-, thiol- and amine-functionalized mesoporous silica sorbents have been synthesized by co-condensation of tetraethoxysilane (TEOS) with the proper organosilica monomers. The resulting structural and morphological features were controlled not only by the arrangement of the polymeric template but also by the type of the functional group. Amine- and thiol-functionalized silicas were composed of aggregated nanoporous rings/tubes while pyridine-functionalized silica formed meso-cellular foam (MCF). Studies on adsorptive removal of pharmaceuticals showed that the fabricated amine- and pyridine-functionalized silicas adsorbed high amounts of pharmaceuticals within minutes. A gradual dissolution of loosely aggregated silica nanorings of aminated and thiolated silicas was observed, while the compact MCF structure of pyridine-functionalized sorbent was more resistant to water-driven degradation. As a result, the latter material could be reused without significant deterioration of its adsorption efficiency, which shows its usefulness for the removal of hazardous pollutants including pharmaceutical from waters and wastewaters.

Evaluation of eosin Y removal from aqueous solution using nano-mesoporous material MCFs: adsorption equilibrium, kinetics, and adsorption isotherms

Mesocellular foams (MCFs) silica was successfully synthesized using hydrothermal method. The mesoporous material was characterized by X-ray diffraction, infrared spectroscopy, low temperature nitrogen adsorption–desorption, scanning electron microscopy, transmission electron microscopy. The low temperature nitrogen adsorption–desorption results showed that the synthesized MCFs has a diameter of 12 nm. Transmission electron microscopy revealed that the MCFs synthesized had a good honeycomb structure aperture and is conducive to the adsorption of dye macromolecule. This paper carried out the study on the adsorption of eosin Y by MCFs. The adsorption conditions of eosin Y by MCFs were optimized and the optimum adsorption conditions obtained were: MCFs:(eosin Y) = 250:1, pH 2.0, contact time 10 min at a room temperature of 22 ± 1 °C. Under the conditions, the adsorption effect was the best, the adsorption rate reached 97.95% and the adsorption capacity reached 3.96 mg/g. The research results of adsorption kinetics for the adsorption system displayed that the adsorption is the pseudo-second-order adsorption. The research results of adsorption thermodynamics showed that △G0 < 0, ΔH0 = − 40.08 kJ/mol, ΔS0 = − 51.11 J/(mol K), and the adsorption is an exothermic, spontaneous and entropy reduction reaction process. This adsorption conforms to the isothermal adsorption equation of Langmuir, belonging to a single molecular layer adsorption. All the linear correlation coefficients of the Langmuir isotherm equations fitting (R2) were greater than 0.999.

Synthesis, characteristics and application of mesocellular foam carbon (MCF-C) as catalyst for dehydrogenation of ethanol to acetaldehyde

This research focused on synthesis of mesocellular foam carbon (MCF-C) and this catalyst was employed dehydrogenation of ethanol to acetaldehyde. The mesocellular foam silica (MCF-Si) was used as the template of material following by converting the surfactant residue (Pluronic P123) into carbon layers using H2SO4 and NaOH etching. The obtained MCF-C exhibited the highest surface area of 995 m2/g among these materials, and mesoporous size of 4.2 nm with spherical shape and interconnected pore. Furthermore, total acidity of MCF-C also increased from 427.07 (MCF-Si) to 682.64 μmole. In the part of catalytic test, the MCF-C was used in gas-phase ethanol dehydrogenation at 200–400 °C. It revealed that the MCF-C exhibited the highest ethanol conversion (ca. 17.5 %) at 400 °C due to its high acidity without significant deactivation of catalyst within 12 h. Besides, its high ethanol conversion, it is worth noting that the acetaldehyde selectivity (ca. 80.3 %) was also high, especially at 400 °C. This can be attributed to the proper mesoporous size that can facilitate the diffusion of acetaldehyde without further decomposing at high temperature.

Surface Plasmon Resonance Sensing Performance and Adsorption Law of Self-Assembly Glucose-Sensitive Membrane

A high-sensitive surface plasmon resonance (SPR) sensor was proposed using a self-assembly optical glucose-sensitive membrane (OGSM) and an osmotic-protection membrane on a glass/gold sheet. A prism and an OGSM were assembled to form a multilayer structure such as glass/gold/(PDDA/PSS)2/(PDDA/ImGODs)n/(PVA+PEG), where the ImGODs layer refers to immobilized glucose oxidases (ImGODs) on mixed nanoparticles consisting of SiO2 nanoparticles (SiNPs) and mesocellular foams (SiMCFs) and where polyvinyl alcohol and polyethylene glycol were used to form an osmotic-protection film. Many glucose-sensing experiments were performed to determine the optimal parameters of the OGSM proposed. For the OGSM with a mass ratio of SiMCFs:SiNPs = 7:3, the plasmon resonance angle of the glucose sensor reduced 2.60° and 0.26°/(mg/dL) of average sensitivity within the glucose concentration range from 0 to 10 mg/dL. The relationships among the resonance angle shift of the sensor, refractive index of the OGSM, and adsorption isotherm of the OGSM were established. The relationships showed that the Langmuir isotherm model was suitable for describing the adsorption process of the OGSM for glucose molecules in the concentration range of 0–80 mg/dL. For the SPR glucose sensor with three sensitive bilayers (PDDA/ImGODs)3, the dependence relationships of resonance angle shift and sensor sensitivity on glucose concentration, $\Delta \theta _{\text {res}}=-{2.076} {C}$ / ( ${1}+{0.707} {C}$ ) and ${S} = {2.076}$ /( ${1}+{0.707} {C}$ )2, were obtained.

Design and synthesis of a versatile cooperative catalytic aerobic oxidation system with co-immobilization of palladium nanoparticles and laccase into the cavities of MCF

We have designed a versatile reusable cooperative catalyst oxidation system, consisting of palladium nanoparticles and laccase with unprecedented reactivity. This biohybrid catalyst was synthesized by the stepwise immobilization of laccase as an enzyme and Pd as a nanometallic component into the same cavity of siliceous mesocellular foams (MCF). MCF and nanobiohybrid catalyst were characterized by BET, SAXS, SEM, EDX elemental mapping, ICP-OES, TEM, TGA, FT-IR, and XPS techniques and the stepwise immobilization of laccase enzyme and Pd onto MCF was evaluated through several compelling electrochemical studies. The present catalytic system exhibits high activity toward (i) aerobic oxidation of alcohols to the corresponding carbonyl compounds, (ii) aerobic oxidation of cyclohexanol and cyclohexanone to phenol and (iii) aerobic dehydrogenation of important N-heteocyclic compounds (tetrahydro quinazolines, quinazolonones, pyrazolines and 1,4-diydropyridines) in the presence of catalytic amount of hydroquinone (HQ) as mediator in phosphate buffer (0.1 M, pH 4.5, 4 mL)/THF (4%, 1 mL) as solvent under mild conditions. The immobilization of both oxygen-activating catalyst (laccase) and oxidizing catalyst (Pd) onto the same support makes the present catalyst system superior to other currently available heterogeneous palladium based catalytic aerobic oxidation systems.

Pd nanoparticles confined in mesoporous N-doped carbon silica supports: a synergistic effect between catalyst and support

Palladium nanoparticles of similar size were deposited on different supports, layers of carbon materials (with and without nitrogen doping) on the surface of a MCF (mesocellular foam) silica.

A Refractometric Uric Acid Biosensor Based on Immobilized Uricase and PVA+PEG Composite Hydrogels

A mechanically stable, high-sensitivity, high-selectivity, optical uric acid sensitive membrane (UASM) was prepared by immobilizing uricase in a mixture of SiO2 mesocellular foams (SiMCFs) and nanoparticles (SiNPs), then embedding it in a composite gel of polyvinyl alcohol (PVA) and polyethylene glycol (PEG). The UASM was spin-coated onto a gold-glass sheet to create a surface plasmon resonance (SPR) biosensor without electromagnetic and light-intensity disturbances. A series of prism-based SPR sensing experiments showed that, for a UASM consisting of SiMCFs and SiNPs at a mass ratio of 6:4, PVA and PEG at a mass ratio of 4:1, the resonance angle of the sensor decreased by about 2.610° and the average sensitivity was 2.175°/mM within a UA concentration range of 0 – 1.2 mM. Young’s moduli of four dry UASM strips (670 – 1050 MPa) were obtained, demonstrating that UASMs have good biomechanical stability. Langmuir adsorption isotherm could explain the adsorption law of the UASMs for UA molecules in the concentration range of 0.2 – 1.2 mM.

Understanding of the formation of mesocellular-like silica foam particles of nano size and its chemical surface to immobilization of Thermomyces lanuginosus lipase

Abstract Mesocellular foam (MCF)-like silica particles were produced from a nanometric mesoporous silica material with unique characteristics. Conditions during synthesis play a crucial role in the porous properties and chemical surface of MCF silica. In this work, the effects of various factors, including the H+/silica source molar ratio, the presence of a mineralizing agent, synthesis temperature, and the presence of combined surfactants (e.g., P123 and CTAB), on the porous properties of MCF silica were assessed. Additionally, the surface of these particles was modified with amine groups to evaluate performance as support for enzymatic immobilization. Thermomyces lanuginosus lipase was immobilized on the surfaces of nanoparticles functionalized with amine groups at pH 7 and 25 °C. Under these conditions, the enzyme had a net negative charge (pI = 4.4), while the functionalized particles had a net positive charge, which promotes enzymatic interactions.

Nano mesocellular foam silica (MCFs): An effective adsorbent for removing Ni2+ from aqueous solution

Nano mesocellular foam silica (MCFs) was synthesized through the hydrothermal method in this study. Powder X-ray diffraction and scanning electron microscopy were used to characterize the MCFs sample. The sample presented spherical particles and regular morphology. The results of transmission electron microscopy showed that synthesized MCFs has a three-dimensional honeycomb pore structure, which aids in the adsorption of nickel ion (Ni2+). The results of low-temperature nitrogen gas adsorption-desorption showed that the pore diameter of the synthesized MCFs was 19.6 nm. The impacts of pH, temperature, amount of adsorbent, initial concentration of Ni2+, and contact time on the adsorption effect of Ni2+ by MCFs were studied. Under the optimized adsorption conditions, the adsorption rate reached 96.10% and the adsorption capacity was 7.69 mg/g. It has been determined through the study of kinetics and adsorption isotherms that the adsorption of Ni2+ by MCFs follows the pattern of the pseudo-second-order kinetic model, simultaneously belonging to the Freundlich adsorption type. The thermodynamic results of adsorption showed that, when the temperature is between 25°C and 45°C, the adsorption is a spontaneous exothermic reaction.

Multi-functional Fe-Al0.66DTP/MCF catalyst in cascade engineered synthesis of the drug butamben: Novelty of catalyst, reaction kinetics and mechanism

Cascade engineered synthesis of the drug butamben (n-butyl-p-aminobenzoate), a local anaesthetic, which is used for the treatment of chronic pain, is presented here. A new multifunctional heterogeneous catalyst was synthesized, characterized and used for the direct synthesis of n-butyl-p-aminobenzoate by hydrogenation and esterification. A mixture of p-nitrobenzoic acid, n-butanol, hydrogen, acetonitrile as solvent and catalyst was used and hydrogenated in which hydrogenation precedes esterification. A series of aluminum exchanged dodecatungstophosphoric acids (DTP) (Alx-DTP; x = 0.33–1) (5–20% w/w) supported on mesocellular foam silica (MCF) were prepared. 2% Fe loaded on 15% Al0.66DTP/MCF showed the best activity for one-pot synthesis because the metal and acid sites had proper strength and distribution on MCF. Reaction mechanism and kinetic model were developed. The catalyst 2%Fe-15% Al0.66DTP/MCF was found reusable for four catalytic cycles. This work presents an interesting case of reaction engineering using multi-functional catalyst useful in synthesis of a pharmaceutical drug butamben and has potential applications in other areas.

Targeted therapeutic effect against the breast cancer cell line MCF-7 with a CuFe2O4/silica/cisplatin nanocomposite formulation.

The combination of magnetic nanoparticles with a porous silica is a composite that has attracted significant attention for potential multifunctional theranostic applications. In this study, 30 wt % CuFe2O4 was impregnated into a matrix of monodispersed spherical hydrophilic silica (HYPS) nanoparticles through a simple dry impregnation technique. The chemotherapy drug cisplatin was loaded through electrostatic equilibrium adsorption over 24 h in normal saline solution. The presence of cubic spinel CuFe2O4 on HYPS was confirmed through powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR) and diffuse reflectance UV–vis spectroscopy (DR UV–vis) analysis. The HYPS particles showed a surface area of 170 m2/g, pore size of 8.3 nm and pore volume of 0.35 cm3/g. The cisplatin/CuFe2O4/HYPS nanoformulation showed the accumulation of copper ferrite nanoparticles on the surface and in the pores of HYPS with a surface area of 45 m2/g, pore size of 16 nm and pore volume of 0.18 cm3/g. Transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) mapping analysis showed the presence of homogeneous silica particles with nanoclusters of copper ferrite distributed on the HYPS support. Vibrating sample magnetometry (VSM) analysis of CuFe2O4/HYPS showed paramagnetic behavior with a saturated magnetization value of 7.65 emu/g. DRS UV–vis analysis revealed the functionalization of cisplatin in tetrahedral and octahedral coordination in the CuFe2O4/HYPS composite. Compared to other supports such as mesocellular foam and silicalite, the release of cisplatin using the dialysis membrane technique was found to be superior when CuFe2O4/HYPS was applied as the support. An in vitro experiment was conducted to determine the potential of CuFe2O4/HYPS as an anticancer agent against the human breast cancer cell line MCF-7. The results show that the nanoparticle formulation can effectively target cancerous cells and could be an effective tumor imaging guide and drug delivery system.

SPIONs/3D SiSBA-16 based Multifunctional Nanoformulation for target specific cisplatin release in colon and cervical cancer cell lines

Multifunctional nanomaterials can be used for dual applications: drug delivery as well as in bioimaging. In current study, we investigated potential use of silica based supports; 3D cage type SiSBA-16 (S-16), monodispersed hydrophilic spherical silica (HYPS) and mesocellular foam (MSU-F) for cisplatin (Cp) delivery. To obtain magnetic resonance characteristics, 10 wt% iron oxide was loaded through enforced adsorption technique. For pH stimuli responsive release of Cp, 10 wt%SPIONs/S-16 was functionalized with 3-(Aminopropyl)triethoxysilane (A) and poly acrylic acid (PAA) termed as 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp. By TEM analysis, the average diameter of the SPIONs was found to range between 10–60 nm. VSM analysis showed saturation magnetization over S-16, HYPS and MSU-F were in the following order: 10 wt%SPIONs/HYPS (4.08 emug−1) > 10 wt%SPIONs /S-16 (2.39 emug−1) > 10 wt%SPIONs/MSU-F (0.23 emug−1). Cp release study using dialysis membrane in PBS solution over 10 wt%SPIONs/S-16 nanoformulations showed highest cumulative release (65%) than 10 wt%SPIONs/MSU-F-A-Cp (63%), 10 wt%SPIONs/HYPS-A-Cp (58%), and Cp-F127/S-16 (53%), respectively. 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp were evaluated for in vitro target anticancer efficiency in human cancer cell lines (colon cancer (HCT 116), cervical cancer (HeLa)) and normal cells (Human embryonic kidney cells (HEK293) using MTT and DAPI staining. 10 wt%SPIONs/S-16-A-Cp treated Hela and HCT116 cancerous cell lines showed significant control of cell growth, apoptotic activity and less cytotoxic effect as compared to Cp and 10 wt%SPIONs/S-16. Target specific Cp release in the cells shows that 10 wt%SPIONs/S-16-A-Cp can be easily upgraded for magnetic resonance imaging capability.

Functionalized mesoporous silica as matrix for shape-stabilized phase change materials

Shape-stabilized phase change materials (ssPCM) are used to reversibly store thermal energy while keeping their macroscopic solid shape. A porous matrix, such as mesoporous silica, can be used to obtain ssPCM through impregnation. However, a non-melting interface layer is formed between the matrix and heat storage component, which severely reduces the available pore volume and the energy storage capacity of the composite. We studied functionalization of mesoporous silica as a strategy for engineering the non-melting layer and improving the heat storage of the resulting composites. Stearic acid was used as the active heat storage phase and mesocellular foam silica as the porous matrix. Phenyl and methyl groups were found to increase the nanoconfined phase enthalpy. The non-melting layer volume is similar to unfunctionalized silica, suggesting that methyl and phenyl groups are present inside the non-melting layer. Carboxylic acid functionalization decreases the layer volume, yielding the highest enthalpy of the nanoconfined phase, of 34.5 J/g. This value is 15% higher than the nanoconfined enthalpy of materials containing unfunctionalized silica. Total heat storage values of 130 J/g were obtained for PCM samples containing phenyl or carboxylic acid functionalized matrices. The functionalized materials show high stearic acid loading (72–79 wt%), shape-stability and constant thermal response over 200 heating-cooling cycles. A facile method for assessing the non-melting layer volume is provided. The study shows that suitable surface functionalization can suppress the non-melting layer formation, providing a path for enhancing the heat storage potential of shape-stabilized PCM containing mesoporous silica matrices.

Formation lipase cross-linked enzyme aggregates on octyl-modified mesocellular foams with oxidized sodium alginate

Supported cross-linked enzyme aggregates were prepared by immobilization of Candida antarctica lipase B onto hydrophobic surface of octyl-modified mesocellular foams (MCFs-C8). Oxidized sodium alginate was used as a substitute for traditional glutaraldehyde. Supported cross-linked enzyme aggregates using oxidized sodium alginate (SA-CLEAs@MCFs-C8) exhibited significantly improved thermal stability and organic solvents tolerance compared to the free lipase, lipase adsorbed onto MCFs-C8 and supported cross-linked enzyme aggregates using glutaraldehyde (G-CLEAs@MCFs-C8). Then immobilized lipases were employed for biodiesel production by transesterification of soybean oil with methanol. In the optimization condition, SA-CLEAs@MCFs-C8 were quite stable and still showed high fatty acid methyl esters (FAME) yield after 5 repeated cycles (from 89% to 78%), whereas MCFs-C8-CALB retained 67% FAME yield (about 72% for G-CLEAs@MCFs-C8).

Enhancing Activity by Supercritical CO2 Mediated Immobilization of Lipase on Mesocellular Foam in Preparation of Hexyl Laurate.

Hexyl laurate is employed in several cosmetics having great demand. It could be synthesized catalytically like a "natural" perfume using a lipase. The use of mesocellular foam silica (MCF) for immobilization of lipases could be made using supercritical CO2 as a medium to enhance its activity in comparison with the normal techniques. Three different catalysts were supported on MCF such as Candida antractica B (CALB), Amano AYS, and Porcine pancreas (PPL), and their activity was evaluated in the preparation of hexyl laurate from lauric acid and hexyl alcohol. CALB@ MCF was the best among all. A systematic study was conducted to assess the effects of different operating parameters. It was ternary complex mechanism with inhibition by hexyl alcohol. The enzyme was reusable and the process is green.

Technoeconomic Evaluation of a Process Capturing CO2 Directly from Air

Capturing CO2 directly from air is one of the options for mitigating the effects global climate change, and therefore determining its cost is of great interest. A process model was proposed and validated using laboratory results for adsorption/desorption of CO2, with a branched polyethyleneimine (PEI) loaded mesocellular foam (MCF) silica sorbent. The model was subjected to a Multi-Objective Optimization (MOO) to evaluate the technoeconomic feasibility of the process and to identify the operating conditions which yielded the lowest cost. The objectives of the MOO were to minimize the cost of CO2 capture based on a discounted cash flow analysis, while simultaneously maximizing the quantity of CO2 captured. This optimization identified the minimum cost of capture as 612 USD tonne−1 for dry air entering the process at 25 °C, and 657 USD tonne−1 for air at 22 °C and 39% relative humidity. The latter represents more realistic conditions which can be expected for subtropical climates. The cost of direct air capture could be reduced by ~42% if waste heat was utilized for the process, and by ~27% if the kinetics of the sorbent could be improved by a factor of two. A combination of both would allow cost reductions of ~54%.

Development of novel support for penicillin acylase and its application in 6-aminopenicillanic acid production

There is an ever-increasing demand for the β-lactam bulk intermediate 6-aminopenicillanic acid (6-APA) that has wide applications in the synthesis of newer generations of semisynthetic penicillins. It is commercially synthesized by biocatalytic transformation using penicillin acylase. Since the enzyme is soluble, immobilization on a solid porous support is necessary to make the catalyst recycleable and the process profitable. In this study, we developed a novel support of siliceous foam entrapped in a polymer matrix. Penicillin acylase was covalently immobilized on aminopropyl functionalized mesocellular foam silica (MCF) and was further cross-linked using glutaraldehyde without deactivation and upto 95% efficiency. The resulting biocatalyst had an activity of 1185 IU. mg−1 and demonstrated improved resistance to the substrate and product inhibition. These parameters along with improvement in pH and thermal stability enhanced 6-APA yield by 20% in beads. Intrinsic kinetic parameters were calculated from the developed rate equation to deduce enzyme catalytic mechanism.

Study on the immobilization of hemoglobin by nano mesoporous MCFs and its optical properties

Nano mesoporous molecular sieve MCFs (mesocellular foams) was synthesized successfully through hydrothermal method. Then bovine hemoglobin was successfully immobilized into the mesoporous molecular sieve MCFs, the immobilized enzyme was 50.27 mg g−1. The desirable adsorbate/adsorbent, Hb/MCFs, (mass ratio, m/m) for 3/50 was achieved by adjusting adsorption conditions. The obtained materials were characterized using various techniques such as the powder XRD, Fourier transform infrared spectroscopy, UV–Vis solid diffuse reflectance spectroscopy (SDRS), photoluminescence spectrum and N2 adsorption desorption study at 77 K. The results showed that hemoglobin had been immobilized successfully inside the channels of mesoporous MCFs. Scanning electron microscopy was used to confirm the average particle diameters of MCFs-Hb and (CH3-MCFs)-Hb. And the results showed that the average particle diameters of MCFs-Hb and (CH3-MCFs)-Hb were 2212 ± 50 nm and 2219 ± 50 nm, respectively. The adsorption process of MCFs/(CH3-MCFs) adsorbing hemoglobin enzyme was in accordance with pseudo-second-order kinetic equation. The process of MCFs/(CH3-MCFs) adsorbing hemoglobin enzyme belonged to an exothermic reaction which was a spontaneous reaction. Freundlich model described the adsorption of hemoglobin by MCFs/CH3-MCFs well. The desorption process of MCFs-Hb/(CH3-MCFs)-Hb composite was studied in the sodium hydroxide solution (0.1 mol L−1), and equilibrium was reached at 5 h. The desorption rates were 72.58% and 69.67%, respectively. The luminescence spectra showed that the obtained composites had the light-emitting properties.