Event Abstract Back to Event Identification of PINK1 in the brain, eye and ear of mouse during embryonic development Marta D'Amora1*, Manuela Marcoli2, Cristiano Angelini1, Alberta Mandich1, Chiara Cervetto2 and Mauro Vallarino1 1 University of Genoa, Department of Biology, Italy 2 University of Genoa, Department of Experimental Medicine, Italy PINK1 is a 581 amino acid protein with a serine/threonine kinase domain and an N-terminal mitochondrial targeting motif. The enzyme is expressed in the brain as well as in several tissues such as heart, skeletal muscle, liver, kidney, pancreas and testis. In the present study, we have investigated by Western blot analysis and immunohistochemistry the presence and distribution of PINK1 in the brain, eye and inner ear of mouse during embryonic development. In the brain we detected a PINK1 molecular form of 66 kDa. Immunoreactive perikarya first appeared at stage E15 in the diencephalon within the thalamus, the hypothalamus, the periventricular layers of the third ventricle and in the rhombencephalon at level of the pons. Subsequently, new PINK1-positive neurons were found in the midbrain within the floor and the periventricular layers of the ventral wall of the mesencephalic vesicle (stage E17) as well as in the neopallial cortex, the tegmentum of the midbrain and the periventricular region of the caudal part of the rhombencephalon (stage E19). At P0, PINK1¬immunoreactive cells appeared in the striatum, the mantle layer and caudal part of the medulla oblongata and the cerebellum. The spatio-temporal expression of PINK1 and its heterogeneous distribution suggest that the enzyme might be involved in neuroregulatory processes during embryogenesis. In the eye, PINK1-immunoreactivity was found in lens and in the cornea, whereas in the inner ear the enzyme was expressed in the ependymal and subependymal cells of the saccule and in the semicircular canals indicating that PINK1 plays a role in the development of these sensory organs. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: D'Amora M, Marcoli M, Angelini C, Mandich A, Cervetto C and Vallarino M (2010). Identification of PINK1 in the brain, eye and ear of mouse during embryonic development. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00072 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Marta D'Amora, University of Genoa, Department of Biology, Genoa, Italy, marta.damora@iit.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marta D'Amora Manuela Marcoli Cristiano Angelini Alberta Mandich Chiara Cervetto Mauro Vallarino Google Marta D'Amora Manuela Marcoli Cristiano Angelini Alberta Mandich Chiara Cervetto Mauro Vallarino Google Scholar Marta D'Amora Manuela Marcoli Cristiano Angelini Alberta Mandich Chiara Cervetto Mauro Vallarino PubMed Marta D'Amora Manuela Marcoli Cristiano Angelini Alberta Mandich Chiara Cervetto Mauro Vallarino Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.