Eight new species of Ramalina are described from Venezuela: R. andina V. Marcano & A. Morales, R. chiguarensis V. Marcano & A. Morales, R. crispata A. Morales & V. Marcano, R. lopezii A. Morales & V. Marcano, R. microphylla A. Morales & V. Marcano, R. santanensis A. Morales & V. Marcano, R. tenuissima V. Marcano & A. Morales, and R. tovarensis V. Marcano & A. Morales. The genus Ramalina Ach. is represented in Venezuela by over 30 described species. Ramalina spp. are present in most vegetation types, including lowland, highlands (tepuy), heathlands and woodlands, rain forests, and alpine moorlands (paramos), and occur on rocks, peaty soil, wood, or bark. During a taxonomic revision of Venezuelan members of Ramalina we encountered several undescribed species, eight of which are described below. They are very distinctive species and have been collected only recently in poorly known areas, such as the Peninsula de Paraguana in the State of Falc6n, and in the Andes, both of which possess a high diversity of lichens. MATERIALS AND METHODS The species described below are based on material collected by Antonio Morales, Lopez-Figueiras, and Vicente Marcano in Venezuela in the states of Falc6n, Merida, Taichira, and Trujillo. The specimens cited in this paper are deposited in MERF and VEN. Chemical data were obtained by thin-layer chromatography (TLC) by means of standard methods (White & James 1985) and by isolation with petroleum ether, benzene, and acetone of chemical compounds for comparison from Ramalina usnea (L.) Howe, R. pusiola Muill. Arg., and Cladonia cryptochlorophaea Asah. The substances isolated were usnic, salazinic, stictic, protocetraric, divaricatic, and cryptochlorophaeic acids. These compounds were identified by comparison of their physical and spectroscopical (IR) properties with reports in the chemical literature (Culberson 1969; Culberson et al. 1977). In the case of the mixture sekicaic and homosekicaic acids the compounds were identified by discrimination of the 1HNMR and 13C-NMR signals. The employed instruments were: Jeol GSX 270 FT-NMR and VARIAN FT-80-A (Morales & Marcano 1992). Freezing microtome sections ofthalli and apothecia were mounted in lactophenol/cotton blue and examined by light microscopy (LM). THE SPECIES RAMALINA ANDINA V. Marcano & A. Morales sp. nov. (FIG. 1-2) Thallus corticolus, pendulus; laciniae subnitidae, complanatae vel canaliculatae; pseudocyphelliis ellipsoideis instructis; soralia lateralia vel laminalia; pycnidia non visa; apothecia non visa; acidum salazinicum continens. TYPus: VENEZUELA. EDO. MERIDA. La Carbonera, etween La Azulita and Merida City, 2,200 m, 1 March 1975, Lopez-Figueiras & Keogh 9185 (MERF, holotypus). Thallus corticolous, pendulous, to 12.5 cm long, tufted from holdfast, densely branched, branching mainly dichotomous, with small cilia in apices; laciniae ochre-colored, subshiny, flat to canaliculate, more or less filamentous, 0.6-0.8 mm broad; pseudocyphellae lateral or laminal, ellipsoid, raised on small tubercles, few sorediate; cortical tissue paraplectenchymatous, 6-8 jim thick, of thick-walled hyphae, becoming disrupted by pseudocyphellae, peripheral chondroid tissue sometimes forming continuous upper and lower layers, 34-40 /zm thick, medulla dense. Pycnidia not seen. Photobiont protococcoid, cells subglobose to globose, 5.5-9.1 gcm diam. Apothecia not seen. Chemistry (TLC). Salazinic acid. Distribution and habitat. -Ramalina andina is known from subalpine altitudes (2,200-2,500 m) on the mountains of eastern and central Merida state but may have been overlooked elsewhere. It is found in very moist rain forests, growing on trees and shrubs. Ramalina andina is anatomically and chemically similar to R. pacifica Asah. (Stevens 1987). Both species are characterized by cortical tissue disrupted by pseudocyphellae, and salazinic acid as a chemical 0007-2745/94/26-33$0.95/0 This content downloaded from 157.55.39.221 on Sun, 06 Nov 2016 04:59:28 UTC All use subject to http://about.jstor.org/terms 1994] MARCANO & MORALES: RAMALINA SPP. NOV. FROM VENEZUELA 27
Read full abstract