Mercury-contaminated Soil Research Articles

Penerapan Metode Enhanced Phytoremediation pada Lahan Bekas Tambang Emas Desa Tirta Kencana Kecamatan Bengkayang

Community gold mining activities using the amalgamation method cause soil pollution due to the use of mercury (Hg) to extract gold content which causes a decrease in soil quality and environmental damage. Enhanced phytoremediation is a method that can be applied to remove mercury levels with using the Cyperus kyllingia plant and adding supporting elements in the form of bacterial isolates that are resistant to mercury. This research aimed to determine the effectiveness of Cyperus kyllingia plants and bacterial isolates for removing mercury levels in mercury-contaminated soil, as well as observing the bacterial population and plant morphology during the research. Based on the research results, it is known that the best efficiency value for removing mercury levels is found in phytoremediation with the addition of bacterial isolates, namely 86.88%. The largest bacterial population was found in the phytoremediation reactor with the addition of bacterial isolates, namely 73.3 × 106 CFU/mL. The mercury content in polluted soil causes changes in plant morphology, namely the color of green plants changes to yellow, but plants can reproduce as indicated by an increase in the number of leaves and plant stems

Characteristics and risk assessments of mercury pollution in a county region undergoing industrialization in northeastern China: a case study in Gongzhuling

Mercury pollution in emerging industrial zones and surrounding areas, especially in industrial concentration areas, has attracted much attention. So as to clarify the characteristics of Hg in the environment in China’s small and medium-sized industrial emerging and surrounding areas, central urban area of Gongzhuling, a traditional agricultural town with a high degree of industrialization in northeast Changchun industrial base, is taken as the scope. Geological accumulation trin (Igeo) was used to study the degree of soil mercury contamination in Gongzhuling area, latent Er (ecological risk) trin was used to appraise the Er of soil Hg in the study area, and non-carcinogenic risk assessment of mercury in soil using human exposure risk assessment model. The results showed that 34% of soil samples had higher Hg content than the background value of the province’s soil (0.04 mg kg−1). The Igeo results showed that Hg pollution rate of soil in study area was 9% (index >0). In conclusion, the level of soil Hg pollution in Gongzhuling area was low, and the pollution area is mainly concentrated in the northwestern part of the study range. The highest and lowest Er values of soil Hg from the study sample were 2.23 and 214.83, and 24% of the samples had Er > 40, that means they pose a moderate or higher potential ecological risk, and most of these points are located in the northeast of the study range. The main route of human exposure to Hg is oral ingestion. The HQ (non-carcinogenic risk index) and HI (total non-carcinogenic risk value) of soil mercury were both much less than 1, it indicates that the present level of soil Hg in the study range does not pose a threat to local adult health for the time being. This study provides reference for other urban pollution risk assessment, and further defines the direction of future work.

Digestate Improves Stinging Nettle (Urtica dioica) Growth and Fiber Production at a Chlor-Alkali Site.

Marginal lands have been proposed to produce non-food crop biomass for energy or green materials. For this purpose, the selection, implementation, and growth optimization of plant species on such lands are key elements to investigate to achieve relevant plant yields. Stinging nettle (Urtica dioica) is a herbaceous perennial that grows spontaneously on contaminated lands and was described as suitable to produce fibers for material applications. Two mercury-contaminated soils from industrial wastelands with different properties (grassland soil and sediment landfill) were used in this study to assess the potential growth of stinging nettle in a greenhouse mesocosm experiment. Two organic amendments were studied for their impact on nettle growth. The solid digestate from organic food wastes significantly doubled plant biomass whereas the compost from green wastes had a lower impact. The highest doses of organic amendments significantly increased the number of fibers, which doubled following digestate application, while reducing leaf Hg concentration. Both amendments significantly improved soil respiration and enzymatic activities linked to the microbial biomass in the soil from the sediment landfill by the end of the experiment. In the context of a phytomanagement scenario, solid digestate would be a preferred amendment resource to improve nettle production on industrial wastelands.

Perubahan Sifat Kimia Tanah Tercemar Merkuri Dengan Berbagai Modifikasi Pemberian Biochar Dan Tanaman Akar Wangi (Vetiveria zizanioides (L.)

This research aims to determine changes in the chemical properties of mercury-contaminated soil with various modifications in the application of biochar and vetiver grass (Vetiveria zizanioides (L.). This research uses an experimental method with an ex-situ remediation approach which was carried out from October 2023 to January 2024 at greenhouse. Sampling of mercury-contaminated soil was carried out on former traditional gold mining land in Segubuk Hamlet, Kuang Village, Taliwang District, West Sumbawa Regency. Soil sampling was carried out at 13 locations determined by purposive sampling and carried out using a soil drill soil (pH H2O, C-organic and CEC) as well as analysis of the total concentration of mercury (Hg) in the soil before and after the experiment. The results showed that the application of biochar alone and biochar planted with vetiver grass (Vetiveria zizanioides (L.) had a significant effect) on changes in soil chemical properties and is effective in reducing the concentration of mercury (Hg) in the soil. Biochar application can improve soil chemical properties by increasing pH, organic C, and soil CEC. On the other hand, the release of H+ ions from the carboxyl functional group (-COOH) in biochar has the opportunity to form complexes with heavy metal ions so that it can reduce their concentration in the soil.

Sulfur/zinc co-doped biochar for stabilization remediation of mercury-contaminated soil: Performance, mechanism and ecological risk

In this study, a novel sulfur/zinc co-doped biochar (SZ-BC) stabilizer was successfully developed for the remediation of mercury-contaminated soil. Results from SEM, TEM, FTIR and XRD revealed that biochar (BC) was successfully modified by sulfur and zinc. In the batch adsorption experiments, the sulfur-zinc co-pyrolysis biochar displayed excellent Hg(II) adsorption performance, with the maximum adsorption capacity of SZ-BC (261.074 mg/g) being approximately 16.5 times that of BC (15.855 mg/g). Laboratory-scale static incubation, column leaching, and plant pot experiments were conducted using biochar-based materials. At an additional dosage of 5 % mass ratio, the SZ-BC exhibits the most effective stabilization of mercury in soil, leading to a significant reduction in leaching loss compared to the control group (CK) by 51.30 %. Following 4 weeks of incubation and 2 weeks of leaching with SZ-BC, the residual mercury in the soil increased by 27.84 %. As a result, potential ecological risk index of mercury decreased by 92 % compared to the CK group. In the pot experiment, SZ-BC significantly enhanced the growth of Chinese cabbage, with biomass and root dry weight reaching 3.20 and 2.80 times that of the CK group, respectively. Additionally, the Translocation Factor (TF) and Bioconcentration Factor (BF) were reduced by 44.86 % and 74.43 %, respectively, in the SZ-BC group compared to the CK group. Moreover, SZ-BC can effectively improve enzyme activities and increase microbial communities in mercury-contaminated soils. The mechanisms of adsorption and stabilization were elucidated through electrostatic adsorption, ion exchange, surface complexation, and precipitation. These findings provide a potentially effective material for stabilizing soils contaminated with mercury.

Green remediation of mercury-contaminated soil using iron sulfide nanoparticles: Immobilization performance and mechanisms, effects on soil properties, and life cycle assessment

Mercury (Hg) pollution in soil has grown into a severe environmental issue. Effective in situ immobilization techniques are crucially demanded. In this study, we explored the application of carboxymethyl cellulose stabilized iron sulfide nanoparticles (CMC-FeS) for in situ immobilization of Hg in soil. CMC-FeS (a CMC-to-FeS molar ratio of 0.0004) was prepared via the reaction between FeSO4 and Na2S using CMC as a stabilizer. Remedying the Hg-polluted soil using 0.03 % CMC-FeS via batch experiments effectively reduced the acid leachable Hg by 97.5 % upon equilibrium after 71 days. Column elution tests demonstrated that the addition of CMC-FeS decreased the peak Hg concentration by 89.9 % and the total Hg mass eluted by 94.9 % after 523 pore volumes. CMC-FeS immobilized Hg in soil via chemical precipitation, ion exchange, and surface complexation. After the CMC-FeS treatment, Hg was transformed from more available exchangeable, carbonate-bound, and organic material-bound forms into the less available residual fraction, reducing the environmental risk of soil Hg from medium to low. The application of CMC-FeS boosted the soil enzyme activities and enhanced the soil bacterial diversity whereas decreased the production of methylmercury. CMC-FeS also facilitated long-term immobilization of Hg in soil. The acid leachable Hg and relative Hg bioaccessibility was decreased. Lift cycle assessment indicated that the preparation and application of CMC-FeS for in situ Hg remediation in soil met green chemistry principles. The present study confirms that CMC-FeS can be applied as an efficient and “green” amending agent for long-term Hg immobilization in soil/sediment.

Investigating the role of poly-γ-glutamic acid in Pennisetum giganteum phytoextraction of mercury-contaminated soil

Farmland mercury (Hg) pollution poses a significant threat to human health, but there is a lack of highly efficient phytoextraction for its remediation at present. This study investigates the impact of poly-γ-glutamic acid (γ-PGA) on the phytoextraction capabilities of Pennisetum giganteum (P. giganteum) in Hg-contaminated soil. Our research indicates that amending γ-PGA to soil markedly enhances the assimilation of soil Hg by P. giganteum and transformation of Hg within itself, with observed increases in Hg concentrations in roots, stems, and leaves by 1.1, 4.3, and 18.9 times, respectively, compared to the control. This enhancement is attributed to that γ-PGA can facilitate the hydrophilic and bioavailable of soil Hg. Besides, γ-PGA can stimulate the abundance of Hg-resistance bacteria Proteobacteria in the rhizosphere of P. giganteum, thus increasing the mobility and uptake of soil Hg by P. giganteum roots. Moreover, the hydrophilic nature of Hg-γ-PGA complexes supports their transport via the apoplastic pathway, across the epidermis, and through the Casparian strip, eventually leading to immobilization in the mesophyll tissues. This study provides novel insights into the mechanisms of Hg phytoextraction, demonstrating that γ-PGA significantly enhances the effectiveness of P. giganteum in Hg uptake and translocation. The findings suggest a promising approach for the remediation of Hg-contaminated soil, offering a sustainable and efficient strategy for environmental management and health risk mitigation.

Costus speciosus (Koen ex. Retz.) Sm.: a suitable plant species for remediation of crude oil and mercury-contaminated soil.

The aim of this study was to evaluate the efficiency of Costus speciosus (Koen ex. Retz.) Sm. in the degradation of crude oil and reduction of mercury (Hg) from the contaminated soil in pot experiments in the net house for 180days. C. speciosus was transplanted in soil containing 19150mgkg-1 crude oil and 3.2mgkg-1 Hg. The study includes the evaluation of plant biomass, height, root length, total petroleum hydrocarbon (TPH) degradation, and Hg reduction in soil, TPH, and Hg accumulation in plants grown in fertilized and unfertilized pots, chlorophyll production, and rhizospheric most probable number (MPN) at 60-day interval. The average biomass production and heights of C. speciosus in contaminated treatments were significantly (p < 0.05) lower compared to the unvegetated control. Plants grown in contaminated soil showed relatively reduced root surface area compared to the uncontaminated treatments. TPH degradation in planted fertilized, unplanted, and planted unfertilized pot was 63%, 0.8%, and 38%, respectively. However, compared to unvegetated treatments, TPH degradation was significantly higher (p < 0.05) in vegetated treatments. A comparison of fertilized and unfertilized soils showed that TPH accumulation in plant roots and shoots was relatively higher in fertilized soils. Hg degradation in soil was significantly (p < 0.05) more in planted treatment compared to unplanted treatments. The fertilized soil showed relatively more Hg degradation in soil and its accumulation in roots and shoots of plants in comparison to unfertilized soil. MPN in treatments with plants was significantly greater (p < 0.05) than without plants. The plant's ability to produce biomass, chlorophyll, break down crude oil, reduce Hg levels in soil, and accumulate TPH and Hg in roots and shoots of the plant all point to the possibility of using this plant to remove TPH and Hg from soil.

Influence of rice straw biochar addition on soil traits and nutrient uptake of Artichoke (Cynara cardunculus)grown in Mercury-contaminatedsoils

The present pot experiment was therefore conducted to investigate the effect of biochar application on the selected properties of mercury polluted soils and uptake of Artichoke (Cynara cardunculus) grown in polluted soils.Biochar obtained from rice straw was added at various rates (0, 5, 10 t/ha) on the soil artificially contaminated with mercury at a different concentration (0, 10, 20 ppm). The research indicated a significant (P &lt; 0.01) improvement in electrical conductivity, pH, available phosphorus, total nitrogen, exchangeable bases, cation exchange capacity and organic carbon owing to biochar addition.Additionally, uptake of potassium, phosphorus and nitrogen by Artichoke (Cynara cardunculus) was significantly enhanced by biochar application. A significant decrease in uptake of mercury owing to biochar application was also noticed in heavily mercury-contaminated soil (20 ppm). Hence, biochar application is mostvital to improve soil quality and fertility, improve nutrients uptake, amend Hg contaminated soil and decrease the quantity of carbon formedowing to the biomassburning.

Remediation of Mercury-contaminated Soil by Thermal Desorption Technique in a Laboratory-scale Muffle Furnace

Remediation of Mercury-contaminated Soil by Thermal Desorption Technique in a Laboratory-scale Muffle Furnace

Human Health Risk Assessment from Mercury-Contaminated Soil and Water in Abu Hamad Mining Market, Sudan.

Artisanal and small-scale gold mining (ASGM) poses a significant global threat due to mercury emissions and resulting health hazards. This study focuses on assessing these risks in the Abu Hamad ASGM community in Sudan. Utilizing the Mercury Analyzer 3000 (NIC), analyses of twelve soil samples (including one tailings sample) and seven water samples revealed the highest concentrations near amalgam burning locations: 34.8 mg/kg in soil (S06) and 3.26 µg/L in water (W03). Concentrations decrease with distance, with soil near burning exceeding tailings (S05 = 19.0 mg/kg). Hazard quotients indicate mercury vapor inhalation as the primary exposure route from soil, with the Hazard Index reaching 5.34 for adults and 33.4 for children close to amalgam burning sites. Water samples generally pose little risk except for W03, where children face potential danger via ingestion (HI = 1.74). These findings emphasize the urgent need for adopting retorts and eco-friendly practices to reduce mercury emissions and protect ASGM communities.

Emilia fosbergii Nicolson, a novel and effective accumulator for phytoremediation of mercury-contaminated soils

Soil contamination by toxic metals threatens global public health, highlighting the need for cost-effective and ecologically sound site remediation. In this study, we assessed phytoremediation of Hg-contaminated soils by Emilia fosbergii Nicolson (Asteraceae). Pot experiment was conducted using a substrate of sand and vermiculite (1:1 volume ratio), treatments consisted of five Hg concentrations (0, 1, 3, 5, and 7 mg kg−1). Metal transfer rates were calculated, including accumulation (BAF), translocation (TF) and bioconcentration (BCF) factors. E. fosbergii roots exhibited greater Hg accumulation than other tissues, but biomass production and plant health were not significantly affected at the concentrations tested, as indicated by elongation factors and tolerance index. The results revealed BAF values between 2.18 and 7.14, TF values ranged between 0.15 and 0.52, and the BCF index varied between 8.97 and 26.58. Treatments with Hg content of 5 mg kg−1 and 7 mg kg−1 recorded the highest total Hg concentrations of 66 mg kg−1 and 65.53 mg kg−1 (roots), and 9.18 mg kg−1 and 33.88 mg kg−1 (aerial), respectively. E. fosbergii demonstrated promise for Hg phytoremediation due to its high accumulation capacity, indicated by regular TF and high BCF and BAF indexes, thus classifying it as a high Hg accumulator.

Microbe-Mediated Impact on Cell Membrane Stability and Leaf Pigmentation in Pearl Millet under Toxic Mercury Stress in Soil

Pearl millet, Pennisetum glacum treated with Mycorrhiza, Rhizobium, and Trichoderma microbes, could grow in highly contaminated mercury soil. Microbe-mediated tolerance of this plant and the mechanism involved was studied after growing pearl millet in 100 ppm of mercury-contaminated soil for 120 days. The different parameters analyzed were associated with photosynthesis and the stability of the cell membrane. Mercury contamination led to the inhibition of growth in the leaves and reduced photosynthesis and membrane stability. The defensive mechanism of P. glacum under mercury stress was aided by Mycorrhiza, Rhizobium, and Trichoderma and Hg-related toxicity in the soil was averted. Photosynthetic parameters such as chlorophyll a b, carotenoids, and anthocyanin were quantified. The stability and injury of the cell membrane were measured. The results showed a significant role of microbes in improving the tolerance of P. glacum against metal stress in the soil.. KEYWORDS :Antioxidants, Chlorophyll, Mycorrhiza, Pearl millet, Trichoderma, Rhizobium

Complex interactions between diverse mobile genetic elements drive the evolution of metal-resistant bacterial genomes.

In this study, we compared the genomes of three metal-resistant bacteria isolated from mercury-contaminated soil. We identified diverse and novel MGEs with evidence of multiple LGT events shaping their genomic structure and heavy metal resistance. Among the three metal-resistant strains, Sphingobium sp SA2 and Sphingopyxis sp SE2 were resistant to multiple metals including mercury, cadmium, copper, zinc and lead. Pseudoxanthomonas sp SE1 showed resistance to mercury only. Whole genome sequencing by Illumina and Oxford Nanopore technologies was undertaken to obtain comprehensive genomic data. The Sphingobium and Sphingopyxis strains contained multiple chromosomes and plasmids, whereas the Pseudoxanthomonas strain contained one circular chromosome. Consistent with their metal resistance profiles, the strains of Sphingobium and Sphingopyxis contained a higher quantity of diverse metal resistance genes across their chromosomes and plasmids compared to the single-metal resistant Pseudoxanthomonas SE1. In all three strains, metal resistance genes were principally associated with various novel MGEs including genomic islands (GIs), integrative conjugative elements (ICEs), transposons, insertion sequences (IS), recombinase in trio (RIT) elements and group II introns, indicating their importance in facilitating metal resistance adaptation in a contaminated environment. In the Pseudoxanthomonas strain, metal resistance regions were largely situated on a GI. The chromosomes of the strains of Sphingobium and Sphingopyxis contained multiple metal resistance regions, which were likely acquired by several GIs, ICEs, numerous IS elements, several Tn3 family transposons and RIT elements. Two of the plasmids of Sphingobium were impacted by Tn3 family transposons and ISs likely integrating metal resistance genes. The two plasmids of Sphingopyxis harboured transposons, IS elements, an RIT element and a group II intron. This study provides a comprehensive annotation of complex genomic regions of metal resistance associated with novel MGEs. It highlights the critical importance of LGT in the evolution of metal resistance of bacteria in contaminated environments.

Modified biochar enhances soil fertility and nutrient uptake and yield of rice in mercury-contaminated soil

Biochar, as an emerging soil remediation technology, has gained substantial attention. However, the impact of modified biochar on soil fertility, nutrient uptake, and rice yield in mercury (Hg)-contaminated soil remains unclear. This study explores the influence of biochar on soil fertility, nutrient absorption, photosynthesis, and rice yield in Hg-contaminated soil. We analyzed soil physicochemical properties, nutrient distribution (nitrogen, phosphorus and potassium) in roots, stems, and leaves, as well as plant gas exchange traits and yield components to provide a comprehensive assessment. Two experiments were conducted using original biochar (BC), produced via direct combustion and rice husk cracking, along with six types of modified biochar [amino (BCNH2), epoxy (BCCH(O)CH), ethoxy (BCC2H5O), sulfhydryl (BCSH), selenium (BCSe) loaded, and chitosan (BCchitosan)]. These biochars were applied at four different concentrations (0.07 %, 0.14 %, 0.20 % and 0.35 %). Comparative analysis against the control revealed that the application of modified biochar, particularly BCNH2, BCC2H5O and BCSH, significantly enhanced soil organic matter and plant nutrients (total N, available P, available K). Furthermore, it promoted the uptake of N, P, and K by rice leaves. The biochar addition exhibited a positive impact on rice photosynthesis and yield within a specific range of concentrations. A comprehensive analysis indicates that soil fertility, photosynthesis, and rice yield were significantly enhanced when BCNH2, BCC2H5O, and BCSH were applied at 0.20 % under the study conditions. These findings advance our understanding of how biochar affects soil fertility, nutrient uptake, and rice yield, offering valuable insights for practical agricultural and environmental management in Hg-contaminated fields.

Effective reducing the mobility and health risk of mercury in soil under thiol-modified biochar amendment

Soil mercury contamination poses health risks. However, effective immobilization techniques are lacking with challenges including low efficiency, limited long-term stability, susceptibility to multi-medium interference, and difficulty in controlling health risks. This study confirmed the feasibility of thiol-modified biochar, and elucidated the underlying mechanisms. Within 32 days of treatment, the leachable mercury decreased from 184.7 μg/L to below the hazardous waste threshold (100 μg/L, HJ/T299–2007, China). After 198 days of treatment, the soil achieved a safe ecological state with a mercury immobilization rate of 79.8–98.2% and a 50% reduction in available methylmercury. Thiol-modified biochar facilitated the conversion of active mercury species (exchangeable, carbonate, and oxide) into stable forms (organic and residual) through complexation and precipitation (e.g., HgS). Soil quality improvements were observed, including enhanced cation exchange capacity, available nitrogen, and total organic carbon. Thiol-modified biochar exhibits long-term effectiveness. After one and two years of treatment, the leachable mercury remained within acceptable health risk limit (hazard quotient < 1) for adults and children, respectively. After three years, the leachable mercury met the Level III groundwater quality standard (< 1 μg/L, GB 14848–2017, China). This study demonstrates an effective strategy for long-term diminishing mercury mobility and health risks in soil.

Influence of nanoscale sulfur on mercury accumulation and plant growth in oilseed rape seedlings (Brassica napus L.) grown on mercury-contaminated soil

Mercury (Hg) pollution has seriously threatened the crop productivity and food security. In the present research, experiments were conducted to assess the influence of nanoscale sulfur/sulfur nanoparticles and the corresponding bulk and ionic sulfur forms on the growth and Hg accumulation of oilseed rape seedlings grown on Hg-contaminated soil, as well as the transformation of soil Hg fractions. The results showed a significant reduction in fresh biomass for seedlings grown on 80–200 mg/kg Hg-polluted soil after 30 days. At 120 mg/kg Hg treatment, 100–300 mg/kg sulfur nanoparticles (SNPs) application counteracted Hg toxicity more effectively compared to the corresponding bulk sulfur particles (BSPs) and ionic sulfur (sulfate) treatments. The seedlings treated with 120 mg/kg Hg + 300 mg/kg SNPs gained 54.2 and 56.9% more shoot and root biomass, respectively, compared to those treated with Hg alone. Meanwhile, 300 mg/kg SNPs application decreased Hg accumulation by 18.9 and 76.5% in shoots and roots, respectively, relative to Hg alone treatment. SNPs treatment caused more Hg to be blocked in the soil and accumulating significantly less Hg in plants as compared to other S forms. The chemical fractions of Hg in the soil were subsequently investigated, and the solubility of Hg was significantly decreased by applying SNPs to the soil. Especially 200–300 mg/kg SNPs treatments caused the ratio of the soluble/exchangeable and the specifically absorbed fraction to be the lowest, accounting for 1.95–4.13% of the total Hg of soil. These findings suggest that adding SNPs to Hg-contaminated soils could be an effective measure for immobilizing soluble Hg and decreasing the Hg concentration in the edible parts of crops. The results of the current study hold promise for the practical application of SNPs to Hg-contaminated farmland for better yields and simultaneously increasing the food safety.

Elevated methylmercury production in mercury-contaminated soil and its bioaccumulation in rice: key roles of algal decomposition

Elevated methylmercury production in mercury-contaminated soil and its bioaccumulation in rice: key roles of algal decomposition

The growth of mercury-resistant bacterial consortium at different levels of pH, temperature, and C:N ratio

The study was conducted to investigate the growth of mercury-resistant bacterial consortium under the influence of different pHs, temperatures, and C:N ratios, as a part of mercury bioremediation research. The consortium consisted of five bacterial isolates selected due to their resistance to HgCl2 up to 100 ppm and was proven to effectively reduce mercury from the culture media. Consortium suspension was inoculated into two sets of media: 1) Tryptic soy broth (TSB) with pHs of 4.8, 6.1, 8.5, and 9.7; 2) Mineral salt with the addition of glucose and peptone to create C:N ratios of 20:1, 30:1, and 40:1 while the pH was set to 7.4. For comparison, a solution containing mercury-contaminated soil was added to the same set of media and regarded as a microbial community. The growth curves show that the bacterial consortium tended to grow better compared to the microbial community, especially in the TSB medium at incubation temperatures of 20 and 35 °C. In the same medium at a temperature of 45 °C, the growth of both consortium and community under high pHs (8.5 and 9.7) was higher than the one under lower pHs (4.8 and 6.1). Meanwhile, in the mineral salt medium, the influence of the inoculant was not remarkable except under an incubation temperature of 35 °C. Moreover, the C:N ratio did not affect the growth of either the bacterial consortium or the microbial community (p > 0.05). The variance analysis also indicated that the growths were significantly different only when the pH was 4.8.

Reduced Antibiotic Resistance in the Rhizosphere of Lupinus albus in Mercury-Contaminated Soil Mediated by the Addition of PGPB.

The emergence of antibiotic resistance (AR) poses a threat to the "One Health" approach. Likewise, mercury (Hg) pollution is a serious environmental and public health problem. Its ability to biomagnify through trophic levels induces numerous pathologies in humans. As well, it is known that Hg-resistance genes and AR genes are co-selected. The use of plant-growth-promoting bacteria (PGPB) can improve plant adaptation, decontamination of toxic compounds and control of AR dispersal. The cenoantibiogram, a technique that allows estimating the minimum inhibitory concentration (MIC) of a microbial community, has been postulated as a tool to effectively evaluate the evolution of a soil. The present study uses the metagenomics of 16S rRNA gene amplicons to understand the distribution of the microbial soil community prior to bacterial inoculation, and the cenoantibiogram technique to evaluate the ability of four PGPB and their consortia to minimize antibiotic resistance in the rhizosphere of Lupinus albus var. Orden Dorado grown in Hg-contaminated soils. Results showed that the addition of A1 strain (Brevibacterium frigoritolerans) and its consortia with A2, B1 and B2 strains reduced the edaphic community´s MIC against cephalosporins, ertapenem and tigecycline. The metagenomic study revealed that the high MIC of non-inoculated soils could be explained by the bacteria which belong to the detected taxa,. showing a high prevalence of Proteobacteria, Cyanobacteria and Actinobacteria.