Mentha Rotundifolia Research Articles

Chemical composition and in vitro antimicrobial, insecticidal and antioxidant activities of the essential oils of Mentha pulegium L. and Mentha rotundifolia (L.) Huds growing in Algeria

The essential oils of Mentha pulegium L. (MPE) and Mentha rotundifolia (L.) Huds (MRE) which are growing in Algeria were prepared by hydrodistillation and their chemical compositions investigated by GC/MS. The oils were tested for their antimicrobial activity using disc-diffusion and spot assays, antioxidant activity using 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) test and Kit Radicaux Libres® (KRL) biological assay. Also, contact toxicity, fumigant toxicity and repellency tests of these essential oils were evaluated against adults of Rhyzopertha dominica (F.) (the principal pest of wheat). The major components found in MPE are pulegone (70.4%), neo-menthol (13.4%), neo-menthol acetate (3.5%) and menthone (2.7%). On the other hand, MRE provided trans-piperitone epoxide (30.2%), piperitone oxide (8.7%), thymol (4.5%), germacrene D (3.5%) and terpinen-4-ol (2.7%) as major ingredients. MRE exhibited stronger antimicrobial effect and antioxidant activity in the KRL test than MPE. In the contact assay, DL50 values of MRE and MPE were 3.3 and 6.9μL/mL, respectively. Fumigant toxicity assay of MPE and MRE showed mortality ratio of 39.2 and 44.3%, respectively at the dose of 2μL/mL. Moreover, at this dose and after 30min exposure time, the repellent effect showed death rates of 46.03% and 47.54% for MPE and MRE, respectively. As conclusion, MPE and MRE are potential alternatives to chemical additives in food and pharmaceutical industries.

Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

The leaves of three Mentha species harvested in Algeria, Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR) were examined for their content in polyphenols and for some activities-linked biological properties these could impart. The contents in total phenolics (TPC) and flavonoids (TFC) were evaluated by the Folin–Ciocalteu and the aluminum chloride methods, respectively. Whereas MS showed the highest TPC (12.0±0.3mg gallic acid equivalents/g of dry weight), MR had the highest content in TFC (3.3±0.1mg quercetin equivalents of dry weight). The pharmacological properties of these extracts were evaluated by assessing in vitro their antioxidant and antityrosinase activities. The modulation of mushroom tyrosinase activity was measured by colorimetry of the melanins formed in the presence of tyrosine. MS exhibited the strongest radical scavenging activity (RSA) in all assays: (i) the IC50s values to neutralize the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radicals (ABTS+) and the 2,2′-diphenyl-1-picrylhydrazyl radicals (DPPH) were 10.3±0.9 and 16.2±0.2μg/mL, respectively; and (ii) its original electrochemically measured superoxide quenching index value is 188±37μg/mL (AI50). MR however showed the highest tyrosinase inhibitory activity (IC50=108±20μg/mL). A silica gel thin-layer chromatography (TLC) technique revealed the presence of caffeic and rosmarinic acids and diosmin in all extracts. These results were confirmed by high performance liquid chromatography with diode array detection (HPLC/DAD).

The Oil from Mentha rotundifolia as Green Inhibitor of Carbon Steel Corrosion in Hydrochloric Acid

The corrosion inhibition potentials of Mentha rotundifolia oil on carbon steel in 1 M HCl was studied at different concentrations via gravimetric, electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization techniques. Polarization curves reveal that Mentha rotundifolia oil is a mixed-type inhibitor. Changes in impedance parameters (charge transfer resistance Rt, and double-layer capacitance Cdl) were indicative of adsorption of Mentha rotundifolia on the metal surface, leading to the formation of a protective film. The effect of the temperature on the corrosion behavior with addition of the optimal concentration of Mentha rotundifolia oil was studied in the temperature range 308 and 338 K. Adsorption of oil on the carbon steel surface is found to obey the Langmuir adsorption isotherm. Attempts to explain the inhibitory action were carried out using density functional theory (DFT) at B3LYP/6-31G(d,p) level. Quantum chemical parameters most relevant to its potential action as corrosion inhibitor such as EHOMO (highest occupied molecular orbital energy), ELUMO (lowest unoccupied molecular orbital energy), energy gap (ΔE), and Mulliken charges have been calculated and discussed. The theoretical results were found to be consistent with the experimental data.

Algerian mint species: high performance thin layer chromatography

Objective: To determine the quantitative paremeters of rosmarinic acid in Algerian mints, Mentha spicata L. (M. spicata), Mentha pulegium L. and Mentha rotundifolia (L.) Huds by high performance thin layer chromatography (HPTLC)-densitometric method and screen the effects of these plant extracts on linoleic acid peroxidation. Methods: The analyses were performed on HPTLC silica gel 60 F254 plates with chloroform: acetone: formic acid (75:16.5:8.5, v/v) as the mobile phase. Rosmarinic acid was determined in UV at 365 nm and fluorescence at λ exc 325 nm with a 550 nm filter, respectively. The effects of plants extracts on linoleic acid peroxidation were measured by an indirect in vitro colorimetric method. Results: Chromatographic resolution permitted reliable quantification in both measurement modes and calibration curves were linear in a range of 150-1 000 ng/spot. M. spicata was found to contain significantly higher concentrations of rosmarinic acid. The densitometric quantification allowed the analysis of many samples in a short time with reasonable precision (total precision for Mentha spp extracts, 5.1% and 5.8% for UV and fluorescence detection, respectively). The HPTLC data, allied to assays of linoleic acid peroxidation prevention, suggested the potential of M. spicata (52% Trolox襆 equivalents) as a natural source for inhibitors of lipid peroxidation. Conclusions: Densitometry can be used for routine determination and quality control of rosmarinic acid in herbal and formulations containing Mentha species. Peer reviewer

Effect of Drying on Essential Oil Yields and Chemical Composition of Pineapple Mint (Mentha rotundifolia ‘variegata’) from China

The fresh leaves of pineapple mint (Mentha rotundifolia ‘variegata’) were dried by air-drying at ambient temperature (22°C) and oven-drying at 60°C. The essential oils of each treatment were isolated by hydrodistillation (HD) and analysed by GC-MS and retention index comparison. The results showed that the yields of the oils were significantly affected by the drying methods. Higher oil yields by air-drying at 22°C was obtained when compared with oven-drying (0.63 % and 0.31 %, respectively), while the effect of drying methods on the chemical components of the essential oils were not significantly different. Thirty-three and thirty-one components, (99.06 % and 98.75 % of the total oils, respectively), were identified from the two oils. The main components were cis-jasmone (36.77 % by air-drying, 32.43 % by oven-drying), germacrene D (11.89 %, 10.88 %), β-ocimene (10.51 %, 11.27 %), viridiflorol (8.20 %, 8.47 %), octenyl acetate (6.41 %, 6.74 %), and β-farnesene (4.22 %, 5.66 %).

A Study on the Genetic base of Apple Mint (Mentha Rotundifolia L. (Huds.) in South India

Variability and diversity of Mentha rotundifolia L. (Huds.), an economically important aromatic culinary herb was analyzed based on observations on nine salient growth characters recorded from 37 accessions of the species collected from various locations of its traditional growing areas in Tamil Nadu and Karnataka. Among the nine characters studied, only two characters such as shoot weight and internodal length showed statistically significant variation between the accessions. Other attributes like plant height, number of primary branches, number of nodes, number of leaves, leaf length, leaf breadth and leaf area did not vary significantly between the accessions. Such non significant variability with respect to majority of the morphological characters indicates the narrow genetic base of the species in its traditional area of cultivation and the possible threat of the crop becoming incapable of overcoming biotic and abiotic stresses and change in climatic conditions.

Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia L. in Tunisia

The present study is the first investigation of the chemical composition, antioxidant and antimicrobial activities of Mentha rotundifolia L. essential oils in Tunisia. Results show that essential oils from Beja locality were most complex and present 45 compounds representing 96.83% of the total oil composition. The major components of the studied oils in this site are β-Caryophyllene (26.67%), Germacrene D (12.31%) and Carveol (7.38%). 40 components representing 95.81% of the total oil were identified in Bizerte site. Those essential oils are dominated by Pulegone (32.09%), Piperitenone oxide (17.28%) and 5-Acetyl Thiazole (11.26%). Considerable levels of antioxidant activities of the investigated essential oils were highlighted. Variations in antioxidant activities may be attributed to the concentrations of major components and the presence of some phenolic compounds like Diosphenol and 2-Allyl-4-methylphenol. Our results showed strong activities of the investigated oils against all tested microorganisms. The highest antimicrobial activities were observed against Gram+ bacteria followed by Gram− ones then fungal species.

Fungicide Activity ofThymus mastichinaandMentha rotundifoliain PlantsIn Vitro

Journal of Medicinal FoodVol. 16, No. 4 Letter to the EditorFungicide Activity of Thymus mastichina and Mentha rotundifolia in Plants In VitroFernanda Leal, Ana Cláudia Coelho, Tatiana Soriano, Carina Alves, and Manuela MatosFernanda LealInstitute for Biotechnology and Bioengineering, Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.Search for more papers by this author, Ana Cláudia CoelhoVeterinary Microbiology Laboratory, Department of Veterinary Sciences, Animal and Veterinary Research Center, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.Search for more papers by this author, Tatiana SorianoDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.Search for more papers by this author, Carina AlvesDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.Search for more papers by this author, and Manuela MatosInstitute for Biotechnology and Bioengineering, Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.Search for more papers by this authorPublished Online:8 Apr 2013https://doi.org/10.1089/jmf.2012.0164AboutSectionsView articleView Full TextPDF/EPUB ToolsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail View article"Fungicide Activity of Thymus mastichina and Mentha rotundifolia in Plants In Vitro." , 16(4), p. 273FiguresReferencesRelatedDetailsCited byThymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity19 December 2020 | Pharmaceuticals, Vol. 13, No. 12Chemical composition and biological activity of Mentha citrata Ehrh., essential oils growing in southern Algeria8 August 2019 | Journal of Food Science and Technology, Vol. 56, No. 12Thymus spp. plants - Food applications and phytopharmacy propertiesTrends in Food Science & Technology, Vol. 85Contribution of the main polyphenols of Thymus mastichina subsp. mastichina to its antioxidant propertiesIndustrial Crops and Products, Vol. 66 Volume 16Issue 4Apr 2013 InformationCopyright 2013, Mary Ann Liebert, Inc. and Korean Society of Food Science and NutritionTo cite this article:Fernanda Leal, Ana Cláudia Coelho, Tatiana Soriano, Carina Alves, and Manuela Matos.Fungicide Activity of Thymus mastichina and Mentha rotundifolia in Plants In Vitro.Journal of Medicinal Food.Apr 2013.273-273.http://doi.org/10.1089/jmf.2012.0164Published in Volume: 16 Issue 4: April 8, 2013PDF download

Inhibitive effect by extract of Mentha rotundifolia leaves on the corrosion of steel in 1 M HCl solution

An extract of Mentha rotundifolia leaves (EMRL) was tested as a corrosion inhibitor of steel in 1 M HCl using electrochemical impedance spectroscopy, Tafel polarization methods, and weight loss measurements. The inhibition efficiency of the extract of Mentha rotundifolia leaves was calculated and compared. We note good agreement between these methods. The results obtained revealed that the inhibitor tested differently reduced the kinetics of the corrosion process of steel. Its efficiency increases with the concentration and attained 92.87 % at 35 %. The effect of temperature on the corrosion behavior of steel in 1 M HCl was also studied in the range 298 and 338 K. The thermodynamic data of activation were determined. Mentha rotundifolia extract is adsorbed on the steel surface according to a Langmuir adsorption model.

Antiproliferative Activity of Flower Hexane Extract Obtained from Mentha spicata Associated with Mentha rotundifolia Against the MCF7, KB, and NIH/3T3 Cell Lines

This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3.

Antibacterial Activity of Essential Oils of Some Algerian Aromatic Plants Against Multidrug Resistant Bacteria

Antibacterial activity of essential oils from four Lamiaceae aromatic plants (Thymus capitatus L. Hoffmann and Link, Lavandula dentata L., Salvia officinalis L. and Mentha rotundifolia L.), used in traditional folk medicine of Algeria were screened against three resistant bacterial strains (Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa), which were isolated from hospitalized patients and standard strains of the same bacteria. The highest antibacterial potentiality was exhibited by the essential oil of leaf essential oil of T. capitatus, followed by that of S. officinalis. The leaf essential oil of T. capitatus can be considered to be as equally potent as the most effective antibiotics, such as ofloxacin, gentamicin, amikacin, and nalidixic acid. A sensitivity test performed with commonly used sensitivity test disks showed the appearance of multiple drug resistant phenotypes of the bacteria tested.

Moisture sorption isotherms and thermodynamic properties of tow mints: Mentha pulegium and Mentha rotundifolia

Moisture sorption isotherms of Mentha pulegium and Mentha rotundifolia were determined at 30, 40 and 50 °C using the static gravimetric method. GAB, modified Halsey and Peleg models were fitted to the experimental data and the agreement between experimental and predicted values was satisfactory. Calculation of the thermodynamic properties was further used to provide an understanding of the properties of water and energy requirements associated with the sorption behaviour. Isosteric heats of sorption were calculated through direct use of moisture isotherms by applying the Clausius-Clapeyron equation. The differential enthalpy and entropy decreased with increasing moisture content and were adequately characterized by a power law equation. A plot of differential heat versus entropy satisfied the enthalpy-entropy compensation theory.

Chemical Composition of the Leaf Oil of Mentha rotundifolia (L.) from Algeria

Two Algerian origin oils of Mentha rotundifolia (L.) Hud. were analyzed by means of GC and GC/MS. Piperitenone oxide (23.5–38.6%) and cis-piperitone oxide (28.1–30.5%) were the two main constituents of the oils analyzed.

Composition chimique et activités antimicrobienne et insecticide de l'huile essentielle de Mentha rotundifolia du Maroc

The chemical composition and the antimicrobial and insecticidal activities of the essential oil in Mentha rotundifolia were studied. The essential oil is rich in pulegone (85%). The susceptibility of bacteria (Escherichia coli, Bacillus subtilis, Staphylococcus aureus), fungi (Trametes pini, Aspergillus niger, Pénicillium parasiticus) and insects of cereal (Rhyzopertha dominica and Sitophilus oryzae) with respect to the essential oil of Mentha rotundifolia was studied at different concentrations. At the 1/1000V/V concentration, the essential oil inhibited all bacteria and fungi, whereas at 6,5.10−2 ml/cm3 concentration, the essential oil destroyed all cereals insects.

Essential oils of Mentha pulegium and Mentha rotundifolia from Uruguay

Essential oils obtained by hydrodistillation from leaves of Mentha pulegium L. and Mentha rotundifolia (L.) Huds. from Uruguay were analysed by GC-FID and GC-MS. Oxygen-containing monoterpenes were the main group of constituents in both oils. Pulegone, isomenthone and menthone were the major components in the oil of M. pulegium, whereas piperitenone oxide and (Z)-sabinene hydrate were the major ones in M. rotundifolia. Enantiomerically pure (-)-menthone, (+)-isomenthone, (+)-isomenthol, (-)-menthol and (+)-pulegone were detected by multidimensional gas chromatography in the case of M. pulegium oil.

BIOINDICATION OF HEAVY METALS WITH AQUATIC MACROPHYTES: THE CASE OF A STREAM POLLUTED WITH POWER PLANT SEWAGES IN POLAND

The Kozi Brod (left tributary of the Biala Przemsza, east of Katowice) flows in a highly industrial coal-mining area dominated by the power plant of Siersza. Concentrations of the microelements nickel (Ni), chromium (Cr), cobalt (Co), zinc (Zn), manganese (Mn), lead (Pb), cadmium (Cd), copper (Cu), barium (Ba), aluminum (Al), vanadium (V), and strontium (Sr), as well as the macronutrients nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), and sulfur (S), were measured in water and plants of the Kozi Brod. The collected plants were: Myosotis palustris L. Nathorst, Galium palustre L., Mentha rotundifolia L. Huds., Mentha aquatica L., Berula erecta (Huds.) Coville, Cardamine amara L., Epilobium angustifolium L., Geranium palustre L., Lysimachia vulgaris L., Crepis paludosa L. Much., Calitriche verna L., Solanum dulcamara L., and the aquatic moss Hygrohypnum ochraceum (Turn.) Loesk. These plants were used to evaluate the spatial distribution of elements in the Kozi Brod and contained elevated levels of Co, Cd, Zn, Ni, Mn, Al, Pb, and Cu. Significant correlations between concentrations of Cd, Zn, and Mn in water and plants indicate the potential of these species for pollution monitoring.

Nematicidal Activity of Essential Oils and Their Components Against the Root-Knot Nematode

ABSTRACT Nematicidal activity of essential oils extracted from 27 spices and aromatic plants were evaluated in vitro and in pot experiments. Twelve of the twenty-seven essential oils immobilized more than 80% of juveniles of the root-knot nematode Meloidogyne javanica at a concentration of 1,000 mul/liter. At this concentration, most of these oils also inhibited nematode hatching. Essential oils of Carum carvi, Foeniculum vulgare, Mentha rotundifolia, and Mentha spicata showed the highest nematicidal activity among the in vitro tested oils. These oils and those from Origanum vulgare, O. syriacum, and Coridothymus capitatus mixed in sandy soil at concentrations of 100 and 200 mg/kg reduced the root galling of cucumber seedlings in pot experiments. The main components of these essential oils were tested for their nematicidal activity. Carvacrol, t-anethole, thymol, and (+)-carvone immobilized the juveniles and inhibited hatching at >125 mul/liter in vitro. Most of these components mixed in sandy soil at concentrations of 75 and 150 mg/kg reduced root galling of cucumber seedlings. In 3-liter pot experiments, nematicidal activity of the essential oils and their components was confirmed at 200 and 150 mg/kg, respectively. The results suggest that the essential oils and their main components may serve as nematicides.

Chemical Composition of the Leaf Oil of Mentha rotundifolia (L.) Hudson from Cuba

The composition of the leaf oil of Mentha rotundifolia (L.) Hudson grown in Cuba was analyzed by GC/MS. Fifty compounds were identified, the main constituents being 2,4(8),6-p-menthatrien-2,3-diol (14.5%) and germacrene D (12.4%).

Two New Stereoisomers of 1,2-Epoxymenthyl Acetate from self-Pollinated Plant Oils of Mentha rotundifolia

The stereochemistries of two new compounds isolated from the self-pollinated oils of Mentha rotundifolia were elucidated to be (1S:2S:3R:4R)-(-)-1,2-epoxyisomenthyl acetate and (1S:2S:3S:4R)-(-)-1,2-epoxyneoisomenthyl acetate.

CNS activity of Mentha rotundifolia and Mentha longifolia essential oil in mice and rats

AbstractThe essential oils of Mentha rotundifolia and Mentha longifolia were analyzed with GC and GC/MS. The major components were rotundifolone (10.4%) and piperitol (57.6%) in M. rotundifolia, and rotundifolone (33.2%) and diosphenol (47.7%) in M. longifolia. M. longifolia essential oil exhibited a stronger CNS depressant effect than that of M. rotundifolia; both essential oils were somnifacients.