Mentha Rotundifolia Research Articles

Unveiling physiological responses and modulated accumulation patterns of specialized metabolites in Mentha rotundifolia acclimated to sub-tropical environment.

The online version contains supplementary material available at 10.1007/s12298-024-01489-8.

Hepatoprotective activity of Mentha rotundifolia aqueous extract against hepatocellular damage induced by CCL4 in rats

This study aims to assess hepatoprotective properties of M. rotundifolia. Decoction was used to prepare the aqueous extract. The preliminary cytotoxicity evaluated against Caco 2 and RAW 264 cells demonstrate no cytotoxic effect. The preventive impact of the extract against liver damage was evaluated by examining blood levels of AST, ALT, ALP, total proteins, and histological alterations in liver tissues. Thirty albino rats were separated into five groups: the first served as normal group, the second was injected by olive oil (3 ml/kg), and the third was injected by CCL4 (3 ml/kg). However, groups IV and V received daily doses of 250 and 500 mg extract/kg bw, respectively before CCL4 injection. The results showed that the administration of the extract led to a marked improvement in plasma biochemical markers and a reduction in symptoms of CCL4-induced liver damage. The extract exhibits hepatoprotective activity, which may be attributed to its phytochemical components.

Comparative study of chemical composition Antioxidant and Antimicrobial activity of Methanolic Extracts of Mentha rotundifolia

Comparative study of chemical composition Antioxidant and Antimicrobial activity of Methanolic Extracts of Mentha rotundifolia

Mentha rotundifolia (L.) Huds. and Salvia officinalis L. hydrosols mitigate aging related comorbidities in rats.

Aging is often linked to oxidative stress, where the body experiences increased damage from free radicals. Plants are rich sources of antioxidants, playing a role in slowing down aging and supporting the proper functioning and longevity of cells. Our study focuses on exploring the impact of Mentha rotundifolia (MR) and Salvia officinalis (SO) hydrosols on aging-related comorbidities. The chemical composition of MR and SO hydrosols was analyzed by gas chromatography coupled to mass spectrometry. 2,2-Diphenyl 1-picrylhydrazyl and 2,20-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid radicals scavenging assays were used to assess their in vitro antioxidant activity, and heat induced albumin denaturation test was used to evaluate their anti-inflammatory activity. Subsequently, we administered 5% of each plant hydrosol in the drinking water of 18-month-old rats for six months. We then conducted behavioral tests, including open field, dark/light box, rotarod, and Y-maze assessments, and measured biochemical parameters in plasma, liver and brain tissues. At two years old, animals treated with MR and SO hydrosols displayed fewer physical and behavioral impairments, along with well-preserved redox homeostasis in comparison with animals in the control group. These results highlighted the significance of MR and SO hydrosols in addressing various aspects of age-related comorbidities. The study suggests that these plant-derived hydrosols may have potential applications in promoting healthy aging and mitigating associated health challenges.

Chemical constituents and in vitro biological activities of Mentha rotundifolia’s essential oils extracted by ultrasound-assisted hydrodistillation compared to conventional hydrodistillation

Chemical constituents and in vitro biological activities of Mentha rotundifolia’s essential oils extracted by ultrasound-assisted hydrodistillation compared to conventional hydrodistillation

Inhibitory Effect of Essential Oil from Rond Left Mint (Mentha Rotundifolia) on the Corrosion of C35 Mild Steel in an Acidic Environment

Inhibitory Effect of Essential Oil from Rond Left Mint (Mentha Rotundifolia) on the Corrosion of C35 Mild Steel in an Acidic Environment

Different indole-3-acetic acid and 6 benzyl amino purine concentrations affect biomass, phenolic profile, and bioactivity in Mentha rotundifolia L.

Different indole-3-acetic acid and 6 benzyl amino purine concentrations affect biomass, phenolic profile, and bioactivity in Mentha rotundifolia L.

Is the activity of antioxidant enzymes, some biochemical properties and essential oil content affected by salinity stress in different species of mint?

Mint is one of the important medicinal plants with important properties in industry, medicine, and pharmacy. Salinity stress is one of the limiting factors for plant growth that also affects plants' physiological and biochemical properties. In this study, effects of salinity on biochemical and enzymatic properties of 18 ecotypes of mint from six different species, were studied. The experimental results showed that salinity and increased with increasing stress integrity influenced enzymatic properties, proline content, electrolyte leakage, hydrogen peroxide, malondialdehyde, and essential oil content. Cluster analysis and principal component analysis grouped the studied species on the basis of biochemical characteristics. Based on biplot results, piperita and rotundifolia showed better stress tolerance, but longifolia was identified as salt sensitive. The experimental results also showed that six species of the mint had diverse reactions in terms of biochemical properties at different levels of salinity stress.

Mentha rotundifolia (L.) Huds. aqueous extract attenuates H2O2 induced oxidative stress and neurotoxicity.

Oxidative stress plays a causal role in neurodegenerative diseases. The aim of this study is to evaluate the antioxidant and neuroprotective effects of Mentha rotundifolia (L.) Huds (M. rotundifolia), a widely used Moroccan plant in traditional medicine. The chemical composition of M. rotundifolia aqueous extract was analyzed by liquid chromatography coupled to mass spectrometry (LC-MS). 2,2-diphenyl 1-picrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) assays were used to assess its in vitro antioxidant activity. H2O2 was utilized to induce oxidative stress and neurotoxicity in vivo. Behavioral changes were evaluated using Open Field, Y-maze and Rotarod tests. Hyperalgesia was assessed using the tail immersion test. The LC-MS/MS analysis revealed high content of kaempferol glucuronide (85%) at the extract. IC50 values of the DPPH and ABTS were 26.47 and 41.21 μg/mL, respectively. Pre-treatments with M. rotundifolia extract attenuated the behavioral changes induced by H2O2. In addition, the latency of tail withdrawal increased significantly in the treated groups suggesting central analgesic effect of M. rotundifolia extract. Moreover, the extract attenuated the deleterious effects of H2O2 and improved all liver biomarkers. The obtained results suggested that M. rotundifolia had remarkable antioxidant and neuroprotective effects and may prevent oxidative stress related disorders.

Effects of Controlled Culture Conditions on Chemical Composition and Antimicrobial Activities of Mentha rotundifolia Essential Oils

The study objectives were the determination of Mentha rotundifolia essential oils composition using GC/MS analysis and the evaluation of their antimicrobial activities. In addition, the determination of the relationships between plants acclimatization conditions, essential oils composition and antimicrobial activities. Essential oils extracted via hydrodistillation method from wild plants and acclimatized plants in different culture conditions of Mentha rotundifolia. Five selected pathogenic microbial strains were used to evaluate EOs in vitro antimicrobial activities. Essential oils GC/MS analysis revealed the dominance of the oxygenated monoterpene (piperitenone oxide). A significant effect of plant culture conditions in acclimatization room on essential oils composition compared to the control was observed. We also noted that antimicrobial activities of extracted essential oils from acclimatized plants were higher than those from field-grown M. rotundifolia plants. Content of Piperitenone Oxide in acclimatized plantlets is 93.07% for plantlets cultured at 16°C and a photoperiod of 16 hours light / 8 hours dark, statistically higher than wild plants where it is around 78%. We also noted the presence of Limonene (5.7%) in plantlets grown at 35°C which is significantly different than that of the wild plants (1.55 %). Essential oils antimicrobial activities showed that the plantlets grown at 16 °C or in total darkness were more active towards the tested strains. While, the EO of plantlets cultured at 35 °C were the least active.

Phenolic profile and bioactivity of the aerial part and roots of Mentha rotundifolia L. grown in two different localities in northeastern Algeria: A comparative study

Mentha rotundifolia L is a valuable plant with an interesting chemical composition. However, the phenolic composition of the Algerian M. rotundifolia was poorly reported. Furthermore, the variation in the phenolic composition of this plant is influenced by many factors that consequently determine its bioactivity. Thus, the present work aimed to assess the effect of provenance and plant organ on the phenolic composition of M. rotundifolia and its bioactivity. Samples were collected in Mila and Jijel and the aerial part and the roots were thus separated. The phenolic contents were determined by colorimetric methods, individual phenolic compounds were identified and quantified by means of LC-ESI-MS, and in vitro assays were employed to assess the bioactivity of the different extracts. The roots of the Mila plant contained the highest total phenolics content with a value of 241.52 ± 2.83μg GAE/mg, while the aerial part of the plant recorded the highest flavonoids (77.026 ± 1.61 μg RE/mg extract) and flavonols (54.85 ± 0.35 μg EQ/mg extract). A total of sixteen phenolic compounds have been identified including phenolic acids and flavonoids. Rosmarinic acid, quinic acid, and salvianolic acid were the main phenolic compounds with the highest amounts (from 3.996 to 125.411 μg/g of extract depending on the plant organ and origin). Most of the compounds present higher levels in the plant from Mila than that of Jijel. Quinic acid was concentrated in the aerial part than in the roots and the inverse for rosmarinic and salvianolic acid. All extracts showed remarkable antioxidant activity, which varied according to the origin and the organ of the plant. The roots of the plant from the Mila region showed the strongest inhibition on ACHE (IC50 = 36.89 ± 0.25 μg/mL) and on α-glucosidase (IC50 = 17.29 ± 0, 09 μg/mL). High UV protection was achieved with SPF values between 35.70 ± 0.07 and 39.49 ± 1.42. These conclude that the soil environmental conditions of Mila are more adequate for polyphenols synthesis and better bioactivity.

Total Phenolic Content and Antimicrobial Activity of Mentha rotundifolia L. from Western Algeria

Total Phenolic Content and Antimicrobial Activity of Mentha rotundifolia L. from Western Algeria

Variability of insecticidal activity of Cupressus sempervirens L., Juniperus phoenicea L., Mentha rotundifolia (L.) Huds, and Asphodelus microcarpus Salzm. & Viv. extracts according to solvents and extraction systems

Medicinal plants represent an enormous potential source of chemical derivatives with many therapeutic properties whose variability is modified by exogenous factors. The present research focused on the evaluation of the effects of solvents with different polarities (hydro-methanolic, hydro-ethanolic, hydro-acetonic, and aqueous) and the extraction methods: maceration extraction (ME), and ultrasound-assisted extraction (UAE) on the total phenolic content (TPC), total flavonoid content (TFC), and total condensed tannins (TCT) in Cupressus sempervirens L., Juniperus phoenicea L., Mentha rotundifolia (L.) Huds, and Asphodelus microcarpus Salzm. & Viv. extracts. The toxicity by contact of the plant extracts obtained against Tribolium castaneum (Herbst) adults was studied. The quantitative characterization revealed significant differences (p ˂0.05) in the secondary metabolites of the different extracts analyzed depending on the method and solvent used. The result of the contact test indicated that the hydro-acetonic extracts obtained by ultrasound-assisted extraction exhibited the strongest insecticidal power with a corrected mortality of 59.25 ± 6.41% and 51.72 ± 11.54% for C. sempervirens L. and J. phoenicea L., respectively, and a mortality rate of 66.66 ± 5.77% was recorded for the hydro-methanolic extract of M. rotundifolia (L.) Huds. While in A. microcarpus Salzm. & Viv., the highest mortality rate (33.33 ± 7.07%) was registered in the hydro-acetonic extract obtained by maceration. The results show that there is a strong relationship between the efficacy of extracts as biopesticides and their abundance in biomolecules.

Evaluation of the insecticidal potential of two medicinal plants and an entomopathogenic fungi against Tribolium confusum Jacquelin du Val. (Coleoptera: Tenebrionidae), a pest of stored foods

Stored products are among the most important foods in human nutrition. However; these products are under the pressure of many harmful organisms.One of these pests is Tribolium confusum Jacquelin du Val. (Coleoptera: Tenebrionidae). In this study, effects of two essential oils (Mentha rotundifolia and Satureja calamintha) and an enthomopathogenenic fungi on Tribolium confusum has been tried under the laboratory conditions, contact and repellent effects of essential oils and contact effect of dry plant extract were determined.As a result, essential oil, 3, 5, 10, 15 and 20 μl / ml doses of Mentha rotundifolia showed 100% effect after 24 hours, while Satureja calamintha reached 100% effect after 96 hours.15 μl / ml dose of M. rotundifolia and 20 μl / ml dose of S. calamintha reached 100% detrimental effect after two hours. Powder doses of 0.6, 0.8 and 1.0 g of these plants, which were applied, caused the death of all the male and female individuals after 24 hours. In addition, the effects of 1x107 conidia / mL dose of the entomopathogenic fungus Beauveria bassiana after 24, 48 and 72 hours were 87.5%, 97.5% and 100%, respectively. It was seen that both essential oils and B. bassiana were successfully in suppressing the depot pest Tribolium confusum.

Identification of volatile compounds and antioxidant, antibacterial, and antifungal properties against drug-resistant microbes of essential oils from the leaves of Mentha rotundifolia var. apodysa Briq. (Lamiaceae)

Abstract The present research work investigated antioxidant, antibacterial, and antifungal properties of essential oils from the leaves of Mentha rotundifolia var. apodysa Briq. (EOR). Hydro-distillation was used to extract EOR before being subjected to the chemical characterization by the use of GC/MS. Antioxidant activity was assessed by the use of three bioassays namely 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and total antioxidant capacity (TAC). Antimicrobial potency was tested against gram-negative and gram-positive bacteria namely Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Acinetobacter baumannii, and Klebsiella pneumonia, while antifungal activity was tested against Aspergillus niger, Candida albicans, Aspergillus flavus, and Fusarium oxysporum. EOR yield was determined to be 1.31%, with 20 compounds wherein Menthol (31.28%) and Isomenthol (14.28%) constituted the greatest amount. Regarding antioxidant activity, EOR exhibited potent antioxidant power: DPPH (IC50 value of 0.36 ± 0.03 mg/mL), FRAP (EC50 value of 0.35 ± 0.03 mg/mL), and TAC (697.45 ± 1.07 mg EAA/g). Antibacterial activity results showed that EOR had broad antibacterial activity on the tested strains. Eventually, EOR resulted in the greatest inhibition zone diameters vs S. aureus (18.20 ± 0.41 mm) followed by E. coli (17.02 ± 0.5 mm). Antifungal activity results showed that EOR exhibited potent antifungal activity and resulted in the greatest inhibition zone diameters up to 51.32 ± 1.32 mm against Aspergillus flavus, and 34.51 ± 1.07 mm against Aspergillus niger.

Ethnobotanical survey of medicinal plant Mentha rotundifolia (L.) Huds. in El Tarf, extreme northeast of Algeria.

Ethnobotanical survey of medicinal plant Mentha rotundifolia (L.) Huds. in El Tarf, extreme northeast of Algeria.

English

This work aimed to assess in vitro insecticidal and antifungal activities of Mentha rotundifolia and Myrtus communis essential oils against the red flour beetle (Tribolium castaneum) and three fungal species (Botrytis cinerea, Fusarium solani and Colletotrichum acutatum). Oxygenated monoterpenes presented the dominant group with 72.94 and 58.92% respectively for M. rotundifolia and M. communis essential oils. M. rotundifolia and M. communis essential oils composition was dominated by 72.94 and 58.92% of oxygenated monoterpenes, respectively. The determined lethal concentrations of mentha essential oils against T. castaneum adults revealed high toxicity respectively for fumigant and contact tests, LC50 = 0.113 μL cm-2 and LC50 = 32.71 μL L-1 air. However, common myrtle oil showed a weak fumigant activity (LC50 = 357.67 μL L-1 air) and no contact toxicity. Furthermore, M. rotundifolia essential oil showed a marked antifungal toxicity against all the fungal strains. The mycelial growth of the three fungal strains was completely inhibited at the concentrations of 0.33 μL L-1 by contact application and 8, 10 and 12 μL by fumigant application. M. communis essential oil displayed only a contact antifungal toxicity against B. cinerea at the concentration 21.33 μL L-1. Additionally, M. rotundifolia completely inhibited conidial germination of B. cinerea and F. solani, and significantly affected their morphology, with morphological modifications at the rate of 92.94 and 51.11% respectively. In light of in vitro tests results, the mentha essential oil appeared to be an excellent source of antifungal and insecticidal components and will allow the potential development of this species in the biological control of several pests and fungal diseases. © 2021 Friends Science Publishers

Antiradical, Antimicrobial effect and Chemical composition of essential oil of mentha rotundifolia. L from mountains el Hamdania, north of Algeria

This work is part of the valuation of essential oil of Mentha Rotundifolia harvested in the mountains of El Hamdania, in the Atlas Blideen (northern Algeria). The essential oil of Mentha rotundifolia extracted by hydro distillation have been analysed by means of Gas Chromatography GC-FID and GC-FID coupled to a Mass Spectrometer GC-MS in combination with retention indices. 34 constituents were identified accounting for 92 % of the essential oil. The main components were piperitenone oxide (30.37%) and piperitone oxide (23.78%). The antimicrobial activity evaluated on seven microbial strains revealed sensitivity for the majority of the bacteria except pseudomonas aeruginosa . The antioxidant activity was determined according to the ability of the tested samples to scavenge the free radicals 2,2-diphenyl-1-picrylhydrazyl (DPPH*). The essential oil were slightly active 21.8% comparing with Quercetin (98.2%) of these oils can be attributed to the absence of some phenolic components, which may play an important role. The inhibitory effect of this essential oil on the development of bacteria and more particularly its effect on staphylococcus aureus sees glimpses of applications in the pharmaceutical industry as a natural antibiotic, and for other applications in the food and cosmetic industries.

Evaluation of Antimicrobial Potential and Comparison of HPLC Composition, Secondary Metabolites Count, and Antioxidant Activity of Mentha rotundifolia and Mentha pulegium Extracts.

In the present study, the relationship between the phenolic counts, chemical composition, and biological activities of two Mentha species (Mentha rotundifolia (MR) and Mentha pulegium (MP)) was analyzed. The characterization of the action mode against pathogenic bacteria and the inhibition of spore germination of two fungal species using prepared methanolic extracts were studied here for the first time. The obtained data highlighted the presence of positive correlation between the secondary metabolites contents and the biological activities of the investigated extracts. In fact, HPLC analysis showed that the major components in both the extracts were eriocitrin and rosmarinic acid (25 and 20 mg/ml and 12 and 8 mg/ml in methanolic extracts of MR and MP, respectively). Moreover, the MR extract was rich in polyphenols and presents the highest antioxidant activity than MP ones. In addition, both extracts possess an antimicrobial activity against four Gram-positive and five Gram-negative bacteria and one yeast species (Candida albicans) and were able to inhibit the spore germination of two fungi species (Aspergillus niger and Aspergillus flavus). But, the significant activity was observed in the presence of MR methanolic extract. The effect of time on cell integrity of E. coli and L. monocytogenes determined by time-kill and bacteriolysis assays showed that the MR extract had a rapid bacteriolytic effect compared to the MP extract, and their capacities were significant against Gram-negative bacteria than positive ones. Based on the obtained data, it can be concluded that Saudi Mentha species have high pharmacological and industrial importance and they can be used in preparation of food or drugs.

Examination of Spectral Properties of Medicinal Plant Leaves Grown in Different Lighting Conditions Based on Mint Cultivation.

Cultivation in controlled environmental conditions can provide good quality medicinal herbs with consistent properties. A sensing system that can determine the contents of medicinal substances in plants using spectral characteristics of leaves would be a valuable tool. Viability of such sensing approach for mint had to be confirmed experimentally, as no data correlating contents of medicinal substances with spectral characteristics of leaves are available, to the best of authors’ knowledge. In the first stage, presented in this paper, the influence of lighting on mint (Mentha rotundifolia) grown on a small hydroponic plantation was studied. Spectral characteristics of leaves were recorded by a spectrophotometer and colorimetric analysis was used to investigate the relationship between these characteristics and the spectrum of lighting. Dry mass yield was measured to test its dependence on the lighting. Dependence of chromaticity of leaves on the spectrum of light used in the cultivation was confirmed. Averaged spectra of leaves are distinguishable using a spectrophotometer and—in most cases—by a human observer. A partial correlation is observed between dry mass yield and the spectrum of lighting. Obtained results justify further research into the correlation between lighting and the contents of biological substances in medicinal plants using spectral characteristics of leaves.