Plant Mentha Research Articles

Gas exchange and chlorophyll fluorescence in spearmint (Mentha spicata L.) leaves influenced by mineral nutrition

The production of export-quality spearmint is limited in Colombia because of low production volumes, poor compliance with good agricultural practices, nutrient availability, and fertilization management. This study aimed to identify how NPK fertilization influences photosynthesis and photochemistry in Mentha plants during vegetative growth in a mesh house. Increasing doses of chemical fertilization were evaluated with a 10-30-10 (N-P-K) formula at 0, 60, 90, 120, and 180 kg ha-1. The evaluated variables were net photosynthesis (A), transpiration (E), stomatal conductance (gs), leaf temperature (Tleaf), quantum yield (Qy), Non-photochemical quenching (NPQ), photochemical quenching (qP), and dry matter (Dm). The highest A, Qy, E, and gs values were in the plants treated with high NPK doses; the NPQ and qP increased in the plants with low NPK doses. These findings elucidated the influence of NPK on photosynthesis and other physiological parameters in the growth and development of spearmint.

Ethnobotanical survey of medicinal plant Mentha rotundifolia (L.) Huds. in El Tarf, extreme northeast of Algeria.

Ethnobotanical survey of medicinal plant Mentha rotundifolia (L.) Huds. in El Tarf, extreme northeast of Algeria.

Exogenous triacontanol provides tolerance against arsenic-induced toxicity by scavenging ROS and improving morphology and physiological activities of Mentha arvensis L.

Arsenic (As), recognized as a toxic metalloid globally, has posed a serious threat to soil, plants and aquatic resources. Arsenic restrain fundamental processes of plant grown under the As-contaminated soil which are the worst sufferers for their sustenance. Thus, various scientific strategies are being continuously employed for reducing the soil As. In this regard, use of well-known plant growth regulators (PGRs) like triacontanol (TRIA) shown great efficiency towards heavy metals stress tolerance. TRIA is a plant growth promoter that has been proved effective for growth and development of plants under diverse environmental conditions for many decades. The research work was carried out to examine the toxic effects of As on various morphological, physio-biochemical, yield and quality parameters of Mentha arvensis L. and amelioration of the As toxicity through exogenous application of TRIA. Mentha plants were supplemented with various treatments (i) 0 (control), (ii) TRIA (10−6 M), (iii) As (60 mg kg−1), (iv) As (80 mg kg−1), (v) TRIA (10−6 M) + As (60 mg kg−1), and (vi) TRIA (10−6 M) + As (80 mg kg−1). Several parameters studied during the present investigation were plant height, fresh and dry weights, herbage yield, chlorophyll and carotenoid content, carbonic anhydrase (CA) activity, several chlorophyll fluorescence parameters, superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), proline (PRO), H2O2 content, TBARS content, electrolyte leakage (EL), essential oil content (EO) and yield and microscopic analyses as well as PCA analysis. Arsenic treatment exhibited deleterious effects on the overall growth, photosynthetic, and quality parameters of M. arvensis. However, the toxicity of As was mitigated by the leaf-applied TRIA to the plants, proved advantageous in combating the ROS generation.

Green Synthesis of Antibacterial Silver Nanoparticles against Drug Resistant Bacteria

Background: Nanotechnology brings a massive revolution in the domain of medical biotechnology. Plants possess thousands of antimicrobial compounds; these compounds can be extracted and utilized in the production of medicines. Objective: The aim of this research is to design an economical method for the formation of nanoparticles from plants and explore their antibacterial activity. Methods: In the present study, we used the plants Aloe barbadensis, Azadirachta indica, Carica papaya, Mentha arvensis and Catharanthus roseus. Ethanolic, methanolic and aqueous extracts of the above mentioned plants were used to prepare silver nanoparticles. Antimicrobial activities of the nanoparticles of extracts were determined against pathogenic strains of bacteria. Results: Results showed that silver nanoparticles are formed from the ethanolic and methanolic extracts of Azadirachta indica, Carica papaya, Mentha arvensis and Catharanthus roseus and from the aqueous extracts of Aloe barbadensis and Mentha arvensis. It was observed that nanonoparticles of ethanolic extracts of Azadirachta indica, Carica papaya, Mentha arvensis and Catharanthus roseus exhibited the highest antibacterial activity against Pseudomonas specie, E.coli, Pseudomonas specie and S.typhi, respectively. On the other hand, the nanoparticles of methanolic extracts of Carica papaya, Mentha arvensis and Catharanthus roseus showed the highest inhibition against E.coli, whereas, maximum zone of inhibition of Pseudomonas was observed by the action of methanolic extract of Azadirachta indica. It is also found that nanoparticles of the aqueous extracts of Aloe barbadensis and Mentha arvensis inhibited the pathogenic strains and showed maximum activity against Pseudomonas and E.coli, respectively. Conclusion: The above results indicate that the nanoparticles formed from the extracts of the tested plants have high antimicrobial potential and could be utilized in the preparation of medicines to treat various disorders.

Stemphylium and Ulocladium between Benefit and Harmful

Background: Emerging of microbial resistance, spread of life-threatening diseases, and biological control of pathogens destroying economically important crops, are serious problems that encourage scientists to search for unusual sources for novel compounds with biological activities. Fungi are promising sources for such compounds due to their ability to produce variety of secondary metabolites that could be, if truly investigated, the solution for currently serious problems. Aim: The aim of this review is to highlight the diversity of compounds produced by endophytic Stemphylium and Ulocladium and represents their ability to produce biologically diverse metabolites. Materials and methods: This was a narrative review. A comprehensive literature search was done using PubMed, Google Scholar, Scopus, and EMBASE using the keywords, Stemphylium; Ulocladium; Secondary metabolites; biological activities. Results: Many studies reported that the endophytic Ulocladium especially, Ulocladium atrum Preuss, showed promising biocontrol activity against Botrytis cinerea on crops cultivated in the greenhouse and the field. The endophytic fungus Stemphylium especially, Stemphylium globuliferum was isolated from stem tissues of the Moroccan medicinal plant Mentha pulegium. Extracts of the fungus exhibited significant cytotoxicity when tested in vitro against L5178Y cells. Conclusion: Endophytic fungi are a noble and consistent source of unique natural mixtures with a high level of biodiversity and may also yield several compounds of pharmaceutical significance, which is currently attracting scientific surveys worldwide. Every study conducted on Stemphylium and Ulocladium resulted in discovery of new metabolites or pointing to a possible application, which made Stemphylium and Ulocladium species potential source of pharmaceuticals and attracted attention for further investigations of their biological control.

Incorporation of biogenic zinc nanoparticles into a polymeric membrane: Impact on the capture of organic herbicides

Nanoparticle incorporated membranes are potential tools to remove hazardous organic pollutants from drinking water sources. Cellulose acetate membranes were impregnated by incorporating zinc nanoparticles, produced using a green synthesis with Mentha plant extract utilizing microwave energy. The synthesized nanoparticles were extensively characterized using ultraviolet–visible spectroscopy, X-ray diffraction, Scanning electron microscopy, energy dispersive X-ray spectroscopy and Nanoparticle tracking analysis. The spectroscopy results showed an absorbance peak at 300 nm, confirming the successful synthesis, while X-ray diffraction confirmed that the particles are crystalline with a mean particle size of 36 nm. The surface roughness and topography of the zinc nanoparticle impregnated membranes was studied using atomic force microscopy to check the homogeneity of dispersion of the nanoparticles in the membrane. The efficiency of the zinc nanoparticles and the zinc nanoparticle loaded cellulose acetate membranes for adsorption of organic herbicides such as metolachlor and acetochlor was studied. The particles themselves and the particle-embedded membranes proved to be very adsorbent due to their unique structural properties, providing large surface area and high adsorption capacity. The incorporation of zinc nanoparticles into the membrane decreased the time needed for the removal of the selected herbicides relative to that of the nanoparticles alone, and provides a promising method for water purification. • Zero valent Zinc NPs (36 nm, 98.34% Zn) are easily synthesized using a green method. • ZnNPs adsorbed model pesticides acetochlor (61%) and metolachlor (97%) in 60 min. • Incorporation of ZnNPs in a cellulose membrane increased their adsorption ability. • The ZnNPs-CA complex removed 99.8% metolachlor in 20 min & 61% acetochlor in 30 min. • Mineralization of herbicides and NP stability were enhanced by the membrane support.

Intercropping With Peppermint Increases Ground Dwelling Insect and Pollinator Abundance and Decreases Drosophila suzukii in Fruit

Intercropping can be used to reduce pest insects within agricultural systems, e.g., through deterring pests directly or by increasing habitat for their natural enemies. For example, plant produced volatile organic compounds (VOCs) can deter or confuse host-finding by insects through olfactory disruption. Drosophila suzukii is an invasive fruit fly of agricultural concern as it can lay its eggs in both ripening and fresh fruits and, uses olfactory cues to identify its wide range of host plants. Peppermint plants (Mentha × piperita) produce high levels of VOCs while growing and may, therefore, be suitable as an intercrop to reduce D. suzukii infestations in the field, as peppermint essential oil VOCs have previously been shown to deter D. suzukii in olfactory trials. We conducted a field intercropping experiment to evaluate the effectiveness of peppermint plants compared to traditional ryegrass/clover mixes in reducing D. suzukii oviposition in the field, and the effect of peppermint intercrops on other invertebrates. In the field, we monitored sentinel fruit baits weekly for D. suzukii infestation. Additionally, we monitored intercropping effects on the invertebrate community through weekly pitfall trap collection and through a pollinator point survey. We monitored for local, farm level presence of D. suzukii through apple cider vinegar traps within crop fields and along hedgerows and found high abundance of D. suzukii (>3,000 individuals trapped). Peppermint intercrops had fewer D. suzukii emerge from fruit baits and supported greater beneficial insect abundance (predators and pollinators) compared to ryegrass/clover. However, levels of D. suzukii were low across both intercrop types. Overall, we found that peppermint intercrops could be a potential aromatic intercrop used to reduce D. suzukii adult emergence from fruit compared to conventional ryegrass/clover mixes, however this trial should be replicated over multiple growing seasons, geographic locations, and host fruits. Furthermore, further study should determine the effects of the intercrop on the focal crop of interest.

Effect of Topical Products Extracted from Essential Oils of Capsicum, Apium graveolens and Mentha Plants on the Pain Intensity in Patients with Inflammatory Pain

Background: Inflammatory pain is the spontaneous hypersensitivity to pain that occurs in response to tissue damage and inflammation. Recently herbal remedies have been considered for less complications. The two topical products of Capsicum (Capex Topical cream) Apium graveolens and Mentha (CM lotion) can reduce the inflammatory. Since Capex Topical cream causes skin irritation, it seems that its composition with the other product could probably reduce this complication. Methods: This experimental study was performed on 60 patients referred to orthopedic clinic of Shahid Yahyanejad Hospital, Babol in 2018. Patients were randomly divided into three treatment groups. Data collection was done by a researcher-made questionnaire, which included two parts of individual characteristics and the severity of pain. The severity of pain was measured by visual analogue scale (VAS) before intervention and two and four weeks after the intervention. Data were analyzed using SPSS-16 software and P < 0.05 was considered as significant. Results: The results showed that the intensity of pain after use of each of the products decreased in all three groups. In addition, our results demonstrated that application of Capex Topical Cream was more effective that use of CM lotion in decreasing the pain intensity (P < 0.05). Moreover, the intensity of pain after topical application of the combined lotion of CM and Capex Topical cream significantly decreased (P < 0.05), however the decrease in pain intensity using combined solution was not significant compared to application of Capex Topical cream or CM lotion alone. Conclusions: The present study showed that the use of all topical products reduces the severity of pain and due to the less side effects and complications such as skin irritation in application of combined solution and better acceptance by patients, it could be suggested the use of combined solution as a better alternative to reduce inflammatory pains.

Impact of Different Crystal Sizes of Nano-Iron Oxide as Fertilizer on Wheat Plants Photosynthetic Pigments Content.

This study was carried out to investigate the impact of Fe2O3 NPs synthesized from mentha, onion and moringa plant extract at the concentrations of 20,40 and 60 and 80 ppm on wheat plant as fertilizer. Plant pigments chlorophyll a, b carotenoids as well as total pigments contents were investigated. Also, total phenolic components contents in wheat shoots were determined. The results showed that the foliar application of iron oxide nanoparticles at low concentrations (20,40 and 60 ppm) induced an increment in photosynthetic pigments and total phenolic compounds contents. beyond these concentrations an inhibitory effect was shown at 80 ppm concentration. Different sizes of nanoparticles showed different impacts on the studied criteria, small particle size induced more enhancement in plant pigments and phenols contents while larger sizes showed less impact.

Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase

Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha.

Investigation of Genetic Diversity of Native Ecotypes of Northwestern Medicinal Plant Mentha longifolia using ISSR Molecular Markers

Investigation of Genetic Diversity of Native Ecotypes of Northwestern Medicinal Plant Mentha longifolia using ISSR Molecular Markers

Impact of Foliar Application of Amino Acids on Total Phenols, Phenolic Acids Content of Different Mints Varieties under the Field Condition.

Phenolic compounds have a number of benefits to human health and can be used as preventive compounds for the development of some chronic diseases. Mentha plants are not only a good source of essential oils, but also contain significant levels of wide range of phenolic compounds. The aim of this research was to investigate the possibility to increase phenols content in Mentha plants under the foliar application with L-phenylalanine, L-tryptophan, L-tyrosine at two concentrations (100 mg L−1 and 200 mg L−1) and to create preconditions for using this plant for even more diverse purposes. Quantitative and qualitative analyses of phenols in mints were performed by HPLC method. Foliar application of amino acids increased the total phenol content from 1.22 to 3.51 times depending on the treatment and mint variety. The most pronounced foliar application to total phenols content was tryptophane especially in Mentha piperita “Swiss”. Mentha piperita “Swiss” was affected most by foliar application and the amount of total phenolic acids depending on the treatment ranged from 159.25 to 664.03 mg 100 g−1 (DW), respectively, non-sprayed and sprayed with tryptophane 100 mg L−1. Our results suggest that the biophenol content varies according to such factors as foliar application and variety, and every single mint variety has individual response to different applications of amino acids.

Development of adsorbent from Mentha plant ash and its application in fluoride adsorption from aqueous solution: a mechanism, isotherm, thermodynamic, and kinetics studies

In the present study, Mentha plant ash was modified by Na and Al for the synthesis of adsorbent and applied for the removal of Fluoride from an aqueous solution. Mixture of acid washed Mentha plant ash (MPA) and NaOH (in the ratio 1:1.3) thermally treated at 600°C in a muffle furnace then treated with aqueous solution of sodium aluminate. The characterization of sodium aluminum modified ash (Na-Al-MA) powder was done such as SEM (Scanning Electron Microscopy), Particle Size Analysis (PSA), Fourier transformed spectroscopy (FTIR), Zeta Potential, XRD (X-ray Diffraction) analysis, and Brunauer–Emmett–Teller (BET) analysis. The removal of fluoride from an aqueous solution carried out with Na-Al-MA by batch adsorption process. The Na-Al-MA was found to be very effective as adsorbent. The maximum removal of fluoride was achieved ̴ 86% at neutral pH and at room temperature. It was investigated that Langmuir adsorption isotherm and pseudo-second-order kinetic was best fitted for fluoride adsorption. The fluoride adsorption on Na-Al-MA was an exothermic process. A possible mechanism including electrostatic attraction, hydrogen bonding, and metal-fluoride interaction for fluoride adsorption on Na-Al-MA have described in this study. Novelty statement: Utilization of Mentha plant ash for the development of adsorbent and its application in adsorptive removal of fluoride from aqueous solution is the novelty of this work. Adsorbent preparation may be the better way of waste biomass management.

Chemical composition and insecticidal, repellent and antifungal activities of essential oil of Mentha rotundifolia (L.) from Algeria

The biological struggle by the use of the natural substances that have a pesticide effect may be an alternative to harmful chemicals; among these natural substances are the extracts of aromatic plants especially essential oils. The present work aims to highlight the fungicidal and insecticidal effect of the essential oil (EO), of an aromatic plant Mentha rotundifolia, against respectively, a phytopathogenic mold (Fusarium culmorum) and the adults of two pests (Sitophilus granarius and Tribolium confusum) of stored grains. The evaluation of the chemical composition of the EO was carried out by gas chromatography coupled to mass spectrometry (GC/MS). The analysis made it possible to identify 27 compounds, which corresponds to 97.93 % of the totality of the oil of which the majority compounds are: Piperitenone oxide 35.49 ± 0.08, Caryophyllene oxide 35.27 ± 0.13 and cis-Cinerolone 10.95 ± 0.05. The bioassays to assess the EO toxicity towards the pest insects revealed a very high effect, which increases significantly with the dose and the time of exposure. Similarly, the test for the repellent effect shows that this extract is a powerful insect repellent. In addition, we found that both the liquid and vapor phases of the EO present an inhibitory effect of the growth of the fungal strain. In the light of the results obtained, the plant studied is promising as a source of natural pesticides and lends itself well to research in the field of pest control using biochemical alternatives.

Bioinoculant with Vermicompost Augments Essential Oil Constituents and Antioxidants in Mentha arvensis L.

Today’s demand has approached the sustainable agriculture with synergistic bioinoculants for improved crop production and soil rejuvenation. Furthermore, the emergence of chemical fertilizers, for intensive farming practice increasing for many decades without the knowledge of health hazards and environmental problems, can be resolved. Purposely, the present study was conducted on commercially grown medicinal plant Mentha arvensis L. to improve productivity and yield using natural bio-sources such as bioinoculant and vermicompost. The new findings illustrated that M. arvensis L. showed significantly (P < 0.05) improved plant growth, secondary metabolites, total phytoconstituents, and total antioxidant capacity in bioinoculant-treated plants as compared to the control with carrier vermicompost. Moreover, the highly effective treatment also exceeded the most prominent aroma compounds menthol, menthone, and isomenthone. Most interestingly, with high yield increment, the essential oil also showed significant antibacterial activity. The present study concluded an unambiguous fortification of bioinoculant with carrier vermicompost, showing a promising synergistic potential that validates itself as bio-stimulants in enhancing physiological and biochemical properties of M. arvensis L. resulting in high yield and improved quality.

Production of phenolic acids in hairy root cultures of medicinal plant Mentha spicata L. in response to elicitors.

In this study, hairy root induction in leaf and stem explants of Mentha spicata using various Agrobacterium rhizogenes strains was established for the first time. Although inoculation of explants by immersion method resulted in tissue necrosis, direct injection of explants by all examined strains (A13,R318,A4,GMI 9534 and ATCC15834) was effective. All different parts of the stem were susceptible to A. rhizogenes infection. However, the middle and lower internodes showed a higher rate of transformation. Among the different strains, the strain A13 exhibited the highest infection efficiency (almost 75% of the explants). A13 and R318-infected hairy roots showed the highest biomass production (close to 60 mg/flask), while infection with GMI 9534 produced the highest content of phenolic acids. Finally, the effect of phytohormone elicitation on hairy root growth and phenolic acid biosynthesis was investigated. A substantial increase in root growth and phenolic acids accumulation was obtained followed by 0.3 mg L-1 IBA and 100 µM MeJA treatment, respectively.

Seasonal Variation in the Essential Oil Composition of Mentha aquatica L. and its Myorelaxant Activity

The present investigation was accomplished to study the chemical variability of the essential oil of the plant Mentha aquatica L. collected from Bhimtal, Uttarakhand, India during different seasons ranging from April to December. The essential oils collected at various month intervals were subjected to be analyzed with GC along with MS and FID for their comparative chemical composition analysis. Significant differences in the chemical variability were observed in major components of each collection. Linalool represents the dominating compound in every six collections from April to September comprising a total of 30.1 %, 25.2 %, 33.5 %, 48.4 %, 31.1 % and 45.7 % respectively of total oil composition while linalool acetate represents the dominating compound in next three collections from October to December comprising a total of 46.98 %, 42.1 % and 48.0 % respectively of total oil compositions. Thereafter, the essential oil of the tested plant was subjected to assess the efficiency of myorelaxant activity on the isolated duodenum muscles of Swiss Albino mice. Essential oil at different treatments of 2000 μg and 4000 μg resulted into least to the mild grade of relaxation in the muscles evaluated against the maximum response towards acetylcholine, adrenaline, atropine and propanalol.

Comparison of the properties of essential oils from commercial sources and edible dry mint

Peppermint oil is isolated from the plant Mentha piperita L, which is an aromatic perennial herbs belonging to the family of Lamiaceae found all over the world. Peppermint oils of several mentha species can be used for pharmaceutical and nutritional aspects, as natural additives in medicine, drugs, foods, mouthwash, toothwash, chewing gum and confectionary because of their antiviral, antibacterial, antifungal, pesticidal, anti-inflammatory and antimicrobial propertiesand pain decreasing and immunity increasing activities. In this study is to research the difference of the chemical constitutients of essential oil from some commercial mint oils and edible dry mint with Clevenger method. We have investigated the chemical composition of the mint oil samples with GC/MS and optical rotations were determined to see which chemicals might cause this difference. We have found that the best essential oil with regard to menthol content is the purchased Turkish mint oil sample. Chinese mint oil was the second. Edible mint oil is rich in carvone. The mint essential oil of dry mint leaves obtained with Clevenger method has a high carvone content, so it could be used in aromatherapy and alternative medicine

A Detailed Review on Pharmacological Profile of Mentha piperita

Among the several plants Mentha Piperita Linn commonly known as peppermint is an important medicinal herb that belongs to the family called Laminaceae. Itrsquos one of the ancient medicinal herb or species majorly used in food medicines and cosmetics. The leaf of this drug has been proven useful in relief of common cold decreases the symptoms of irritable bowel syndrome digestive problems such as nausea vomiting diarrhoea flatulence and dyspepsia. The herb is used as analgesic topically and helps to treat the minor headache. M. Piperita is in the Food and Drug Administrationrsquos FDA Generally Recognized as Safe GRAS list but this herb has its own few side effects. The peppermint oil leads to burning sensation in heart or irritation in perianal region so that itrsquos contraindicated in patients who suffer from bile duct obstruction inflammation in gall bladder and sever damage in liver and precaution should be taken in patients with gastrointestinal tract GIT reflex. Menthol and their derivatives are not used directly under the nose of small children and infants due to the risk of apnea.Keywords Mentha Piperita Peppermint Phytoconstituents Medicinal benefits Pharmacological actions

Economic valuable traits of promising breeding samples and ‘Chornolysta’ variety of Mentha piperita L. after in vitro sanitation and micropropagation

Purpose. To study the impact of clonal micropropagation and sanitation in vitro by viricide Ribavirin on ex vitro plant productivity, quantitative content and qualitative composition of peppermint essential oil components obtained from four breeding samples of peppermint plants (Mentha piperita L.) and the ‘Chornolysta’ variety. Methods . The study used methods of field agrotechnical one-factor experiment, essential oil distillation with water vapor according to the Ginzberg method, capillary gas chromatography and statistical analysis. Results. Due to the sanitation process in vitro, the yield of air-dried leaves of breeding samples increased by 2.9–7.1%, the ‘Chornolysta’ variety by 51.4% and rhizomes by 2.2–3.8% and 28.5% respectively, which amounted 0.35–2.74 t/ha. A significant increase of essential oil yield of breeding samples from 4.0 to 9.9 kg/ha was shown, and of the ‘Chornolysta’ variety – up to 28.6 kg/ha. After in vitro sanitation and clonal cropropagation of the breeding sample M 01-12, the content of essential oil was more than 4%. The following components of peppermint essential oil were identified: limonene, cineole, menton, mentofuran, isomenton, menthyl acetate, β-caryophyllene, isomenthol, menthol, pulegon, piperitone and carvone. A clear tendency to a decrease in the amount of menton and isomenthol with isomenton and menthol increase in plants, sanitated and propagated in vitro, was revealed. Conclusions . The use of tissue and organ culture methods and in vitro sanitation improves the qualitative composition of terpenoids by increasing the amount of menthol and menton reducing. The data obtained on the composition of terpenoids should be considered in peppermint selection as one of the integral criteria, which should be included in the list of economically valuable characteristics of peppermint plants, such as foliage, biomass of air-dried leaves, plant rhizomes and the amount of peppermint essential oil. Six indicators of the essential oil of the breeding sample M 01-02, namely citric acid, cineole, mentofuran, isomentone, pulegon, carvone, as well as the cineole / limonene ratio, meet the criteria of the European Pharmacopoeia, so it can be considered as promising for cultivation among the studied samples.