Mentha Arvensis Research Articles

A Study on the Stability and Antimicrobial Efficacy of a Newly Modeled Teat Dip Solution Containing Chlorhexidine.

Despite much focus on mastitis as an endemic disease, clinical and subclinical mastitis remains an important problem for many herds. Reducing the usage of antibiotics for mastitis treatment allows the risks to be minimized related to the development of antimicrobial resistance and the excretion of antibiotics into the environment. The aim of the study was to determine the physico-chemical properties, stability and antimicrobial effect of a newly formulated biocide for post-milking udder hygiene containing a thickener made from hydroxypropyl guar gum, an antiseptic chlorhexidine digluconate and teat skin-friendly components including glycerol, Mentha Arvensis herbal oil and Aesculus hippocastanum extract. Hydroxypropyl guar gum was used as a thickener to provide the physical parameters and to retain the viscosity at 1438 mPa.s. The physical and chemical properties of the product, including the 12-month stability, were tested in long-term and accelerated stability studies. The product was effective against the primary mastitis pathogens, including Staphylococcus aureus, Streptococcus uberis, Escherichia coli, Candida albicans and Aspergillus niger.

Silicon dioxide nanoparticles suppress copper toxicity in Mentha arvensis L. by adjusting ROS homeostasis and antioxidant defense system and improving essential oil production

Copper (Cu) is an essential micronutrient for plants; however, the excessive accumulation of Cu due to various anthropogenic activities generates progressive pollution of agricultural land and that causes a major constraint for crop production. Excess Cu (80 mg kg−1) in the soil diminished growth and biomass, photosynthetic efficiency and essential oil (EO) content in Mentha arvensis L., while amplifying the antioxidant enzyme's function and reactive oxygen species (ROS) production. Therefore, there is a pressing need to explore effective approaches to overcome Cu toxicity in M. arvensis plants. Thus, the present study unveils the potential of foliar supplementation of two distinct forms of silicon dioxide nanoparticles (SiO2 NPs) i.e., Aerosil 200F and Aerosil 300 to confer Cu stress tolerance attributes to M. arvensis. The experiment demonstrated that applied forms of SiO2 NPs (120 mg L−1), enhanced plants' growth and augmented the photosynthetic efficiency along with the activities of CA (carbonic anhydrase) and NR (nitrate reductase), however, the effects were more accentuated by Aerosil 200F application. Supplementation of SiO2 NPs also exhibited a beneficial effect on the antioxidant machinery of Cu-disturbed plants by raising the level of proline and total phenol as well as the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutathione reductase (GR), thereby lowering ROS and electrolytic leakage (EL). Interestingly, SiO2 NPs supplementation upscaled EO production in Cu-stressed plants with more pronounced effects received in the case of Aerosil 200F over Aerosil 300. We concluded that the nano form (Aerosil 200F) of SiO2 proved to be the best in improving the Cu-stress tolerance in plants.

Ayurvedic Herbs Advised for COVID-19 Management: Therapeutic Potential and Clinical Relevance

Abstract: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide. There is no effective medication for COVID- 19 as of now, so it would be good to take preventive measures that not only boost our immunity but also fight against infections. The use of traditional Chinese medicine in China to treat COVID-19 patients sets the prototype demonstrating that traditional medicines can contribute to prevention and treatment successfully. In India, the Ministry of AYUSH (Ayurveda, Yoga, Unani, Siddha, Homeopathy) released a self-care advisory during the COVID-19 crisis as a preventive aspect. This review article discusses the therapeutic potential and clinical relevance of some herbs [(Tulsi (Ocimum sanctum), Haridra (Curcuma longa), Tvaka (Cinnamon), Maricha (Piper longum), Shunthi (Zingiber officinale), Munakka (Dried grapes), Lavang (Syzigiumaromaticum), Pudina (Mentha arvensis), and Ajwain (Trachyspermum ammi)] advised by AUYSH to take during COVID-19 infection. They are effective in COVID-19 management, therefore, authors have discussed their detailed traditional uses as therapeutics and spotted scientific insight and clinical significance of the herbs mentioned above along with their mechanistic viewpoint, adequately, on a single platform. Provided information could be a treasure to open up a new research arena on natural products to manage human health crises effectively, caused not only by COVID-19 but also by other infectious diseases.

The potential of Medicinal and Aromatic Plants cultivation to develop the Agri-business in Mizoram

India is one of country which is known for its agricultural products and currently more than 70% population still living in rural area. India is fully blessed with different kinds of agro-climatic regions. Production wise our country is standing at second rank throughout the world. Some of agricultural products from India are very high in demand in many parts of the globe. Counting its agro-climatic regions, the North East Region (NER) is another hot spot acquired a land of agriculture as well as forest area. The each states of NER having its own peculiarity for various agro products. They used to cultivate many crops which include export level to their daily delicacy. Ginger, Turmeric, Chili, Beans and bamboo products are produced in large quantities. As per the statistics, Mizoram is the highest ginger producing state (8.4 tons/hec) followed by Arunachal Pradesh (7.9 tons/hec), Assam and Nagaland (6.99 tons/hec) among the states of NER. Apart from cultivation, 60% area of NER comes under forest cover, especially Mizoram, where more than 80% of the geographical area of the state is under forest and tree cover. Looking into the prospects of diverse agricultural land settings and forest cover including biodiversity of medicinal and aromatic plants (MAPs), Mizoram could be potential state in relation to agribusiness. There is a tremendous scope for MAPs sector in the state. Valuable MAPs like Homalomena aromatica, Alpinia galanga, Cinnamomum tamala, C. verum, Mentha arvensis, Artemisia pollens, Ocimum sanctum, Elsholtzia communis and many others are traditionally cultivated on very small scale. Thus, there is a need to introduce good agricultural practices (GAPs) for increasing the raw materials/yield to establish the agri-market in Mizoram.

Cytotoxic Screening and Enhanced Anticancer Activity of Lippia alba and Clinopodium nepeta Essential Oils-Loaded Biocompatible Lipid Nanoparticles against Lung and Colon Cancer Cells.

Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0-500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145-275 µL/L). The gas chromatography-mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140-150 nm, narrow size dispersion, and Z potential around -5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO.

Mint Plants (Mentha) as a Promising Source of Biologically Active Substances to Combat Hidden Hunger

Hidden hunger, also known as micronutrient deficiency, is a form of undernutrition, which is exacerbated when food security is fragile. However, the amount of phytonutrients in the diet can be increased by using underutilized species, such as fresh mint greens (Mentha). These plants have a high biological value due to the high content of biologically active substances. Plants of the genus Mentha, however, differ significantly in their chemical composition and, thus, nutritional value. The main objective of this study was to evaluate the content of phytonutrients in different Ukrainian species of mint: peppermint (Mentha piperita), horsemint (Mentha longifolia L.), silver-leaved horsemint (Mentha longifolia S.), and spearmint (Mentha spicata L.), and to determine their stability after drying and freezing. After studying the chemical composition of fresh, dried, and frozen plants of these species, it was established that Ukrainian mint species offer a robust set of phytonutrients and can be used as ingredients of the so-called “functional foods.” The biologically active substances in mint are concentrated during drying. Moreover, such raw materials are easily stored and used as an ingredient. However, the losses of ascorbic acid due to drying mint reach 70%; of carotenoids—approximately 10–15%; of chlorophylls—21–38%; and of phenols—19–29%. Peppermint and spearmint were observed to have higher stability of biologically active compounds. As for the freezing, the best stability of the chemical composition was demonstrated by field mint and spearmint samples. Therefore, a targeted selection of mint types and varieties prior to processing will allow preserving maximal preservation of a maximum amount of biologically active substances, increasing the content of phytonutrients in finished products, and preventing the development of hidden hunger.

Exploring the antibacterial, antidiabetic, and anticancer potential of Mentha arvensis extract through in-silico and in-vitro analysis

BackgroundMentha arvensis has been utilized in diverse traditional medicines as an antidiabetic, anticarcinogenic, antiallergic, antifungal, and antibacterial agent. In this work, we have explored the phytochemical analyses and pharmacological potential of Mentha arvensis using both in silico and in vitro approaches for drug discovery.MethodsTo determine the extract with the highest potential for powerful bioactivity, ethanol was used as the solvent. The phytochemical components of the extracts were quantified using liquid chromatography-mass spectrometry analysis. The potential bioactivities of extracts and lead phytocompounds, including their antibacterial, cytotoxic, and anti-diabetic effects, were evaluated.ResultsThe compounds oleanolic acid, rosmarinic acid, luteolin, isoorientin, and ursolic acid have been identified through liquid chromatography mass spectrometry analysis. Based on antimicrobial research, it has been found that the Mentha arvensis extract shows potential activity against K. pneumoniae which was 13.39 ± 0.16. Mentha arvensis has demonstrated a greater degree of efficacy in inhibiting α-glucosidase, with an inhibition rate of 58.36 ± 0.12, and in inhibiting α-amylase, with an inhibition rate of 42.18 ± 0.83. The growth of HepG2 cells was observed to be significantly suppressed upon treatment with extracts obtained from Mentha arvensis. Finally, In-silico methods demonstrated that the Luteolin and Rosmarinic acid exhibit acceptable drug-like characteristics. Furthermore, Molecular docking studies further demonstrated that both compounds have strong potential to inhibit the active sites of therapeutically relevant enzymes involved in Diabetes, Bacterial infections, and Cancer.ConclusionsThe results of this study suggest that the Mentha arvensis extract possesses potent pharmacological potentials, particularly in terms of antibacterial, anti-diabetic, and cytotoxic effects. Particularly, Luteolin and Rosmarinic acid were identified as the top contenders for potential bioactivity with acceptable drug-like properties.

ESSENTIAL OIL IN THE MANAGEMENT OF THE SEED BANK OF INFESTANT PLANTS

The weed seed bank causes damage to agriculture, due to competition with cultivated plants and raising production costs, Cenchrus echinatus has a high seed multiplication and dissemination mechanism and Conyza bonariensis glyphosate resistance mechanism. The objective was to evaluate the potential of Mentha arvensis essential oil by means of fumigation in the weed seed bank. Soil samples (2 kg per treatment) were collected, homogenized and subjected to fumigation for 24 and 48 hours at concentrations of 2.5% and 5.0% of essential oil. The soil was solarized (control) and dried in the shade for three days as a witness. Then, 500 g of soil were distributed in four trays in a completely randomized design, where they received irrigation, identification and registration of emerging seedlings. Data were checked for normality and homogeneity of variance, transformed when necessary and submitted to analysis of variance at a 5% significance level. The averages were performed by Tukey and Dunnet tests at 5% probability. It was concluded that fumigation reduced grass and horseweed emergence regardless of dose and exposure time, with results similar to solarization.

Mint leaves (Mentha arvensis) mediated CaO nanoparticles in dye degradation and their role in anti-inflammatory, anti-cancer properties

In ancient times, herbal plants were considered one of the greatest gifts from nature that human beings could receive, and about 80% of these plants have medicinal uses. In traditional medicine, Mentha arvensis, commonly known as mint, has many applications, and in the present study, the mint leaf extract has been used to synthesis nanoparticles using the mint leaf extract as a biosource for the extraction of nanoparticles. In addition to having a wide range of applications in various fields, calcium oxide (CaO) nanoparticles are also considered to be safe for human use. In order to assess the characteristics of the abstracted CaO nanoparticles, UV–visible absorption spectrophotometers, Fourier Transform Infrared spectrophotometers (FTIR), Scanning Electron Microscopes (SEMs), Dynamic Light Scattering (DLS), and X-ray Diffraction Spectrophotometers (XRDs) were used. By conducting a protein denaturation assay and nitric oxide scavenging assay, mint leaf mediated CaO nanoparticles were evaluated for their therapeutic applications. MTT assays were used to prove that the CaO nanoparticles mediated by mint leaf had anti-cancer properties. By examining the ability of mint leaf mediated CaO nanoparticles to degrade various dyes such as methyl red, methyl orange, and methylene blue, which are the most used azo dyes in textile industries resulting in water contamination, the ability of these nanoparticles to act as a photocatalytic agent was examined.

Biorational potential of Mentha essential oils of Indian origin: Comparative note on insecticidal efficacy, fumigant persistence, oviposition deterrence of Sitophilus oryzae

Natural compounds have widely gained their acceptance as biorationals for management of storing grain from insect pests. Mentha spp. are commercially important plant genus known for its distinct aroma. The present study is aimed towards the comparative insecticidal activity of four mint essential oils (Mentha arvensis, Mentha citrata, Mentha piperita and Mentha spicata) against rice weevil (Sitophilus oryzae). Further, the GC-MS analysis of mint essential oil’s (EO’s) exhibited diversity in aroma compounds, M. arvensis (menthol 61% and l-menthone 9.11%), M. citrata (linalool 36.74% and linalyl acetate 35.9%), M. piperita (menthol 36.28%, menthone 26.37% and iso-menthone 5.15%) and M. spicata (54.14% of carvone and 20.97% of limonene). Whereas, the M. spicata EO exhibited the strongest toxicity (fumigant and contact) in both with and without food conditions around 100% mortality for 25 μL dose within 24 h of exposure. The LC50 and LD50 values exhibited by M. spicata were 54.07 μL/L and 11.5 μL/100 cm2 respectively. Similarly, the concentrations of 2.5% and 5% of M. spicata EO also exhibited a strong fumigant and contact toxicity against S. oryzae within 24 h as compared to known prophylactic insecticide (0.3% of Deltamethrin). Besides, 100% oviposition deterrence exhibited by all the four mint EO’s and 56% of AChE inhibition is noticed for M. spicata EO. Furthermore, post-treated grains showed no persistence of mint aroma residues and the treated grains retained the seed viability. Thus, on a comparative note, the M. spicata EO possess potential biorational traits for the effective management of stored grain from insect pests.

Growth, oil and physiological parameters of three mint species grown under saline stress levels.

Salinity stress is known to have a detrimental effect on mint plants. The aim of the present work was to investigate the possible effects of salinity stress on Menthaspicata, Mentha piperita and Mentha arvensis. Plants were exposed to salinity stress using different concentrations of NaCl (0, 50, 100, 150 mM). Under salinity stress, plant growth, oil yield, content and composition, as well as physiological parameters were adversely affected. Among the studied species, M. arvensis experienced the maximum loss in terms of oil percentage. Physiological characteristics and oil composition were significantly affected with intensification of salt stress. For instance, in M. spicata, with increasing salinity stress, piperitone oxide was decreased from 78.4% in control to 38.0% in 150 mM NaCl, whereas menthol was increased from 1.0 to 37.1%. Moreover, in M. piperita, menthone, isomenthone and limonene were all increased in low stress and then were decreased in high stress conditions. In M. arvensis, the major compound; menthol was not affected but the content of menthone increased. It could be concluded that the salinity stress is detrimental but might be useful and may be recommended as an appropriate approach in improving the oil quality or to producing specific compounds under mild or moderate stress.

Effect of Row Ratio and Planting Methods on Growth, Water Use Efficiency, Yield and Economics of Wheat (Triticum aestivum L.) and Mentha (Mentha arvensis L.)

This study examines the intercropping system of wheat and mentha involves the simultaneous cultivation of two or more crops on the same piece of land for higher land productivity. Japanese mint, a member of the Lamiaceae family, has a potent essential oil. Oil extracted from the leaves for use in aromatherapy, food flavouring, and medicine. Hence, a field experiment was conducted to study the effect of Row ratio and Planting methods on Growth, Yield Performance of wheat (Triticum aestivum L.) and Mentha (Mentha arvensis L.) was conducted during 2016-17 at the Soil Conservation and Water Management Farm to investigate the response of mentha when intercropped with wheat under various row ratio and planting methods. The experiment was laid out in Randomized Block Design (RBD) with three replication keeping one variety of Mentha ‘Shivalik’& Wheat ‘Unnat Halna’. The experiment consisting of nine treatments are T1: Sole Mentha direct sowing (50cm apart), T2: Sole Mentha transplanting (50cm apart), T3: Sole Wheat (25cm apart), T4: Wheat+Mentha (d, 1:1), T5: Wheat+Mentha (t, 1:1), T6:Wheat+Mentha (d, 2:2), T7:Wheat+Mentha (t, 2:2), T8: Wheat Paired+Mentha (d, 2:3), T9: Wheat Paired+Mentha (t, 2:3). The results indicated that Sole Wheat (T3) had the highest plant population (662.40 initially, 326 final), plant height (83.80 cm), and grain yield (39.80 q/ha) for wheat. In the case of mentha, Wheat Paired+Mentha (T8) exhibited the maximum final plant population (119.43), plant height (72.51 at maturity), and equivalent oil yield (189.05 l/ha). Wheat Paired+Mentha (T8) also recorded the highest total water use (647 mm), water use efficiency (0.292 kg/ha/mm of water), land equivalent ratio (1.50), and economic parameters such as gross return (228,868), net return (160,828), and B:C ratio (3.36). The maximum oil yield (163.35 l/ha) was found in Sole Mentha direct sowing (T1).

Consensus in the use of ethnomedicinal plants during COVID-19 pandemic in and around Dhaka city

The present article deals with recording ethnomedicinal plants used by the people living in and around Dhaka city and also focuses on the plant species used during the pandemic to get relief from COVID-19. The information was gathered using open-ended and semi-structured questionnaires from 348 informants belonging to different classes of life. The survey has resulted in the recording of a total of 160 medicinal plant species belonging to 62 families and used for 157 ailments through 250 different formularies. Azadirachta indica A. Juss. was the most cited species in the study area. The highest factor informant consensus (FIC) was found in the Cuts and Wounds category, and Cynodon dactylon (L.) Pers. is the most cited species for this category. Acmella calva (DC.) R.K. Jansen was the culturally bound species attaining 100% Fidelity Level (FL) value. Among the ethnomedicinal plants, 40 species were found in the survey that were used by people to get relief from COVID-19. This interesting ethnomedicinal use is a new record for these 40 species. Most notable species are (Ocimum tenuiflorum L., Justicia adhatoda L., Centella asiatica (L.) Urban, Citrus aurantifolia (Christm. & Panzer) Swingle, Syzygium aromaticum (L.) Merr. & L.M. Perry, Mentha arvensis L., Zingiber officinale Rosc., Camellia sinensis (L.) O. Kuntze, and Nigella sativaL.Since ancient times, these species have been very popular and used to treat several ailments. The use of these species during the pandemic is a new hope to get relief from COVID-19 but this is very preliminary report. Further long-term study is needed to confirm the claim of these plants’ use against COVID-19. Due to excessive use during COVID-19, these species are very rare in the habitats in and around Dhaka city. Conservation measures should be applied to save these species from extinction. Bangladesh J. Plant Taxon. 30(1): 123-151, 2023 (June)

Role of Phenolic Acid Metabolism in Enhancing Bioactivity of Mentha Extract Fermented with Plant-Derived Lactobacillus plantarum SN13T

Plant-derived lactic acid bacteria are major fermentation organisms that can grow in medicinal herb extracts enriched with phytochemicals like glycosides, phenolic acids, flavonoids, and tannins. Fermentation with strain-specific Lactobacilli harboring metabolic enzymes can increase the bioactivity and bioavailability of medicinal herbs. Fermentation of extracts of Artemisia princeps and Paeonia lactiflora has been previously found to increase their bioactivities. Therefore, this study explores the possibility of increasing the bioactivity of Mentha arvensis (Mentha) extract against lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells by fermenting with plant-derived probiotic strains Lactobacillus (Lact.) plantarum SN13T and Pediococcus (Ped.) pentosaceus LP28. As a result, fermentation with SN13T significantly increased the bioactivity of Mentha extract as compared to unfermented or LP28-fermented extracts. This higher bioactivity was associated with the metabolism of rosmarinic acid (RA) and caffeic acid (CA), the major bioactive phenolic acids reported in Mentha, along with the production of the metabolite dihydrocaffeic acid (DHCA). DHCA was found to be a more potent LPS-induced nitric oxide (NO) inhibitor than its precursor phenolic acids. The metabolism of RA to DHCA via CA could be mediated by the enzymes cinnamoyl ester hydrolase and hydroxycinnamate reductases, encoded by the ceh gene and the hcrRABC gene operon, respectively, which were identified in the complete genome sequence of Lact. plantarum SN13T but were absent in Ped. pentosaceus LP28. The genes hcrA, hcrB, and hcrC were significantly and time-dependently overexpressed in Lact. plantarum SN13T when grown in the Mentha extract, suggesting the role of phenolic acid metabolism in enhancing its bioactivity.

Diversity of wild edible plants and fungi consumed by semi-nomadic Gaddi and Sippi tribes in Doda district of Union Territory of Jammu and Kashmir

Background: Consumption of Wild edible plants (WEPs) is a cultural heritage of tribal and other rural communities. WEPs have a vital role to play not only in ensuring nutritional security and combating malnutrition but also in meeting medicinal requirements, income generation for these communities, and crop improvement through plant breeding programmes. The present study was conducted with the objective of documenting traditional knowledge and practices of utilizing WEPs by Gaddi and Sippi tribes of Doda district of Union Territory of Jammu and Kashmir (JKUT). Methods: An ethnobotanical survey was conducted in five villages of Doda district (JKUT) inhabited by Gaddi and Sippi tribes. The random sampling method was used for the selection of informants. The data was collected through interviews and focused group discussion with the informants as per semi-structured schedules. The uses of WEPs were recorded as use-reports, and culture importance value (CI) and factor informant consensus (Fic) were calculated from these values. Results: A total of 77 species spread across 58 genera and 39 families were reported by 203 informants (148 males and 55 females). Angiosperms represented by 63 species were the major source of forest foods followed by 9 fungal species. WEPs with the highest number of species were vegetables (34 species), and fruits and nutraceuticals (27 species each). The most important species on the basis of CI were Geopora arenicola (Lev.) Kers. (CI = 0.66), Geopora sumneriana (Cooke) M. Torre. (0.60), Diplazium esculentum (Retz.) Sw. (0.56), Diplazium frondosum C. Chr. (0.54), and Mentha arvensis C. Presl. (0.52). Values of F­ic ranged between 0.91 (nutraceuticals) and 0.99 (cooked WEPs). The knowledge of WEPs significantly increased with age and decreased with education level of the informants. Conclusions: The Gaddi and Sippi tribes are utilizing a good number of WEPs, and more importantly they exchange this information among themselves. As far as future prospects of this study are concerned, nutritional and nutraceutical properties of species like Anethum sowa, Arisaema propinquum, Diplazium frondosum, Dipsacus inermis, Elaeagnus umbellate, Elwendia persica, Geopora sumneriana, Impatiens glandulifera, Morchella crassipes, Morchella conica, Prinsepia utilis, Sageretia thea, Solanum villosum, Thymus mongolicus, and Ziziphus oxyphylla may be studied to make them popular amongst masses. Keywords: Ethnobotany; Fungi; Gaddi and Sippi tribes; wild edible plants.

Antibacterial and Cytotoxic Activity of Mentha arvensis L. Leaves Methanolic Extract In vitro

Treatment with herbal drugs is considered one of the oldest and safest methods for treating various diseases. Mentha arvensis is a medicinal plant used worldwide in the pharmaceutical industry for its antibacterial, anticancer, antiallergic, and anti-inflammatory properties. In the present study, the antibacterial activity of the methanolic extract of M. arvensis leaves was tested at different concentrations (50, 100, 150, and 200 µg/ml) against bacteria isolated from human burn wound infections, including Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. The results demonstrated that at concentrations of 100, 150, and 200 µg/ml, the methanolic extract exhibited strong antibacterial activity against S. aureus and P. aeruginosa, while K. pneumoniae isolates were resistant to the extract at all tested concentrations. Further, the cytotoxic activity of the methanolic extract was assessed at various concentrations (100, 140, 160, 180, 220, and 250 µg/ml) against two cell lines: the RD tumor cell line and Vero normal cell line. The higher concentrations of the extract (220 and 250 µg/ml) showed potent cytotoxic effects on the RD cell line after 72 hours in a dose- and time-dependent manner. In contrast, the extract exhibited lower cytotoxic effects on Vero cells at the same concentrations after 72 h.

Antioxidant and Anti-Mutagenic Potential of Mint (Mentha Arvensis) and its Chemical Characterization by HPLC

Background: Mentha arvensis has been used for medicinal purposes by Asian countries for ages. It is used to cure various ailments such as diarrhea, asthma, piles, cancer, dementia, hyperglycemia, and aging. Aim: This study aims to evaluate the antioxidant and anti-mutagenic (geno-protective) properties of four different solvent extracts (methanol, ethanol, acetone, and distilled water). Methodology: The antioxidant properties were determined by DPPH and reducing power assays, while the DNA damaging assay was used to evaluate the anti-mutagenic activity. Results: Among the four extracts, the methanol extract of Mentha arvensis showed 30.48 ± 0.95 mg QE/gm of flavonoid, and the aqueous extract showed the highest total phenolic content (142.2±0.83 mg QE/gm) and total alkaloids content (78.2±0.21 mg TE/gm) compared to the other three solvent extracts. The ethanol extract of Mentha arvensis exhibited the maximum value for total tannin content (17.41±0.38 mg ETA/g CE) and reducing power (41.17±0.87 mg ETA/gm). It also exhibited significant geno-protective activity. Conclusion: These results indicate that among the four solvent extracts, the ethanolic extract of Mentha arvensis has the highest value for phenolic content. Moreover, all four solvents, except distilled water, showed valuable anti-mutagenic behavior. The HPLC results corroborate our findings, suggesting that these plants have great potential for use as antioxidant and anti-mutagenic agents. Keywords: Mentha arvensis, Antioxidant agents, Anti-mutagenic agents, Geno-protective activity.

Influence of titanium dioxide nanoparticles on growth, physiological attributes and essential oil production of Mentha arvensis L.

Influence of titanium dioxide nanoparticles on growth, physiological attributes and essential oil production of Mentha arvensis L.

Rhizomes Priming with Smoke–Water and Karrikinolide Enhance the Production of Essential Oil and Bioactive Constituents of Mentha arvensis L. via Improving the Growth and Physiological Attributes of Plant

Rhizomes Priming with Smoke–Water and Karrikinolide Enhance the Production of Essential Oil and Bioactive Constituents of Mentha arvensis L. via Improving the Growth and Physiological Attributes of Plant

Excessive copper induces toxicity in Mentha arvensis L. by disturbing growth, photosynthetic machinery, oxidative metabolism and essential oil constituents

Copper (Cu) is an essential micronutrient and plays numerous morphological, physiological, and biochemical functions in plant growth and development, however, the yield of crops is now threatened by the excessive accumulation of Cu in soil. In this study, the growth, photosynthetic traits, antioxidant activity, and oxidative biomarkers, as well as yield parameters including essential oil (EO) percentage and constituents of Mentha arvensis L., were investigated under different concentrations of Cu. The Cu treatment was given in the form of CuSO4 to the soil with various concentrations, viz. 10, 20, 40, 60, and 80 mg kg−1, and one set was kept as the control. Data reveal that the plants treated with higher Cu concentrations over 20 mg kg−1 showed toxicity symptoms i.e., stunted growth, inhibited photosynthetic pigments, accelerated antioxidant activity, evoked reactive oxygen species production, and decreased essential oil production. Notably, Cu applied at 20 mg kg−1 of soil significantly improved all the metrics examined, including the constituents of EO, especially menthol and menthyl acetate levels. Thus, the current study demonstrates that Cu supplementation at 20 mg kg−1 of soil proved advantageous, however, 80 mg kg−1 of soil-applied Cu resulted in a toxic response for the overall growth and development of M. arvensis plants.