The objective was to evaluate work fatigue and work organization in a food factory, in the state of Aragua, 2023. Research with a quantitative, field, descriptive, cross-sectional and correlational scope approach. The population was 135 workers and the sample was 101 workers, obtained by snowball sampling. The Yoshitake questionnaire (1978) modified by INSAT 1987 was applied. The statistical programs IBM SPSS ® Statistics Version 25 and EPIDAT were used. The sample was characterized using descriptive statistics and correlated by applying the chi-square and p-value tests. Results: The average age was 42.59 years, predominantly female; 44.6% single; 52.5% live with their partner.; 51.5% have secondary education; the family burden is less than or equal to three members in 61.4%; working workforce 80.2% all under rotating shift schemes; 63.4% have a length of service greater than 16 years; the travel time to the workplace is less than 30 minutes in 56.4%; 62.38% presented fatigue, in this group, 84.13% with general symptoms of fatigue, followed by 9.52% with mental fatigue, and 6.35% physical fatigue. It is concluded that 62.4% present mixed type work fatigue. Women, workers, those who live far away and those who worked shifts are more affected. There are no statistically significant differences between socio-occupational characteristics, shift work and the presence of fatigue.