Aim: To investigate the role dimensions, clinical competence, educational preparation and professional development needs of practice nurses. Methods: All practice nurses registered with professional development coordinators in Ireland (n=1519) were invited to participate by completing a postal questionnaire. This yielded a response rate of 30% (n=451). Descriptive statistical analysis of the data was conducted using SPSS. Results: Regular activities included immunization, phlebotomy, management of laboratory results, wound care and electrocardiography. Cervical screening, dietary advice and health screening were also indicated by over 85% of practice nurses as a regular role dimension, and the majority of practice nurses perceived their level of competency in these areas as proficient or expert. Aspects of chronic disease management, namely in the area of diabetes and hypertension, were cited by over 78% of practice nurses as a regular role. Less than 60% of practice nurses deemed themselves expert or proficient in this area. Education and training deficits were noted in chronic disease management, nurse prescribing, mental health advice and women's health. A lack of resources to implement nurse-led clinics was evident; however, the majority of practice nurses had support from their employing GP to achieve professional development goals. Conclusions: It is evident that practice nurses are involved at varying levels across a variety of clinical activities. Results indicated varying levels of competence and were positively affected by further education and the frequency at which the role was performed. Policies need to be developed concerning the role and future of practice nursing in primary care.