Memory Profile Research Articles

Latent structure of working memory and emotion regulation in pediatric ADHD

ABSTRACT Working memory is a key cognitive function that is often implicated in ADHD and may represent an underlying cognitive endophenotype of the disorder. Working memory is related to emotion regulation, which is a common area of impairment in children with ADHD. Given the high rate of comorbid diagnoses with ADHD, children with comorbid internalizing, externalizing, and developmental disorders may present with variable working memory profiles and subsequent emotion regulation deficits. This study aimed to adequately characterize the latent structure of working memory impairments and emotional regulation outcomes in a clinical sample of children ages 6–16 with ADHD. This study also examined the interplay between the identified working memory/emotion regulation patterns, demographic characteristics, and the role of comorbid diagnoses. Results highlighted two distinct, invariant, unrestricted classes of working memory/emotion regulation. Class 1 (Average; 62% of the sample) had significantly lower digit-span scores, and generally persevered emotion regulation functioning per parent/teacher report. Class 2 (Emotionally Dysregulated) had average working memory scores, and elevated emotion regulation problems. The working memory indicators had small correlations with parents (and not teacher) measures of emotion regulation. Finally, latent class membership did not differ by comorbid diagnosis, age, gender, or verbal IQ. Findings elucidate heterogeneity in common domains affected by ADHD and suggest that this heterogeneity may not be due to demographic/comorbidity factors. The role of varying information reports is discussed, and potential assessment and treatment implications are highlighted.

A-216 Construct Validity of The National Institutes of Health Toolbox- Cognition Battery (NIHTB-CB) in Children and Adolescents with Cerebral Cavernous Malformation, Type 1 (CCM-1) and their Non-Affected Relatives

Abstract Objective: This study assessed the convergent validity of the NIHTB-CB in a sample of children and adolescents with CCM-1 and non-affected relatives. Method: Twenty-two participants with CCM-1 and 7 non-affected relatives completed the NIHTB-CB and traditional neuropsychological measures. The following domains were assessed: memory (NIHTB-CB Picture Sequencing Memory and Child and Adolescent Memory Profile- Screening Index), word reading (NIHTB-CB Oral Reading and Wide Range Achievement Test-4th Word Reading [WRAT-4 WR]), processing speed (NIHTB-CB Pattern Comparison and Symbol Digit Modalities Test), and attention/working memory (NIHTB-CB List Sorting and Digit Span). Results: The non-affected group scored higher than the CCM-1 group on WRAT-4 WR (t = 2.68, p = .02) and NIHTB-CB Oral Reading (t = 2.18, p = .05). The groups did not differ on the other measures (p > .05). Pearson’s correlations ranged from .45 for memory to .81 for word reading, demonstrating adequate construct validity for memory, processing speed, and attention/ working memory and good to very good for word reading. The NIHTB-CB was more likely to identify participants as impaired for memory (17.2% vs 6.9%) and processing speed (62.1% vs. 3.4%). The traditional attention/working memory measure was more likely to identify participants as impaired (27.6% vs 3.4%). Impairment rates were similar for the word reading measures. Conclusions: Of the domains considered, convergent validity was best established for word reading. Although correlations were adequate, rates of impairment differed for memory, processing speed, and attention/ working memory, suggesting that caution is warranted when comparing the NIHTB-CB to traditional measures in these areas.

A-252 A Suspected Case Of Limbic-Predominant Age-Related TDP-43 Encephalopathy (LATE) in an Older Adult

Abstract Objective: LATE is a common but underrecognized neuropathology that causes a progressive amnestic syndrome in older adults which mimics an Alzheimer’s disease (AD) clinical profile. Method: A 75-year-old female with a history of progressive cognitive impairment presented to a multidisciplinary neurology clinic. Neurocognitive screening during her neurological exam showed an amnestic memory profile. A work-up for medical causes for her cognitive impairment was negative. She had prominent hippocampal atrophy on MRI with a negative amyloid PET scan, raising concerns for LATE. She was referred for a comprehensive neuropsychological evaluation. Results: Neuropsychological testing confirmed an amnestic mild dementia profile in the context of her other cognitive skills (e.g., attention, executive functioning, processing speed, word finding, and visuospatial abilities) being largely preserved. On self-report questionnaires, the patient denied having any significant symptoms of depression or anxiety. Conclusions: LATE is described as causing a primarily amnestic neurocognitive profile with associated hippocampal atrophy and this was well demonstrated in our patient. While the diagnosis of LATE cannot be definitively made until autopsy, the absence of amyloid on her PET scan highly suggests a non-AD pathology like LATE. A limitation of the evaluation is that tau biomarker tests were not performed, making primary age-related tauopathy (PART) another possible pathology or co-pathology. Overall, this case study illustrates the importance of multidisciplinary assessment to help facilitate differential diagnosis for neurodegenerative conditions associated with amnestic cognitive impairment, especially when a non-AD pathology is suspected.

Learning Strategies and Verbal Memory Deficits on the Shiraz Verbal Learning Test in Patients with Alzheimer’s Disease and Amnestic Mild Cognitive Impairment

Background: Much remains unknown regarding the nature of the memory profile of individuals with amnestic mild cognitive impairment (aMCI). One of the questions is whether they first encounter an encoding/storage defect or retrieval memory profile difficulties. Objectives: The present study aimed to shed light on this by evaluating learning strategies and memory process deficits in individuals with aMCI and Alzheimer’s disease (AD). Methods: The Shiraz Verbal Learning Test (SVLT) was used to assess and compare verbal memory performance and learning strategies among three groups of individuals, including patients with aMCI, AD cases, and healthy individuals. The study groups were compared using most indices of the SVLT. Results: A pattern of memory impairment was found in the aMCI group, and the indicators included defects in immediate learning, a poor learning slope, rapid forgetting, and a poor function in delayed recall. This was similar to the representations of the individuals with AD. However, the aMCI group acted differently from the AD and healthy groups when it came to learning strategies. Specifically, they mostly used serial clustering. Furthermore, the results of serial position effects showed no significant difference between the three groups in terms of primary/recency effects. Conclusions: The findings of the present research suggested that individuals with aMCI, similar to patients with AD, initially develop defects in encoding and storage (to a lesser degree), followed by retrieval memory problems. Our results also supported that SVLT can be a reliable diagnostic tool to estimate aMCI progression or the prodromal stage of AD type dementia.

Abstract 2048: In vitro and in vivo characterization of non-oncolytic engineered arenavirus vectors for cancer immunotherapy

Abstract Cytotoxic CD8+ T cells are paramount for effective cancer immunotherapy. We engineered two distantly related arenaviruses, lymphocytic choriomeningitis virus (LCMV) and Pichinde virus (PICV), encoding the same non-oncogenic HPV16 E7E6 fusion protein to effectively mount and maintain tumor specific CD8+ T cell responses. The designated vectors, HB-201 (LCMV) and HB-202 (PICV), are currently in an ongoing Phase I/II open labelled clinical trial of HB-201 single vector therapy and HB-202/HB-201 alternating two-vector therapy in patients with treatment-refractory HPV16+ cancers (NCT04180215). To characterize the immunogenic properties of both vectors, we designed a comprehensive set of preclinical and translational experiments utilizing human PBMCs, artificial APCs and HPV16 E6 and E7 specific reporter T cells. This was done in conjunction with a preclinical model for HPV16 associated cancer (TC-1). HB-201 (LCMV) and HB-202 (PICV) surrogate vectors encoding GFP readily infected human monocytes, plasmacytoid dendritic, and conventional dendritic cells. In addition, HB-201 and HB-202 vectors successfully induced antigen presentation of E7 and E6 epitopes as evidenced by TCR reporter cells. Innate immune cell activation and cytokine responses to HB-202 were slightly lower compared to HB-201, findings which are consistent with different memory profiles of E7 specific CD8+ T cells in non-tumor bearing mice. In a therapeutic setting of the TC-1 tumor model, both vectors were comparably effective, showing major infiltration of CD8+ T cells into the tumor microenvironment, tumor growth delay and a significantly prolonged survival already after a single administration. In this data set we have confirmed the strong immunogenicity of the engineered arenavirus platform leading to efficient tumor control in a relevant mouse model for HPV16+ cancers, which further supports the clinical development of our novel arenavirus platform. Citation Format: Josipa Raguz, Theresa Kleissner, Sandra Rosskopf, Mohamed Habbeddine, Katharina Lechner, Valentin Just, Donna Edwards, Igor Matushansky, Christoph Lampert, Klaus K. Orlinger, Henning Lauterbach. In vitro and in vivo characterization of non-oncolytic engineered arenavirus vectors for cancer immunotherapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2048.

Consistency Hierarchy of Reservoir Computers.

We study the propagation and distribution of information-carrying signals injected in dynamical systems serving as reservoir computers. Through different combinations of repeated input signals, a multivariate correlation analysis reveals measures known as the consistency spectrum and consistency capacity. These are high-dimensional portraits of the nonlinear functional dependence between input and reservoir state. For multiple inputs, a hierarchy of capacities characterizes the interference of signals from each source. For an individual input, the time-resolved capacities form a profile of the reservoir's nonlinear fading memory. We illustrate this methodology for a range of echo state networks.

Dietary Intake of Polyphenols Enhances Executive/Attentional Functioning and Memory with an Improvement of the Milk Lipid Profile of Postpartum Women from Argentina.

Puerperium may lead to memory and executive/attentional complaints that interfere with women’s daily life. This might be prevented by dietary compounds, such as neuroprotective polyphenols. Their bioactivity depends on their effects on lipid metabolism in different tissues, such as the brain, fat, and breast. Thus, a polyphenol-related cognitive improvement may be associated with changes of lipids in human milk, which are key for infant neurodevelopment. A cross-sectional study was conducted on 75 postpartum women from Córdoba (Argentina), involving several neuropsychological tests. Diet was registered to identify polyphenol intake and food pattern adherence, with sociodemographic and other psychological variables (insomnia, stress, subjective cognitive complaints) being also studied. Triacylglycerols, cholesterol, and their oxidative forms were analyzed as milk biomarkers. Multivariate statistical methods were applied. Results confirmed that women who consumed polyphenols presented better executive/attentional performance (i.e., higher correct responses, conceptual level responses, complete categories, verbal fluency; lower attentional interferences, and perseverative errors) and word retention with lower interference. Polyphenols were positively associated with milk lipids, which were higher in women with better cognition. Furthermore, they had lower oxidized triacylglycerols. In conclusion, polyphenolic intake during postpartum may improve executive/attentional functioning, memory, and milk lipid profile.

Information management for trust computation on resource-constrained IoT devices

Resource-constrained Internet of Things (IoT) devices are executing increasingly sophisticated applications that may require computational or memory intensive tasks to be executed. Due to their resource constraints, IoT devices may be unable to compute these tasks and will offload them to more powerful resource-rich edge nodes. However, as edge nodes may not necessarily behave as expected, an IoT device needs to be able to select which edge node should execute its tasks. This selection problem can be addressed by using a measure of behavioural trust of the edge nodes delivering a correct response, based on historical information about past interactions with edge nodes that are stored in memory. However, due to their constrained memory capacity, IoT devices will only be able to store a limited amount of trust information, thereby requiring an eviction strategy when its memory is full of which there has been limited investigation in the literature. To address this, we develop the concept of the memory profile of an agent and that profile’s utility. We formalise the profile eviction problem in a unified profile memory model and show it is NP-complete. To circumvent the inherent complexity, we study the performance of eviction algorithms in a partitioned profile memory model using our utility metric. Our results show that localised eviction strategies which only consider one specific type of information do not perform well. Thus we propose a novel eviction strategy that globally considers all types of trust information stored and we show that it outperforms local eviction strategies for the majority of memory sizes and agent behaviours. In this paper, we develop a concept of information utility to a trust model and formalise the problem of information eviction, which we prove to be NP-complete. We then investigate the usefulness of different eviction strategies to maximise the utility of information stored to enable trust-based task offloading.

Political Socialization in a Changing Society: A Crisis of Value Orientations or Asynchronization of National Memories?

The article examines qualitative changes in the socio-cultural parameters of intergenerational communication that impact social cohesion and civic identification in contemporary society. Diversification of symbol production and an increase in the number of agents of political communications, a greater heterogeneity of political ideologies, and modes of political representation shape political processes. To adequately address these changes, one needs new theoretical models of political socialization. Such models would examine youth political incorporation as a particular form of communication predicated on the spatial and temporal design of political events within national communities. The focus on generational differences in the interpretation of political events helps explain youth positioning vis-à-vis older generations. In this case, political socialization goes beyond the processes of the younger generation’s adaptation to institutions and value-normative regimes of the “adult” society. It is a communicative process of establishing generational political expectations. Analysis of the legitimating profiles of national memory—those that include competing symbolic representations of images of the future and the past, typologies of the heroic, concepts of guilt and responsibility—is crucial to studying the political socialization of the younger generation. The authors emphasize the significance of both a theoretical and applied analysis of symbolic structures of political memory and the role of iconic power in understanding intergenerational continuity and change. Drawing on the methods of cultural sociology, the authors outline novel theoretical approaches to studying youth political socialization in today’s society.

A random walk model with a mixed memory profile: Exponential and rectangular profile

The theory of Markovian random walks is consolidated and very well understood, however the theory of non-Markovian random walks presents many challenges due to its remarkably rich phenomenology. An important open problem in this context is to study how the diffusive properties of random walk processes change when memory-induced correlations are introduced. In this work we propose a model of a random walk that evolves in time according to past memories selected from rectangular (flat) and exponentially decaying memory profiles. In this mixed memory profile model, the walker remembers either the last B steps with equal a priori probability or the steps A prior to B with exponentially decaying probability, for a total number of steps equal to A+B. The diffusive behavior of the walk is numerically examined through the Hurst exponent (H). Even in the lack of exact solutions, we are still able to show that the model can be mapped onto a RW model with rectangular memory profile.

Memory forensic analysis of a programmable logic controller in industrial control systems

In industrial control systems (ICS), programmable logic controllers (PLCs) are used to automate physical processes such as nuclear plants and power grid stations, and are often subject to cyber attacks. As in conventional IT domain, the memory analysis of the PLCs can help answer important forensic questions about the attack, such as the presence of malicious firmware, injection of modified control logic (the program running on the PLC), and manipulation of I/O devices (e.g., sensors and actuators). Unlike conventional IT domain, PLCs have heterogeneous hardware architecture, proprietary firmware and control software, making it challenging to employ a unified framework for their memory forensics. For merely extracting artifacts of forensic importance, reverse-engineering the firmware is a tedious task, and the effort needs to be repeated for every PLC model. As a community, a step-wise approach to tackle this challenge is to analyze the memory of specific PLCs, and subsequently find a generic framework applicable to all PLCs. Our work is a step forward in this direction. By following a methodology that focuses on the functional layer of PLCs instead of reverse engineering the firmware, we analyze the digital forensic artifacts available in a common PLC, Allen-Bradley ControlLogix 1756-L61. Before diving into the memory dump, we analyze the PLC control software to create a list of important artifacts that are sure to exist in the PLC memory dump. The approach employs a setup where PLC control software RSLogix-5000 is connected to the PLC, and the memory dump can be obtained as and when needed. We create test cases that sequentially highlight each category of artifacts, followed by an examination of the resultant impact on memory. After attaining the listed artifacts, we employ conventional string and known data searches to extract interesting information present in this PLC's memory. The memory analysis profile, presented as a Python library and shared with the community, can help a forensic investigator to readily extract forensic artifacts from the same model's controller. The adopted approach may help researchers in creating memory profile of other PLCs, and ultimately formulating a generic PLC memory analysis framework.

An Investigation of Working Memory Profile and Fluid Intelligence in Children With Neurodevelopmental Difficulties.

The present study aims to evaluate the distinct patterns of working memory (WM) capacity of children with Developmental Language Disorder (DLD), High-functioning children with Autism Spectrum Disorder (ASD) and children with Down syndrome (DS). More specifically, the current study investigates the complex relationship of fluid intelligence and WM between 39 children with DLD, 20 H igh-functioning children with ASD, and 15 children with DS. All children were evaluated in different measures of Phonological Working Memory, Visual-spatial Working Memory whereas Fluid Intelligence was measured with Raven Progressive Matrices. The result analysis revealed a significant difference among the three groups, both among each function separately and the correlations among them, as well. The results revealed that the DLD groups and High-functioning ASD group exhibited a common picture or an overlap of performances in all Phonological and Visuo-spatial working memory measures, except Backward Digit Recall task. As for the DS group research findings revealed different and unique working memory patterns in comparison to DLD group and High-functioning ASD. Their differences have been studied and further conclusions have been drawn about the different patterns of working memory among the three clinical groups. The implications of these findings are discussed in light of support for learning. The common profile that characterize the two developmental conditions and the distinct pattern of working memory performance in DS group underlies the need for further research in the field.

Characterization of memory profile in idiopathic REM sleep behavior disorder

ABSTRACT Objective The present study aims to examine whether declarative memory dysfunction relates to impaired core memory mechanisms or attentional and executive dysfunction in idiopathic REM Sleep Behavior Disorder (iRBD). Method In this observational, cross-sectional study, were enrolled 82 individuals with the diagnosis of iRBD according to the International Classification of Sleep Disorders and 49-matched healthy controls fulfilling inclusion criteria. All participants underwent two memory tasks, namely the Rey Auditory Verbal Learning Test (RAVLT) and Memory Binding Test (MBT), which include conditions of varying degrees of dependence on executive functioning, as well as different indicators of core memory processes (e.g., learning, retention, relational binding). Results We used Bayesian multivariate generalized linear model analysis to evaluate the effect of iRBD on memory performance controlled for effects of age and sex. Individuals with iRBD displayed worse memory performance in the delayed free recall task (b = −0.37, 95% PPI [−0.69, −0.05]), but not on delayed recognition of the same material. Their performance in cued recall tasks both in immediate and delayed conditions was in comparison to controls relatively spared. Moreover, the deficit in delayed free recall was mediated by attention/processing speed. Conclusions In iRBD, we replicated findings of reduced free recall based on inefficient retrieval (retrieval deficit), which was small in terms of effect size. Importantly, the memory profile across measures does not support the presence of core memory dysfunction, such as poor learning, retention or associative binding.

TmPSMA-02: A CD2 endodomain containing double armored PSMA CAR T with enhanced efficacy and lower immune toxicity.

158 Background: Early clinical responses to anti-PSMA Chimeric Antigen Receptor T cells (CARs) have been observed but much like the leukemia experience, significant toxicity from macrophage activation (MAS) has also been observed Building on our experience with a first in-human armoured CAR (TmPSMA-01) targeting PSMA (with a 41BB and CD3 endodomain) coupled with a dominant-negative TGFBR2 module, where we observed anti-tumor effects but also immune-mediated toxicity, we designed a CAR with additional armour, a PD1-CD28 switch, to overcome the immune suppressive microenvironment and further improve efficacy. We successfully built tricistronic constructs that expresses the CAR, a dnTGFBR2 module and a switch receptor for PD1 and CD28. We compared the 41BB endodomain and the CD2 domain, which has shown preliminary effects reducing cytokine release. We hypothesized that these changes would increase efficacy in immune-suppressive solid tumors while reducing excessive cytokine release and untoward immune toxicity. Methods: CAR constructs were synthesized into a self-inactivating lentiviral backbone and CAR T cells manufactured comprising a control TmPSMA-01 construct and two primary constructs: PSMA-CD2z:dnTGFBR:PD1-CD28 and PSMA-41BBz:dnTGFBR:PD1-CD28. Expression of the CAR as well as T cell markers were determined by flow cytometry, and CAR affinity for target by Lumicks. CAR activation and cytotoxicity were assessed by ELISPOT, xCelligence and Celligo standard assays. A co-culture macrophage activation system was applied to each construct to assess the potential of each construct to induce MAS-like responses. Results: A significant difference was seen in the MFI of the CAR, with the CD2 based CAR consistently ̃50% lower than the 41BB and this correlated to a faster off-rate from target cells measured by Lumicks. There was no difference in exhaustion or memory markers, and cytotoxicity across a range of E:T ratios was equivalent between the CD2 and 41BB constructs. Preliminary data indicated the CD2 endodomain preserved T cell activation but with lower cytokine release. The CD2 constructs consistently produced significantly less total IL2 and IFNg after exposure to targets, yet had similar numbers of ELISPOT positive cells for IL2 and IFNg indicating less cytokine production per kill. This also resulted in significantly less macrophage based cytokines such as IL6 and IL8 in the coculture model. Using a cell line knocked out for PDL1/2 and then reengineered for constitutive PDL1/2 expression, we demonstrated improved speed of killing in the xCelligence system dependent on the PD1:CD28 switch but no increase in cytokine release. Conclusions: A CD2 based endodomain tricistronic CAR targeting PSMA (TmPSMA-02) maintained cytotoxicity, memory profile and activation while having a significantly reduced risk of inducing macrophage activation.

Remembering Molly: Immediate and delayed false memory formation after acute MDMA exposure

The entactogen 3,4-Methylenedioxymethamphetamine (MDMA) is increasingly being recognized for its therapeutic potential but is also widespread in nightlife settings where it may co-occur with crime. Since previous research detected impaired verbal memory during acute MDMA intoxication, understanding the drug's ramifications in an applied legal context becomes crucial. We conducted a double-blind, placebo-controlled trial to examine acute and delayed effects of MDMA (75 mg) on false memory in 60 healthy volunteers with a history of MDMA use, using three well-established false memory methods: a basic, associative word list (Deese/Roediger-McDermott (DRM)) paradigm and two applied misinformation tasks using a virtual reality crime. Memory was tested immediately (encoding and retrieval under drug influence) and 1 week later (retrieval when sober). Small MDMA-induced impairments of true memory in the word list task were detected at both time points. MDMA increased false memory for related but non-critical lures during the immediate test, and decreased false memory for critical lures after a delay. Episodic memory assessed in the misinformation tasks was not consistently affected. Findings indicate a complex memory profile but no heightened vulnerability to external suggestion in response to MDMA intoxication. Recommendations for future applied legal psychological research include adding measures of recall on top of recognition, using study designs that separate the different memory phases, and potentially testing higher doses. Further research on false memories and suggestibility using imagination procedures can also be relevant for the clinical context.

Effects of BCG vaccination on donor unrestricted T cells in two prospective cohort studies.

SummaryBackgroundNon-protein antigen classes can be presented to T cells by near-monomorphic antigen-presenting molecules such as CD1, MR1, and butyrophilin 3A1. Such T cells, referred to as donor unrestricted T (DURT) cells, typically express stereotypic T cell receptors. The near-unrestricted nature of DURT cell antigen recognition is of particular interest for vaccine development, and we sought to define the roles of DURT cells, including MR1-restricted MAIT cells, CD1b-restricted glucose monomycolate (GMM)-specific T cells, CD1d-restricted NKT cells, and γδ T cells, in vaccination against Mycobacterium tuberculosis.MethodsWe compared and characterized DURT cells following primary bacille Calmette-Guerin (BCG) vaccination in a cohort of vaccinated and unvaccinated infants, as well as before and after BCG-revaccination in adults.FindingsBCG (re)vaccination did not modulate peripheral blood frequencies, T cell activation or memory profiles of MAIT cells, CD1b-restricted GMM-specific and germline-encoded mycolyl-reactive (GEM) cells or CD1d-restricted NKT cells. By contrast, primary BCG vaccination was associated with increased frequencies of γδ T cells as well as a novel subset of CD26+CD161+TRAV1-2− IFN-γ-expressing CD4+ T cells in infants.InterpretationOur findings, that most DURT cell populations were not modulated by BCG, do not preclude a role of BCG in modulating other qualitative aspects of DURT cells. More studies are required to understand the full potential of DURT cells in new TB vaccine strategies.FundingAeras, the National Institutes of Health, and the Bill and Melinda Gates Foundation.

Political identity and national memory: The conflict of contemporary political narratives

The article examines the place and the role of political narratives of national memory in the processes of political identification of modern political communities. The authors prioritize contemporary socio-cultural research strategies of political phenomena, which interpret political identification processes in the methodological context of the dynamics of national memory’s symbolic structures. The proliferation of such communicative practices as storytelling, political performance, post-factual politics and political imaginary complicates the problem of political identification and its narrative design in modern society and gives rise to multiple nationalist, populist, and ethno-political discourses. In this context, the significance of the theoretical understanding of national codes of communal political identification and an ability to forecast the symbolic contours of a new, more stable narrative of national identity based on codes of solidarity and civic patriotism increases. The study of the impact of symbolic configurations of legitimating profiles of national memory on specifics of everyday political narratives and the discourses of civic patriotism as the semantic core of national identity is particularly important in the study of contemporary political identification. The description of the profiles of the national memory legitimation necessitates a study of the conflict dynamics of the symbolic contours of the national memory, which includes competing semantic components (images of the past, political characteristics of elites, typology of the heroic, priority strategies and practices of fighting “enemies”). In turn, these processes determine the nature of the emergence and multiplication of identity conflicts. Using methodological premises of the cultural and sociological analysis of the dynamics of modern political cultures as symbolic forms of national memory, the authors propose new theoretical approaches to the study of the political identification processes and conflicts among basic political narratives in the realities of contemporary political communicative practices.

Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac).

Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

Exploit-oriented Automated Information Leakage

PDF HTML XML Export Cite reminder Exploit-oriented Automated Information Leakage DOI: 10.21655/ijsi.1673-7288.00290 Author: Affiliation: Clc Number: Fund Project: Article | Figures | Metrics | Reference | Related | Cited by | Materials | Comments Abstract:Automatic Exploit Generation (AEG) has become one of the most important ways to demonstrate the exploitability of vulnerabilities. However, state-of-the-art AEG solutions in general assume the target system has no mitigations deployed, which is not true in modern operating systems since they often deploy mitigations like Data Execution Prevention (DEP) and Address Space Layout Randomization (ASLR). This paper presents the automatic solution EoLeak that can exploit heap vulnerabilities to leak sensitive data and bypass ASLR and DEP at the same time. At a high level, EoLeak analyzes the program execution trace of the Proof-Of-Concept (POC) input that triggers the heap vulnerabilities, characterizes the memory profile from the trace, locates sensitive data (e.g., code pointers), constructs leakage primitives that disclose sensitive data, and generates exploits for the entire process when possible. We have implemented a prototype of EoLeak and evaluated it on a set of Capture The Flag (CTF) binary programs and several real-world applications. Evaluation results reveal that EoLeak is effective at leaking data and generating exploits. Reference Related Cited by

Dual IGF1R/IR inhibitors in combination with GD2-CAR T-cells display a potent anti-tumor activity in diffuse midline glioma H3K27M-mutant.

BackgroundDiffuse midline gliomas (DMG) H3K27M-mutant, including diffuse intrinsic pontine glioma (DIPG), are pediatric brain tumors associated with grim prognosis. Although GD2-CAR T-cells demonstrated significant anti-tumor activity against DMG H3K27M-mutant in vivo, a multimodal approach may be needed to more effectively treat patients. We investigated GD2 expression in DMG/DIPG and other pediatric high-grade gliomas (pHGG) and sought to identify chemical compounds that would enhance GD2-CAR T-cell anti-tumor efficacy.MethodsImmunohistochemistry in tumor tissue samples and immunofluorescence in primary patient-derived cell lines were performed to study GD2 expression. We developed a high-throughput cell-based assay to screen 42 kinase inhibitors in combination with GD2-CAR T-cells. Cell viability, western blots, flow-cytometry, real time PCR experiments, DIPG 3D culture models, and orthotopic xenograft model were applied to investigate the effect of selected compounds on DIPG cell death and CAR T-cell function.ResultsGD2 was heterogeneously, but widely, expressed in the tissue tested, while its expression was homogeneous and restricted to DMG/DIPG H3K27M-mutant cell lines. We identified dual IGF1R/IR antagonists, BMS-754807 and linsitinib, able to inhibit tumor cell viability at concentrations that do not affect CAR T-cells. Linsitinib, but not BMS-754807, decreases activation/exhaustion of GD2-CAR T-cells and increases their central memory profile. The enhanced anti-tumor activity of linsitinib/GD2-CAR T-cell combination was confirmed in DIPG models in vitro, ex vivo, and in vivo.ConclusionOur study supports the development of IGF1R/IR inhibitors to be used in combination with GD2-CAR T-cells for treating patients affected by DMG/DIPG and, potentially, by pHGG.