Gel Melting Research Articles

Spectrophotometric investigation of gel formation in water solution of agar

The temperature and concentration dependences of the transmission spectrum of an aqueous solution of agar are investigated. Measurements taken in the heating and cooling modes show strong thermal hysteresis in the spectral properties. Analysis of the results suggests that the formation of a gel network occurs in a much narrower temperature range than its collapse. The study of the temperature dependence of transmittance indicates that the association bispirals by cooling the solution and responsible for the formation of network occurs at temperature about [Formula: see text]C. A breakdown of these associates by heating of the gel begins at [Formula: see text]C and covers a wide temperature range. The gel point [Formula: see text] and the gel melting point [Formula: see text] increase with increasing polymer concentration, but for [Formula: see text] this dependence is stronger. Growth of turbidity (light scattering) of gel by increasing of agar concentration is due to the increasing of number and size of gel associates.

Studies on the physicochemical properties, gelling behavior and drug release performance of agar/κ-carrageenan mixed hydrogels

A series of agar/κ-carrageenan mixed hydrogels with different mass ratios were prepared, and their physicochemical properties, gelling behavior and drug release performance were determined and analyzed. The results showed that the gel strength, the gelling temperature and the gel melting temperature decreased with the increase of κ-carrageenan, while the apparent viscosities increased. Optical rotation and differential scanning calorimetry (DSC) indicated that there did exist intermolecular interactions between agar and κ-carrageenan, and the detailed gelling mechanism of the mixed hydrogels was proposed, which was different from that of the previous research. Besides, agar/κ-carrageenan mixed hydrogels were used as carriers for the delivery of metformin hydrochloride (MET). The results showed that the drug loading efficiency and the sustained release capacity of agar hydrogels could be enhanced by the addition of κ-carrageenan, and the release profile was mainly dominated by the electrostatic interaction between the MET and the polysaccharides. These results indicated that κ-carrageenan had the potential to improve the physicochemical properties and drug release performance of agar hydrogel.

Poly(ε-Caprolactone)/Poly(Lactic Acid) Blends Compatibilized by Peroxide Initiators: Comparison of Two Strategies.

Poly(ε-caprolactone) (PCL) and poly(lactic acid) (PLA) blends were compatibilized by reactive blending and by copolymers formed during reaction in the solution. The reactive blending of PCL/PLA was performed using di-(2-tert-butyl-peroxyisopropyl)benzene (BIB) or dicumyl peroxide (DCP) as radical initiator. PCL-g-PLA copolymers were prepared using 1.0 wt. % of DCP or BIB via reaction in solution, which was investigated through a Fourier transform infrared spectrometry (FTIR) and nuclear magnetic resonance (NMR) in order to better understand the occurring mechanisms. The effect of different additions such as PCL-g-PLA copolymers, DCP, or BIB on the properties of PCL/PLA blends was studied. The unmodified PCL/PLA blends showed a sea-island morphology typical of incompatible blends, where PLA droplets were dispersed in the PCL matrix. Application of organic peroxides improved miscibility between PCL and PLA phases. A similar effect was observed for PCL/PLA blend compatibilized by PCL-g-PLA copolymer, where BIB was used as initiator. However, in case of application of the peroxides, the PCL/PLA blends were cross-linked, and it has been confirmed by the gel fraction and melt flow index measurements. The thermal and mechanical properties of the blends were also investigated by means of differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA), and tensile strength.

UV-Triggered On-Demand Temperature-Responsive Reversible and Irreversible Gelation of Cellulose Nanocrystals.

We show ionically cross-linked, temperature-responsive reversible or irreversible hydrogels of anionic cellulose nanocrystals (CNCs) and methacrylate terpolymers by mixing them homogeneously in the initially charge-neutral state of the polymer, which was subsequently switched to be cationic by cleaving side groups by UV irradiation. The polymer is a random terpolymer poly(di(ethylene glycol) methyl ether methacrylate)-rnd-poly(oligo(ethylene glycol) methyl ether methacrylate)-rnd-poly(2-((2-nitrobenzyl)oxycarbonyl)aminoethyl methacrylate), that is, PDEGMA-rnd-POEGMA-rnd-PNBOCAEMA. The PDEGMA and POEGMA repeating units lead to a lower critical solution temperature (LCST) behavior. Initially, homogeneous aqueous mixtures are obtained with CNCs, and no gelation is observed even upon heating to 60 °C. However, upon UV irradiation, the NBOCAEMAs are transformed to cationic 2-aminoethyl methacrylate (AEMA) groups, as 2-nitrobenzaldehyde moieties are cleaved. The resulting mixtures of anionic CNC and cationic PDEGMA-rnd-POEGMA-rnd-PAEMA show gelation for sufficiently high polymer fractions upon heating to 60 °C due to the interplay of ionic interactions and LCST. For short heating times, the gelation is thermoreversible, whereas for long enough heating times, irreversible gels can be obtained, indicating importance of kinetic aspects. The ionic nature of the cross-linking is directly shown by adding NaCl, which leads to gel melting. In conclusion, the optical triggering of the polymer ionic interactions in combination with its LCST phase behavior allows a new way for ionic nanocellulose hydrogel assemblies.

Structure evolution of the interface between graphene oxide-reinforced calcium silicate hydrate gel particles exposed to high temperature

Structure, reactivity, and dynamics of the interface between graphene oxide (GO)-reinforced calcium silicate hydrate (CSH) gel particles at the temperature from 900 to 1100 K are investigated by means of reactive molecular dynamics. The results show that, high temperature weakens the CSH structure, including the stretching of Si-O bonds, the decomposition of silicate chains, and the hydrolysis weakening caused by water molecules. The high kinetic energy and weakened structure result in the melting and diffusion of CSH gel. However, the incorporation of GO can effectively stabilize the CSH structure by following three factors. (1) GO prevents the diffusion of the gel, and the packing of gel on the GO nanosheet increases the compatibility between them. (2) Reduction reaction of GO creates the defects on the carbon plane, providing the space for silicate chains crossing GO sheet. (3) Frequency exchange of oxygen between GO and CSH promotes the formation of chemical bonding between them. The improvement of the stability of CSH gel can effectively reduce the ablation velocity under high temperature.

Effects of γ-polyglutamic acid on the gelling properties and non-covalent interactions of fish gelatin.

The effects of γ-polyglutamic aid (γ-PGA) on the gelling properties and non-covalent interactions of fish gelatin were investigated. The gel strength and melting temperature of fish gelatin gradually increased, with increasing γ-PGA concentration, although there was no significant change when the γ-PGA concentration was greater than 0.04%. As the concentration of γ-PGA increased, the electrostatic interaction of fish gelatin increased and the hydrophobic interaction between gelatin molecules decreased. The fish gelatin system was comprised of γ-PGA concentrations of 0.04 and 0.06% showing a strong hydrogen bond. When the γ-PGA concentration increased from 0 to 0.04%, more phenolic hydroxyl groups in the tyrosine residue tended to form hydrogen bonds with the protein. However, an additional increase in γ-PGA concentration to 0.1% led to enhanced hydrogen bonding with water molecules. The results of this study showed that hydrogen bonds played an important role in improving the gelling properties of gelatin by γ-PGA.

Polyethylene‐Glycol‐Based Thermoreversible Biscarbamate Hydrogels and Metallogels Synthesized through Non‐Isocyanate Route

AbstractIn this work, an isocyanate free approach has been adopted to synthesize hydroxy urethanes exhibiting self‐assembling properties. Polyethylene glycol (PEG) end capped with glycerol carbonate was subjected to aminolysis with aliphatic mono amines to yield bishydroxyl urethanes. The amphiphile thus obtained exhibited gelation in water to yield thermoreversible hydrogels. The hydroxyl groups of urethane bond initiated self‐assembly through intra and intermolecular hydrogen bonding between the secondary hydroxyl groups and amide group of the biscarbamate moiety and this was exemplified by Fourier transform infrared spectroscopy (FT‐IR) studies. Differential scanning calorimetry (DSC) revealed that gel melting temperatures are influenced by the length of aliphatic mono amines. Orientation of the gelator molecules and the mode of aggregation of molecules into sheets during the self‐assembly was confirmed by XRD studies and field emission scanning electron microscopy (FE‐SEM). Visco‐elastic properties of resulting PEG‐hydrogels were studied by rheology. In addition, incorporation of metal ions increased the mechanical strength and thermal stability, which was assisted by complex formation between the metal ions and gelator molecules. One of the metal ion responsive hydrogel was capable of entrapping vitamin B12 and the slow release of drug molecule triggered in acidic pH was demonstrated.

Preparation of high viscoelastic emulsion gels based on the synergistic gelation mechanism of xanthan and konjac glucomannan

In the study, a facile methodology for fabricating high viscoelastic emulsion gels has been proposed, which is based on the synergistic gelation mechanism of xanthan and konjac glucomannan (KGM). The effects of preparation strategies, oil contents and simulated sterilization conditions (121 °C, 30 min) on the emulsion gels were systemically investigated. All emulsion gels exhibited similar gelation temperature (Tgel) and gel melting temperature (Tmelt), indicating that the gelling continuous phase played a predominant role in the emulsion gel formation. However, the viscoelasticity of the emulsion gels was different depending on preparation strategies and oil phase fractions. When a low concentration of tween-80 (<1.0 %, v/v) was incorporated during emulsification process, the resultant emulsion gels showed satisfactory thermal stability and could undergo simulated sterilization conditions without discernible oil droplet coalescence observed. Conclusively, these results demonstrate the potentials of co-utilization of xanthan and KGM in designing novel food products.

Physical Properties of Beeswax, Sunflower Wax, and Candelilla Wax Mixtures and Oleogels

AbstractTo be able to tailor and optimize the physical properties of oleogels for various food applications, more information is needed to understand how different gelators interact. Therefore, the objectives of this study were to evaluate the interactions between binary mixtures of beeswax (BW), candelilla wax (CLW), and sunflower wax (SFW) in pure form as well as in 5% wax oleogels made with soybean oil, in terms of their crystallization and melting properties, crystal morphology, solid fat content, and gel firmness. CLW:BW mixtures had eutectic melting properties, and oleogels from these mixtures with 40:60 to 90:10 CLW:BW were firmer compared to oleogels made with one wax. The main components in SFW and BW appeared to cocrystallize or crystallize at the same temperature, but nonlinear changes in melting point and solid fat content profile of oleogels prepared with the mixed waxes indicated that SFW dominated oleogel formation. In addition, oleogels prepared with mixtures of SFW and BW had lower firmness compared to oleogels prepared with one wax, indicating an incompatibility between the two waxes. The main wax components in SFW and CLW never cocrystallized, and low levels of CLW appeared to prevent SFW from forming a crystalline platelet network. This resulted in low firmness of oleogels made from mixtures of 90:10 to 60:40 SFW:CLW compared to oleogels prepared with one wax. However, the firmest oleogels of all mixtures were made from 10:90 SFW:CLW. Changes in gel firmness and melting properties with mixed wax oleogels were likely to be due to changes observed in the crystal size and morphology. In addition, the firmest gels were shown to result from mixtures that were predicted to have &gt;40% hydrocarbon content, and a high hydrocarbon to wax ester ratio, but minor components such as free fatty acids and fatty alcohols may have also influenced firmness.

Comprehensive Data of P53 R282 Gene Mutation with Human Papillomaviruses (HPV)-Associated Oral Squamous Cell Carcinoma (OSCC).

Alterations of the P53 gene and human papillomavirus (HPV) infection are associated with development of oral squamous cell carcinoma (OSCC). We aimed to identify mutation of P53 exon 8 codon 282 in OSCC and correlate these with HPV infection as well as histopathological grade of OSCC. Samples of known HPV infection status were studied including oral lesion cells, formalin-fixed paraffin embedded (FFPE) tissues from OSCC and exfoliated oral cells of matched age-sex controls. P53 exon 8 mutation was detected using the polymerase chain reaction (PCR). Mutation of codon 282 was identified by allele-specific oligonucleotide typing (ASO) using EvaGreen real-time PCR. The PCR products were analyzed by gel electrophoresis and melting curve analysis. Mutation of P53 exon 8 was seen in 81.7% and 69.6% of FFPE OSCC tissues and oral lesion cells, respectively. This was significantly higher than in controls (16.7%). Frequency of mutation did not differ between HPV-positive samples (62.5% and 81.8% in oral lesion cells and FFPE tissue samples, respectively) and HPV-negative samples (73.3% and 81.5% in oral lesion cells and FFPE tissue samples, respectively). This finding is similar to P53 codon 282 mutation that was found only in FFPE tissues (35.0%) and oral lesion cells (32.6%) from both HPV-positive and negative OSCC. Interestingly, frequency of mutation was higher in well-differentiated OSCC with HPV-infection (28.1%) than without HPV (14.8%). This result demonstrated a mutation hot spot in P53 associated with oral carcinogenesis and might be useful to guide chemotherapeutic modality for HPV-associated OSCC in northeast Thailand.

Thermo-Reversible Hybrid Gels Formed from the Combination of Isotactic Polystyrene and [Fe(II) (4-Octadecyl-1,2,4-Triazole)3(ClO4)2]n Metallo-Organic Polymer: Thermal and Viscoelastic Properties.

Nano-sized one-dimensional metallo-organic polymers, characterized by the phenomenon of spin transition, are excellent candidates for advanced technological applications such as optical sensors, storage, and information processing devices. However, the main drawback of this type of polymers is their fragile mechanical properties, which hinders its processing and handling, and makes their practical use unfeasible. To overcome this problem, in this work, hybrid thermo-reversible gels are synthesized by combination of a metallo-organic polymer and isotactic polystyrene (iPS) in cis-decaline. A detailed investigation of the thermal and viscoelastic properties of the hybrid gels, in terms of iPS and metallo-organic polymer concentration is performed by means of differential scanning calorimetry and oscillatory rheology, respectively. From the analysis of the thermal properties, three transitions have been determined upon heating: Monotectic transition of the iPS gel, melting of the iPS gel, and melting of the metal-organic polymer gel, which suggest that the gels of the two polymers are formed independently in the hybrid gel, as long as the two polymers are in concentrations above the corresponding critical gelation concentrations. Results regarding viscoelastic properties and morphology confirmed that hybrid gels consisted of an interpenetrated network of polymer gels, formed by iPS and metallo-organic poymer gels growing independently.

Comparison of gelling properties and flow behaviors of microbial transglutaminase (MTGase) and pectin modified fish gelatin

The gelling and structural properties of microbial transglutaminase (MTGase) and pectin modified fish gelatin were compared to investigate their performances on altering fish gelatin properties. Our results showed that within a certain concentration, both MTGase and pectin had positive effects on the gelation point, melting point, gel strength, textural, and swelling properties of fish gelatin. Particularly, low pectin content (0.5%, w/v) could give fish gelatin gels the highest values of gel strength, melting temperature, and hardness. Meantime, flow behavior results showed that both MTGase and pectin could increase fish gelatin viscosity without changing its fluid characteristic, but the latter gave fish gelatin higher viscosity. Both MTGase and pectin could increase the lightness of fish gelatin gels but decreases its transparency. More importantly, fluorescence and UV absorbance spectra, particle size distribution, and confocal microscopy results indicated that MTGase and pectin could change the structure of fish gelatin with the formation of large aggregates. Compared with MTGae modified fish gelatin, pectin could endow fish gelatin had similar gel strength, thermal and textural properties to pig skin gelatin.

Polysaccharide Coating of Gelatin Gels for Controlled BSA Release.

Self-assembly of natural polymers constitute a powerful route for the development of functional materials. In particular, layer-by-layer (LBL) assembly constitutes a versatile technique for the nanostructuration of biobased polymers into multilayer films. Gelatin has gained much attention for its abundance, biodegradability, and excellent gel-forming properties. However, gelatin gels melt at low temperature, thus limiting its practical application. With respect to the above considerations, here, we explored the potential application of gelatin gels as a matrix for protein delivery at physiological temperature. A model protein, bovine serum albumin (BSA), was encapsulated within gelatin gels and then coated with a different number of bilayers of alginate and chitosan (10, 25, 50) in order to modify the diffusion barrier. The coated gel samples were analyzed by means of Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) and confocal Raman spectroscopy, and it was found that the multilayer coatings onto polymer film were interpenetrated to some extent within the gelatin. The obtained results inferred that the coating of gelatin gels with polysaccharide multilayer film increased the thermal stability of gelatin gels and modulated the BSA release. Finally, the influence of a number of bilayers onto the drug release mechanism was determined. The Ritger-Peppas model was found to be the most accurate to describe the diffusion mechanism.

The effects of amino acids on the gel properties of potassium iota carrageenan

The effects of positive and neutral charged amino acids (Arginine (Arg) and Glutamine (Gln)) on the gel properties and microstructure of potassium iota Carrageenan (KIC) were investigated. The findings suggested that the gel melting temperature (Tg) of KIC gels increased from 36 °C to 43 °C with the Gln concentration increasing, and the elastic modulus (G’) increased as well. However, with the increase Arg concentration, Tg and G’ of gels increased gradually and then decreased, and the maximum Tg (46 °C) and G’ were obtained at concentrations of 0.05% Arg. LF-NMR results showed that the addition of Gln decreased the position of T23, indicating a negative effect of Gln on the water holding capacity and water mobility of KIC gels, while the addition of Arg increased the position of T23, showing the opposite effect. Compared to the pure KIC and Gln-KIC (GKIC), Arg-KIC (AKIC) showed a more regular network structure and a stronger water holding capacity. In addition, few chemical bonds were formed between amino acid and KIC. It is electrostatic attraction between KIC and Arg and the crowding of Gln that contributed to better gel properties. Taken together, our results provide insights for the further application of GKIC or AKIC gels in food and medical applications.

Effect of extraction temperature on the gelling properties and identification of porcine gelatin

High-performance liquid chromatography (HPLC) and linear-ion trap (LTQ)/Orbitrap high-resolution mass spectrometry were used to identify porcine gelatins extracted at different temperatures. Moreover, shear stress rheological detection techniques and a texture analyzer were applied to evaluate the gelling properties of various porcine gelatins. The results showed that, with an increased in the extraction temperature, the gel strength, gelation point and melting point of the porcine gelatin gradually decreased, indicating that the temperature significantly affected the gelling properties of gelatin. HPLC- LTQ/Orbitrap high-resolution mass spectrometry indicated that porcine gelatins prepared at different extraction temperatures also showed internal uniqueness, namely, diverse marker peptides for gelatin identification. Compared with the established theoretical sequence fragment database of porcine gelatin and bovine gelatin, 64, 74 and 71 tryptic porcine peptides in gelatins were extracted at 55 °C, 65 °C and 75 °C, respectively. Notably, regardless of the extraction temperature, 47 common peptides were detected in the tryptic hydrolysates of porcine gelatins. Using these common tryptic peptides can effectively improve the accuracy of the porcine gelatin identification.

Mitochondrial genome sequence variation as a useful marker for assessing genetic heterogeneity among Cyclospora cayetanensis isolates and source-tracking

BackgroundCyclospora cayetanensis is an important enteric pathogen, causing diarrhea and food-borne cyclosporiasis outbreaks. For effective outbreak identification and investigation, it is essential to rapidly assess the genetic heterogeneity of C. cayetanensis specimens from cluster cases and identify the likely occurrence of outbreaks.MethodsIn this study, we developed a quantitative PCR (qPCR) targeting the polymorphic link region between copies of the mitochondrial genome of C. cayetanensis, and evaluated the genetic heterogeneity among 36 specimens from six countries using melt curve, gel electrophoresis, and sequence analyses of the qPCR products.ResultsAll specimens were amplified successfully in the qPCR and produced melt peaks with different Tm values in the melt curve analysis. In gel electrophoresis of the qPCR products, the specimens yielded bands of variable sizes. Nine genotypes were identified by DNA sequencing of the qPCR products. Geographical segregation of genotypes was observed among specimens analyzed, which could be useful in geographical source-tracking.ConclusionsThe length and nucleotide sequence variations in the mitochondrial genome marker allow rapid assessment of the genetic heterogeneity among C. cayetanensis specimens by melt curve, gel electrophoresis, or DNA sequence analysis of qPCR products. The sequence data generated could be helpful in the initial source-tracking of the pathogen.

Functional Characteristics of Ultraviolet-Irradiated Tilapia Fish Skin Gelatin.

Studies were undertaken to investigate the effects of ultraviolet (UV) irradiation on the gel strength, color, thermal properties, protein molecular masses, and functional groups of commercially available fish gelatin samples. Commercially available tilapia skin gelatin powder was used as the raw material to investigate the functional properties of fish skin gelatin powder treated with UV irradiation for different durations (0–6 h). The functional properties of fish gelatin and the optimum irradiation treatment conditions were determined through gel strength testing, color characterization, differential scanning calorimetry, sodium dodecyl sulfate polyacrylamide gel electrophoresis, Fourier transform infrared (FTIR) spectroscopy, and Raman spectroscopy. UV irradiation treatment increased gel strength and thermal stability, and significantly degraded the macromolecules. FTIR and Raman spectroscopy data indicated that UV irradiation treatment did not significantly change the molecular structure of fish gelatin powder, but these methods could discriminate the molecular structure of gelatin from various sources. Irradiation for 2 h yielded the highest gel strength and melting peak temperature, and the lowest chromatic aberration.

Consolidated Melting Gel Coatings on AZ31 Magnesium Alloy with Excellent Corrosion Resistance in NaCl Solutions: An Interface Study.

Magnesium alloys, with a density two-thirds that of aluminum, are very attractive for the industry. However, these alloys are extremely susceptible to corrosion in the presence of aggressive electrolytes such as NaCl solutions. Here, we designed hybrid coatings obtained by the consolidation of organically modified polysilsesquioxanes called "melting gels" for the corrosion protection of AZ31 magnesium alloy in NaCl solutions. The main focus was to study the interaction between coatings and substrate and the influence of the coating thickness on the final properties. Micro-scratch tests, adhesion by tape tests, confocal Raman microscopy, SEM-EDS, and ToF-SIMS indicate good adhesion of coatings based on the interaction of melting gels and substrate. These measurements indicate the presence of the Si-O-Mg bonds between the substrate and coatings. Electrochemical results show very low current densities (10-13 A cm-2) without any breakdown potential and impedance values of 1010 Ω cm2.

Novel hard capsule prepared by tilapia (Oreochromis niloticus) scale gelatin and konjac glucomannan: Characterization, and in vitro dissolution

A novel material for making capsules was prepared with tilapia scale gelatin and konjac glucomannan (KGM). Rheological behaviors of gelatin with KGM at different levels (0.1%, 0.15%, and 0.2%, w/w) were investigated. The highest values (P < 0.05) of gel strength (518.33 ± 6.17 g) and melting temperature (39.7 ± 0.11 °C) could be observed at the gelatin solution with 0.15% (w/w) KGM. The tensile strength (TS) and elongation at break (EAB) of the films increased with the increasing of KGM. The water contact angle was enhanced with the increasing of KGM, indicating the decrease of the film hydrophilicity. XRD and FTIR showed the interactions between gelatin and KGM. Gelatin solution with 0.15% KGM is suitable for preparing capsules. Drug dissolution test in vitro showed the shell rupture time is in range of 3–5 min, and 80% of the drugs were released within 10 min. Therefore, the composite materials made of tilapia scale gelatin and KGM can be utilized for hard capsules.

Fabrication of Crystalline Gel Fibers By Electrospinning

The purpose of this research is to develop a new material with a combination of functions of gel and fiber using electrospinning. Gel has a three-dimensional network structure that is usually synthesized by crosslinking the monomers in various ways. It is widely used in hygiene, cosmetic, fragrance, medicine and many others fields. Multi-functions like water absorbability, biocompatibility and flexibility, can be designed and given depending on the chemical structure of monomers and crosslinkers. We developed crystalline gels with high performance via introducing crystalline side chains into the three-dimensional network structure. There are many kinds of crosslinks to form three-dimensional network structure, such as covalent bonds, Coulomb force, hydrogen bonds, coordination bonds, bonds through entanglements, etc. Among these crosslinks, covalent bonds are most suitable for preparing high performance gels. We use photopolymerization to synthesize crystalline gels because it offers advantages including rapid curing rate, minimal heat production and temperature independence, compared to other polymerization techniques. Photopolymerization is a light-based technique that uses to initiate and propagate a polymerization reaction. To create crosslinked gels, at least three reactive components are required: photoinitiator, monomer and crosslinking agent. A photoinitiator creates free radicals upon exposure to specific wavelengths of light, and then initiates polymerization of monomer and crosslinking agent through a photolytic mechanism. In this research, our aim is to develop a novel gel fiber with a combination of functions of gel and fiber, and ultimately to develop gel nonwoven fabric. The gel nonwoven fabric is expected to have the properties such as water absorbability, biocompatibility, flexibility, and also air permeability, filtration and heat retention. The gel nonwoven fabric could be a promising material for the application in medical treatment due to its air and moisture adjustment function to prevent soaking of wound. Gel has a three-dimensional network structure, it does not dissolve in an organic solvent to make gel solution, and it is also difficult to be heated to make gel melt. Before gelation, only the droplets but no fibers can be formed via electrospinning because of the too low viscosity of reactive solution. On the other hand, after full gelation, fibers cannot be formed either due to their insolubility and infusibility deriving from three-dimensional network structure. Therefore, the manufacture of gel fibers is difficult. In this study, electrospinning with pre and post polymerization treatment is used to make gel nonwoven fabric. We propose to use prepolymerized solution with appropriate viscosity to undergo electrospinning. Electrospinning is a method capable of relatively easily preparing nanofibers under a high voltage. An electrospinning device generally consists of a high voltage power supply, a cylinder, and a substrate. The principle of the electrospinning method is that when a positive high voltage is applied to the polymer solution, charges are collected on the droplet surface at the tip of the nozzle and repel each other, and the droplets coming out of the nozzle gradually become conical. When the repulsive force of charge exceeds the surface tension, the solution is jetted straight from the tip of the cone, gradually heading toward the grounded or negatively charged substrate while drawing a spiral. As the ejected solution stream becomes thinner, the surface charge density increases, so the charge repulsion increases and the solution flow is further elongated. Prepolymerized gel solution is set in cylinder, distance between substrate and nozzle is determined, extrusion amount is set, and voltage is given. We run experiments with the procedure of sticking aluminum foil to the metal plate and then spraying the prepolymerizted gel solution onto the board, finally taking aluminum foil off the board and applying postpolymeriation under UV light. SEM observation indicated the fiber diameter is about 400 nm.