Melon Cultivation Research Articles

Enhancing Post-Mining Lands’ Value through Sustainable Melon Cultivation: A Hydroponic Approach

This study investigates the potential of utilizing post-mining areas for sustainable melon (Cucurbitales, Cucurbitaceae, Cucumis) cultivation, focusing on premium melon production by PT. Indominco Mandiri within its Community Development and Empowerment program. A hydroponic system, which involves the cultivation of plants in a soilless medium with a nutrient solution, was employed due to its advantages in improving plant quality and yield. Findings demonstrate that melon thrives in ex-mining conditions, reaching harvesting maturity within 2.5 months, with fruits noted for their sweetness. Additionally, melons were found to be a rich source of vitamins A, K, C, B-6, E, niacin, and essential minerals including calcium, potassium, ferum, magnesium, phosphorus, sodium, and zinc, with low sodium and no fat content. This research underscores the hydroponic system’s potential in reclaiming post-mining lands, enhancing economic viability and fostering community development and empowerment. The implications of these findings extend to environmental rehabilitation and sustainable agriculture practices, offering a feasible and productive use of post-mining land globally.

GOURD GROWING EFFICIENCY USING SILICON-CONTAINING FERTILIZERS IN THE SOUTH OF UKRAINE

Purpose. To evaluate the effect of pre-sowing soaking of seeds on the plant performance and economic efficiency of watermelon and melon growing on drip irrigation in the South of Ukraine. Methods. Field, measuring, computational, comparative, and mathematical/statistical methods, analysis of economic efficiency. Results. Pre-sowing soaking of seeds in solutions of silicon-containing fertilizers was shown to help obtain early and full-bodied watermelon and melon seedlings, increase the length and number of lateral shoots, and improve fruit setting. Balanced plant nutrition contributed to high performance of watermelon and melon plants in the South of Ukraine. The highest yields of watermelon (23.4 t /ha) and melon (17.5 t/ha) fruits were harvested with 10% Quantum AquaSil solution, which increased the yield by 35.3 and 35.7%, respectively, compared to the untreated control. Silicon-containing fertilizers, 15% Bai-Si and Kelik PotassiumSilicon solutions, increased the fruit yield in watermelon fields by 22.5% and 19.7%, respectively, compared to the untreated control. The greatest economic effect of watermelon and melon growing was achieved by soaking seeds in 10% Quantum AquaSil solution prior to sowing; the profitability amounted to 122 and 101%, respectively. Conclusions. Pre-sowing soaking of seeds in silicon-containing fertilizer solutions contributed to high performance of plants and allowed increasing the economic efficiency of cultivation. The maximum percentage of fruit setting was recorded after seed treatment with 10% Quantum AquaSil solution. For example, this parameter was 67% in watermelon ‘Charivnyk’ and 87% in ‘Didona’ melon. In the south of Ukraine, the economic effect of outdoor watermelon and melon cultivation increased by 16,030–19,220 UAH/ha and 21,230–26,340 UAH/ha, respectively.

Ecological testing of melon varieties bred at the Bykovskaya Experimental Station

Relevance and methodology. Currently, due to changing weather conditions, the cultivation of melons and gourds is shifting to areas that were not previously engaged in melon growing. Therefore, the testing of available varieties in various environmental conditions is relevant. The purpose of this work is to evaluate melon varieties bred at the Bykovskaya melon breeding experimental station in various soil and climatic conditions. Three varieties of melon were used as the object of research. The tests were carried out in the conditions of the Volgograd region, the Krasnodar region and the Republic of Kazakhstan. During the research, the melon varieties were evaluated according to the following indicators: yield, duration of the growing season, dry matter content.Results. As a result of the research, it was revealed that the variety Harmonia was the most stable in terms of the duration of the growing season (75-79 days). A consistently high content of dry matter in all areas of the study was in the varieties Comet and Idyll from 12.0 to 15.0%. All varieties had a good tasting score. The average fruit weight of all melon varieties grown in Krasnodar and the Volgograd region was at the same level. The high average weight in the conditions of Kazakhstan was distinguished by the Idyll variety - 4.3 kg. The melon varieties Harmoniya and Idyllia have a consistently high yield in all three zones. The variety Kometa had the highest yield in the conditions of Kazakhstan - 16.3 t/ha. Thus, melon varieties that have been tested in different regions of cultivation have high quality indicators and yields and are suitable for growing marketable products in the regions of the study.

Use of water-soluble microfertilizers for growing meteor table variety watermelon

Relevance. In connection with the introduction of various types of chelated microfertilizers into production, it is necessary to develop new elements of varietal technology for the cultivation of melons and gourds in order to obtain stable yields in the arid conditions of the Volgograd Trans-Volga region. These studies will be relevant in this period of time.Material and technique. The object of research is watermelon, variety Meteor. We studied the types and methods of using chelate microfertilizers: Helaton Extra, Chelate B, Chelate Fe. Fertilizers were used for soaking seeds before sowing and for foliar treatment of plants during the growing season.Results. During the research period of 2019-2021, new forms of microfertilizers were studied. As a result of the research, new preparations for different methods of processing had a positive effect on the growth and development of table watermelon plants. The evaluation of the results obtained showed a positive effect of new microfertilizers on the germination energy and seed germination. The maximum growth of lashes was recorded when seeds were soaked before sowing: Helaton Extra preparation and foliar feeding of plants during the growing season with microfertilizer boron chelate. The best results of increasing the leaf surface were obtained in variants with the use of Helaton Extra microfertilizer for soaking seeds and treating plants on vegetative organs. Accordingly, the highest yield was obtained when using Helaton Extra fertilizer for seed treatment before sowing and spraying plants during the growing season. The output of marketable products was noted at a fairly high level. The average weight of the fetus in all studied variants ranged from 5.9 kg to 7.8 kg. The length of the growing season in all variants increased slightly. Comparative analysis of growth processes and productivity of watermelon variety Meteor when using new forms of water-soluble fertilizers for seed soaking and foliar treatment of plants showed greater efficiency from their use.

Influence of chelated microfertilizers on growth processes and yield of table watermelon under agricultural conditions of the Volgograd Volga region

Relevance. The Volgograd region is a region of risky farming. In the spring-summer period, the cultivated crop (melons) is often exposed to stress factors: spring frosts, abnormal heat, temperature changes, which negatively affects the growth and development of plants. In this regard, it is necessary to develop new adaptive agrotechnical methods for the cultivation of melons and gourds in order to obtain stable yields of high quality.Material and methodology. The object of research is watermelon, variety Meteor. Water-soluble fertilizers were studied – Helaton Extra and Aquarin vegetable for soaking seeds before sowing and foliar treatment of plants during the growing season.Results. During the research period of 2019-2021, new types of microfertilizers were studied.When using preparations Aquarin vegetable and Helaton Extra for different methods of processing, a positive effect on the growth and development of table watermelon plants, as well as on the increase in the leaf plate, was noted. Helaton Extra for soaking seeds and treating plants had the maximum impact on the growth processes of early table watermelon. As a result of the research, the yield of the studied variety also increased. The average weight of the fetus in all variants increased compared to the control variant by 2.7-5.3%. The length of the growing season in all variants increased from 2 to 4 days, which favorably affected the increase in yield. Comparative analysis of growth processes and productivity of watermelon variety Meteor when using new forms of water-soluble fertilizers for seed soaking and foliar treatment of plants showed greater efficiency from their use.

Melon (Cucumis melo L.) Fruit Yield under Irrigation and Mycorrhiza Conditions

The size and quality of the melon fruit yield depend on the cultivar, climatic and agronomic factors. A three-year field experiment investigated the effect of arbuscular mycorrhizal fungi (AMF) application and irrigation (IR) on the fruit yield of melon (Cucumis melo L. var. saccharinus Naud.) cultivars. The study was conducted on a certified organic farm located in south-eastern Poland (51.36° N, 22.83° E). The factors of the experiment were as follows: cultivar (Melba, Emir F1, Seledyn F1, Oliwin) and cultivation method (AMF and IR; AMF and non-IR; non-AMF and IR; non-AMF and non-IR as control). The dry matter (%), soluble solid (%), total sugar and reducing sugar (% fresh weight, FM), L-ascorbic acid (mg · 100 g−1 FM), and carotenoid (mg · 100 g−1 FM) contents of the fruit were determined. The highest total and marketable fruit yields were obtained using AMF and IR. Fruit from the AMF series (IR and non-IR) had the most carotenoids (respectively: 801.5 and 788.8 µg · 100 g−1 FM). The fruits of the AMF and IR plants contained the most total sugars (5.98%) and reducing sugars (2.91%) compared to the others. The control plants had the lowest number of marketable fruit, total and marketable fruit yield, and accumulated the least L-ascorbic acid, total sugars and reducing sugars. We suggest that AMF and IR can be recommended as a practical agronomic solutions for the field cultivation of melon under temperate climate conditions.

Photosynthesis by Leaf Age and Fruit Characteristics by Fruiting Nodes in Vertical and Hydroponic Cultivation of Oriental Melon Applied with Air Duct for High-temperature Season

This study was conducted to apply with an air duct for the cooling and a utilizing cultivating method that uses the fruiting node and the defoliation to the high-temperature vertical and hydroponic cultivation of the oriental melon. The lower fruiting node (LF) was to remove all third vines generated from 5 nodes of a secondary vine. The higher fruiting node (HF) was fruiting on the third vine generated from a first node of the third vine. The direction of the stem string; upward (UW), downward (DW). Four treatment conditions were applied with the LF-UW, LF-DW, HF-UW (control), and HF-DW. The leaf age of melon leaves was measured for photosynthesis at 3 days intervals, and the fruit characteristic was conducted on 79 fruits in each treatment. The photosynthesis rate steadily increased after leaf development, reaching 20.8 µmol CO2·m-2·s-1 on the 10 days, gradually increasing to 21.3 µmol CO2·m-2·s-1 on the 19 days, and reaching 23.4 µmol CO2·m-2·s-1 on the 32 days. After that, it lowered to 16.8 µmol CO2·m-2·s-1 on the 38 days and dropped significantly to 7.6 µmol CO2·m-2·s-1 on the 47 days. As a result of the fruit characteristics by fruiting nodes, the treatments of the fruit length was 12.6-13.4 cm, respectively, which was significant, and the fruit width was 7.9- 8.6 cm, respectively, was not significant. The soluble content ranged from 12.9 to 15.7°Brix, and the significance of all treatments, and higher than of LF-DW and HF-UW. The photosynthesis rate of melon leaves was good until 32 days after leaf development, but after that, the rate decreased. As for fruit quality, it was conformed that melons can be cultivated at the LF because the fruit enlargement and soluble content dose not decrease even when set at the LF. Results indicated that those can be used for LF and defoliation in the development of vertical and hydroponic cultivation method in high-temperature season.

Organic Fertilizer and Technical Efficiency in Melon Cultivation in Wonogiri, Central Java

Melon (Cucumis melo L.) is a potential seasonal fruit crop. In melon cultivation, farmers combine various inputs. Combined inputs such as land, seeds, organic fertilizers, inorganic fertilizers, pesticides, labor. Organic fertilizer is now a promising method for sustainable agriculture where it is most affordable compared to synthetic fertilizers. The objectives of this study were (1) to determine the technical efficiency (ET) of melon farming, (2) to determine the effect of manure on melon production. (3) To determine the effect of age, formal education and farmer experience on the use of manure. Giriwoyo sub-district was chosen as the research location because it has the largest area of harvested land and the highest amount of melon farming production in Wonogiri Regency. Selection of farmer samples using accidental sampling technique. The number of respondents was 60 farmers. The conclusion from the results of the study that (1) the average technical efficiency of melon farmers is 96.7% classified as high criteria, (2) manure has a positive effect on melon production, thus the use of manure can increase melon production which will further increase technical efficiency. (3) Age, education and experience have a positive effect on the use of manure. The suggestions given are (1) to increase technical efficiency can be done by adding manure and (2) increasing the use of manure can be done by increasing age, education and experience. Increasing age as a proxy for information, namely by adding information, increasing knowledge with training and increasing experience can be improved by apprenticeship activities with other parties. Experience is a key factor to increase the use of manure.

Organic Fertilizer and Technical Efficiency in Melon Cultivation in Wonogiri, Central Java

Organic Fertilizer and Technical Efficiency in Melon Cultivation in Wonogiri, Central Java

Multiscale Deepspectra Network: Detection of Pyrethroid Pesticide Residues on the Hami Melon.

The problem of pyrethroid residues has become a topical issue, posing a potential food safety concern. Pyrethroid pesticides are widely used to prevent and combat pests in Hami melon cultivation. Due to its high sensitivity and accuracy, gas chromatography (GC) is used most frequently for detecting pyrethroid pesticide residues. However, GC has a high cost and complex operation. This study proposed a deep-learning approach based on the one-dimensional convolutional neural network (1D-CNN), named Deepspectra network, to detect pesticide residues on the Hami melon based on visible/near-infrared (380-1140 nm) spectroscopy. Three combinations of convolution kernels were compared in the single-scale Deepspectra network. The convolution group of "5 × 1" and "3 × 1" kernels obtained a better overall performance. The multiscale Deepspectra network was compared to three single-scale Deepspectra networks on the preprocessing spectral data and obtained better results. The coefficient of determination (R2) for lambda-cyhalothrin and beta-cypermethrin was 0.758 and 0.835, respectively. The residual predictive deviation (RPD) for lambda-cyhalothrin and beta-cypermethrin was 2.033 and 2.460, respectively. The Deepspectra networks were compared with two conventional regression models: partial least square regression (PLSR) and support vector regression (SVR). The results showed that the multiscale Deepspectra network outperformed the other models. It was found that the multiscale Deepspectra network could be a novel approach for the quantitative estimation of pyrethroid pesticide residues on the Hami melon. These findings can also provide an effective strategy for spectral analysis.

Analysis Of The Factors Influencing The Production Of Meloon Farming Business In Tanjung Kamal Village, Mangaran District, Situbondo District

Melon plants are one of the top priority crops that need special attention among other horticultural crops, because these plants have their own charms, for example the price is relatively high, has a good taste, smells good and is refreshing. These plants are suitable for development in the Situbondo area because soil and climatic conditions in accordance with the conditions of growing these plants. The level of production of melon farming is determined by the cultivation, because the more intensive the melon cultivation, the higher the production.With a fairly good production results it will be able to increase revenue. The purpose of this study was to analyze the effect of land area variables, seed variables, fertilizer variables, and labor variables on the amount of melon production in Tanjung Kamal Village, Mangaran District, Situbondo Regency. Methods of data analysis using Cobb Douglas production function analysis. The results of this study indicate that the variables of land area and seeds have a significant effect on melon production, while the variables of fertilizer and labor. work does not have a significant effect on melon production.

Identification of main fungal disease from hydroponic melon in greenhouse

Hydroponic melon is a method of cultivating melons and grown in a greenhouse. The melon cultivation has problems, namely plant diseases, even in a closed greenhouse. The melon plant disease caused huge losses in melon cultivation until the melon plants died. The purpose of this descriptive study was to identify the cause of the melon plant disease. Samples of sick melon plants were taken and grown on PDA media in the laboratory. The fungus that grows is identified based on the literature. The identification results showed that the main disease causing hydroponic melon plants in the greenhouse was the fungus Fusarium oxysporum f.sp. melonis. The fungi have crescent-shaped macroconidia with 1-3 septa, hyaline, thick walls, and form chlamydospores that are spherical in intercalary and hyaline.

Incidence of Virus Diseases in Major Cultivated Areas of Watermelon and Melon in Chungbuk Province

To investigate the incidence status of viruses in major cultivated areas of watermelon and melon in Chungbuk Province, samples were collected from 2020 to 2021 in vinyl greenhouse of Jincheon and Eumseong and examined for virus infection using reverse transcription polymerase chain reaction. Of the six viruses on watermelon that was analyzed in this study, watermelons were infected with cucumber mosaic virus (CMV), watermelon mosaic virus (WMV), cucumber green mottle mosaic virus (CGMMV), and cucurbit aphid-borne yellows virus (CABYV). The incidence rate of CMV was 20.9–35.0%, WMV 0.4–15.8%, CGMMV 1.6–38.5%, and CABYV was 3.5–3.7% from 2020 to 2021. But strangely, there were no incidence of zucchini yellow mosaic virus and cucurbit chlorotic yellows virus (CCYV) during investigation. From this result, we knew the major virus was CGMMV on watermelon in Chungbuk Province. Molecular diagnosis assays of the two melon viruses, showed that melons were infected with CABYV and CCYV from 2020 to 2021. The incidence rate of CABYV was 53.9–92.2% and CCYV was 2.7–20.8%. The incidence of CABYV was high in melon cultivation of Jincheon and Eumseong, Chungbuk. Afterwards, it is necessary to establish a control management strategy for reduce the incidence of CABYV. Furthermore, we must pay attention that of CCYV even if the incidence was low.

Alfisol Soil Fertility Before Planting and After Harvest as Meloon Planting Media with Bioboost Fertilization

Soil fertility is important in the cultivation process. Soil fertility is the potential of the soil to provide nutrients in sufficient quantities in available and balanced forms to ensure optimum plant growth and production. The current availability of nutrients in the soil has decreased as a result of continuous land use with the use of chemical fertilizers in the cultivation process, plus leaching and erosion by rainwater, not least in Alfisol soils. Therefore, fertilization with organic materials is carried out, namely the provision of Bioboost fertilizer. Bio boost is a biological fertilizer containing superior soil microorganisms, useful for increasing soil fertility as a result of soil biochemical processes. One of the applications of organic cultivation is the cultivation of melon (Cucumis melo L.) because melon is a plant that has high economic value and is profitable to be cultivated as a source of income for farmers. Based on this, a study was conducted to determine the nutrient status of Alfisol soil before planting and after harvesting which was used as a Melon plant with various concentrations of Bioobost fertilization. Furthermore, for the analysis of soil fertility, laboratory analysis was carried out by taking 1 kg of disturbed soil samples that had been air-dried and then analyzing soil properties, namely texture, pH (H2O), C-Organic (%), Nitrogen (%), P2O5 (ppm), K (cmol kg-1), Ca (cmol kg-1), Mg (cmol kg-1), Na (cmol kg-1), Na (cmol kg-1), CEC (cmol kg-1), and KB (%). The application of Bio boosts fertilizer showed an increase in the nutrient status of Alfisol soil as a Melon growing from low to moderate categories before planting to medium to high categories after harvest. The more concentration of Bioboost fertilizer given, the soil nutrient status also increases. The concentration of P4 (1100ml bio boost + 400ml water) gave the highest increase in nutrient status.
 Keywords: bioboost; soil-fertility; melon

The archaeobotany of Qaratepe, Azerbaijan 2nd–13th century

This paper presents the results of an archaeobotanical analysis of plant macro-remains recovered during excavations of a rural tepe site at Qaratepe, Azerbaijan, occupied during the Sasanian and Islamic periods between the 2nd and 13th centuries ad. The material derives from a 4 year Oxford University expedition which occurred between 2015 and 2018, ‘The Archaeological Exploration of Barda Project (AEB)’, established to investigate the provincial structure of the eastern Caucasus region in the Late Antique and early Islamic periods. Traditionally, archaeological practice in Azerbaijan has not embraced environmental archaeological techniques and despite the region’s importance to the understanding of early agriculture and the diffusion of crop species during the Islamic period, little archaeobotanical research has been conducted there to date. This assemblage therefore forms a rare and unique contribution to the field of archaeobotany in the Late Antique and Islamic periods in Azerbaijan and provides the first archaeobotanical evidence of crop husbandry at a rural settlement during these periods. In total, 8,676 carbonised plant remains representing a minimum of 60 species were recorded from 80 samples analysed, providing important insights into plant utilisation in Azerbaijan (Full taxonomic list available in on-line supplementary material (ESM)). Archaeobotanical evidence has revealed the range of crops cultivated and consumed at the site between the 2nd and 13th centuries. Results demonstrate that naked wheat (Triticum aestivum/durum/turgidum), barley (Hordeum vulgare), and broomcorn millet (Panicum miliaceum) were the primarily cultivated crops between the 2nd and 6th centuries, key crops that have been present in the Southern Caucasus for several millennia. The study has also identified the cultivation of rice (Oryza sativa), watermelon (Citrullus lanatus), and melon (Melo sativa) in the 13th century, indicating a change in agricultural production in the Islamic period and the introduction of several new cultivars and agricultural adaptions.

PENGARUH JENIS PUPUK ORGANIK DAN PENJARANGAN BAKAL BUAH TERHADAP PERTUMBUHAN DAN HASIL MELON

Utilization of organic matter as a plant nutrition can be used to increase the growth and yield of melon cultivation. Fruit thinning treatment can be used to improving fruit quality. This study aims to examine the effect of various organic fertilizers and fruit thinning on the growth and yield of melons. The study was conducted in Screen house Universitas Perjuangan Tasikmalaya from March to June 2021. The study used a factorial completely randomized design. First factor is type organic fertilizer consist of solid chicken manure, vegetable waste liquid fertilizer, and cow urine. Second factor is fruit thinning consist of 1 and 2 remaining ovules. Based on the research results, the interaction of vegetable waste with the remaining 1 ovule resulted in the highest number of leaves compared to other interactions. Chicken manure and vegetable waste with 1 and vegetable waste with 2 ovules produced the greenest leaves compared to other interactions. The application of organic fertilizer had no significant effect on plant biomass, root length, fruit diameter, fruit thickness, total dissolved solids, and productivity. Fruit thinning significantly affected to plant biomass, root length, fruit diameter, fruit thickness, fruit weight per plant, and productivity. 1 ovule was the best treatment compared to 2 ovules. Provision of vegetable waste with 1 and 2 ovules affects the growth and yield of melon plants. Fruit thinning by leaving 1 ovule effectively to growth and yield of melons.

STRATEGI PEMASARAN BUAH MELON HIDROPONIK (Studi Kasus Di Rasidin Veggies House, Kecamatan Patrang, Kabupaten Jember)

This study aims to 1) explain the melon cultivation in Rasidin Vegies House, 2) identify the marketing mix strategy in Rasidin Vegies House, 3) analyze the marketing strategy using SWOT framework. The method used to determine the research location is purposive method. The research is located at Rasidin Vegies House, Patrang Sub-d istric t , Jember District. This research used descriptive qualitative methods with case study techniques. The data obtained through are observation, in depth interview, and documentation.The result showed that 1)Rasidin Vegies House cultivates Honey Globe melon varieties with hydroponic drip irrigation system, 2) the 4P of marketing mix apllied are price, promotion, place, and product, 3) based on SWOT analysis gained are internal factors (strenght and weakness) and external factors (opportunity and threats). First, the strenght of Rasidin Vegies House’s cultivation is using hydroponic system. Second, the weakness found that is climatic condition in green house still haven’t optimal.Then, the opportunity is high-yielding variety in cultivation. But, this melon production has threat in market demand stock.

Experimental study of the process of drying melon slices in a chamber-convection dryer

This article discusses the design features of a chamber-convective drying installation, the principle of its operation and the results of experimental studies of drying ring-shaped melon slices. Based on the analysis of the results obtained, recommendations are given for the use of a drying plant in farms and dehkan farms specializing in the cultivation and processing of melons.

IoT of melon plant continuous diagnostic technique using an open chamber system measurement for photosynthesis and transpiration

Greenhouse growers can utilize techniques to measure the condition of crops, such as growth rate and wilting degree, in addition to environmental information such as temperature, humidity, and solar radiation. These measurements can serve as supplementary data for cultivation management decisions. Among them, photosynthetic rate is a crucial indicator for crop productivity and yield. In this study, we aimed to develop a system that continuously measures the photosynthetic rate, an essential factor in crop biomass production, using an open chamber methodwithin a melon cultivation greenhouse. The measured data is uploaded to thecloud, enabling real-time monitoring of melon photosynthesis throughout the cultivation process. The developed system was implemented at a low cost by utilizing a CO2 sensor, a singleboard microcontroller, and a cloudbased database service. With this system, the net photosynthetic rate and transpiration rate of melon plants could be continuously measured over an extended period and accessed anytime using a PC or smartphone. Additionally, concurrent measurements of melon plant weight and fruit weight allowed for visualizing the plant’s response to environmental factors, including changes in photosynthesis and transpiration rates due to weather conditions.

Abscisic acid and hydrogen peroxide regulate proline homeostasis in melon seedlings under cold stress by forming a bidirectional closed loop

Low temperatures severely limit melon cultivation and production in winter and spring. Cold stress can induce proline accumulation in melon seedlings. However, the accumulation mechanism is unclear and the way by which abscisic acid (ABA) and hydrogen peroxide (H 2 O 2 ) act as signaling molecules in the process remains unknown. We first identified key enzyme genes in the proline metabolism pathway, and identified six genes, namely, two CmP5CSs and one each of CmP5CR , CmOAT , ProDH , and CmP5CDH . Low temperature induced proline accumulation by up-regulating three synthase genes, CmP5CS1 , CmP5CR , and CmOAT , while suppressing the expressions of two catabolic genes, namely, CmProDH and CmP5CDH . Cold stress triggered ABA and H 2 O 2 synthase genes to activate their accumulation. Both H 2 O 2 and ABA pretreatments enhanced melon seedling’s cold tolerance, but the two behaved divergently in proline metabolism. Exogenous H 2 O 2 enhanced or maintained the aforementioned induction efficiency of low temperature. In contrast, ABA only induced CmP5CR and CmOAT expression, with no effect on CmP5CS1 , and synchronously induced CmProDH and CmP5CDH expressions, leading to a slight reduction in proline content. Alternatively, ABA controlled proline homeostasis under cold stress, at least in part, by inhibiting H 2 O 2 generation. Overall, the results suggest that ABA and H 2 O 2 formed a fine bidirectional closed loop to regulate proline homeostasis in melon seedlings. • Proline metabolism genes in melon were identified for the first time. • Cold induced proline accumulation by promoting CmP5CS1 , CmP5CR , CmOAT expression, and inhibiting CmProDH , CmP5CDH expression. • Exogenous H 2 O 2 induced proline and ABA accumulation for cold tolerance improvement. • ABA inhibited H 2 O 2 generation to keep proline homeostasis under cold stress.