The potential of 4-borono-2-[ 18F]fluoro- d,l-phenylalanine ([ 18F]FBPA), a fluorinated derivative of a target compound for boron neutron capture therapy, for melanoma imaging by positron emission tomography (PET) was studied using animal models. A high uptake of [ 18F]FBPA was found in murine B16 melanoma or in Greene's melanoma No. 179, a melanotic cell line in hamsters, for the first 6 h after injection. Whole body autoradiography using [ 18F]FBPA gave a clear image of the B16 tumor. The acid-insoluble 18F in the B16 increased to 27% by 6h, and most of the free 18F was detected as [ 18F]FBPA in both B16 and plasma. In the hamster models, No. 179 showed a 1.7 times higher uptake than amelanotic Greene's melanoma No. 178 at 6 h post-injection, although both melanomas indicated similar metabolic activities when examined by a tracer uptake study using l-[ 14C]methionine, 2-deoxy- d-[ 14C]glucose and [ 3H]thymidine. [ 18F]FBPA may be a very promising PET tracer for melanoma imaging.