Mediolateral Bending Research Articles

Kinetics of Symmetrical Versus Asymmetrical In-Phase Gaits During Arboreal Locomotion.

Quadrupedal animals traveling on arboreal supports change aspects of locomotion to avoid slipping and falls. This study compares locomotor biomechanics in two small mammals: first, the gray short-tailed opossum (Monodelphis domestica) predominantly trots, which is a symmetrical gait. The second species, the Siberian chipmunk (Tamias sibiricus), primarily bounds or half-bounds. Trotting and bounding differ fundamentally in three aspects: location and timing of hand and foot placement; in the way that the trunk bends (trotting, mediolateral bending; bounding, flexion, and extension); and in the dynamics of the center of mass. Both species ran on a flat track and a 2 cm diameter cylindrical track, instrumented with a force plate or pole. For bounding chipmunks, the force pole was modified to measure force only on the right side. We measured speed, duty factor, and force, and calculated vertical, braking, propulsive, and net mediolateral impulses. Vertical and fore-aft impulses were different between trotting opossums and bounding chipmunks, but between trackway types, these impulses were similar within each species. The modifications used by each species to travel on arboreal supports were similar, except in one important respect. Net mediolateral impulse in opossums changed from laterally directed on the flat trackway to medial on the arboreal. But in chipmunks, these impulses on the flat track were medially-directed, and on the arboreal track, the amount of variability was substantially greater. We conclude that chipmunks-and perhaps any bounding animal-are less consistent from stride to stride in their locomotion. This inconsistency requires constant medial and lateral impulses to correct their trajectory when traveling on arboreal surfaces.

Fossil and modern penguin tarsometatarsi: cavities, vascularity, and resilience

AbstractPenguin tarsometatarsi are shortened and flattened, and studies devoted to the internal characteristics of these composite bones are very limited. Therefore, we present here a comprehensive, x‐ray‐microscopy‐based analysis based on tarsometatarsi of Eocene stem Sphenisciformes from Seymour Island (Antarctic Peninsula) as well as recent Aptenodytes forsteri, A. patagonicus, and Pygoscelis adeliae penguins. Our study focuses on four aspects: size variability of the medullary cavities, vascularization patterns with emphasis on diaphyseal vessels, cross‐sectional anisotropy, and diaphyseal resistance to bending forces. Small‐sized Eocene penguins (Delphinornis and Marambiornopsis) show well‐developed tarsometatarsal medullary cavities, whereas the cavities of “giant” early Sphenisciformes are either smaller (Palaeeudyptes) or show a conspicuous intermetatarsal size gradient (Anthropornis). Extant penguins exhibit a decrease in cavity dimensions as their body size increases. Distributional tendencies of primary diaphyseal nutrient foramina are quite similar in the smaller Delphinornis, Marambiornopsis, and extant Pygoscelis on one side and in Palaeeudyptes and extant Aptenodytes on the other. Anthropornis shows a unique, plesiomorphic pattern with a prevalence of plantar blood supply to the metatarsals. The diaphyseal nutrient canals diverge in orientation, some obliquely away from the proximal part, others with disparate trajectories. Cross‐sectional anisotropy along the tarsometatarsal shaft generally appears to be rather low. Clustering of coherency curves along certain tarsometatarsal segments may reflect a selection process that exerts a significant influence within biomechanically crucial sections. Diaphyseal resistance to mediolateral bending forces is explicitly more efficient in extant penguins than in Eocene Sphenisciformes. This can be interpreted as an adaptation to the waddling gait of extant penguins.

CHARACTERIZING SPINAL LOAD-DISPLACEMENT BEHAVIOUR USING REPRESENTATIVE COMPONENTS

AbstractObjectivesSpinal stiffness and flexibility terms are typically evaluated from linear regression of experimental data and are then assembled into 36-element matrices. Summarising in vitro test results in this manner is quick, computationally cheap and has the distinct advantage of outputting simple characteristic values which make it easy to compare results. However, this method disregards many important experimental features such as stiffening effects, neutral and elastic zones magnitudes, extent of asymmetry and energy dissipation (hysteresis). Alternatives to the linear least squares method include polynomials, separation of the load-displacement behaviour into the neutral and elastic zones using various deterministic methods and variations on the double sigmoid and Boltzmann curve fits. While all these methods have their advantages, none provide a comprehensive and complete characterisation of the load-displacement behaviour of spine specimens. In 1991, Panjabi demonstrated that the flexion-extension and mediolateral bending behaviour of functional spinal units could be approximated using the viscoelastic model consisting of a nonlinear spring in series with a linear Kelvin element. Nowadays viscoelastic models are mainly used to describe creep and stress relaxation, rather than for cyclic loading. The aim of this study was to conclusively prove the viscoelastic nature of spinal behaviour subject to cyclic loading. Being able to describe the behaviour of spine specimens using springs and dampers would yield characterising coefficients with recognisable physical meaning, thus providing an advantage over existing techniques.MethodsSix porcine isolated spinal disc specimens (ISDs) were tested under position and load control. Visual inspection of the load-displacement graphs from which the principal terms of the stiffness and flexibility matrices are derived suggest that the load-displacement behaviour could be idealised by a nonlinear spring system with damping. It was hypothesised that the contributions arising from non-linear spring-like behaviour and damping could be separated for each of the principal load-displacement graphs.ResultsThe principal elements from position and load control tests were plotted with load on the vertical axis and displacement on the horizontal axis, and a polynomial representing spring force was fitted to the data. This polynomial was subtracted from the experimental data. The remainder - representing damping force - was plotted against displacement and velocity and compared to idealised plots for different types of damping behaviour. Applying this reasoning to the six principal load-displacement plots obtained from stiffness and flexibility tests revealed that four of six exhibit behaviour which is a combination of a nonlinear hardening spring and viscous damper. However, for flexion-extension and mediolateral bending, the damping behaviour is more akin to coulomb damping with viscous influences, characterised by a steep central linear region and near horizontal regions at the extremes. These trends were common amongst all six specimens within the study sample size.ConclusionsThe unique approach described here has allowed the characterisation of the load-displacement behaviour of ISDs in terms of characteristic mass-spring-damper systems. This is an important development which allows experimental findings to be framed in terms of mechanical contributions to a given load-displacement behaviour.Declaration of Interest(b) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported:I declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

Comparação biomecânica entre placa de TPLO modificada, placa bloqueada e construção placa-pino intramedular aplicadas medialmente em modelo de fratura proximal em tíbias sintéticas caninas

ABSTRACT The aim of this study was to develop a modified tibial plateau leveling osteotomy (TPLO) plate and to compare its biomechanical properties with a locking compression plate (LCP) and plate-rod constructs for the stabilization of experimentally induced gap fractures in canine synthetic tibias. The tibial models were assigned to either repair with a modified TPLO plate (Group 1), locking compression plate construct (Group 2), or plate-rod construct (Group 3). The specimens were loaded to failure in axial compression, three-point mediolateral and craniocaudal bending. There was no statistical difference between the three groups regarding stiffness (N/mm) and deformation (mm) in axial compression. The modified TPLO plate achieved load to failure similar to the plate-rod construct in craniocaudal bending. There was no significant difference between groups on mediolateral bending tests regarding load to failure and deformation. Furthermore, there was no significant difference in stiffness between groups 1 and 2. In conclusion, the modified TPLO plate had similar mechanical properties to LCP and plate-rod construct in the axial compression and bending tests. Nonetheless, clinical studies with a large population of dogs are required to determine the value of this new implant in proximal tibial fracture repair.

Two Late Pleistocene human femora from Trinil, Indonesia: Implications for body size and behavior in Southeast Asia

Late Pleistocene hominin postcranial specimens from Southeast Asia are relatively rare. Here we describe and place into temporal and geographic context two partial femora from the site of Trinil, Indonesia, which are dated stratigraphically and via Uranium-series direct dating to ca. 37–32 ka. The specimens, designated Trinil 9 and 10, include most of the diaphysis, with Trinil 9 being much better preserved. Microcomputed tomography is used to determine cross-sectional diaphyseal properties, with an emphasis on midshaft anteroposterior to mediolateral bending rigidity (Ix/Iy), which has been shown to relate to both body shape and activity level in modern humans. The body mass of Trinil 9 is estimated from cortical area and reconstructed length using new equations based on a Pleistocene reference sample. Comparisons are carried out with a large sample of Pleistocene and Holocene East Asian, African, and European/West Asian femora. Our results show that Trinil 9 has a high Ix/Iy ratio, most consistent with a relatively narrow-bodied male from a mobile hunting-gathering population. It has an estimated body mass of 55.4 kg and a stature of 156 cm, which are small relative to Late Pleistocene males worldwide, but larger than the penecontemporaneous Deep Skull femur from Niah Cave, Malaysia, which is very likely female. This suggests the presence of small-bodied active hunter-gatherers in Southeast Asia during the later Late Pleistocene. Trinil 9 also contrasts strongly in morphology with earlier partial femora from Trinil dating to the late Early-early Middle Pleistocene (Femora II–V), and to a lesser extent with the well-known complete Femur I, most likely dating to the terminal Middle-early Late Pleistocene. Temporal changes in morphology among femoral specimens from Trinil parallel those observed in Homo throughout the Old World during the Pleistocene and document these differences within a single site.

The Role of the Intramandibular Joint, Symphyseal Tissues, and Wrapping Muscles on Theropod Dinosaur Mandibular Function

Sauropsids, unlike mammals, possess an intramandibular joint (IMJ) separating the dentary and postdentary bones, with different lineages either rigidifying (turtles & crocodilians) or maintaining compliance (lepidosaurs & birds) about the joint. IMJ construction and its role on mandibular performance is unclear, impeding our understanding of its function in extinct animals with extreme feeding behaviors like Tyrannosaurus rex. Sauropsids like T. rex pose a particular biomechanical paradox; feeding traces, coprolites, and their robust crania and teeth indicate that they regularly bit through and ingested bone, but their dorsoventrally-tall and mediolaterally-thin hemimandibles, IMJ, and patent mandibular symphyses suggest their mandibles were ill-suited for bone-crushing bites. Extant sauropsids also exhibit wrapping intramandibularis (mIRA) and pterygoideus ventralis (mPTv) muscles, whose effect on mandibular performance and interaction with the IMJ are unknown. Here we model the effect of the IMJ, symphyseal tissue properties, and wrapping muscle orientation on T. rex mandible biomechanical performance in order to give insight into the biomechanical constraints faced by sauropsids, and the link between their mandibular form and function. Joint tissue histology, gross dissection, and iodine contrast data of extant sauropsids were performed to inform model tissue properties, IMJ construction and muscle orientation. We imported a 3D STL model of the T. rex “STAN” and modeled the IMJ by differing material properties of modeled joint tissues to test the influence of linkage materials on mandibular performance. Muscles were mapped onto the model, and their corresponding muscle moments calculated from area centroids, muscle architecture, and volumes. Wrapping mIRA and mPTv muscles were simulated by directing their force vectors to appropriately positioned dummy points using extant sauropsids as a guide. The mandible was then constrained at the jaw joint and in a series of points along the tooth row from rostral to caudal, simulating both unilateral and bilateral bites. We find that while strains are quite high (>6000 µstrains) about the intramandibular joint, mediolateral bending stresses and strain are markedly reduced by the prearticular, which exhibits greater strain than the surrounding bone. Our results suggest the prearticular of T. rex acted as a strain sink to counteract bending about the IMJ and thereby rigidifying the mandible during feeding. We hypothesize that differences in IMJ articulation and prearticular construction may differentially facilitate or impede prearticular streptognathy, and thus intramandibular kinesis about the IMJ, in sauropsids. Forthcoming models will simulate other theropods within and without Tyrannosauridae to test this hypothesis.

A clinical study of functional outcome of unstable intertrochanteric fractures treated with proximal femur nail anti rotation system II

Introduction: Unstable, Comminuted intertrochanteric fractures continue to pose a challenge to the orthopaedic surgeon because of severe osteoporosis and medical disorders that increase the risks associated with surgery and anaesthesia. To overcome problems like lateral wall impingement and prominence of the proximal end of the nail, the PFNA-II was introduced in 2008. The PFNA II design modifications include the flat lateral shape of the proximal portion and a decrease in the mediolateral bending angle from 60 to 50. The purpose of the study is to confirm that the PFNA II eliminates the problem of lateral wall impingement experienced with previous intramedullary nailing systems and provides stable fracture fixation with positive functional outcome.Materials and methods: This study was conducted on 52 patient of which 32 were available till follow up of 6 months. All patients we were diagnosed with unstable intertrochanteric fracture of femur and underwent closed reduction internal fixation with proximal femoral nail anti-rotation system II. Functional outcome was assessed with Modified Haris Hip Score and radiological outcome was assessed with TAD score and implant related complications. Results: Fracture union was appreciated on an average 4 months of postoperative period. 4 patient had superficial infection while 1 patient had deep infection. Only 1 patient had cut off of helical blade. According to modified Harris hip score, excellent to good outcome was noted in more than 80% of patient. Conclusion: From our study, we conclude that PFNA II can be a reliable and implant of choice for the management of unstable intertrochanteric fracture with high rates of union, restoring the anatomical alignment with less chances of implant failure and reduced chance of implant failure.

Stiffness of a type II external skeletal fixator and locking compression plate in a fracture gap model.

To compare the stiffness of constructs fixed with a type II external skeletal fixator (ESF) or a 3.5-mm locking compression plate (LCP) in axial compression and bending with a fracture gap model. Quasi-static four-point bending and axial compression tests. Ten LCP and 10 ESF immobilizing epoxy cylinders with a 40-mm fracture gap. Five constructs of each type were tested in nondestructive mediolateral (ML) four-point bending and then rotated and tested in nondestructive craniocaudal (CC) four-point bending. Five additional constructs of each type were tested in nondestructive axial compression. Stiffness was compared between loading modes by construct type and between construct types by loading mode. Type II ESF were stiffer than LCP in ML bending (difference, 1474 N/mm, P < .0001) and in axial compression (difference, 458 N/mm, P = .008) but not in CC bending (P = .1673). Type II ESF were stiffer in ML bending than in CC bending (difference, 999 N/m, P < .0001), while LCP were stiffer in CC bending than in ML bending (difference, 634 N/mm, P < .0001). Type II ESF generated stiffer constructs compared with LCP in ML bending and in axial compression without a difference in CC bending. External skeletal fixator and LCP bending stiffness varied by loading direction. A type II ESF should be considered in a comminuted fracture requiring increased stability in ML and axial directions.

Development of a Novel Carbon-Fiber Mono-Lateral External Fixator

This paper reports the design of an innovative mono-lateral external fixator made of carbon fiber composite materials. The designed system can be easily assembled in comparison with commercial fixators and follows orthopedic requirements with sufficient stability and stiffness. The change of operation mode between distraction and fixation is achieved with a wedge that blocks axial translation in one position, while allows sliding with a 90º rotation. The prototypes were produced by the method of molding by compaction. A mold was developed for each part; the rail, the clamp and the cover. Each mold consisted of a cavity that gave form to the piece and a piston that exerted pressure on the composite. Mechanical tests were performed to determine the stiffness under axial compression, and anteroposterior and mediolateral bending. For comparison, tests were also performed on two Orthofix commercial systems, one with the rail made of carbon fiber and the other with an aluminum rail. The axial compression, anteroposterior and mediolateral bending stiffness of the developed system were 200.7, 13.4 and 87.0 N/mm, respectively, which were 38%, 35% and 27% lower than those obtained for the Orthofix system. However, these values were in the range of other similar systems reported in the literature. Therefore, the developed system presented promising results and may be clinically evaluated.

Evaluation of a Feline Bone Surrogate and In Vitro Mechanical Comparison of Small Interlocking Nail Systems in Mediolateral Bending.

The aim of this study was to (1) evaluate bending structural properties of a machined short fibre epoxy (SFE) feline bone surrogate (FBS), (2) compare the bending behaviour of small angle-stable interlocking nails (I-Loc; Targon) and locking compression plates (LCP) and (3) evaluate the effect of implant removal on FBS bending strength. Part 1: Feline cadaveric femurs (n = 10) and FBS (n = 4) underwent cyclic four-point bending and load to failure. Part 2: Fracture gap FBS constructs (n = 4/group) were stabilized in a bridging fashion with either I-Loc 3 and 4, Targon 2.5 and 3.0, LCP 2.0 and 2.4, then cyclically bent. Part 3: Intact FBS with pilot holes, simulating explantation, (n = 4/group) underwent destructive bending tests. Bending compliance, angular deformation and failure moment (FM) were statistically compared (p < 0.05). Native bone and FBS were similar for all outcome measures (p > 0.05). The smallest and largest bending compliance and angular deformation were seen in the I-Loc 4 and LCP 2.0 respectively (p < 0.05). While explanted Targon FBS had the lowest FM (p < 0.05), I-Loc and LCP constructs FM were not different (p > 0.05). The similar bending properties of short fibre epoxy made FBS and native feline femurs suggest that this model could be used for mechanical testing of implants designed for feline long bone osteosynthesis. The I-Loc constructs smaller angular deformation which also suggests that these implants represent a valid alternative to size-matched Targon and LCP for feline fracture osteosynthesis. The significantly lower FM of explanted Targon may increase the risk of secondary fracture following implant removal.

Biomechanical evaluation of retrograde docking nailing to a total hip arthroplasty stem in a periprosthetic femur fracture model

IntroductionSlotted nails allow a connection to a total hip arthroplasty (THA) stem and act as intramedullary load carrier. This study compares construct stiffness, cycles to failure and failure load between a retrograde slotted femur nail construct docked to a THA stem and a lateral locking plate in a human periprosthetic femur fracture model. Materials and methodsIn seven pairs of fresh-frozen human anatomic femora with cemented THA, a transverse osteotomy was set simulating a Vancouver type B1 fracture. The femora were instrumented pairwise with either a retrograde slotted nail coupled to the prosthesis stem, or a locking plate plus a locking attachment plate. Four-point mediolateral bending, torsional and axial bending construct stiffness was investigated via non-destructive tests. Cyclic testing under progressively increasing physiologic loading was performed at 2 Hz until catastrophic construct failure. ResultsMediolateral bending stiffness did not differ significantly between the two groups (P=0.17) but exhibited a biphasic profile with significantly increased stiffness in both groups (P<0.01). Nail constructs provided a significantly lower torsional stiffness (0.49 ± 0.66 Nm/°) than plate constructs (1.70 ± 0.86 Nm/°), P=0.03. Axial bending stiffness did not differ significantly between the groups (Nail: 605 ± 511 N/mm; Plate: 381 ± 428 N/mm), P=0.61. Cycles to failure and failure load were significantly higher for the plate constructs (25’700 ± 8’341; 3’070 ± 1334 N) compared with the nail constructs (20’729 ± 7’949; 2’573 ± 1295 N), P=0.04. ConclusionThe docking nail construct provides an intramedullary fixation with connection to the prosthesis stem; however, it is biomechanically weaker in stable fractures compared to the plate construct.

Locomotion on the edge: Structural properties of the third metacarpal in Thoroughbred and Quarter Horse racehorses and feral Assateague Island ponies.

The elongated, distally tapered limbs of horses are adapted for high-speed locomotion. Because these traits are artificially selected for in modern racehorses, they operate at a morphological extreme with a high risk of fracture. Racehorses are subject to different training and racing regimes depending on their breed and gait, and are therefore an interesting model to examine bone functional adaptation under variable biomechanically intense conditions. This study compares bone structural properties in the third metacarpal (MCIII) of Thoroughbred (n = 9) and Quarter Horse (n = 11) racehorses, using feral Assateague Island ponies (n = 6) as an untrained/unraced outgroup, to determine whether structural properties reflect variable racing and training regimes. Geometric section properties and bone mineral densities were determined using peripheral quantitative CT at two diaphyseal sites and through the distal epiphysis. Diaphyseal strength of the MCIII in all three breeds does not differ relative to body size, but in the mid-diaphyseal region Thoroughbreds have higher antero-posterior relative to medio-lateral bending strength than Quarter Horses, as well as higher bone mineral densities in left MCIII epiphyses (particularly in the lateral condyle). Interestingly, all breeds have lower bone mineral densities in the lateral versus medial condyle, an inherent structural feature that may influence predisposition to fracture when running around turns. Our results suggest that despite subtle differences in bone structure between different racehorse breeds, basic morphology of the third metacarpus is relatively similar among racing and non-racing horses, possibly reflecting intense selection (natural and artificial) across domestic equids for similar structural features within distal limb elements.

Biomechanical study of a low-cost external fixator for diaphyseal fractures of long bones

BackgroundExternal fixation improves open fracture management in emerging countries. However, sophisticated models are often expensive and unavailable. We assessed the biomechanical properties of a low-cost external fixation system in comparison with the Hoffmann® 3 system, as a reference.MethodsTransversal, oblique, and comminuted fractures were created in the diaphysis of tibia sawbones. Six external fixators were tested in three modes of loading—axial compression, medio-lateral (ML) bending, and torsion—in order to determine construction stiffness. The fixator construct implies two uniplanar (UUEF1, UUEF2) depending the pin-rods fixation system and two biplanar (UBEF1, UBEF2) designs based on different bar to bar connections. The designed low-cost fixators were compared to a Hoffmann® 3 fixator single rod (H3-SR) and double rod (H3-DR). Twenty-seven constructs were stabilized with UUEF1, UUEF2, and H3-SR (nine constructs each). Nine constructs were stabilized with UBEF1, UBEF2, and H3-DR (three constructs each).ResultsUUEF2 was significantly stiffer than H3-SR (p < 0.001) in axial compression for oblique fractures and UUEF1 was significantly stiffer than H3-SR (p = 0.009) in ML bending for transversal fractures. Both UUEFs were significantly stiffer than H3-SR in axial compression and torsion (p < 0.05), and inferior to H3-SR in ML bending, for comminuted fractures. In the same fracture pattern, UBEFs were significantly stiffer than H3-DR (p = 0.001) in axial compression and torsion, while only UBEF1 was significantly stiffer than H3-DR in ML bending (p = 0.013).ConclusionsThe results demonstrated that the stiffness of the UUEF and UBEF device compares to the reference fixator and may be helpful in maintaining fracture reduction. Fatigue testing and clinical assessment must be conducted to ensure that the objective of bone healing is achievable with such low-cost devices.

Biomechanical Comparison of Two Locking Plate Constructs for the Stabilization of Feline Tibial Fractures.

The aim of this study was to compare the biomechanical characteristics of locking compression plate (LCP) and conical coupling plate (CCP) constructs for the stabilization of experimentally induced gap fractures in cat tibiae. Pelvic limbs were harvested from eight cat cadavers. Paired tibiae were stripped of all soft tissues, and randomly assigned to the LCP or CCP stabilization group. An eight-hole 2.7 mm LCP or a six-hole 2.5 mm CCP was applied to the medial surface of each tibia. A 1-cm segment of the tibia was excised centrally beneath the plate. The specimens were potted, then tested in non-destructive four-point craniocaudal and mediolateral bending, followed by non-destructive axial compression. Each construct was subsequently loaded to failure in axial compression. Bending and axial stiffness, yield load and failure load were calculated for each specimen. The LCP constructs were significantly stiffer than the CCP constructs when subjected to non-destructive bending and axial loading. Craniocaudal bending stiffness was significantly greater than mediolateral bending stiffness for both constructs. Yield load and failure load were significantly greater for LCP constructs compared with CCP constructs. LCP may be a more suitable implant for stabilizing complex diaphyseal tibial fractures in cats. Additional supplemental fixation should be considered when using CCP to stabilize unreconstructed diaphyseal tibial fractures in cats. Further clinical investigation of both implants is recommended.

Development of an easy-to-assemble external fixation system for bone transport

We developed a monolateral external fixation system for bone elongation easier to assemble than commercial systems since the clamps can be laterally assembled to the rail in contrast to the commercial systems where the clamps can only be assembled from the ends. The system is formed by clamps, screws, and a rail. The rail is composed of two carbon fiber circular rods of ½” diameter and 42-cm length. The clamps can slide with respect to the rail to allow bone distraction or may be fixed to the rail by using a wedge and a screw. The prototype was mechanically evaluated under the ASTM F1541 standard. The stiffness in axial, Medio Lateral bending (ML), Antero Posterior bending (AP), and flexo-torsional load were 117 N/mm, 46 N/mm, 8.7 N/mm, and 5.8 N-mm/°, respectively, which are on the range of stiffness of commercial systems.

New Specimens of the Late Cretaceous Metatherian Eodelphis and the Evolution of Hard-Object Feeding in the Stagodontidae

The Stagodontidae include the largest metatherians known from the Cretaceous of North America. Of the recognized species of the stagodontid genus Eodelphis, E. cutleri is larger and has a more robust dentary, more inflated premolars, and third premolars specialized for crushing, as opposed to the more gracile E. browni. These differences have led to the hypothesis that an E. cutleri-like ancestor gave rise to Didelphodon—another, mostly younger, stagodontid, which has been interpreted as a durophagous predator-scavenger. If correct, E. cutleri would be expected to show more morphological adaptation toward durophagy than E. browni does. Here, we describe two new dentary fossils referable to E. browni and test the evolutionary hypothesis by applying beam theory to estimate bending force capabilities of 22 dentaries of Cretaceous stagodontids and other metatherians. The resulting diversity of bending force profiles of the sampled dentaries implies that Cretaceous metatherians had a wide range of feeding behaviors. Among the stagodontids, E. cutleri has a mediolateral bending force profile of the dentary that is more similar to that of Didelphodon than it is to that of E. browni; whereas its dorsoventral bending force profile is more similar to that of E. browni. These results indicate that anteriorly the dentary of E. cutleri was capable of resisting high torsional stresses from hard-object feeding but lacked other dorsoventral buttressing associated with exceptionally high bite forces of Didelphodon. Our results imply that some morphological changes associated with durophagy evolved twice within this clade, independently in E. cutleri and Didelphodon.

Long bone diaphyseal shape follows different ontogenetic trajectories in captive and wild gorillas.

A number of studies have demonstrated the ontogenetic plasticity of long bone diaphyseal structure in response to mechanical loading. Captivity should affect mechanical loading of the limbs, but whether captive apes grow differently than wild apes has been debated. Here, we compare captive and wild juvenile and adult Gorilla to ascertain whether growth trajectories in cross-sectional diaphyseal shape are similar in the two environments. A sample of young juvenile (n = 4) and adult (n = 10) captive Gorilla gorilla gorilla specimens, with known life histories, were compared with age-matched wild G.g. gorilla (n = 62) and G. beringei beringei (n = 75) in relative anteroposterior to mediolateral bending strength of the femur, tibia, and humerus. Cross sections were obtained using peripheral quantitative CT. Captive and wild adult G.g. gorilla differed in bending strength ratios for all three bones, but these differences were not present in young juvenile G.g. gorilla. In comparisons across taxa, captive juvenile G.g. gorilla were more similar to wild G.g. gorilla than to G.b. beringei, while captive adult G.g. gorilla were more similar in shape to G.b. beringei in the hind limb. Captive and wild G. gorilla follow different ontogenetic trajectories in long bone diaphyseal shape, corresponding to environmental differences and subsequent modified locomotor behaviors. Differences related to phylogeny are most evident early in development.

Bilateral asymmetry of the humerus in Neandertals, Australian aborigines and medieval humans.

Bilateral asymmetry of diaphyseal shape and size may be a reflection of relative activity levels and patterns of habitual biomechanical stress in the upper arms of Neandertals and Homo sapiens. The main purpose of our study was to assess the level of directional asymmetry of humeral cross sections in Neandertals, recent Australian aborigines, and medieval farmers. Indices of directional and absolute asymmetry (%DA and %AA) of humeral cross-sectional properties in Neandertals and recent Homo sapiens were calculated. Evenly distributed semilandmarks around the external and internal borders of cortical bone were digitized in the course of computed tomography for analysis of shape differences between sides of the body. The medieval farmers were characterized by significant %DA and %AA for polar second moment of area (J), ratio of maximum to minimum second moments of area, and ratio of antero-posterior to medio-lateral bending strength. In Australian aborigines, only J in males shows significant %DA and %AA, while Neandertals exhibit no significant asymmetry of any cross-sectional properties. Differences in cross-sectional shape between sides of the body were established in all three analyzed groups. High levels of directional asymmetry of cross-sectional shape and properties in medieval farmers may be caused by the performance of more physically demanding tasks using one side of the body from an early age in that population. Various patterns of asymmetry in Neandertals and modern humans may be caused by different habitual behaviors during growth, eco-geographic patterns in body proportions, genetic factors, and differences in ontogeny.

Geometric Properties of the Third Metacarpal Bone: A Comparison Between Thoroughbred and Quarter Horse Racehorses

A frequent reason that Thoroughbred racehorses are killed during racing or training is fracture of the third metacarpal (the cannon bone). Determining the factors involved in these catastrophic cannon bone fractures is imperative to improving both horse and jockey safety. Though seemingly spontaneous, catastrophic cannon bone fractures generally result from the combination of mechanical forces commonly present in racing Thoroughbreds, and generally materialize in the distal lateral condyle of the cannon bone. Thoroughbreds, however, are not the only breed of racehorse to experience catastrophic fractures—Quarter Horses also exhibit specific patterns of injury in more proximal limb bones. Different fracture patterns in these two breeds of galloping racehorse may indicate different loading patterns of their limbs, which could be a product of varied training programs, or variance in breed morphology. The present analysis compares geometric properties of the third metacarpal in Thoroughbred and Quarter Horse racehorses, using feral Assateague Island ponies as an outgroup, in order to clarify the reasons for distinctive fracture patterns in different breeds of racehorses.Six cannon bones from each breed were obtained from New Bolton Center (Thoroughbreds), Oklahoma State University (Quarter Horses), and the Assateague Island National Seashore (Assateague Island ponies). Peripheral Quantitative Computed Tomography (pQCT) generated cross sections at 40% and 20% (distal) of bone length, and at the midpoint of the distal articular surface. Cross‐sectional areas, second moments of area, and section moduli were calculated for the two diaphyseal sections, and trabecular bone mineral density and total bone mineral density were determined for the distal articular section. Approximate body masses were estimated from proximal articular breadths using a published formula.Analysis of covariance (ANCOVA) revealed that although the overall strength of cannon bones in Thoroughbreds and Quarter Horses did not differ relative to body size, Thoroughbreds had relatively higher anteroposterior bending strength in the diaphysis than Quarter Horses, and relatively lower mediolateral bending strength. Thoroughbreds also had lower values of trabecular and total bone densities in the distal articular section, which is the region where lateral condyle fractures are generally incurred by Thoroughbreds but not Quarter Horses. Both Thoroughbreds and Quarter Horses had lower overall safety factors compared to feral Assateague Island ponies. Assateague Island ponies exhibited relatively stronger bone shafts and a higher trabecular density in the distal articular section. Results suggest that breed‐specific bone structure differences contribute to the greater incidence of fractures in domestic racehorses and, relatedly, the common location of fractures in Thoroughbreds.Support or Funding InformationFunded by the American Society of Mammalogists.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Biomechanical Evaluation of Glenoid Reconstruction With an Implant-Free J-Bone Graft for Anterior Glenoid Bone Loss

Background: The anatomic restoration of glenoid morphology with an implant-free J-shaped iliac crest bone graft offers an alternative to currently widely used glenoid reconstruction techniques. No biomechanical data on the J-bone grafting technique are currently available. Purpose: To evaluate (1) glenohumeral contact patterns, (2) graft fixation under cyclic loading, and (3) the initial stabilizing effect of anatomic glenoid reconstruction with the implant-free J-bone grafting technique. Study Design: Controlled laboratory study. Methods: Eight fresh-frozen cadaveric shoulders and J-shaped iliac crest bone grafts were used for this study. J-bone grafts were harvested, prepared, and implanted according to a previously described, clinically used technique. Glenohumeral contact patterns were measured using dynamic pressure-sensitive sensors under a compressive load of 440 N with the humerus in (a) 30° of abduction, (b) 30° of abduction and 60° of external rotation, (c) 60° of abduction, and (d) 60° of abduction and 60° of external rotation. Using a custom shoulder-testing system allowing positioning with 6 degrees of freedom, a compressive load of 50 N was applied, and the peak force needed to translate the humeral head 10 mm anteriorly at a rate of 2.0 mm/s was recorded. All tests were performed (1) for the intact glenoid, (2) after the creation of a 30% anterior osseous glenoid defect parallel to the longitudinal axis of the glenoid, and (3) after anatomic glenoid reconstruction with an implant-free J-bone graft. Furthermore, after glenoid reconstruction, each specimen was translated anteriorly for 5 mm at a rate of 4.0 mm/s for a total of 3000 cycles while logging graft protrusion and mediolateral bending motions. Graft micromovements were recorded using 2 high-resolution, linear differential variable reluctance transducer strain gauges placed in line with the long leg of the graft and the mediolateral direction, respectively. Results: The creation of a 30% glenoid defect significantly decreased glenohumeral contact areas (P < .05) but significantly increased contact pressures at all abduction and rotation positions (P < .05). Glenoid reconstruction restored the contact area and contact pressure back to levels of the native glenohumeral joint in all tested positions. The mean (±SD) force to translate the humeral head anteriorly for 10 mm (60° of abduction: 31.7 ± 12.6 N; 60° of abduction and 60° of external rotation: 28.6 ± 7.6 N) was significantly reduced after the creation of a 30% anterior bone glenoid defect (60° of abduction: 12.2 ± 6.8 N; 60° of abduction and 60° of external rotation: 11.4 ± 5.4 N; P < .001). After glenoid reconstruction with a J-bone graft, the mean peak translational force significantly increased (60° of abduction: 85.0 ± 8.2 N; 60° of abduction and 60° of external rotation: 73.6 ± 4.5 N; P < .001) compared with the defect state and baseline. The mean total graft protrusion under cyclical translation of the humeral head over 3000 cycles was 138.3 ± 169.8 µm, whereas the mean maximal mediolateral graft deflection was 320.1 ± 475.7 µm. Conclusion: Implant-free anatomic glenoid reconstruction with the J-bone grafting technique restored near-native glenohumeral contact areas and pressures, provided secure initial graft fixation, and demonstrated excellent osseous glenohumeral stability at time zero. Clinical Relevance: The implant-free J-bone graft is a viable alternative to commonly used glenoid reconstruction techniques, providing excellent graft fixation and glenohumeral stability immediately postoperatively. The normalization of glenohumeral contact patterns after reconstruction could potentially avoid the progression of dislocation arthropathy.