Abstract Objective: This study was to characterize individual path trajectories during the 50-step Fukuda stepping test (FST) and to determine the impact of step height on these trajectories. Methods: Kinematic data from the shoulders and feet were recorded in 12 young healthy adults while performing the FST at a comfortable step height (CoStep) and while stepping with lifting the knees high (HiStep). Path trajectories were depicted by step-by-step displacement of the right and left toes, and body rotation was obtained from the rotation of the shoulders. The effect of step height on the distance traveled and the final anteroposterior (AP) and mediolateral displacements and rotation were determined with paired t-tests. Results: Path trajectories were composed of various combinations of forward displacement, lateral deviation, and rotation to the left or right. In comparison with CoStep, the mean AP displacement and mean distance traveled were significantly shorter in HiStep (P < 0.05) while the mean rotation was significantly larger in HiStep (P < 0.05), but the displacement patterns were not modified. Conclusion: We found that the path trajectories vary greatly among participants, and that step height has a significant impact on the magnitude of displacements and rotation during the FST.