Medieval Bologna was a vibrant and dynamic city with hundreds of artisans, craftsmen, students, government officials (and other professionals), as well as animals, all sharing the same streets, neighborhoods, and waterways. As this paper will show, medieval officials were highly concerned with keeping their city and its environs, safe and healthy, and devoted significant amounts of time and resources to achieve these goals. Using Bologna as a case study, this article maps in GIS over one hundred regulations from the city’s urban statutes from 1245-67, to examine public health strategies. In doing so, it will demonstrate that medieval officials in Bologna employed a spatial logic as they created health regulations and oversaw the construction and maintenance of infrastructure. This spatial logic was grounded in an understanding of movement and an awareness of how, where, and when people, animals, goods, water, and waste moved through the city. In this way, the knowledge of space and movement were critical aspects of public health strategies which officials deployed to maintain a healthy and working city.