Purpose: The study of "the influence of substrate on growth and development of Ganaderma lucidum cultivation in Bao Loc - Lam Dong" was conducted at the Technology College of economy and technology of Bao Loc from May to September 2023 with the aims of selectingsuitable substrates for growth and development in Reishi, completed the processes of Reishi artificial cultivation in Bao loc. Methodology: Database involved nine treatments: rubber tree sawdust 75% + rice bran 25% supplemented urea 0,25%; rubber tree sawdust 95% + rice bran 5% supplemente urea 0,25% and DAP 0,25%; rubber tree sawdust 100% supplemented SA 0,5% and DAP 0,25%; rubber tree sawdust 96% + 2% maize powder + 2% rice bran; rubber tree sawdust 90% + maize powder 10% ; rubber tree sawdust 75% + maize powder 10% + rice bran 15%; rubber tree sawdust 95% + rice bran 5% supplemented DAP 0,2% , SA 0,2% and urea 0,1%; rubber tree sawdust 70% + rice bran 30%; control treament (rubber tree sawdust 90% + rice bran 5% + 5% maize powder). Experimental design was a Completely Randomized Design with 3 replications. The data were analyzed by using the analysis of variance (ANOVA) and group means were compared by Duncan Multiple Range Test (DMR) using the SAS 9.1 program. Means separation were computed following Duncan’s Multiple Range Test (DMRT). Findings: Results were achieved: Significant difference was observed in biological yield, dry yield and biological efficiency of Reishi (Ganaderma lucidum) on differences substracts .The highest biological yield (52,11 g/packet) and biological efficiency (15,79%) was obtained from mixed rubber sawdust 70% + 30% ridce brain Unique Contribution toheory, Policy and Practice: Complete the process of growing Ganoderma mushrooms in Bao Loc, orienting the development of Lam Dong into a large medicinal mushroom production area in Vietnam, creating Ganoderma mushroom products for export to the world.