Herbal Medicinal Products Research Articles

Herbal medicinal therapy for haemorrhoid and sexual dysfunction - perception and practice among adult males in Ibadan metropolis, Southwest Nigeria

The application of herbal medicinal products for diverse therapeutic purposes is on the rise inSouthwestern, Nigeria due to its relative effectiveness, accessibility, and affordability. This study aimed to assess the perception and application of herbal medicinal products for the treatment of haemorrhoid and sexual dysfunction (SD) among adult males in Ibadan metropolis, Southwest Nigeria. The study involved 283 randomly selected adult male residents of the Ibadan metropolis, between 20 to 69 years old, and covering different marital statuses, levels of education and professional careers. A questionnaire-based survey was conducted to evaluate the perception and experience of the subjects about the application of herbal-based therapy for haemorrhoid and SD. Based on the results, the most dominant groups include 20 to 29 years old (51.6%), single (59.4%), first-degree holders (55.8%), and public servants (52.7%). Furthermore, 61.5% of the respondents reported prior experience of haemorrhoid; 73.8% opined that the condition could lead to SD; 87% and 60.2% believed that herbal medicine can be used to treat haemorrhoid and SD respectively. There was correlation between the age and level of education of respondents with methods of treating SD and experience of side effects due to herbal therapy. The study population holds an affirmative opinion that herbal medicinal products are effective for the treatment of haemorrhoids and SD. The application of herbal therapy will continue to increase among the study population hence, there is a need to regulate and standardize its production and therapeutic applications.

Content of Bioactive Compounds in Highbush Blueberry Vaccinium corymbosum L. Leaves as a Potential Raw Material for Food Technology or Pharmaceutical Industry.

This study was performed to investigate the content of selected phenolic compounds, antioxidant activity and the levels of arbutin and hydroquinone in 25 varieties of highbush blueberry (Vaccinium corymbosum) leaf samples. An analysis of the bioactive components was performed using the HPLC technique and the antioxidant activity was determined via spectrophotometric methods. The content of chlorogenic acid in the analysed leaf extracts ranged from 52.76 mg/g (Spartan variety) to 32.37 mg/g (Nelson variety) and was present in the highest concentration among all the analysed phenolic acids. Particularly large levels of isoquercetin were found in the Aurora, Ivanhoe and Toro varieties (28.40 mg/g, 26.24 mg/g and 21.57 mg/g, respectively). An exceptionally high rutin content (p < 0.05) was found in the Ivanhoe variety (27.19 mg/g) as compared to the other varieties, where it ranged from 2.06 mg/g (Earliblue and Patriot varieties) to 10.55 mg/g (Bluejay variety). The Patriot variety was determined to possess the highest antioxidative activity using the FRAP method (1086.15 μmol Trolox/g d.w.) and based on its DPPH radical scavenging activity (1124.17 μmol Trolox/g d.w.). The total phenolic content (TPC) determined via spectrophotometry ranged from 48.11 mg GAE/g d.w. (Elizabeth variety) to 177.31 GAE/g d.w. (Patriot variety). The arbutin content in the leaves of all tested varieties exceeded 2%, so it can be concluded that they constitute a stable source of arbutin. Three varieties (Bonus, Chanticleer and Herbert) can be considered a potential alternative to bearberry and lingonberry leaves. The hydroquinone content in the analysed extracts was determined to be at a lower level. V. corymbosum leaves can be considered an interesting herbal material for use in traditional herbal medicinal products but not directly for food products and dietary supplements.

Microbial and heavy metal contamination in herbal medicine: a prospective study in the central region of Saudi Arabia

IntroductionHerbal medicine is a medical system based on the utilization of plants or plant extracts for therapy. The continual increase in global consumption and the trade of herbal medicine has raised safety concerns in many regions. These concerns are mainly linked to microbial contamination, which could spread infections with multi-resistant bacteria in the community, and heavy metal contamination that may lead to cancers or internal organs’ toxicity.MethodsThis study was performed using an experimental design. A total of 47 samples, herbal medicine products sold in local stores in Qassim region, were used in the experiments. They were tested for bacterial contamination, alongside 32 samples for heavy metal analysis. Bacterial contamination was determined by the streak plate method and further processed to determine their antimicrobial susceptibility patterns using MicroScan WalkAway96 pulse; heavy metals were determined using a spectrometer instrument.ResultsA total of 58 microorganisms were isolated. All samples were found to be contaminated with at least one organism except three samples. The majority of the isolated bacterial species were gram negative bacteria, such as Klebsiella spp., Pseudomonas spp. and E. coli., which could be of fecal origin and may lead to pneumonia, skin, or internal infections. Furthermore, most of the gram-positive bacteria were found to be multi-drug resistant. Moreover, for heavy metals, all samples had levels exceeding the regulatory limits.ConclusionThis study demonstrated the presence of bacteria and heavy metals in samples of herbal medicines. Using these contaminated products may spread resistant infections, metal toxicities, or even cancers in the community.

Ethical considerations in the regulation and use of herbal medicines in the European Union.

The regulation and use of herbal medicines is a topic of debate due to concerns about their quality, safety, and efficacy. EU Directive 2004/24/EC on Herbal Medicinal Products was a significant step towards establishing a regulatory framework for herbal medicinal products in the EU, and bridging the gap between conventional and herbal medicines. This Directive allows herbal medicinal products to be marketed in the EU through full marketing authorisation, well-established use, and traditional use of herbal medicinal products. The framework relies on the correlation between the therapeutic claims of herbal medicine and the scientific evidence backing them up: the greater the claims made regarding medicinal benefits, the more evidence is required to substantiate its efficacy and safety. This regulatory framework acknowledges and incorporates traditional knowledge when evaluating herbal medicines, showcasing a balanced approach that values cultural traditions while mandating monographs for traditional herbal medicinal products. Excluding herbal medicines completely limits access to affordable treatment, particularly when they serve as the only alternative for some, and protects consumer autonomy. This EU framework could therefore serve as a practical guidance for the use and regulation of herbal medicines, even outside the EU. In conclusion, it is argued that the same moral imagination and courage shown by regulators in the case of herbal medicines could perhaps be used in the regulatory frameworks of other healthcare products.

Interest Groups and COVID-19 in Africa(2019-2022).

The study highlights the crucial roles played by interest groups in shaping the definition and redefinition of policy issues related to the COVID-19 pandemic in Africa. The study focused on selected countries to discuss strategies that were deployed to combat the COVID quandary. The selected countries are Nigeria, Ghana, Kenya, Tanzania, South Africa, Zimbabwe, Egypt, and Tunisia. A purely qualitative research, it relied on existing literature sources to produce thematic analysis which explore the various strategies adopted by interest groups in addressing the COVID-19 pandemic in Africa. The study revealed the challenges posed by the COVID-19 pandemic to the already fragile healthcare system in Africa. Again, it revealed the innovative approaches such as herbal medicine and local production of ventilators developed by African countries to tackle COVID-19 including efforts of other interests groups like government agencies, private institutions and international organizations in the fight against COVID-19 in Africa.

Drying kinetics and quality attributes analysis of moringa (Moringa oleifera) leaves under distinct drying methods

AbstractThe present work aimed to analyze the drying kinetics and color degradation of moringa leaves in selected drying methods. Also, quantitatively analyze the retention of phytochemicals and antioxidant capacity. Moringa leaves were dried using three methods, namely, freeze‐drying (−42 ± 2°C, 0.024 ± 0.001 mbar), microwave drying (200, 400, and 600 W), and hot air oven drying (55, 65, and 75°C). Drying kinetics revealed that the drying of moringa leaves occurred in the falling rate period, which means the diffusion process controlled the moisture removal from leaves. The color degradation was most minor in freeze‐dried leaves, followed by microwave‐dried leaves at 200 W. The value of total phenolic content was found maximum (57.42 ± 2.49 mg GAE/g dm) under microwave drying at 200 W, while the same was found least in quantity (24.62 ± 1.10 mg GAE/g dm) for samples dried under hot air oven drying at 65°C. A similar pattern was observed for total flavonoid content. The antioxidant capacity of the samples dried in the freeze dryer was highest at 5.94 ± 0.25 mg GAE/g dm. This research revealed that microwave drying at 200 W is best regarding the overall quality attributes of moringa leaves.Practical ApplicationsDrying is one of the essential processes in the food industry, for example, drying is crucial for the production of herbal medicines, nutritional supplements and extracts, leaf‐based culinary ingredients, aroma and essential oils, natural dyes and pigments, etc. It is used to increase the shelf‐life of agricultural produce; it facilitates the transportation and storage of food products. This paper may help in the selection of the drying method and condition for leaves drying to have desired characteristics in the final product like color, bioactive compounds, or antioxidant properties; May help in the trade‐off among quality, time, and cost of the production. Moreover, it may be helpful in the designing of the multi‐mode dryer which may produce superior quality products with great energy efficiency in less time. Which further facilitates the manufacturing of nutritionally rich food products.

Effect of Cymbopogon olivieri-based herbal vaginal product on bacterial vaginosis.

Bacterial vaginosis is the most common vaginal infection in reproductive-age women. If it is not treated, the quality of life will be reduced. In this study, the herbal medicine product Cymbopogon olivieri was used for its treatment. This study was conducted with 90 women. The patients were randomly divided into two groups of 45: Cymbopogon olivieri and metronidazole. The treatment period was 7 days for each group. Improvement status was determined by eliminating at least three out of four of Amsel's criteria. A new variable with two order levels (negative and positive) was constructed. This new variable shows the status of the treatment process. Chi-square and Fisher's exact tests were used to examine the relationship between the new variable and treatment status. The results demonstrate that Cymbopogon olivieri and metronidazole significantly reduced the burning, itching, malodor, abnormal vaginal discharge, pH, clue cell, and positive whiff test (p<0.05). The findings also demonstrate that neither treatment was statistically different from the other for at least three of Amsel's criteria. This study shows that the effect of Cymbopogon olivieri on bacterial vaginosis is similar to that of metronidazole. Hence, Cymbopogon olivieri is a suitable option to treat bacterial vaginosis.

Identification of Orthosiphon stamineus from different phytogeographical zones in Indonesia by FTIR-fingerprinting and chemometrics

Orthosiphon stamineus (OS) or cat’s whiskers (Lamiaceae) is one of the herbs broadly used as raw materials for functional food and traditional remedies, for it contains phenolic acids and flavonoids. OS grows well in tropical and subtropical regions of Asia and Africa. Phytogeographical characteristics are among many variables that greatly control plant growth and phytochemical content. This research was conducted to analyze and classify 14 samples of OS leaves from different regions in Indonesia according to phytogeographic profiles using a combination of Fourier-transform infrared (FTIR) fingerprinting and chemometrics, i.e., principal component analysis (PCA) and cluster analysis (CA). The first two PCs were used to show the grouping of 14 samples with a cumulative variance at 91%. Results showed that all samples had comparable FTIR spectra showing the stretching frequencies of O-H, C-H, and C=C bonds. Further discrimination with PCA and CA was able to classify them into four groups of different phytogeographical origins: group 1 comprised OS samples from Tawangmangu, Mojokerto, Batu, Sampang, Madiun, South Jakarta, Jombang, Surabaya, Kediri, and Ngawi, group 2 from Lampung and Gresik, group 3 from Badung, and group 4 from Kotabaru. Thus, it can be inferred that FTIR fingerprinting combined with chemometrics can distinguish between OS crude drugs from different locations. Introducing a wider variety of OS analytical methods is believed to positively influence the flexibility and ease of quality control of herbal ingredients, ultimately ensuring the safety and efficacy of herbal medicinal products.

Architectural Framework for Development of Fuzzy Integrated Data Repository (FIDR) for Medicinal Plants in Nigeria.

Nigeria is a tropical country known to be home to a vast array of plants, most of which are plants that have been found to be medicinal and have been used in traditional healthcare delivery for centuries. Traditional Medicine Practitioners (TMPs) have been the major custodians of the knowledge of herbal medicine in Nigeria. It is noted that there is lack of documentation of the practice of herbal medicine which has resulted in the loss of a vast majority of the knowledge. The absence of a centralized digital data repository on medicinal plants and their uses in Nigeria hampers the drive for standardization, scientific validation and quality control of herbal medicine products in Nigeria. This consequently undermines the health and wellbeing of those dependent on traditional herbal medicine for their primary health care needs. Much of the claims on the efficacy of herbal medicine are found to be based on traditional beliefs and myth and have not been subjected to scientific evaluation. This accounts for the introduction of fuzzy logic into the design of an architectural framework for design of Fuzzy Integrated Data Repository that will allow access to information using sharp and fuzzy queries. This will be a resource platform that will resolve the information impasse by providing adequate and correct information to all stakeholders in the traditional herbal medicine healthcare delivery as well as foster synergy between orthodox medicine and herbal medicine practitioners, improve research that will lead to standardization and quality control of herbal medicine products in Nigeria.

Herbal Tourism Revitalization Efforts after Pandemic

Abstract. Herbal medicine is a mainstay of rural areas, especially around the Menoreh mountains, Kulonprogo. ‘Rumah Jamu Menoreh’ is a tourist attraction and herbal medicine production place that was established in 2015 in Kemiriombo, Gerbosari, Samigaluh, Kulon Progo. For approximately 7 years, Rumah Jamu has produced a variety of products, including wedang uwuh, instant turmeric acid, ginger syrup, coconut oil, coconut vinegar, secang syrup, ganyong flour, and instant red ginger. During the last pandemic, this herbal house was also affected. For about 2 years it experienced sluggishness, decreased turnover and no tourist visits. This condition made ‘Rumah Jamu Menoreh’ even worse. The owner of the herbal house feels that he has not been able to rise up in the face of these rapid changes. Problems that can be identified include: 1) Lack of understanding of the pandemic that has an impact on tourism, 2) Lack of ability of partners to create new products as a form of innovation, and 3) Online marketing already exists but has not been fully implemented. From the problems that were identified, this community service program carried out various activities to solve these problems. The activities that have been carried out include 1) training on the management of diversification and variation of herbal products, 2) assisting the procurement of equipment to support quality products, 3) optimizing digital-based promotional media and social media, and 4) providing management assistance to be able to market products more expansively and create a wider marketing network. The result of this community service program is that partners have understood that the pandemic has a serious impact on tourism, especially herbal tourism. Partners are able to diversify in the form of other small foods and are able to market using digital devices. In addition, with management assistance, it can rise again to sell its products.

Study of the Elemental Composition of Pectoral Species No. 2 and Its Components

INTRODUCTION. The influence of anthropogenic factors on the content of toxic elements in plants increases the need for monitoring the content of toxic elements in medicinal plant raw materials and the corresponding herbal medicinal products. In addition, studying the elemental composition of herbal medicinal products will help determine their potential in the treatment of elemental metabolism disorders (macroand micro-elementoses).AIM. This study aimed to determine the elemental composition and the heavy metal content in Pectoral Species No. 2 and its components.MATERIALS AND METHODS. The study used Pectoral Species No. 2 and its components, including common coltsfoot (Tussilago farfara L.) leaf, greater plantain (Plantago major L.) leaf, and liquorice (Glycyrrhiza glabra L., G. uralensis Fisch.) root. The samples were manufactured by Krasnogorskleksredstva JSC and purchased at Moscow pharmacies. To determine the elemental composition, the study samples were digested with concentrated nitric acid and analysed by inductively coupled plasma mass spectrometry (ICP-MS). The calibration involved using concentrations ranging from 0.5 to 1.5 times the maximum permissible concentration for each element.RESULTS. This study identified and quantified 26 elements in Pectoral Species No. 2 and its components. The content of heavy metals was within the permissible concentrations. The study established the predominant macronutrients (potassium, calcium, and magnesium), micronutrients (iron, aluminium, and strontium), and ultramicronutrients (barium, nickel, and chromium) in Pectoral Species No. 2. The authors evaluated the contributions of each herbal drug to the total element content in the medicinal product.CONCLUSIONS. Coltsfoot leaf exerts the greatest influence on the content of macro-, micro-, and ultramicronutrients in Pectoral Species No. 2. Greater plantain leaf contributes the most to the zinc, silver, and nickel content. Liquorice root has the highest strontium and molybdenum content.

Stakeholders’ Attitude in Implementing Cleaner Production: Case of The Pharmaceutical Industry in Jakarta

The medicines production process in the pharmaceutical industry, especially in herbal medicines, impacts natural resources and environmental pollution. Implementing cleaner production (CP) can be a profitable solution for companies. Stakeholders’ attitudes toward implementing CP are needed to achieve a sustainable pharmaceutical industry. This research was conducted at the Company X, a pharmaceutical industry that produces herbal medicines located in DKI Jakarta Province. This research aims to identify and analyze stakeholder attitudes toward CP as assessed based on knowledge, responses, and practices through a questionnaire. The sample was conducted on 122 stakeholders participating in production and environmental management activities, including the Manager, Supervisor, and Staff. The results show that all stakeholders’ knowledge, responses, and practices have a good average criteria. The most significant number of stakeholders who were respondents were production staff at 37.7%. From the results of this research, it is hoped that all respondents will know the herbal medicine production process and implement CP at Company X.

A core root bacteria contribute to plant growth and anisodine accumulation of Anisodus tanguticus

BackgroundAlthough it is well recognized that core root microorganisms contribute to plant health and productivity, little is known about their role to the accumulation of secondary metabolites. The roots of Anisodus tanguticus, a traditional herbal medication utilized by Tibetan medicine, are rich in tropane alkaloids. We collected wild A. tanguticus populations throughout a 1500 km transect on the Qinghai-Tibetan Plateau.ResultsOur results showed that despite sampling at a distance of 1500 km, the root of A. tanguticus selectively recruits core root bacteria. We obtained 102 root bacterial core OTUs, and although their number only accounted for 2.99% of the total, their relative abundance accounted for 73% of the total. Spearman correlation and random forest analyses revealed that the composition of core root microbiomes was related to anisodine contents, aboveground biomass and nitrogen contents of Anisodus tanguticus. Among them, the main role is played by Rhizobacter, Variovorax, Polaromonas, and Mycobacterium genus that are significantly enriched in roots. Functional prediction by FAPROTAX showed that nitrogen-cycling microorganisms and pathogenic bacteria are strongly associated with anisodine contents, aboveground biomass and nitrogen contents of Anisodus tanguticus.ConclusionsOur findings show that the root selectively recruits core root bacteria and revealed that the core microbiomes and microbial functions potentially contributed to the anisodine contents, aboveground biomass and nitrogen contents of the plant. This work may increase our understanding of the interactions between microorganisms and plants and improve our ability to manage root microbiota to promote sustainable production of herbal medicines.

Overview of Good Manufacturing Practices Requirements for Herbal Medicines in India and Europe

Over the past three decades, the usage of herbal medicines and supplements has grown significantly, with at least 80% of people turning to them for some aspect of primary healthcare. Although the efficacy of many herbal products has been proved, therapies utilising these compounds have showed promising potential. Since safety is still a major concern when using herbal remedies, it is crucial that the proper regulatory agencies put in place the necessary safeguards to protect public health by ensuring that all herbal medications are secure and of high enough quality. In addition to outlining some significant difficulties in conducting an efficient safety review of herbal medicinal products, this review examines toxicity-related issues and significant safety concerns resulting from their use. Good Manufacturing Practices are designed to promote human health and, as a result, quality of life. Ensure the applicability of the principles offered and demonstrate the advantages resulting from this applicability in order to accomplish the objectives specified.

A Review of Herbal Regulations and Approval Process in India and Europe

The main suppliers of herbal plants and medications are India and Europe. People prefer using plants and herbs as remedies over synthetic ones since they have fewer adverse effects. In India, AYUSH, CDSCO (Central Drugs Standard Control Organization), AND D&amp;C Act 1940 &amp; 1945(amendment) all control herbal medications. In India, AYUSH systems have been developed to house almost 8000 herbal medications. While in Europe, the European Medicines Agency (EMA) and the Herbal Medicinal Products Committee (HMPC) are in charge of overseeing herbal medications. The regulation of herbal goods in India and Europe is the subject of this essay.

Pengaruh Budaya Dan Sertifikasi Halal Terhadap Loyalitas Konsumen Jamu Ramuan Madura Di Kecamatan Kwanyar

The demand for Madurese herbal medicine products is increasing significantly in every region, this cannot be separated from Madura herbal medicine producers as providers of halal products. In this study, the object of research was the producer of Madurese herbal medicine in the Kwanyar Madura sub-district area. This research was conducted on producers to find out what factors influence the influence of culture and halal certification on consumer loyalty. This research aims to find out the process of Madurese herbal medicine producers in Kwanyar sub-district to obtain halal certification from the halal product guarantee administration body (BPJPH) using an analytical approach. multiple linear variables through cultural influence, halal certification, consumer loyalty. The method used in this research is quantitative using multiple linear analysis tests. The sampling technique is the technique used for sampling in this research with a non-probability sampling technique using a purposive sampling approach. This test consists of normality test, multicollinearity test, heteroscedasticity test, autocorrelation test, t test, f test, and multiple linear analysis.

Analisis Pengaruh Sertifikasi Halal dan Kualitas Produk Jamu Ramuan Madura Terhadap Keputusan Pembelian Oleh Konsumen di Kecamatan Modung

Analysis of the Influence of Halal Certification and Quality of Madurese Herbal Medicine Products on Consumer Interest, specifically in Kedungdung Village, Modung District, Bangkalan Regency. The aim of this research plan is to determine the influence of Halal Certification and the Quality of Madura Herbal Medicine Products on Consumer Purchasing Decisions. The type of research used is quantitative descriptive which is considered very suitable for reviewing studies that describe variables as they are and are supported by data in the form of numbers produced from actual conditions. There are various interesting facts related to halal certified Madurese herbal medicine, which in this context is still difficult to implement in Indonesia, especially in Modung District, Bangkalan Regency, this is because there are still many Madurese herbal medicines that have not implemented halal certification, especially in Bangkalan Regency.&#x0D;

Inovasi Jamu Jeli Anak : Metode Housewife Creative dengan Menggabungkan Semangat Kewirausahaan dan Pelestarian Tradisi Lokal untuk Meningkatkan Perekonomian Keluarga

The role of housewives is a vital component of the nation that can be empowered to enhance family welfare. Various programs can be implemented by women to improve family well-being by utilizing and managing their surrounding environment. One such program, particularly in the realm of food, is the cultivation and utilization of local traditions, specifically the Family Medicinal Plants (TOGA) program. Prioritized issues include the availability of land for TOGA cultivation and the processing of results that still yield conventional products, limiting their economic value. The use of chemical drugs, especially for children, poses significant risks, given the prevalence of toddlers and children under 12 in residential areas. Efforts are needed to reduce the consumption of chemical drugs in favor of herbal products and to address the innovative production and management of herbal products as a priority for PKK housewives. Activities involve socialization and training using the Housewife Creative Method as a solution. The modification of conventional herbal medicine or liquid herbal medicine into gel form is considered an innovative solution, adding economic value when sold. A 3-4 month community service project in Green Semesta Housing, Wates Subdistrict, Semarang, specifically in RT 2 RW 4, targeting PKK mothers, yielded positive and beneficial results. PKK mothers, who are also homemakers, gained additional insights into the use of TOGA plants, creating economically valuable products such as jelly herbal medicine for children in various types and creative packaging. The project also provided advanced entrepreneurial knowledge related to utilizing local products like TOGA plants. All PKK mothers could predict selling prices and produce appealingly packaged jelly herbal medicine products, making them suitable for marketing and sale.

Evaluation of antioxidant, toxicological and anxiolytic-like effect of ethanolic extracts of Ziziphus cotinifolia Reissek in adult zebrafish (Danio rerio)

BackgroundThe GABAergic and serotoninergic neurotransmission pathways are involved in the control of anxiety and ensure emotional balance. Some plant species have substances with anxiolytic activity, a therapeutic effect associated with the presence of phenolic compounds. MethodsBefore assessing the existence of anxiolytic activity, ethanolic extracts from leaves, branches and roots of Ziziphus cotinifolia Reissek were evaluated regarding biological activity and absence of toxicity. Later the influences of the samples on locomotor and anxiolytic activity, anxiolytic pathways and probable mechanisms of neurotransmission were evaluated. ResultsThe EEtFJ extract (0.5 mg/mL) did not have its anxiolytic activity reversed by the GABAergic receptor antagonist (Fmz + EEtFJ = EEjFJ; p<0.05). The twig extracts and flumazenil only partially reduced the time spent in the light zone (Fmz + EEtGJ < + EEtGJ; p<0.05), the root extract was not reversed by flumazenil (Fmz + EEtFRJ = EEtFRJ). ConclusionThe initial analysis data reveal the presence of phenolic compounds that explain the biological activities found and, as they act on different targets of neurotransmission systems involved in the control of anxiety disorders, they have potential for the production of herbal medicines with synergistic action.

Decontamination protocol in the production of herbal medicines: a short communication

Background: The effectiveness and safety of natural products depend not only on the inherent properties of the plant, but also on careful preparation, which includes critical steps such as cleaning to remove impurities and washing to eliminate surface contaminants. Thus, the scientific literature emphasizes the significance of thorough cleaning in ensuring botanical purity, directly impacting the ultimate quality of the product. Methods: The study utilized the plant species Cordia verbenacea, Alternanthera brasiliana, and Lippia alba, cultivated in Tatuí - SP, subjected to a rigorous selection process, and processed through a washing and drying protocol. The washing involved a two-stage process, including immersion in clean water and a sodium hypochlorite solution. Microbiological analyses were conducted on washed and unwashed samples, including enumeration of viable colony-forming units (CFUs) and detection of pathogens. Moisture content was determined using the oven drying method, and ash content was assessed following the Brazilian Pharmacopeia guidelines. Results: Microbiological results indicate the absence of Salmonella sp. and Staphylococcus aureus in all analyzed plant drugs, regardless of washing and stabilization conditions. Pseudomonas aeruginosa was observed only in unwashed/stabilized Cordia verbenacea and Alternanthera brasiliana samples. Escherichia coli was identified in an unwashed/stabilized Lippia alba sample. The washing/stabilization process significantly reduced total bacterial and fungal counts, especially when exceeding the limit set by the Brazilian Pharmacopeia (103 CFU/g). Total ash content remained unchanged after the washing/stabilization process in the three plant species, staying within the Brazilian Pharmacopeia limits (14%). Moisture content did not differ statistically between washed/stabilized and unwashed/unstabilized plant species and met Brazilian Pharmacopeia specifications (8-14%). Conclusion: The washing/stabilization process effectively reduced microbial load in Lippia alba, Alternanthera brasilians, and Cordia verbenacea, with no significant changes in ash and moisture content. Preliminary analysis suggests that processing control ensures minimum quality conditions, enhancing overall safety for consumers.