Medical Importance Research Articles

Identification and characterization of a novel Pavlovskyella (Acari: Argasidae) from Chile, parasite of the culpeo fox (Lycalopex culpaeus).

Soft ticks (Argasidae) of the subgenus Pavlovskyella Pospelova-Shtrom are worldwide distributed parasites of medical importance. However, the systematics of the subgenus are currently under debate because genetic data shows that the group is paraphyletic. Meanwhile, species of Pavlovskyella continue to be discovered. In this study a novel species of the subgenus is described from specimens collected on a fox in central Chile. The larva of this new species differentiates from other Pavlovskyella spp. by having the following combination of characters: subpyriform dorsal plate; 15 pairs of setae, 7 anterolateral, 3 central, and 5 posterolateral, and hypostome with denticles in the distal third. Nymphs and adults of the species lack cheeks, eyes or bulging structures on the flank, but exhibit dorsoventral grooves, and humps on tarsi I, II, and III. Moreover, a patch of glabrous integument appears on the distal portion of coxal folds. A phylogenetic analysis using the mitogenome indicates a monophyletic group composed by Ornithodoros (Pavlovskyella) brasiliensis Aragão, Ornithodoros (Pavlovskyella) furcosus Neumann, Ornithodoros (Pavlovskyella) improvisus Muñoz-Leal & Venzal, and Ornithodoros (Pavlovskyella) rostratus Aragão. Interestingly, a phylogeny using 18S-28S rDNA sequences shows that South American Pavlovskyella spp. are paraphyletic, as depicted in previous studies. Adding species of the subgenus from the Neotropical region to phylogenetic analyses could aid to solve this paraphyly. Furthermore, this is the fifth species of Pavlovskyella described in South America, and the second in Chile.

Covid-19, the Eucharist and spreading disease through Christianity’s holiest rite

During the Covid-19 pandemic in the USA, churches were faced with a question of medical and metaphysical importance: can Christianity’s rite of Holy Communion spread Covid-19? This article examines Christian responses to Covid-19 through a case study of five of Christianity’s multifaceted Covid eucharistic policies, arguing that the responses of churches in times of crisis reflect a nuanced understanding of the metaphysical theories surrounding the Eucharist. The goal of this article is to serve as a primer on the relationships between beliefs of the Eucharist and Covid-19 in American churches, seeking to provide an overview and facilitate further inquiry into modern concerns of Christianity’s holiest rite and the spread of disease.

Toscana virus - an emerging Mediterranean arbovirus transmitted by sand flies.

Toscana virus (TOSV) is an emerging arthropod-borne virus (arbovirus) of medical importance that is increasing its range across much of the Mediterranean Basin, Europe and the Middle East. Transmitted by Phlebotomus spp. sand flies, it is the most clinically relevant sand fly-borne phlebovirus. Initially isolated in the Tuscany region of Central Italy, it has now been detected in multiple countries that surround this geographical area. Infection of the vertebrate host can cause fever and neurological disease, following the dissemination of the virus to the brain. The prevalence is high in some regions, with a notable percentage of individuals showing seroconversion. TOSV can be a leading cause of acute meningitis and encephalitis (AME) during the summer months. In this comprehensive review, we will focus on several key topics. We discuss how TOSV has spread to establish outbreaks of infection in both humans and animals around the Mediterranean and the wider region. Clinical aspects of TOSV infection in humans are described, along with the best standards in diagnosis. Finally, we focus our discussion on the role of the sand fly vector, describing their biology, vector competency, implications for putative vertebrate reservoirs, the effect of the climate emergency on sand fly distribution and the putative role that sand fly-derived salivary factors may have on modulating host susceptibility to TOSV infection.

NEW RECORDS OF MOSQUITO SPECIES FROM NORTHERN ARGENTINA.

Changes in the ecosystems of vector-borne diseases can make the environment more or less favorable for mosquito vectors, animal reservoirs, and disease transmission. We report for the 1st time Anopheles marajoara in Argentina. In addition, An. deaneorum is reported for the 1st time in northwestern area of the country. Aedes fulvus, Culex eduardoi, Limatus durhamii, Mansonia pseudotitillans, Psorophora albigenu, Ps. cingulata, and Uranotaenia nataliae are recorded for the 1st time in Tucumán Province. Data on collection localities and comments about medical importance are also presented.

Bacterial Communities of House Flies from Beef and Dairy Cattle Operations are Diverse and Contain Pathogens of Medical and Veterinary Importance.

Adult house flies (Musca domestica L.) are important reservoirs and mechanical vectors of bacteria in livestock operations. House fly bacterial communities are influenced by their local environment, yet a comprehensive understanding of bacterial diversity, pathogen prevalence, and bacterial source is not fully understood. We characterized bacterial communities from adult female house flies and associated manure samples from beef and dairy cattle farms in Kansas, Oklahoma, and Texas over four months (July-October). Bacterial community composition in flies and manure reflected the local environment, and house flies shared the majority (≥ 99%) of bacterial taxa with manure. The variability of bacterial diversity was greater among individual fly (species richness range: 48-1747) samples than manure (species richness range: 345-1162). Temporal variability of fly bacterial diversity was observed within each farm type. Bacterial taxa of veterinary and medical importance such as Corynebacterium, Turicibacter, Staphylococcus, Streptococcus, and Acinetobacter were highly prevalent in flies, constituting core bacterial communities. The prevalence of bacterial taxa associated with bovine keratoconjunctivitis (IBK) and bovine respiratory disease (BRD) was higher in flies than in manure and prevalence varied monthly. This study underscores the crucial role house flies play as carriers of cattle pathogens, contributing to their dissemination among animals and to off-site locations, where they pose a threat to surrounding communities and agricultural operations.

Evaluation of TNF-α Concentration Level in Al-Najaf Hospitals Patients Infected with Hydatid Cysts

Human echinococcosis is a zoonotic disease caused by a species of medical importance, the Echinococcus granulosus , an etiological agent of cystic echinococcosis (CE). A radiological examination will be performed for these patients to diagnose Echinococcus granulosus parasite by find echinococcal cysts. All of these patients were involved in this study and during the period starting from October 2023 to the end of January 2024, from all ages of patient from both sex (Males and Females At Al-Sader Medical City, AL-Hakeem General Hospital, AL-Haidarya general Hospital and Al-Hayat Hospital. The mean level of TNF-α in patients is 163.27 pg/ml, which is significantly higher than the mean level of 38.58 pg/ml in controls (p-value < 0.001). The prevalence of hydatid disease in Al-Najaf Al-Ashraf in this study was 33%. Levels of the cytokines IL-6, TNF-α, IL-1ß, and IL-4 are more important in patients with Echinococcus granulosus under 40 years of age than in those over 40 years of age.

Educating lifestyle modification to diabetic patients

The burden of non-communicable diseases (NCDs) is steadily increasing, making them a major health concern. Diabetes mellitus (DM) is one of the major non-communicable diseases that affects the public health and the community. DM is a chronic, complex disease that requires multi-factor risk reduction strategies apart from its maintaining blood glucose level. One of the community-based strategy is to educate diabetic patients about life style modifications. Keeping that as a guideline, a nurse-led intervention was carried out to ring about a change in life style in these patients. About 60 diabetic patients with equal number of control subjects were taken for the pilot study. Data were collected through interviews with a semi-structured questionnaire before (pre-test) and after nurse-led intervention (post-test) The data were analyzed using descriptive and inferential statistics; the calculated data were tabulated and graphed. The results of the study show that diabetic patients included in the study have improved their knowledge of life style modification. The community nurse intervention improved their perspective and understood the importance of life style medication (from 16.19 to 24.98%). This was tested using the student paired t test. Outcome of the test before the intervention showed significant change. Pre-test value was (53.97%) and the post-test intervention was 83.27%. The effect of such intervention was influenced the age, gender, education, and family structure. The intervention was found to be more effective in male patients of the age group 30-40 years with educational background and stable family structure having a joint family. The awareness created by nurse led intervention is effective and is significant taking age, sex, education level and the family structure. The pilot study indicates that further studies may be carried out in the community with general population and then specifically in patients with diabetes.

Tulsi's Ayurvedic Medical Importance

Tulsi (Ocimum sanctum) is an omnipresent, multipurpose plant and regarded as holy plant in Hindu religion finds place in front of every Hindu household. This herb is a member of Lamiaceae family, well known for its spiritual, religious and medicinal importance in India. Scientific investigations of traditional belief of medicinal properties of Tulsi have got impetus mostly in the later part of the 20th century. Characteristically, the plant imparts a pungent, bitter, hot, light and dry effect indicating the presence of curative value. It is extensively famous and has been used since centuries in Ayurveda and Greek medicines owing to its beneficially diverse properties. For the treatment of bronchitis, illness, and pyrexia, tulsi leaf extracts are described in Indian medical literature. It is thought to be a widespread plant in India.

Culicoides biting midges in urban areas of northern Spain.

Culicoides biting midges (Diptera: Ceratopogonidae) are known vectors of multiple arboviruses of veterinary and medical importance, as well as avian haemosporidian parasites. Despite their significant role as vectors, the distribution of Culicoides species in non-farmland habitats remains largely unknown. We conducted a study to assess the species community composition, abundance, and host feeding patterns of Culicoides biting midges in urban ecosystems in northern Spain. Light-baited suction traps were operated biweekly for 6 months across six urban landscapes (three cemeteries and three green areas). In total, 181 Culicoides specimens of 18 species were morphologically identified, with the Obsoletus complex being predominant (43.% of the total catches), followed by Culicoides kibunensis (21.5%). Culicoides midges peaked in early June in green areas and in May in cemeteries. Host feeding patterns revealed avian preferences (mainly Turdus spp.) in four Culicoides species. Despite the low overall number of specimens collected, a high diversity of biting midge species was recorded. These findings offer critical insights into Culicoides ecology and their interactions with hosts in urban settings, which are vital for disease surveillance and management strategies.

A chromosome-level genome assembly for the desert scorpion Mesobuthus przewalskii from Asian drylands.

Scorpions are an ancient and charismatic group of arthropods with medical importance, but a high-quality reference genome for this group is still lacking. Here we perform whole-genome sequencing of Mesobuthus przewalskii, a desert scorpion endemic to the Taklimakan Desert. We combine PacBio HiFi sequencing and Hi-C chromosome conformation capturing to generate chromosomal-level, haplotype-resolved, and fully annotated genome assembly for this medically important scorpion. The assembly consists of two haplotypes (1052.01 Mbp and 1055.19 Mbp, respectively) reaching chromosome-level contiguity and >98% BUSCO completeness. Sequences were anchored in 13 chromosomes with a contig N50 of 34.44 Mbp and scaffold N50 of 81.43 Mbp. Several key genome features and the mitochondrial genome assembly were also provided. This genome represents the fifth but the most complete assembly for the order Scorpiones.

Nematocyst Types and Characteristics in the Tentacles of Gershwinia thailandensis and Morbakka sp. (Cubozoa: Carybdeida) from the Gulf of Thailand.

Nematocysts, specialized stinging cells in cnidarians, play a crucial role in both defense and prey capture, containing venomous, coiled tubes within a capsule. While box jellyfish are recognized as a medical threat, information on the nematocysts of species like Gershwinia thailandensis and Morbakka sp. from Thai waters remains sparse. This study explores the types and morphology of nematocysts found in the tentacles of these species using light and scanning electron microscopy. We identified three nematocyst types: club-shaped microbasic p-mastigophores, oval isorhizas, and oval microbasic p-rhopaloids. Notably, significant differences in capsule sizes were observed, especially in the microbasic p-mastigophores and isorhizas. The discharge tubules tapered from the proximal to the distal ends, featuring arrow-shaped spines in a helical pattern. A distinct lancet structure was present in both microbasic p-mastigophores and p-rhopaloids. These findings suggest that variations in nematocyst size and morphology may be linked to evolutionary adaptations, functional roles, and venom toxicity. Further research into venom discharge mechanisms could offer valuable insights into the ecological and medical importance of these cnidarians.

Intraspecific venom variation in the medically important puff adder (Bitis arietans): Comparative venom gland transcriptomics, in vitro venom activity and immunological recognition by antivenom.

Variation in snake venoms is well documented, both between and within species, with intraspecific venom variation often correlated with geographically distinct populations. The puff adder, Bitis arietans, is widely distributed across sub-Saharan Africa and into the Arabian Peninsula where it is considered a leading cause of the ~310,000 annual snakebites across the region, with its venom capable of causing substantial morbidity and mortality. Despite its medical importance and wide geographic distribution, there is little known about venom variation between different B. arietans populations and the potential implications of this variation on antivenom efficacy. We applied a range of analyses, including venom gland transcriptomics, in vitro enzymatic assays and reverse phase chromatography to comparatively analyse B. arietans venoms originating from Nigeria, Tanzania, and South Africa. Immunological assays and in vitro enzymatic neutralisation assays were then applied to investigate the impact of venom variation on the potential efficacy of three antivenom products; SAIMR Polyvalent, EchiTAb-Plus and Fav-Afrique. Through the first comparison of venom gland transcriptomes of B. arietans from three geographically distinct regions (Nigeria, Tanzania, and South Africa), we identified substantial variation in toxin expression. Findings of venom variation were further supported by chromatographic venom profiling, and the application of enzymatic assays to quantify the activity of three pathologically relevant toxin families. However, the use of western blotting, ELISA, and in vitro enzymatic inhibition assays revealed that variation within B. arietans venom does not appear to substantially impact upon the efficacy of three African polyvalent antivenoms. The large distribution and medical importance of B. arietans makes this species ideal for understanding venom variation and the impact this has on therapeutic efficacy. The findings in this study highlight the likelihood for considerable venom toxin variation across the range of B. arietans, but that this may not dramatically impact upon the utility of treatment available in the region.

DESAFIOS NA ADESÃO MEDICAMENTOSA SOB A PERSPECTIVA DOS PROFISSIONAIS DE SAÚDE DA ATENÇÃO PRIMÁRIA

This study explores the factors influencing medication adherence among patients of the Family Health Strategy (ESF) in Embu das Artes/SP, based on the perspectives of healthcare professionals. The qualitative research, which involved semi-structured interviews and a literature review, identified low health literacy among patients as the primary barrier. Adherence to treatment is influenced by internal factors, such as patient perception and motivation, as well as external factors, including socioeconomic status and cultural aspects. The lack of understanding regarding the importance of medications and the chronic nature of diseases exacerbates the problem, leading to elevated risks such as mortality and hospital readmission. Professionals reported challenges such as fear of side effects and confusion over medication schedules. These issues underscore the need to enhance healthcare professionals' training to develop educational strategies aimed at providing health literacy and achieving more effective treatment adherence.

Spatial transcriptomics of a parasitic flatworm provides a molecular map of drug targets and drug resistance genes

The spatial organization of gene expression dictates tissue functions in multicellular parasites. Here, we present the spatial transcriptome of a parasitic flatworm, the common liver fluke Fasciola hepatica. We identify gene expression profiles and marker genes for eight distinct tissues and validate the latter by in situ hybridization. To demonstrate the power of our spatial atlas, we focus on genes with substantial medical importance, including vaccine candidates (Ly6 proteins) and drug resistance genes (glutathione S-transferases, ABC transporters). Several of these genes exhibit unique expression patterns, indicating tissue-specific biological functions. Notably, the prioritization of tegumental protein kinases identifies a PKCβ, for which small-molecule targeting causes parasite death. Our comprehensive gene expression map provides unprecedented molecular insights into the organ systems of this complex parasitic organism, serving as a valuable tool for both basic and applied research.

The Toxin Diversity, Cytotoxicity, and Enzymatic Activity of Cape Cobra (Naja nivea) Venom.

"True" cobras (genus Naja) are among the venomous snakes most frequently involved in snakebite accidents in Africa and Asia. The Cape cobra (Naja nivea) is one of the African cobras of highest medical importance, but much remains to be learned about its venom. Here, we used a shotgun proteomics approach to better understand the qualitative composition of N. nivea venom and tested its cytotoxicity and protease activity as well as its effect on intracellular Ca2+ release and NO synthesis. We identified 156 venom components representing 17 protein families, with the dominant ones being three-finger toxins, mostly of the short-chain type. Two-thirds of the three-finger toxin entries identified were assigned as cytotoxins, while the remainder were categorized as neurotoxins, including short-chain, long-chain, and ancestral three-finger toxins. We also identified snake venom metalloproteinases and members of CRISP, l-amino acid oxidase, and other families. Protease activity and its effect on intracellular Ca2+ release and NO synthesis were low. Phospholipase A2 activity was surprisingly high, despite this toxin family being marginally recovered in the analyzed venom. Cytotoxicity was relevant only at higher venom concentrations, with macrophage and neuroblastoma cell lines showing the lowest viability. These results are in line with the predominantly neurotoxic envenomation symptoms caused by Cape cobra bites. The present overview of the qualitatively complex and functionally intriguing venom of N. nivea may provide insights into the pathobiochemistry of this species' venom.

An Ecological Study of the Brown Garden snail Cornu aspersum (Müller, 1774) from selected habitats in Jadiriya in Baghdad vicinity.

The present study is considered a pioneer investigation that deal with the terrestrial brown garden snail Cornu aspersum (Müller), in Iraq. Cornu aspersum however is considered an exotic species in many parts of the world ,The species is most probably infiltrates with plants transferred from one place to another. The species has gained importance in many ways. Nutritionally, the species is consumed as food item in many countries, but in other cases it is considered as pest for the damage it causes when feeding on valuable plant shoots. It also has medical importance for its role and ability in healing wounds, burns and remedy of other skin problems. This snail species however may act as a vector for some parasitic nematodes that cause serious diseases. The species is also important in scientific research, usually referred to as ‘ecological indicator’ employed in environmental pollution problems such as the one caused by heavy metals. A great deal of research on this species has been carried out in different parts of the world, however, in Iraq, the scientific attention given to land snails in general and to Cornu aspersum in particular is rare and may be negligible in comparison with the research carried out on aquatic Mollusca species. A total of 1880 individuals of Cornu aspersum were collected from all sampling sites. The study was carried out for the period November 2007 to November 2008 on three sites within the Jadiriya district in Baghdad.. Growth, size classes and shell dimensions of Cornu aspersum populations, and their relationship with major environmental factors; ambient temperature; soil pH, moisture, texture; and the soil content of organic matter have been also investigated. Identification of the species was confirmed by the British Natural History Museum.The data were analyzed by means of standard statistical procedures.

Detection of Filariid Infections in Mexican Primate Populations Through qPCR.

Filariae are parasitic nematodes of high veterinary and medical importance, responsible for some acute tropical diseases. They are transmitted through the bite of hematophagous vectors such as biting midges and blackflies. Filariae are among the most prevalent vector-borne parasitoses in Neotropical primates in which severe infections can cause inflammatory reactions and tissue damage. Given the location inside the host (peritoneal cavity, bloodstream, and lymphatics), the detection of filariid nematodes is challenging and is mostly postmortem; hence the scarcity of studies on the prevalence of filariae in wild primate populations. Here, we report the prevalence of filariid infections in free-ranging populations of Geoffroy's spider (Ateles geoffroyi) and black howler (Alouatta pigra) monkeys across southern Mexico, using a combination of noninvasive sampling and molecular diagnostic techniques. Fecal samples were screened for filariid DNA by qPCR protocols. A total of 88 samples were examined with an overall prevalence of 26%. Filariae were slightly more common in spider monkeys compared to howler monkeys. This study constitutes the first report of the prevalence of infection of filariid nematodes in populations of wild spider monkey across southern Mexico, and the first reporting of filariae in black howler monkeys, as part of a new era of primate parasitology and the diagnostics of parasite infections in light of the everyday more affordable molecular tools.

Phosphodiesterase4 inhibitors in dermatology : Role in the treatment of skin diseases

Chronic inflammatory skin diseases are of great social and medical importance and require effective drug therapy. Phosphodiesterase4 (PDE4) inhibitors represent apossible therapeutic option by regulating inflammatory processes. PDEs cause the release of proinflammatory cytokines by interfering with signaling pathways. The PDE4 inhibitors apremilast (treatment of psoriasis and Behçet's disease), roflumilast (treatment of chronic obstructive pulmonary disease), and crisaborole (treatment of atopic dermatitis) are currently approved in Europe. Apremilast is used for second-line treatment of plaque psoriasis and psoriatic arthritis and has afavorable side effect profile. Topical PDE4 inhibitors are currently being researched and have not yet been approved by the European Medicines Agency (EMA). The topical PDE4 inhibitor crisaborole was approved by the EMA in 2020 as atopical treatment alternative to glucocorticoids and calcineurin inhibitors. Although the substance has shown good tolerability in studies and also alleviates the accompanying itching, it did not find its way onto the German market. BEHçET'S DISEASE: Apremilast is approved for the treatment of Behçet's disease in adults with refractory, severe oral ulcers. Case studies have also demonstrated the efficacy of systemic PDE4 inhibition in other skin diseases (including blistering autoimmune dermatoses, lichen planus, and acantholytic genodermatoses). The substances are also being researched and used to treat extracutaneous inflammatory diseases.

Co-introduction into a delicate island ecosystem: metastrongyloid nematodes (superfamily Metastrongyloidea) of veterinary and medical importance circulating in aquatic and terrestrial environments of Tenerife (Canary Islands).

Metastrongyloid nematodes typically reside as adults in the cardiopulmonary systems of their mammalian definitive hosts, potentially causing severe diseases. Of particular concern are Angiostrongylus cantonensis and A. costaricensis, which can cause eosinophilic meningitis and abdominal angiostrongyliasis, respectively, in their accidental human hosts. Several metastrongyloid species of medical and veterinary importance have been documented in the Canary Islands. However, the gastropod species acting as intermediate hosts for some of these nematodes in the archipelago remained unknown. This study aimed to investigate the occurrence of metastrongyloid nematodes in terrestrial and aquatic gastropods, including both endemic and non-native species, on Tenerife. Foot samples from terrestrial and aquatic gastropods were analyzed using a multiplex PCR targeting the Internal Transcribed Spacer 1 (ITS1), allowing the specific detection of A. cantonensis, A. vasorum, Aelurostrongylus abstrusus, Crenosoma striatum, Troglostrongylus brevior, and Crenosoma vulpis. Five metastrongyloid species, namely C. striatum, A. cantonensis, Ae. abstrusus, A. vasorum, and an unidentified metastrongyloid, were identified within both non-native and endemic terrestrial gastropods. In the aquatic snail Physella acuta, only A. cantonensis and C. striatum were detected. This study confirms the introduction of various metastrongyloids associated with non-native mammalian fauna and provides new data on the occurrence of these nematodes in non-native and endemic gastropod species, including their presence in aquatic environments on the Canary Islands.

Assessment of enteric helminth parasites in bushmeat in Ghana

Anthropogenic activities, such as hunting wild animals for food, increase the risk of zoonotic transmission of infective stages of parasites to humans. The handling, processing and consumption of wild animal meat, popularly known as ‘bushmeat’, as well as exposure to wildlife habitats, can pose a significant risk to human health through the transfer of parasitic infective stages. This study sought to assess the enteric helminth parasite burden and potentially zoonotic helminths in fresh, wild animal carcasses being processed for food. Parasitological analysis of samples of rectal and intestinal contents collected from a total of fifty (50) wild animal carcasses belonging to eight (8) different species at the Atwemonom Bushmeat Market in Kumasi showed nine (9) genera of enteric helminth parasites with an overall prevalence of 71.0%. Individual parasite species prevalence was assessed, with Ascaris sp. showing 25% and 87.5% by coprological and molecular assessment, respectively. Molecular analysis showed a higher parasite species prevalence in all samples analyzed. Species-specific analysis indicated the presence of two potentially zoonotic parasites, Strongyloides stercoralis and Trichuris trichiura, in wild animals, indicating the need to intensify one health approach in wild animal parasitic infections. Data from this study suggest that wild animals in addition to being natural hosts, may also serve as reservoirs for numerous parasites of medical and veterinary importance.