Medical Science Center Research Articles

Clinical and radiological outcome of lateral end clavicle fractures treated with locking compression plate: Prospective study of 19 cases

Background & Objectives: Roughly a quarter of all clavicle fractures occur at the lateral end. Displaced fractures of the lateral end clavicle have a higher rate of nonunion. The management of fractures of the lateral end clavicle remains controversial. Open reduction internal fixation with a superiorly placed locking plate is a recently developed technique. However, there are no randomized controlled trials to evaluate the efficacy of this procedure. We present a series of nineteen cases which highlight this method of fixation for lateral end clavicle fractures. The objectives were to assess fracture stability, ac joint subluxation and functional outcome. The LCP is a single beam construct where the strength of its fixation is equal to the sum of all screw-bone interfaces rather than a single screw’s axial stiffness and pull out resistance in unlocked plates. Its unique biomechanical function is based on splinting rather than compression resulting in flexible stabilisation, avoidance of stress shielding and induction of callus formation. Further, when it is applied via a minimally invasive technique, it allows for prompt healing, lower rates of infection and reduced bone resorption as blood supply is preserved. Methods: This study is a prospective, time bound, hospital based study conducted in Kempegowda Institute of Medical Sciences And Research Center, Bangalore, between May 2013 to May 2015. The study included 19 cases of lateral end clavicle fractures that were operated with the Locking compression plate. The fractures were classified according to Neer’s classification and were followed up at regular intervals. Clinical and radiological parameters were noted. Final functional outcome was assessed using Karlsson’s criteria for assessment of postoperative shoulder functional recovery. Results: Good reduction was achieved in 90% of cases .17 (89%) male and 2 (11%) female patients. Average age of the patients were between 20-45 years. Type of injury was road traffic accidents 10 (54%) cases and self-fall 9(46%) cases. All fractures were simple in nature.13 cases were Neer’s type IIA & 6 cases were Neer’s type IIB. Conclusion: Locking compression plate is a good fixation system for lateral end clavicle fractures. Operative time is reduced and surgical dissection is minimum. Device provides good angular stability and helps in early mobilization.

Prediction of Psychological Welfare Based on Defensive Mechanisms and Coping Strategies in People with Positive HIV

AIDS, in the last century the main threat to the health of people worldwide. In fact, after seeing the first cases of HIV in 1981, Discuss the mechanism of action to identify, control and treat it with the speed of the American Medical community in Los Angeles and then spread to other parts of the world. But the pace of HIV, most of their efforts so that to this day, according to the World Health Organization, Every minute, 6 people in the world due to HBV infection, lose their lives (Owen, 1999). The ability to manipulate the deadly nature of this disease is very high, runaway prevalence in all countries, and therefore very heavy losses, Has made in a decade, all the countries in coordination with international organizations unite against this disease even in 2000, the UN Security Council to meet to assess the situation (Hubble, 2003). It is believed that AIDS and HIV infection cause a huge psychological and social pressures for the individual patient, the family, especially their wives and professionals and health care providers these people. This is for the person with psychological and social pressures for such reactions: disbelief, denial, anger, irritability, anxiety and apprehension, fear and depression, Suicidal thoughts, poor health, social isolation and complain, behaviors, frustration, low self-esteem and cognitive deficits (by engaging the central nervous system infections caused by the disease) occur. This shows that the quality of life in people with HIV is low (Arenda, 2004). Suicidal thoughts, poor health, social isolation and complain, frustration, low self-esteem and cognitive deficits (by engaging the central nervous system infections caused by the disease) occur. This shows that the quality of life in people with HIV is low (Arenda, 2004). Curtis (2000) suggests that psychological factors may affect the vulnerability of a person for HIV positive. The role of psychological factors and interventions by encouraging healthy positive beliefs and behaviors may improve quality of life, reduce stress and overall psychological well-being and help people with knowledge of the matter (according to the Pirasteh, Motlagh, & Nikmanesh, 2012). The present study tried to predict psychological well-being, according to defense mechanisms and coping strategies in HIV positive people. Seems to be addressing the issue of HIV-positive people and identify the factors affecting their psychological well-being able to achieve the necessary information And for proper planning and provision of services tailored to the needs of these people in order to improve their psychological well-being, As well as individual awareness of the consequences of the disease and preventive programs by the scientific and medical communities due to its increasing prevalence, including the necessity of using this research. Of course, if HIV is detected predictors of psychological well-being of individuals becomes the easier way to deal with problems arising from it. Therefore, identifying factors that can be psychological well-being of people with hiv predict, In prevention, treatment and care interventions should be considered. This research can help people with AIDS face many problems in dealing with the disease, to be used.Statistical societyHIV+population consisted of all patients with psychological counseling center in Shiraz University of Medical Sciences in the year 1393 is. Inclusion criteria include: developing HIV + diagnosis treatment center in Shiraz University of Medical Sciences, crossing more than three months from the knowledge of a person from getting HIV +, at least elementary education.MethodThe study was descriptive and correlational.ParticipantsSampling method is purposive sampling total of 100 HIV + patients, who come to the University Psychological Counseling Center, were selected.InstrumentsStudy, three questionnaires were used to collect information that include:Psychological well-being questionnaire: Psychological well-being questionnaire by Reef (1995) University of Wisconsin Medical Science Center was built in 2002, was revised. …

True Parapharyngeal Space Tumors: Case Series from a Teaching Oncology Center.

The Parapharyngeal Space (PPS) tumors are rare tumors accounting for 0.5% of the tumors in head and neck region. A retrospective study conducted at Department of Surgical oncology, Vydehi institute of Medical Sciences & Research center, Bengaluru, between 2010 and 2015 identified nine cases treated for PPS tumors. Patients are diagnosed on the basis of clinical examination fine needle aspiration cytology and imaging and considered for excision by one of the varied surgical approaches {transoral(1), transcervical(4), transparotid(0), transcervical-transparotid(1), transmandibular(3) or intratemporal(0) approach}. The choice of approach is defined by the size of the tumor, suspicion of malignancy and the position of the tumor with regard to the superior extent and proximity to the skull base as well as its relation with neurovascular bundle.

Bacterial Contamination of Adult House Flies (Musca domestica) and Sensitivity of these Bacteria to Various Antibiotics, Captured from Hamadan City, Iran.

House flies (Musca domestica) have been known as a mechanical vector in spreading infectious diseases such as cholera, shigellosis, salmonellosis and skin infections. To investigate the bacterial contaminations of house flies and determine the resistance of these bacteria against various antibiotics. An analytical descriptive cross- sectional study was conducted. The study was performed from July to September 2015 in Hamadan University of Medical Sciences, Hamadan, Iran. A total number of 300 house flies were collected from four places, 75 flies from each place, including two educational hospitals belonging to Hamadan University of Medical Sciences, a fruit and vegetables center, and a livestock slaughter. The body surface of house flies was washed using the physiological sterile serum and the obtained solution was centrifuged at 2000 rpm for five minutes. The identification of bacteria was carried out using the phenotypic methods. The resistance of bacteria against various antibiotics was determined using the disk diffusion approach. Data were analysed by the employment of SPSS software package version 20.0. A total number of 394 bacterial strains were isolated from 275 house flies. The most prevalent type of bacteria was Bacillus spp which was detected in 31.1% of house flies. Moreover, Staphylococcusspp. (22.9%), Escherichiacoli (11.6%) were other prevalent species, whereas, Enterococcusspp. was the least prevalent type of bacteria in the collected house flies. In terms of resistance to antibiotics, it was identified that bacteria extracted from house flies which were collected from hospital environments were more resistant to antibiotics compared with the resistance of bacteria extracted from house flies which were collected from non hospital environments. The maximum bacterial isolation was found in houseflies from hospital No.1 environment from around the accumulation of garbage. It is a well-known fact that house flies are a source of bacterial contamination and can act as a mechanical carrier and cause bacterial diseases. It can be postulated that house flies play a major role in spreading antibiotic resistant bacteria. However, the flies from hospital environments were more contaminated, mainly because the people referring to health center are normally ill and carrier of many pathogens. Further, hospital environments should be controlled using administrative procedures.

A Prospective Study on Type 2 Diabetic Patients having Cutaneous Manifestations in a Tertiary Care Hospital in Rajsamand

Background:Diabetes mellitus is now affecting individuals of all age group and socioeconomic status. Besides other complications of the diabetes mellitus, skin is affected by the acute metabolic derangements as well as by chronic degenerative complications of diabetes. To evaluate the prevalence of skin manifestations in patients with diabetes mellitus. To analyze the prevalence and pattern of skin disorders among diabetic patients in North part of Uttar Pradesh. Methods: This Prospective study was conducted on 40 diagnosed Type 2 diabetic patients having skin lesions attending the Department of Medicine, Ananta Institute of Medical Sciences & Research Center, Rajsamand, Rajasthan were included in our study on the basis of inclusion and exclusion criteria. Out of 40 patients, only 25 patients were completed the whole study done during the duration of 3 months (March 2016 to May 2016. Results: The common skin disorders were: diabetic dermopathy (44%), Xerosis (36%), skin tags (32%), cutaneous infections (31%), and seborrheic keratosis (30%). Conclusions: Skin is involved in diabetes quite often and the manifestations are numerous. Most of the patients were affected from Diabetic dermopathy.

Relationship of Respiratory Diseases and the Lead Level in Tiran & Karvan Region, Iran

Abstract Aims: Lead is emerged as one of the most hazardous elements, existing in the air and soil, which can create irreparable outcomes in the human body due to its highly severe damages. the aim of this study was to determine the relationship between Lead level and the respiratory diseases in Tiran & Karvan Region, Iran. Instrument & Methods: The research methodology was analytical based on the documents and evidence, and library studies and was performed during 2011 to 2015 in Tiran & Karvan Region, Isfahan Province, Iran. The respiratory diseases statistics regarding the studied area was gathered from the information and statistics of Isfahan University of Medical Sciences and Iran Management Center for Diseases during 2007 to 2009 documents (3215 cases). 110 soil samples and 55 water samples were collected from different positions at Summer 2011, randomly. The variation map of the respiratory diseases and lead spatial distribution for research area were drawn by Arc GIS 10.2 software. Findings: The concentration of lead was 59.4±7.9ppm in soil and 48.2±4.1ppm in water of the region. The lead variation map showed that the soil in the eastern, northern and western areas consists of a relatively high portion of lead. Also special distribution of the respiratory diseases in Tiran & Karvan showed that the disease variation is in a cluster manner and that the disease follows a special centralization. Conclusion: There is a direct relation between respiratory diseases distribution and the existence of lead in Tiran & Karvan Region, Isfahan Province, Iran.

A Prospective study of surgical management of diaphyseal fractures of femur & tibia in children aged between 5 to 16 years using elastic stable intra medullary nailing

Introduction: Elastic stable intramedullary nailing for the treatment of pediatric femur & tibia diaphyseal fractures was introduced by pr´evot and colleagues in 1979. The technique offers several advantages, including better reduction, dynamic axial stabilization, shorter hospitalization with early rehabilitation and low rate of complications. Methods: 30 children between ages 5 to 16 years admitted to the department of Orthopedics at Sapthagiri institute of medical science and research center, Bangalore with diaphyseal fractures of femur & tibia during the period from November 2014 to February 2016 are selected. Results: Our series consisted of 30 patients, 18 femur & 12 tibia. 24 male and only 6 female. Most common fracture pattern of femur & tibia was spiral. 23 fractures healed within an average duration of 10 to 12 weeks following surgery. There was superficial infection in 1 case, 2 cases had lld

A prospective study of osteosynthesis of malleolar fractures of ankle in adults

Introduction: In this age of road traffic accidents and sports activities the occurrence of malleolar fractures of the ankle are common. Malleolar fractures account for nearly 2% of all the fractures. Methods: During the period of July 2015 to July 2016, thirty adults with malleolar fractures of the ankle were operated at Sapthagiri Institute of Medical Science And Research Center, Bangalore. They were treated by open reduction and internal fixation by one-third tubular plate or dynamic compression plate, tension band wiring, malleolar screws or a combination of these. The rating was done as per Weber’s protocol. Results: Acceptable results were obtained in twenty- five out of thirty patients, with 83.3 % of the patients having excellent to good rating. Five patients (16.6%) results were poor, because of pain on activity, restriction of range of motion at the ankle and subtalar joint, and restriction of activity. Conclusion: Surgical management of malleolar fractures of the ankle by open reduction and internal fixation remains the treatment of choice, to ensure anatomical joint restoration, union, and good function of the ankle joint.

Management of gun shot injury in a tertiary hospital

Introduction: Gun shot injury is an uncommon surgical emergency . A timely and multidisciplinary management by surgical team is an essential factor in decreasing mortality and morbidity . This study highlights the multidisciplinary management of gun shot injury.Methodology: Bir hospital is a postgraduate teaching hospital under National Academy of Medical Sciences and tertiary referral center in Kathmandu. A prospective cohort study of gunshot injury was conducted during Septemeber 2015 and early outcome analysis was done.Results: There were thirteen cases of gunshot wound. The mean age of casualty was 31 years and all were male.Injury Number (n) Intervention Outcome Large intestine 3 Laparotomy Ileostomy/wound infection Perineum 1 Exploration/Debridement Loop ileostomy Bone- fracture 3 Traction/Enternal fixation/ Open reduction Internal Fixation Vertebra 1 Right hip power 3/5 Vascular- AV fistula 1 stenting Referred Brachial plexus 1 Sensory/motor deficit Neck 2 Exploration Marginal mandibular nerve palsy Elbow 1 Debridement/Split thickness skin graftThere were no mortality and one case was referred.Discussion: With the multidisciplinary team approach, all the gunshot injury cases were managed with good outcome. The timely intervention by a surgical team is an essential factor for proper management of gun shot injury.

MANAGEMENT OF CASE OF MASSIVE SPLENOMEGALY WITH GAUCHER’S DISEASE FOR SPLENECTOMY.

30Jun 2016 MANAGEMENT OF CASE OF MASSIVE SPLENOMEGALY WITH GAUCHER’S DISEASE FOR SPLENECTOMY. Savita Chauhan , Charuta Gadkari , Anjali Bhure and Sohal Parate. Assistant Lecturer, Dept of Anaesthesiology, NKP Salve Institute of Medical Sciences & Research Center, Nagpur-440019, Maharashtra, India. Associate Professor, Dept of Anaesthesiology, NKP Salve Institute of Medical Sciences & Research Center, Nagpur-440019, Maharashtra, India. Professor and HOD, Dept of Anaesthesiology, NKP Salve Institute of Medical Sciences & Research Center, Nagpur-440019, Maharashtra, India. Resident JR-III, Dept of Anaesthesiology, NKP Salve Institute of Medical Sciences & Research Center, Nagpur-440019, Maharashtra, India.

A prospective anatomic study of epipteric bones in dry human skulls of Karnataka

Aim: To study the incidence of epipteric bones in the region of pterion in human skulls.Materials & Methods: The study was done for a period of two years in the department of Anatomy at Kempegowda Institute of Medical Sciences and Research center, Bengaluru. A total of 100 dry unknown skulls from bone sets possessed by first year students of Kempegowda Institute of Medical Sciences and Research center, Bengaluru of 2009 2011 batch were evaluated for the presence of epipteric bones.Results: Out of 100 skulls, epipteric bones were seen in the region of pterion in 24 skulls. Bilateral occurrence was noted in 8 skulls and unilateral occurrence was seen in the remaining 16 skulls. Among the unilateral occurrence, 6 were seen on the right side and 10 were seen on the left side.Conclusion: In conclusion, identification of the occurrence of epipteric bones at the site of pterion is important as they may be confused for fractures. Also, presence of epipteric bones may lead to complications in making burr holes at the pterion.

Prevalence of depression amongst adolescents in rural area of South India – a school based cross sectional study

Introduction:Depression is common in adolescents and especially in high school students and is one of the most frequent mental disorders among teenagers. Depression in adolescents is an under recognized mental health problem because they remain indecisive to disclose their feelings and seldom seek psychiatric help. One of the factors that make depression so difficult to diagnose in adolescents is the common behavioural changes that are normally associated with the hormonal changes of this period. It has only been in recent years that the medical community has acknowledged childhood depression and viewed it as a condition which requires intervention. Materials and Methods:Aschool based cross-sectional study among adolescents was conducted by using self-administered structured “Patient Health Questionnaire-9 modified for Adolescents (PHQ-A) questionnaire” in rural field practice area of A.J.Instituteof Medical Sciences & Research Center, Mangaluru. Results:Total 284 students participated in the study and prevalence of depression was 49%. Prevalence of depression increased with age (p-value:0.001) and was higher among females (p-value:0.02). Discussion:Prevalence of depression in this study matched with other studies conducted in India. Majority of the students suffered from mild depression which indicates early diagnosis is crucial in prevention of progression to moderate and severe forms of depression. Conclusion:Prevalence of depression among adolescents is high. There is an urgent need for incorporation of simple screening methods for depression in school health program.

Interprofessional education: it’s happening!

Introduction: Integrating interprofessional education (IPE) into an academic medical sciences center is an innovating strategy that has been adopted by faulty at a medial sciences university. As home to six professional colleges charged with preparing students to become highly skilled healthcare professionals, faculty recognize the importance of an interprofessional approach to education. Methods: Two approaches include the use of the simulation center and a student-led community based clinic. In the simulation certer, nursing students visit a simulated home health patient with diabetes and present findings at a team conference where medicine, pharmacy, and nursing develop a plan of care. Afterward, the patient is seen by the students in an acute situation and debriefing follows the eperience. Another interprofessional experience involves student and preceptors at a free community-based clinic providing care for an underserved population. Students see patients as teams and present findings to an interprofessional team of preceptors. Results: IPE experiences bridge education and practice eliminating existing educational silos. Students gain confidence in the core interprofessional competencies: communication, ethics, roles, and teamwork with the goals of improving quality patient outcomes. Students are prepared to embrace a new paradigm of healthcare delivery. Discussion: The major challenge for involving student across professional is the coordination of course schedules. There must be share conviction that valuable input from each profession developing a plan of care is important to obtaining better patient outcomes. Preceptors also learn from other educators participating, contributing to a true collegial atmosphere and role modeling for our future health care professionals.

Clinical Study on Risk Factors of Pelvic Organ Prolapse in Reproductive Age Group and Study of Abdominal Sling Surgeries done for It, with respect to Early Complications, Anatomical Results, and Symptoms of Descent

ABSTRACT Introduction The purpose of a conservative procedure is the correction and restoration of the prolapse with the most effective long-lasting result, which allows sexual functioning and, for young patients, conservation of reproduction. Objectives To study the risk factors for prolapse in reproductive age group; to study various conservative surgeries done for pelvic organ prolapse (POP). Materials and methods This is a retrospective study conducted on patients presenting with pelvic organ prolapse in reproductive age group, at Vydehi Institute of Medical Sciences and Research Center, from June 2008 to June 2014. Results We identified 68 women with uterovaginal prolapse in reproductive age group who underwent conservative surgeries: 26 (18%) underwent Virkud's surgery, 20 (29.4%) underwent Shirodkar's sling surgery, and 22 (32.3%) underwent Purandare's sling surgery. There was no major difference in intraoperative and postoperative details in all three surgeries. Postoperative improvement in symptoms was 16 (80%) in Shirodkar's group, 20 (90%) in Virkud's composite sling surgery, and 16 (61.5%) in anterior abdominal wall cervicopexy surgery. Conclusion Obstetric risk factors are the major contributing factors for pelvic organ prolapse in the reproductive age group. Proper intranatal care will help in reducing the problem; all the conservative surgeries have similar intraoperative, postoperative morbidity and are similar with respect to early anatomical correction. Urinary tract infection and urinary retention were more in anterior abdominal wall hysteropexy group; Patient's satisfaction and success rate is more in Sacropexy and Virkud's composite surgery group compared to anterior abdominal wall hysteropexy group. How to cite this article Lakshmidevi M, Venkatesh S, Sheela HS. Clinical Study on Risk Factors of Pelvic Organ Prola in Reproductive Age Group and Study of Abdominal Sling Surgeries done for It, with respect to Early Complications, Anatomical Results, and Symptoms of Descent. J South Asian Feder Obst Gynae 2016;8(3):202-206.

Chiba study of Mother and Children's Health (C-MACH): cohort study with omics analyses

PurposeRecent epidemiological studies have shown that environmental factors during the fetal period to early childhood might affect the risk of non-communicable diseases in adulthood. This is referred to as the...

Study of clinical profile of stroke patients in rural tertiary health care centre

Background: There are 15 million people worldwide who suffer a stroke each year. According to the World Health Organization, stroke is the second leading cause of death for people above the age of 60 years, and the fifth leading cause in people aged 15 to 59 years old. Each year, nearly six million people worldwide die from stroke. One in six people worldwide will have a stroke in their lifetime. Methods: This cross sectional study was carried out from July 2014 to April 2016 of all new patients admitted with stroke in Indian institute of Medical Sciences and Research Center, Badnapur, Jalna, Maharashtra. India. Results: The cerebrovascular strokes were more common in males (58.53%) than females (41.46%). Most common age group was 61-70 years (34.95%). The Most common clinical feature was Hemiplegia (55.28%). most common risk factor was hypertension (48.78%), tobacco chewing (26.01%), smoking (19.51%), followed by past h/o of cerebrovascular stroke (12.19%), Dyslipidemia (8.94%). Most common type of stroke was ischemic (68.28%) and hemorrhagic (31.69%) was second most common in ischemic strokes most common involved areas were parietal (30.08%), basal ganglia (9.75%), frontal lobe (7.31%). In hemorrhagic stroke most common site was thalamus (10.56%) followed by ventricular (5.69%) and basal ganglia (4.06%). Conclusions: Young patients (age ≤45 years) were 16.26% which is more dangerous in view of productive year lost. Stroke having male predominance with hypertension was the most common risk factor smoking tobacco chewing and dyslipidemia were other risk factors for stroke and most common type of stroke was ischemic. Developing countries like India are facing a double burden of communicable and non-communicable diseases. Stroke is one of the leading causes of death and disability in India.

Effect of dengue fever on serum aminotransferases in children

Background: Dengue infection is associated with liver dysfunction, which tends to become severe with severe dengue. In the acute phase of the disease, an increase occurs in aminotransferases, the levels of which subsequently decrease as the liver function recovers. Significant rise of liver enzymes helps in recognition of severe forms of dengue infection.Methods: The study was conducted in the Department of Pediatrics of Vydehi Institute of Medical Sciences and Research Center, Bangalore from January 2015 to June 2015. 50 patients of 1-14 years age group, who presented with fever of recent duration and serologically positive for dengue, were included in the study.Results: 38% (19) were females and 62% (31) were males. NS1 was positive in 48% (24) and IgM was positive in 52% (26) of patients. IgG was negative in all the patients. AST was raised in 36% (18) of patients, ALT was raised in 34% (17) patients and ALP raised in 42% (21) patients. AST (Mean ± SD 53.84±43.32) was raised more than ALT (Mean ± SD 48.76±41.11)Conclusions: Liver enzymes were mildly elevated in patients with dengue fever in the initial stage of illness. AST was elevated more than ALT.

Explanatory Meetings on Thyroid Examination for the "Fukushima Health Management Survey" after the Great East Japan Earthquake: Reduction of Anxiety and Improvement of Comprehension.

After the Fukushima Daiichi nuclear accident in 2011 and thyroid examinations of children in Fukushima, the Radiation Medical Science Center began "Explanatory meetings on thyroid examination" as a method of communication with residents such as the subjects themselves and their guardians. Through questionnaires, we examined the relationship between anxiety (regarding the effects of radiation on the thyroid) before the meetings and individual attributes including attitudes on radiation, and then verified the effects of the meetings using measures of anxiety, comprehension, and satisfaction, as the outcomes. Of the meetings in 2014-2015, 799 people attended 30 sessions in Kenchu, Kenpoku, Iwaki, Soso, and outside of Fukushima Prefecture, and 594 people responded the questionnaires before and after the meetings on the same day. Level of anxiety before the meetings varied depending on individual attributes (including attitudes regarding collection information on radiation, advisors on radiation, and levels of subjective understanding), highlighting the importance of presenting information about radiation in a manner that is easy to understand, as well as providing opportunities for the exchange of opinions. Participation in meetings reduced anxiety. This was largely attributed to explanations about general characteristics of cancer and objective facts, including doses; status of the Chernobyl accident; and comparison in results of thyroid examinations with other prefectures in Japan. An opportunity for a question-and-answer session also contributed to increased overall satisfaction. The lower number of meeting participants was associated with anxiety reduction and higher subjective comprehension. The present findings obtained will be useful to facilitate evidence-based risk communication.

American Society for Metabolic and Bariatric Surgery position statement on intragastric balloon therapy endorsed by the Society of American Gastrointestinal and Endoscopic Surgeons

American Society for Metabolic and Bariatric Surgery position statement on intragastric balloon therapy endorsed by the Society of American Gastrointestinal and Endoscopic Surgeons Mohamed R. Ali, M.D.*, Fady Moustarah, M.D., Julie J. Kim, M.D., on behalf of the American Society for Metabolic and Bariatric Surgery Clinical Issues Committee Department of Surgery, University of California-Davis Medical Center, Sacramento, California Faculty of Medical Sciences & Neuroscience Research Center, Lebanese University, Beirut, Lebanon Department of Surgery, Central Michigan University College of Medicine, Mount Pleasant, Michigan Department of Surgery, Tufts University School of Medicine, Boston, Massachusetts Received December 16, 2015; accepted December 18, 2015

Assessment of 1P/19Q Deletionby Flourescenceinsitu Hybridization (FISH) in Glioma Patientsfrom Asingle Institute Experience

The discovery of 1p and 19q chromosomal arms deletion in glial tumors influences both more objective diagnosis and more accurate prediction of chemotherapy response. As a result an attempt has been made to detect deletion using fluorescence in-situ hybridization (FISH) and analyzed its prognostic value in a cohort of glial tumor patients from Amrita Institute of Medical Sciences and Research Center Kochi. FISH was performed on 66 FFPE tissue sections by using Vyis LSI 1p36/LSI 1q25 and LSI 19p13/LSI 19q13dual coloured FISH probe sets. Signals were scored from at least 150-250 non-overlapping, intact nuclei. 163 cases were analyzed. Both 1p and 19q deletions was observed only in 28/163 (17.17%), - 1p/+ 19q deletion 80/163 (49.07%) and+1p/-19q deletion 55/163(33.74%) . In this work presented the FISH was successfully applied to identify deletion 1p/19q. Its incidence depends on the type of diagnosed gliomas. In contrast to reported data, the present study reveals 49.07% deletion - 1p/ + 19q. Deletions also have prognostic significance in the test group what constitutes the basis for inclusion of determining deletion 1 p/19q into diagnostic and treatment algorithm.