Glioblastoma multiforme (GBM) is a highly aggressive and poor prognosis brain tumor with about 12- to 18-month median survival period and high mortality rate in human. Glioma cells are able to disseminate and migrate far away from the primary tumor and display treatment resistance. Therefore, upgrading our knowledge regarding the genetic aspects of GBM and also applying a safe and promising source of therapeutic agent to prevent GBM might bring some new hopes to treat this disease. TWIST1 and FOXM1 upregulations are associated with migration and metastasis in gliomas. 3-Acetyl-11-keto-β-boswellic acid (AKBA) has been shown to inhibit the growth of a wide variety of cancer cells, such as gliomas. The aim of this study was to investigate the possible inhibitory effects of AKBA on the TWIST1 and FOXM1 gene expression levels and the migration of glioblastoma cancer cells. U87MG cells were treated with or without 50 µM of AKBA. The expression levels of TWIST1 and FOXM1 were assessed using real-time PCR. The migration of cells in the presence or absence of AKBA was examined through the wound healing assay. AKBA significantly downregulated both TWIST1 and FOXM1 gene expression levels. The migration of U87MG cancer cells was significantly declined when AKBA was applied. AKBA potentially prevents FOXM1 and TWIST1 axis in glioblastoma cancer cells and also inhibits cell migration.