Media Optimization Studies Research Articles

Media optimization studies for glucose isomerase production by arthrobacter species

Media optimization studies are carried out with the objective of maximising glucose isomerase production by Arthrobacter sp. The recommended media consists of 1.0% (w/v) xylose, 1.0% peptone, 0.5% yeast extract, 0.025% MgSO4·7H2O, 0.6% (NH4)2HPO4 and 0.2% KH2PO4. Activity of the enzyme produced in this media is 11.2 units/ml. Growth cycle for batch cultivation is studied and the lag period is 2 hours, followed by exponential phase extending upto 32 hours.