<p>The Regenerated of Siam Tangerin through Somatic<br />Embryogenesis. Ali Husni, Agus Purwito, Ika Mariska,<br />and Sudarsono. Somatic embryogenesis occurs in most<br />plants that are cultured on a suitable medium in vitro.<br />Somatic embryo may arise from single cells and the embryogenic<br />cells are widely applicable in plant propagation,<br />genetic manipulation and transgenic technologies. The<br />present study was carried out to develop an effective<br />somatic embryogenesis technique to regenerate Siam<br />tangerine plants. Materials used in this study were nucellar<br />tissues of young fruits (30-90 days post anthesis). Induction<br />of embryogenic calli was done by culturing the tissues on<br />three different basal media (MS, MW and MT). Embryo<br />maturation was done on the MW medium + ABA (0; 0.1; 0.3;<br />dan 0.5 mg/l), while germination to plantlet development<br />was done on WS medium + GA3 (0, 0.1, 0.3, and 0.5 mg/l).<br />The results showed that among the three media, MW was<br />the best medium for callus induction from nucellar tissues<br />with a success rates 98% for Simadu and 100% for<br />Pontianak. The best maturation of embryo somatic was<br />done on MW medium + ABA 0.5 mg/l with success rates<br />99%, while the best medium for germination and development<br />into planlets was MW medium + 0.5 mg/l GA3 with a<br />success rate 58%.</p>