The integration of two-dimensional (2D) nanochannels with differing properties presents a promising avenue for mimicking ion transport processes in biological systems. In this study, a bioinspired Janus nanochannel membrane (BJNM) is developed by incorporating intercalated montmorillonite (MMT) and graphene oxide (GO) nanosheets. The asymmetric characteristics in chemical composition, structure, and charge distribution confer the BJNM with the ability to rectify ion transport, as evidenced by nonlinear current-voltage relationships. Through optimization of the electrolyte solution and applied voltage range, a notable rectification ratio of 40.9 is achieved. The underlying mechanism of ion rectification is explored through numerical simulations, highlighting the significance of 2D heterojunctions. These findings provide valuable insights for the design of nanofluidic systems tailored for precise ion transport, biosensing applications, and energy conversion processes.