Mechanism Of Occurrence Research Articles

Study on the mechanism and prevention system of creep induced instability of isolated coal pillars considering time effect

In response to the frequent occurrence of impact events in isolated coal pillars between the upper (lower) mountain areas of mining areas under conditions without obvious mining disturbance, this study takes multiple isolated coal pillar impact events that occurred in Zhaolou Coal Mine and Gengcun Coal Mine as examples. It conducts a mechanism study on the creep-induced impact ground pressure of isolated coal pillars considering the time effect. The occurrence of such impact ground pressure accidents often exhibits significant “lag” and “spontaneity”. Due to the unclear mechanism of occurrence and the difficulty in grasping the unloading timing, this type of impact ground pressure has become a typical hidden disaster, posing a serious threat to the deep mining of coal mines. Based on the analysis of the creep mechanical properties of coal and rock mass, the uniaxial compression acoustic emission creep test of coal body, and the FLAC3D numerical simulation, a creep instability impact mechanical model of coal and rock mass is established. This model reveals the mechanism of creep instability impact and the stress evolution law of coal and rock mass during creep. The mechanical and energy criteria for the occurrence of impact ground pressure in isolated coal pillars under the action of unstable creep are proposed. The study shows that the action of high ground stress is a necessary condition for the occurrence of "lag-type" impact ground pressure in isolated coal pillars. Long-term unstable creep will weaken the support strength of the entry. When the elastic energy accumulated in the isolated coal pillar exceeds its ultimate bearing capacity, impact instability will occur. Based on the mechanism of occurrence of such impact accidents, targeted deep hole blasting unloading measures are proposed, providing a good reference value for the prevention and control of "lag-type" impact ground pressure accidents in isolated coal pillars.

“Perplexing” cough

Cough is a serious problem of practical pulmonology. The mechanisms of cough occurrence are diverse and numerous diseases are associated with chronic cough syndrome. The diagnosis can be challenging because of a “perplexing”, “unexplained”, or “idiopathic” cough. The aim of the review was to consider the main underlying mechanisms of the “perplexing” cough, its potential course, an algorithm for managing patients with the persistent cough, and new treatment methods. Conclusion. Chronic “perplexing” cough disrupts the patient’s quality of life and reduces their performance. Unveiling the mechanisms of its development may increase the treatment efficacy.

Particle size characteristics of sliding-zone soil and its role in landslide occurrence: a case study of the Lanniqing landslide in Southwest China

In landslide studies, particle size is a key quantitative indicator, reflecting the formation and development of the sliding zone. It plays a crucial role in understanding the mechanisms and evolutionary processes that lead to landslide occurrences. Precise measurement of particle size is crucial. This study centered on soil samples from the Lanniqing landslide in Southwest China. To begin, seven distinct methods were used to preprocess the soil samples. Next, the particle size frequency distribution was measured using the Mastersizer 2000 laser particle size analyzer. Key parameters, including median particle size, mean particle size, sorting coefficient, skewness, and kurtosis, were then compared and analyzed to determine the most appropriate preprocessing method for evaluating the characteristics of the soil samples. The mechanism of landslide occurrence was subsequently analyzed by examining the particle size characteristics, mechanical properties, and mineral composition of the soil samples. The results suggested that method C provides the most reliable analysis of particle size characteristics in soil samples. The observed coarsening of coarse particles, along with a significant increase in clay content within the sliding zone, indicates that the sliding surface has undergone multiple shear and compression events. The interplay of the upper traffic load and slope cutting at the front edge set the stage for the Lanniqing landslide, prompting the initial development of potential sliding surfaces. Rainfall acts as a catalyst for slope instability. The high clay content, combined with the formation of a low-permeability layer rich in clay minerals on the sliding surface, leads to excessive pore water pressure and mineral lubrication. These factors inherently trigger and accelerate the occurrence of the landslide.

Significant Association Between Arctic Oscillation and Winter Wildfires in Southern China

AbstractThe recent increase of regional wildfire occurrences has been associated with climate change. In this study, we investigated the association between the February to March wildfire points and burned area in the southern region of China (20°N–30°N and 105°E–115°E) and the simultaneous Arctic Oscillation (AO) index during 2001–2022 and 2001–2020, respectively. After removing the El Niño-Southern Oscillation and Indian Ocean Dipole signals, time series of the regional mean fire points and burned area over the study area is significantly correlated with the AO index at − 0.37 and − 0.47, significant at the 0.1 level. Precipitation significantly affects wildfire variations. The positive AO could trigger a southeastward Rossby wave train and induce anomalous cyclone activity approximately located in the area encompassed by 15°N–27°N and 85°E–100°E. This outcome could help to enhance the southern branch trough and results in positive precipitation anomalies in southern China. This increasing moisture is conductive to reducing wildfire risks, vice versa. Our results are potentially useful for strengthening the understanding of the mechanisms of wildfire occurrences in southern China.

Assessment of Physical Processes for Describing Mechanism of Occurrence and Measurement of Consciousness

Currently, there is a growing number of proposed physical processes for describing the mechanism underlying the occurrence and measurement of consciousness. These bodies of knowledge have created multitudes of viewpoints about consciousness. It is also still unclear how they are related or whether they can be empirically distinguished. This study presents an assessment of physical processes for describing the occurrence and measurement of consciousness based on (1) the von Neumann–Wigner approach, (2) orchestrated objective reduction, (3) integrated information, (4) consciousness as a state of matter, and (5) electromagnetic field. The study underscores the significance, similarity, and weakness of these approaches. All approaches except the von Neumann–Wigner approach agree on the fact that the brain is the central region responsible for generating and detecting consciousness but with distinct explanations. Further, both surveyed approaches are still far from experimental verification. Further studies are needed on designing experimental verification and proposing new approaches.

Contemporary issues of the birth trauma

Background. Birth trauma is a group of diseases caused by mechanical factors during childbirth. Birth trauma is an interdisciplinary problem, affecting various fields of medicine, such as obstetrics, pediatrics, child neurology and other sciences. Birth trauma occupies an important place in the structure of perinatal morbidity and mortality. Birth injuries of newborns during vaginal birth occur in 3.6% of cases, during cesarean section — in 1.2%. However, at present, despite a comprehensive study of the problem, the incidence of birth trauma in newborns remains high. Along with obstetric birth trauma, spontaneous birth trauma also occurs — not associated with any obstetric care.Purpose of review. To summarize and analyze the available data on the causes of birth trauma in newborns depending on the type of birth injury.Materials and methods. The review includes published data over the past 10 years regarding the study of the causes of the main types of birth injuries in newborns. The literature search was conducted in Web of Science, Google Scholar, PubMed, and ELibrary databases.Results. The problem of fetal-pelvic disproportion in the genesis of the development of risk factors for maternal-fetal trauma remains relevant. Fetal macrosomia is one of the most significant factors in birth trauma. The most common skull injury in newborns is cephalohematoma. According to the mechanism of occurrence, birth trauma is divided into spontaneous — occurring during physiological childbirth — and obstetric — associated with any obstetric benefits. The degree of configuration of the fetal head plays a significant role in the pathogenesis of birth trauma. Birth trauma is considered as a systemic reaction on the part of the newborn’s body, which leads to a breakdown of compensatory and adaptive mechanisms and the development of critical damage to the central nervous system and is differentiated from birth damage, which includes only local changes.Conclusion. To search for preventive measures aimed at preventing birth injuries in newborns, it is necessary to systematize the groups of causes that influence the increased risk of birth injury. Timely assessment of the pathological configuration of the fetal head is extremely important when deciding on the advisability of using obstetric aids during vaginal operative delivery, since it is an early predictor of the formation of fetal-pelvic disproportion — a significant factor in birth trauma in the newborn. In recent years, taking into account the active study of molecular genetic mechanisms in the genesis of the formation of various pathological conditions, the search for various genetic and epigenetic factors that influence the risk of developing birth traumatic injuries in a newborn against the background of individual susceptibility to the effects of any physical factors during childbirth.

Silver nanoparticles regulate antibiotic resistance genes by shifting bacterial community and generating anti-silver genes in estuarine biofilms

Biofilms are thought to be sinks for antibiotic resistance genes (ARGs) and nanoparticles (NPs), however, studies on the interactions between NPs and ARGs in biofilms are limited. This study focused on the occurrence and regulatory mechanisms of ARGs during the formation of biofilms with continuous treatment of zero-valent silver nanoparticles (Ag0-NPs) and Ag ions at an environmental concentration of 10 µg/L in the Yangtze Estuary. The biofilms could enrich large amounts of Ag, with the highest concentration of 97.60 mg/kg and 111.08 mg/kg in the Ag0-NPs and Ag ions group at 28 days. Compared to the blank at 28 days, the abundance of ARGs was reduced 2.2 times in the Ag0-NPs group, whereas it increased 1.3 times in the Ag ion group. Ag0-NPs and Ag ions induced the production of silver resistance genes (SRGs) or selected bacteria with SRGs in biofilms. Based on machine learning, the bacterial community, SRGs, and Ag concentration were the top three dominant regulators of ARGs, with 27.74 %, 25.57 %, and 17.93 % contributions, respectively. Structural equation modeling revealed that Ag could indirectly regulate ARGs by regulating the bacterial community in the Ag0-NPs group. Metagenomic sequencing further showed that most of the decreased ARGs were hosted by Betaproteobacteria in the Ag0-NPs groups. According to the KEGG pathway database, the possible molecular mechanism of Ag0-NPs/Ag ions regulating ARGs may be through the two-component system (arlS/silS-arlR) and beta-lactam resistance system (mexI-mexV-oprM/oprZ/smeF). Overall, this study provides new insights into the effects of Ag0-NPs at environmental concentrations on the ecological environment, especially regarding the mechanism of regulating ARGs in estuarine biofilms.

A co-evolutionary approach to MNE-assisted industry development: Uruguay’s forest-based pulp sector

Purpose This study aims to investigate how co-evolutionary interactions between multinational enterprises (MNEs) and local actors shaped resource-intensive industry development over several decades, examining the economic, industrial and social implications of these interactions. It details the long-term effects of MNEs’ activities on the host country’s economic and social development and unfolds the path-dependent sequence and mechanisms of occurrence. Design/methodology/approach In a historical case study of Uruguay’s forest-based pulp sector during the 1970s to 2023, the authors analyze data from interviews with local industry actors and from company archives, academic publications and public environmental organizations. Findings The findings untangle the path-dependent co-evolution between MNEs’ activities and local actors and the resulting gradual development of the local industry. The increasing commitment of MNEs to the host country and their engagement with business and social communities impact the development of the industry’s technology basis, activity networks, identity and market. Spillovers and linkages occur in these interactions, driving industry development processes forward. The authors also reveal how MNEs attempt to address the social and environmental tensions associated with their operations to support their long-term presence in the host country. Research limitations/implications The authors contribute to the MNE-assisted development literature by untangling the specific mechanisms of MNEs’ engagement in local industry development over a long-term period and elaborating on its industrial and social implications. The authors enhance knowledge about spillovers by capturing a wide range of spillover and linkage effects and assessing their long-term impacts on industry development. Practical implications The authors offer insights into how policymakers can tailor instruments for attracting MNEs by adopting a long-term perspective regarding the implications for local development and accounting for economic, industrial and social impacts. This approach can maximize beneficial outcomes from MNEs’ presence and limit tensions between MNEs and local actors. Social implications This historical case study illustrates the complexity of MNEs’ engagement with local actors over time, including various tensions and positive developments. It illustrates the importance of social acceptance in achieving quality linkages between local and foreign actors. Originality/value This historical analysis untangles the path dependency in the long-term impacts of MNE-assisted development, illustrating the value of a temporal perspective.

Correlations Between Spatiotemporal Variations in Phytoplankton Community Structure and Physicochemical Parameters in the Seungchon and Juksan Weirs

The Yeongsan River is one of the four major rivers in South Korea. Since the construction of two weirs as part of the Four Major Rivers Project to secure water resources in 2011, issues with algal blooms have frequently arisen, prompting the Ministry of Environment of Korea to conduct continuous monitoring of water quality and algal outbreaks. This study, conducted between 2019 and 2023, examined the relationship between the phytoplankton community structure and physicochemical factors at the Seungchon and Juksan weirs. Phytoplankton were categorized into four groups (Bacillariophyceae, Chlorophyceae, Cyanophyceae, and other phytoplankton), and 20 dominant genera were selected for analysis. As microalgal species vary depending on environmental conditions, understanding the specific relationships among the microalgae observed in the study area can help explain their occurrence mechanisms and contribute to the development of effective management strategies. Therefore, we used principal component analysis (PCA) to analyze the seasonal variation patterns of the four microalgal groups and visualize key data features through dimensionality reduction. Additionally, PCA was employed to identify and visualize environmental factors related to seasonal variations in phytoplankton communities. PCA helped elucidate how different environmental factors influence phytoplankton fluctuations across seasons. We used canonical correspondence analysis (CCA) to investigate the relationships among the 20 dominant genera in each group and environmental factors. Additionally, CCA was used to analyze the relationship between the distribution of the top five dominant phytoplankton taxa in each group and various environmental factors. CCA allowed for a detailed examination of how these dominant taxa interact with environmental conditions. PCA revealed significant correlations between other phytoplankton and Chl-a in spring and Cyanophyceae and water temperature in summer. Bacillariophyceae was positively correlated with nitrogen-based nutrients but negatively with phosphate phosphorus (PO4-P). CCA revealed significant correlations between dominant genera and environmental factors. Stephanodiscus sp. was associated with nitrogen-based nutrients, whereas Microcystis sp. and Dolichospermum sp. were associated with water temperature and PO4-P. Stephanodiscus sp. affected water treatment through filtration and sedimentation issues, whereas Microcystis sp. and Dolichospermum sp. produced the toxin microcystin. These findings offer valuable insights for water quality management.

Step-by-step silicon carbide graphitisation process study in terms of time and temperature parameters

This work investigates the temperature and time as key parameters for graphene formation on the silicon carbide surface during the high thermal decomposition process. Measurements were performed using various experimental techniques under ultra-high vacuum conditions. The graphitisation process was divided into various stages, after which the surface chemical composition and atomic structures were analysed in detail. It has been shown that despite the known theory of graphitisation mechanism and initial condition for occurrence of this process, the application of different temperatures and heating times affect the quality and quantity of formed graphene layers. Applying a temperature too low or annealing the sample for a too short time led to an inefficient silicon sublimation process. On the other hand, too high temperature during flashing modifies the visibility of surface structures, which may be crucial for other investigations and potential applications of such systems.

Ferroptosis Induction Enhances Photodynamic Therapy Efficacy for OLK.

Oral leukoplakia (OLK) is the most representative oral potentially malignant disorder, with a high risk of malignant transformation and unclear mechanisms of occurrence. Recently, photodynamic therapy (PDT) has exhibited great potential in the treatment of OLK. However, the efficacy of PDT is difficult to predict and varies from person to person. Ferroptosis-related pathways are upregulated in many cancers, and ferroptosis induction is considered to be a potential synergistic strategy for various antitumor therapies, but its role in OLK treatment remains unclear. This study aimed to determine whether ferroptosis induction can enhance the efficacy of PDT in OLK treatment. Our study revealed that solute carrier family 7 member 11 (SLC7A11), a component of a crucial amino acid transporter and a key negative regulator of ferroptosis, was found to be highly expressed in OLK patients with no response to PDT. 5-Aminolevulinic acid (ALA)-PDT is known to cause apoptosis and necrosis, but ferroptosis also occurred under ALA-PDT in OLK cells in our study. Using erastin to induce ferroptosis enhanced the efficacy of ALA-PDT on OLK cells by disrupting the antioxidant system and further elevating intracellular reactive oxygen species levels, leading to increased apoptosis. Furthermore, this combined modality also enhanced the efficacy of ALA-PDT on 4-nitroquinoline-1-oxide (4NQO)-induced OLK lesions in mice. In summary, ferroptosis induction may serve as a potential strategy to enhance the efficacy of ALA-PDT for OLK treatment.

Prospects of vaccination against pneumococcal infection based on the asthma phenotype

According to recent studies, bronchial asthma is characterized by a wide variability of the mechanisms of occurrence and progression. This heterogeneity is caused by patterns of predominant cells and inflammatory mediators, which determine differences in immunological parameters observed in patients with certain endotypes depending on the dominant type of mediators (high and low T2 inflammation). In long-term observations, a substantial research base has been accumulated justifying the effectiveness of vaccination against pneumococcal infection in patients with asthma. The vaccination decreases the frequency of exacerbations of the disease and hospitalizations in the short and medium term. However, these studies evaluated the asthma patients as a wholesome population, and it remains unexplored whether the effect of pneumococcal vaccines on asthma differs depending on the endotype of the disease and what are the mechanisms of such a differentiated effect.The aim of this work is to present the results of recent quality studies on changes in the profile of inflammatory asthma mediators under the action of immunobiological substances based on Streptococcus pneumoniae antigens, primarily from the vaccines.Conclusion. The asthma heterogeneity can lead to different clinical outcomes in pneumococcal infection and, respectively, the clinical effects of immunization in patients differentiate according to the nature of inflammation. In other words, the uniformity of the clinical effect of vaccination against pneumococcal infection in all patients in ongoing studies may represent the combined effect of molecular mechanisms regulating the specific activity of Th1-, Th2-, Th17-, NKT-, and Treg-cells. The results of studies proving the ability of pneumococcal vaccines to modulate the Th1-, Th2-, Th17-, Treg immune response in patients with asthma contributed to increased interest in developing new immunoregulatory therapeutic agents based on S. pneumoniae antigens.

Advances in macrophage-myofibroblast transformation in fibrotic diseases.

Macrophage-myofibroblast transformation (MMT) has emerged as a discovery in the field of fibrotic disease research. MMT is the process by which macrophages differentiate into myofibroblasts, leading to organ fibrosis following organ damage and playing an important role in fibrosis formation and progression. Recently, many new advances have been made in studying the mechanisms of MMT occurrence in fibrotic diseases. This article reviews some critical recent findings on MMT, including the origin of MMT in myofibroblasts, the specific mechanisms by which MMT develops, and the mechanisms and effects of MMT in the kidneys, lungs, heart, retina, and other fibrosis. By summarizing the latest research related to MMT, this paper provides a theoretical basis for elucidating the mechanisms of fibrosis in various organs and developing effective therapeutic targets for fibrotic diseases.

When does newcomer get feedback? Relationship between supervisor perceived organizational support and supervisor developmental feedback

PurposeUsing social exchange theory, this paper explores the relationship between supervisor perceived organizational support and supervisor developmental feedback. It verifies the mediating role of supervisor felt obligation to organization and the moderating role of supervisor core self-evaluation in this relationship.Design/methodology/approachA two-wave survey sampling method was applied to collect 247 pieces of paired data (supervisors-new employees) in organizations in China.FindingsThe results show that supervisor felt obligation to organization mediates the relationship between supervisor perceived organizational support and supervisor developmental feedback. The findings suggest that a high level of supervisor core self-evaluation strengthens the relationship between supervisor perceived organizational support and supervisor felt obligation to organization and then facilitates supervisor developmental feedback.Originality/valueThis study combines two perspectives, organizational factor and supervisor trait, to reveal the occurrence mechanism of supervisor developmental feedback and complements the research on the influencing factors of supervisor developmental feedback. In addition, it provides some theoretical guidance and practical insights into how organizations can motivate supervisors to provide developmental feedback to new employees.

Evaluation of European Watch List Contaminants in Environmental Matrices and Microplastics: Analytical Strategies, Mechanisms of Adsorption and Occurrence

Evaluation of European Watch List Contaminants in Environmental Matrices and Microplastics: Analytical Strategies, Mechanisms of Adsorption and Occurrence

Advances in the Effects of Heat Stress on Ovarian Granulosa Cells: Unveiling Novel Ferroptosis Pathways.

Heat stress has been one of the key research areas for researchers due to the wide-ranging effects and complex mechanisms of action of its stress product reactive oxygen species (ROS). The aim of this paper is to comprehensively review and summarize the effects of heat stress on ovarian granulosa cells and their mechanism of action. We systematically reviewed the effects of heat stress on ovarian granulosa cells, including intracellular steroid hormone changes, oxidative stress, apoptosis, and mitochondrial function. Meanwhile, this paper discusses in detail several major mechanisms by which heat stress induces apoptosis in ovarian granulosa cells, such as through the activation of apoptosis-related genes, induction of endoplasmic reticulum stress, and the mitochondrial pathway. In addition, we analyzed the mechanism of ferroptosis in ovarian granulosa cells under heat stress conditions, summarized the potential association between heat stress and ferroptosis in light of the existing literature, and explored the key factors in the mechanism of action of heat stress, such as the signaling pathways of Nrf2/Keap1, HSPs, and JNK, and analyzed their possible roles in the process of ferroptosis. Finally, this paper provides an outlook on the future research direction, describing the possible interaction between heat stress and ferroptosis, with a view to providing a theoretical basis for further understanding and revealing the complex mechanism of ferroptosis occurrence in ovarian granulosa cells under heat stress.

Occurrence and enrichment mechanism of uranium in the Yankong phosphorite-type uranium deposit, Northern Guizhou

Occurrence and enrichment mechanism of uranium in the Yankong phosphorite-type uranium deposit, Northern Guizhou

Experimental investigation on the oil occurrence and mobility of lacustrine shales in offshore area of China using 1D/2D nuclear magnetic resonance and centrifugal techniques

Shale oil occurrence mechanism and mobility have significant influences on hydrocarbons extraction from shale systems. However, the effects of petrophysical and petroleum geochemical properties on oil mobility are not understood. In this study, shales with different lithofacies were investigated using thin sections, scanning electron microscopy, nuclear magnetic resonance (NMR), and organic geochemical analysis. Shales at original, extracted, oil-saturated, and centrifugal states were systematically scanned using 1D and 2D NMR technologies. The multi-scaled pores were obtained by comparing the T2 spectra of samples at different states. The 2D NMR identification spectra for hydrocarbon-bearing components were established. The free oil, adsorbed oil, mobile oil contents were quantitatively evaluated and the geological controls on oil mobility were discussed.Results show that the siliceous shales have the highest total oil and movable oil contents, while the argillaceous shales have the lowest values due to the limited pore space and poor connectivity. Mobile oil mainly stores in interparticle pores, dissolution-related pores and bedding fractures. Its content increases with the increasing felsic minerals. Oil in siliceous shales are rich in low carbon-chain hydrocarbons and has high mobility. Based on oil distribution in multi-scaled pores, a strategy about the mobile oil distribution were proposed: Ultramicropores region is the immobile oil zone; Micropores region is the oil difficult-to-flow zone; Transition pores region is the oil easy-to-flow zone; Macropores region is the completely mobile oil zone. The mobile oil mainly accommodates in transition pores and macropores (pore size >20 nm and mobile oil saturation exceeds 60 %).

Functional Bidirectionality of ERV-Derived Long Non-Coding RNAs in Humans.

Human endogenous retroviruses (HERVs) are widely recognized as the result of exogenous retroviruses infecting the ancestral germline, stabilizing integration and vertical transmission during human genetic evolution. To date, endogenous retroviruses (ERVs) appear to have been selected for human physiological functions with the loss of retrotransposable capabilities. ERV elements were previously regarded as junk DNA for a long time. Since then, the aberrant activation and expression of ERVs have been observed in the development of many kinds of human diseases, and their role has been explored in a variety of human disorders such as cancer. The results show that specific ERV elements play respective crucial roles. Among them, long non-coding RNAs (lncRNAs) transcribed from specific long-terminal repeat regions of ERVs are often key factors. lncRNAs are over 200 nucleotides in size and typically bind to DNA, RNA, and proteins to perform biological functions. Dysregulated lncRNAs have been implicated in a variety of diseases. In particular, studies have shown that the aberrant expression of some ERV-derived lncRNAs has a tumor-suppressive or oncogenic effect, displaying significant functional bidirectionality. Therefore, theses lncRNAs have a promising future as novel biomarkers and therapeutic targets to explore the concise relationship between ERVs and cancers. In this review, we first summarize the role of ERV-derived lncRNAs in physiological regulation, mainly including immunomodulation, the maintenance of pluripotency, and erythropoiesis. In addition, pathological regulation examples of their aberrant activation and expression leading to carcinogenesis are highlighted, and specific mechanisms of occurrence are discussed.

Exploring the Genetic Basis of Calonectria spp. Resistance in Eucalypts.

Selecting high-quality varieties with disease resistance by artificial crossbreeding is the most fundamental way to address the damage caused by Calonectria spp. in eucalypt plantations. However, understanding the mechanism of disease-resistant heterosis occurrence in eucalypts is crucial for successful crossbreeding. Two eucalypt hybrids, the susceptible EC333 (H1522 × unknown) and the resistant EC338 (W1767 × P9060), were screened through infection with Calonectria isolates, a pathogen that causes eucalypt leaf blight. RNA-Seq was performed on the susceptible hybrid, the disease-resistant hybrid, and their parents. The gene differential expression analysis showed that there were 3912 differentially expressed genes between EC333 and EC338, with 1631 up-regulated and 2281 down-regulated genes. The expression trends of the differential gene sets in P9060 and EC338 were similar. However, the expression trend of W1767 was opposite that of EC338. The similarity of the expression and the advantage of stress resistance in E. pellita suggested that genes with significant differences in expression likely relate to disease resistance. A GSEA based on GO annotations revealed that the carbohydrate binding pathway genes were differentially expressed between EC338 and EC333. The gene pathways that were differentially expressed between EC338 and EC333 revealed by the GSEA based on KEGG annotations were the sesquiterpenoid and triterpenoid biosynthesis pathways. The alternative splicing analysis demonstrated that an AS event between EC338 and EC333 occurred in LOC104426602. According to our SNP analysis, EC338 had 626 more high-impact mutation loci than the male parent P9060 and 396 more than the female parent W1767; W1767 had 259 more mutation loci in the downstream region than EC338, while P9060 had 3107 fewer mutation loci in the downstream region than EC338. Additionally, EC338 had 9631 more mutation loci in the exon region than EC333. Modules were found via WGCNA that were strongly and oppositely correlated with EC338 and EC333, such as module MEsaddlebrown, likely associated with leaf blight resistance. The present study provides a detailed explanation of the genetic basis of eucalypt leaf blight resistance, providing the foundation for exploring genes related to this phenomenon.