Mechanism Of Interaction Research Articles

Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury

Sepsis-induced acute respiratory distress syndrome (ARDS) is a severe complication of sepsis and the leading cause of mortality. Although the role of alveolar macrophages (AMs) in stabilizing pulmonary homeostasis is well established, the effects of circulating extracellular vesicles (EVs) on AMs remain largely unknown. In this study, an investigation was conducted to map the miRNA and protein expression profiles of EVs derived from septic plasma. Notably, EV-based panels (miR-122-5p, miR-125b-5p, miR-223-3p, OLFM4, and LCN2) have been found to be associated with the severity or prognosis of sepsis, with promising AUC values. Moreover, the levels of LCN2, miR-122-5p, and miR-223-3p were identified as independent predictors of septic ARDS. The in vitro coculture results revealed that the effects of LPS-EVs from the plasma of sepsis-induced acute lung injury (ALI), which carry pro-inflammatory EVs, were partly mediated by miR-223-3p, as evidenced by the promotion of inflammation, autophagy and ferroptosis in AMs. Mechanistically, the upregulation of miR-223-3p in LPS-EVs triggers autophagy and ferroptosis in AMs by activating Hippo signaling via the targeting of MEF2C. In vivo, the inhibition of miR-223-3p effectively mitigated LPS-EV-induced inflammation and AM death in the lungs, as well as histological lesions. Overall, miR-223-3p in LPS-EVs contributes to sepsis-induced ALI by priming AMs for autophagy and ferroptosis through the MEF2C/Hippo signaling pathway. These findings suggest a novel mechanism of plasma-AM interaction in sepsis-induced ALI, offering a plausible strategy for assessing septic progression and treating lung injury.

Reviewing the Mechanisms of the Interaction of Characteristic Aroma Compounds of Longjing Tea with S-curve, Molecular Docking, and Molecular Dynamics Simulation: With a Focus on (+)-(1R,2S)-Methyl Epijasmonate and β-Ionone as Examples.

The S-curve method, molecular docking, and molecular dynamics simulation (MDS) were used to explore the interaction between the aroma compounds of Longjing tea and receptors. The ratio of experimental and theoretical threshold (D) values for (+)-(1R,2S)-methyl epijasmonate and β-ionone was 0.46, indicating a synergistic effect between them. Similarly, synergistic interactions occurred between (R)-(+)-γ-nonanolactone and (R)-(+)-γ-decalactone (D = 0.42), 3-methylbutanal and 2-ethyl-3,5-dimethylpyrazine (D = 0.43). Molecular docking indicates significant changes in the affinity and stability between the binary system and individual compounds with receptors (OR52D1 and OR1A1). Through MDS analysis, it was found that the hydrophobic residues in OR52D1-β-ionone changed from Leu203, Val205, Ala206, Ala258, and Met210 to Tyr111, Ala112, Met210, Ala209, and Ala206 after addition of (+)-(1R,2S)-methyl epijasmonate. Hydrophobic interactions play an integral and important role in the formation of a stable ternary architecture between (+)-(1R,2S)-methyl epijasmonate-β-ionone and OR52D1. By calculating the binding energy of MMGBSA, it was found that the addition of (+)-(1R,2S)-methyl epijasmonate reduced the energy of the binary system from -53.58 to -58.48 kcal/mol, indicating better stability of the new system. The molecular mechanisms of interactions between the characteristic compounds of Longjing tea were revealed, thus providing theoretical support for improving the flavor of Longjing tea.

FORMATION AND DEVELOPMENT OF MECHANISMS OF INTERACTION BETWEEN PUBLIC GOVERNMENT BODIES AND MASS MEDIA IN UKRAINE

The article analyses the processes of formation and development of the mechanisms of public management of the interaction between the government and the media in modern Ukraine in peacetime and wartime, and on this basis, a number of promising ways of improving the state information policy in Ukraine are determined. A detailed analysis of the formation of world approaches to the meaningful essence and forms of interaction between institutions of public governance and the mass media was carried out. The processes of formation and development of state information policy in independent Ukraine are characterized, and several models of interaction between state authorities and mass media are highlighted. The peculiarities of the interaction between the authorities and the media in wartime conditions and the conduct of an information war against Ukraine by the aggressor country are determined. The main problems and prospects for improving the mechanisms of interaction between the authorities and the media in the context of ensuring information security and information sovereignty in Ukraine are outlined. It was concluded that effective interaction between the authorities and the media plays a key role in the consolidation of the Ukrainian people, ensuring information security and transparency of the authorities' activities. Only the government's willingness to partner with the media and define common goals can ensure an effective information and communication policy, contribute to social progress and European integration of Ukraine. Therefore, the main task of state policy in the media sphere should be the support of democratic principles, the definition of clear criteria for the interaction between the authorities and the media, as well as the implementation of public control mechanisms for the transparency of decision-making.

Prevalence and Associated Factors of Schistosoma mansoni and Other Intestinal Helminthes Co-Infection Among Malaria Positive Patients in Malaria Endemic Areas of Northeast, Ethiopia: A Cross-Sectional Study.

Schistosoma mansoni and malaria share a similar epidemiological distribution or co-endemicity. Co-infection are a global public health burden where epidemiological evidence is crucial to taking evidence-based intervention. Thus, the aim of this study was to assess the prevalence and associated factors of Schistosoma mansoni and other intestinal Helminthes co-infection among malaria positive patients in malaria endemic areas of Northeast Ethiopia. A cross-sectional study was conducted from September 2018 to June 2019 among randomly recruited 145 microscopically confirmed malaria patients in Kemisse and Chefa Robit, Northeast Ethiopia. A pre-tested semi-structured questionnaire was used for sociodemographic and other risk factor data; blood samples for malaria microscopy and stool samples for S. mansoni and other intestinal Helminthes examinations were collected from each participant. STATA 17 was used for analysis. Chi-square and Fishers' exact test were used as required. The internal consistency and model good ness of fitness test were checked using Cronbach's alpha and Hosmer-Lemshow test, respectively. Bivariable and multivariable logistic regression model was used for analysis. Finally, variables with p < 0.05, their AOR and their 95% Confidence Intervals were considered statistically significant. A total of 145 patients with malaria were included in this study of which 11.3% and 49.0% respectively had poly and mono infections. From all malaria confirmed patients, 29.7% were positive for S. mansoni, which was significantly associated with pervious intestinal helminthic infection, history of swimming and fishing participants who lives near to river and having a history of crossing river by their legs without shoe. Moreover, compared with students; farmers merchants and housewives were found to be highly affected. The co-endemicity of S. mansoni and malaria in the current study was considerably high. Further study is needed to explore the underlying mechanisms of interaction between malaria and S. mansoni with larger sample size.

Evaluation of the effects of amitraz on the enzyme activity and stability of lysozyme: Spectroscopic and MD simulation approach.

The binding interaction of food preservatives and pesticides has emerged as a matter of paramount importance as it not only presents potential health hazards but also carries substantial consequences for food processing and preservation. Herein, the mechanism of interaction between lysozyme and Amitraz was explored through spectroscopic and computational techniques. Spectral investigations indicated the spontaneous nature and stability of the lysozyme-Amitraz complex. The corresponding CD and FT-IR studies proved the structural changes of lysozyme. The presence of amitraz led to a notable decrease in both the enzymatic activity and thermal stability of lysozyme. Molecular docking demonstrated the preferred mode of interaction, and molecular dynamics simulations confirmed the stability of the resultant complex. In conclusion, the alarming findings of the lysozyme-Amitraz interaction underscore its detrimental impact on food safety and human health. Accordingly, urgent measures are imperative to address and mitigate the potential hazards posed by such interactions in food production.

Towards microbial consortia in fermented foods for metabolic engineering and synthetic biology.

The fermented foods microbiota, whose community structures evolve through a succession of different microbial groups, play a central role in fermented food production. The texture and flavor, functions, shelf-life and safety, are largely determined by the interactions among bacteria and yeast within these communities. Although much indispensable work has described the microbial composition and succession in various fermentation foods, yet the specific microbial interactions involved are not well understood. Here, we review the current mechanisms of microbial interactions (amensalism, competition, commensalism, and mutualism) existed in the fermented foods. We also examine the function of these interactions. In addition, we provide our perspectives on the future development of functional and novel fermented foods by combining the new starter cultures with the native microbial consortia and applications of these stable and robust microbial consortia for metabolic engineering and synthetic biology.

Research on the prediction of mechanical properties of magnesium-silicon-based cement and the mechanism of element interaction based on machine learning

Research on the prediction of mechanical properties of magnesium-silicon-based cement and the mechanism of element interaction based on machine learning

Deletion of BMP4 impairs trophoblast function and decidual macrophage polarization via autophagy leading to recurrent spontaneous abortion.

Bone morphogenetic protein 4 (BMP4) is widely involved in the regulation of cell proliferation and differentiation, but its role in Recurrent Spontaneous Abortion (RSA) remains unclear. RSA is a disease that affects roughly 1-2% of partner pairs, but its pathogenesis is still unclear. In recent years, many studies have focused on the role of decidual macrophages in RSA. In this study, we found decreased expression levels of BMP4 in villous tissues of RSA patients and found that low expression of RUNX2 leads to down-regulation of BMP4, which impairs trophoblast function. More importantly, we found in both co-culture system and human recombinant BMP4 protein models that BMP4 overexpression polarizes THP-1-derived macrophages toward M2, and down-regulation of BMP4 leads to macrophage polarization toward M1. Mechanically, we found that BMP4 promotes macrophage polarization via regulating autophagy level. The recovery experiment was further confirmed that 3-MA (autophagy inhibitor) inhibit THP-1-derived macrophage polarization toward M2 induced by BMP4 overexpression and exogenous addition of rBMP4, and rapamycin (autophagy agonists) inhibit macrophages polarization toward M1 from down-regulation of BMP4. Our study further reveals the mechanism of maternal-fetal interface cell interactions in RSA, which can help in the diagnosis and treatment of RSA.

Investigating the binding of fluorescent probes to a trypanosomal-tRNA synthetase: A fluorescence spectroscopic and molecular dynamics study.

Given the high prevalence of Chagas disease in the Americas, we targeted the unique arginyl-tRNA synthetase of its causative agent Trypanosoma cruzi. Among their many possible uses, naphthalene-derived fluorescent ligands, such as ANS and bis-ANS, may be employed in pharmacokinetic research. Although ANS and bis-ANS have become prominent fluorescent probes for protein characterization, the structural and spectroscopic characteristics of protein-ANS/bis-ANS complexes remain largely unknown. Both fluorescent dyes bind to either the folded or partially folded hydrophobic regions of proteins. Additionally, they serve to identify molten globule-like intermediates. These probes have been used to study the folding problems of protein structures and the mechanisms of protein-protein interactions. ANS and bis-ANS exhibited significant enhancement and blue shift in their emission spectra upon binding to TcArgRS, the primary enzyme responsible for attaching l-arginine to its corresponding tRNA. Through fluorescence spectroscopy and computational studies, we concluded that bis-ANS binds more tightly to TcArgRS and that ATP affects bis-ANS fluorescence signal. Thus, these probes are useful resources for studying the intricate intermolecular relationships between proteins in terms of their structure, function, and mechanism. Our study provides a framework for identifying the hydrophobic regions present in TcArgRS. The utilization of hydrophobic patches on proteins for drug targeting is noteworthy because they can assist in identifying regions on the surface of proteins that are likely to interact with ligands. These patches help identify hotspot residues that play a vital role in determining binding affinity. Drugs are mainly small and hydrophobic in nature, and they target protein surfaces which have complementary properties. In this study, we elucidated the potential of TcArgRS as a target for combating trypanosomal diseases and extending life expectancy.

Forced motion modes of a patch of flexible rods in open channel flows

Flexible vegetation is common in riverine and coastal ecosystems. The patch of flexible vegetation is influenced by various factors, resulting in a series of characteristic modes of motion. This study aims to investigate the influence of the Cauchy number (Ca, the ratio of the hydrodynamic drag force to the bending rigidity, i.e., the inverse of the dimensionless bending rigidity) and the solid volume fraction (which reflects the distribution density of a patch of flexible rods) on the motion modes. Additionally, the induction mechanism of fluid and flexible vegetation interaction on motion modes is investigated. A semi-resolved coupling numerical model was employed to simulate the fluid and structure interaction. The results show that the Cauchy number and solid volume fraction influence the motion modes to different extents. Notably, the effect of Ca was significant within the designed range of the two parameters. The wavelength of the monami mode generally shows little variation compared to that observed in the Kelvin–Helmholtz (K-H) vortex, which results from the mixing layer flow instability. The physical mechanism that the motion mode of a patch of flexible rods is induced by the interaction between water flow and flexible rods is further validated and cognized. When the K-H vortex frequency, the natural frequency of the patch in water, and the motion frequency of the patch are close, the patch exhibits motion in the monami mode. Otherwise, the patch will exhibit motion in erect, swaying, and prone mode.

Interaction of biomimetic lipid membranes with detergents with different physicochemical characteristics.

Membrane solubilization by detergents is routinely performed to separate membrane components, and to extract and purify membrane proteins. This process depends both on the characteristics of the detergent and properties of the membrane. Here we investigate the interaction of eight detergents with very distinct physicochemical features with model membranes in different biologically relevant phases. The detergents chosen were the non-ionic Triton X-100, Triton X-165, C10E5, octyl glucopyranoside (OG) and dodecyl maltoside (DDM) and the ionic sodium dodecyl sulfate (SDS), cetyl trimethyl ammonium bromide (CTAB) and Chaps. Three lipid compositions were explored: pure palmitoyl oleoyl phosphatidylcholine (POPC), in the liquid-disordered (Ld) phase, sphingomyelin (SM)/cholesterol 7:3 (chol) in the liquid-ordered (Lo) phase and the biomimetic POPC/SM/chol 2:1:2, which might exhibit Lo/Ld phase separation. Turbidity measurements of small liposomes were performed along the titration with the detergents to obtain the overall solubilization profiles and optical microscopy of giant unilamellar vesicles (GUVs) was used to reveal the mechanism of interaction of the detergents. The presence of cholesterol renders the membranes partly/fully insoluble in all detergents, and the charged detergents are the least effective to solubilize POPC. The non-ionic detergents, with exception of DDM, with the bulkiest headgroup, caused a substantial increase in surface area of POPC, which was quantified directly on single GUVs. The other detergents induced mainly vesicle burst. Detergents that caused some increase in area induced Lo/Ld phase separation in the ternary mixture, with preferential solubilization of the latter. The insoluble area fraction left intact was quantified. Overall, the non-ionic detergents were the most effective in solubilizing lipid membranes.

Virus-host immune interaction in asymptomatic HTLV-1 carriers.

Human T-cell leukemia virus type 1 (HTLV-1) can cause fatal diseases, including adult T-cell leukemia in humans after long-term asymptomatic infection. In asymptomatic HTLV-1 carriers, substantial proviruses are detectable in lymphocytes, and the association of a higher proviral load with a higher risk of disease progression has been observed. However, viral replication is controlled and HTLV-1 production is poor in asymptomatic carriers. Virus-host immune interaction during latent infection has not been fully determined. In the present study, virus-specific antibody and T-cell responses in asymptomatic HTLV-1 carriers were investigated. Neutralizing antibody responses were positively correlated with proviral loads. Tax-specific CD8+ T-cell frequencies were not associated with proviral loads but inversely correlated with the ratios of p19-expressing CD4+ T-cell frequencies to proviral loads, supporting the notion that Tax-specific CD8+ T-cell responses play an important role in the control of HTLV-1 replication. These results provide insights into the mechanism of virus-host immune interaction in latent HTLV-1 infection.

A physicochemical investigation of the complex formation by β-cyclodextrin with Triton X-100 and Triton X-114 and their aggregation behaviour in aqueous solution: an experimental approach.

The complexation behavior and binding affinity of Triton X-100 (TX-100) and Triton X-114 (TX-114) with β-cyclodextrin (β-CD) were extensively studied in an aqueous medium using a comprehensive suite of experimental techniques. These techniques allowed for the evaluation of key physicochemical parameters, including critical micelle concentration (cmc), aggregation number (Nagg), Stern-Volmer constant, and particle size distribution. These metrics were instrumental in understanding the underlying mechanism of the host-guest interaction between β-CD and Triton-X. Dynamic light scattering (DLS) data provided strong evidence for the formation of inclusion complexes, demonstrating significant hydrophobic interactions between the hydrophobic regions of Triton-X and the cavity of β-CD. The disruption of micellar structures, caused by β-CD encapsulating the hydrophobic moieties of the surfactants, was clearly observed. This process also resulted in an increased CMC, further underscoring the impact of β-CD on the aggregation behavior of the surfactants. To quantify the interaction, the Benesi-Hildebrand method was utilized to determine the stoichiometry and binding constants of the β-CD/Triton-X complexes. The results confirmed a well-defined 1 : 1 binding mode, indicating the precise incorporation of the surfactant's hydrophobic tails into the β-CD cavity while leaving the hydrophilic regions exposed to the aqueous environment. This selective binding mechanism alters the thermodynamics of micellization and disrupts the native micellar equilibrium of the surfactant systems. This systematic and comparative investigation is among the few studies that thoroughly examine the interactions between Triton-X surfactants and β-CD. Such research not only enhances our understanding of these complexes, but also reveals their significant potential for various applications. In drug delivery, for example, β-CD/Triton-X complexes can improve the solubility, stability, and bioavailability of hydrophobic drugs. In supramolecular chemistry, these complexes serve as model systems for studying host-guest interactions and self-assembly processes. Furthermore, their ability to modulate surfactant behaviour opens avenues for their use in material science, cosmetics, and industrial formulations, where precise control over micelle formation and aggregation is essential. This study underscores the versatility and utility of β-CD in interacting with non-ionic surfactants, offering insights that can be applied to other amphiphilic systems and paving the way for innovative applications in diverse fields.

Microscopic Heterogeneity Driven by Molecular Aggregation and Water Dynamics in Aqueous Osmolyte Solutions.

Water dynamics are investigated in binary osmolyte-water mixtures, exhibiting a microscopic heterogeneity driven by molecular aggregation, on the basis of molecular dynamics (MD) simulation studies. The protecting osmolyte TMAO molecules in solution are evenly dispersed without the formation of noticeable osmolyte aggregates, while the denaturant TMU molecules aggregate readily, generating microscopic heterogeneity in the spatial distribution of component molecules in TMU-water mixtures. A combined study of MD simulation with graph theoretical analysis and spatial inhomogeneity measurement with h-values in the two osmolyte solutions revealed that the translational and rotational motions of water in the microheterogeneous environment of TMU-water mixtures are less hindered than those in the homogeneous media of TMAO-water mixtures. The analysis of the osmolyte-water H-bond lifetime in the binary solutions shows that destabilizing osmolyte TMU makes relatively weak osmolyte-water interaction, compared to that in protecting osmolyte TMAO, enabling the interplay of TMU-TMU or TMU-protein as well as TMU-water interaction. Taken together, the complementary contributions of the two hypotheses are proposed to elucidate the operating mechanism of the osmolyte on protein stability, encompassing a direct mechanism for the preferential interaction between the osmolyte and protein and an indirect mechanism for the modulation of the water structure and dynamics in the osmolyte solutions.

MSphere of Influence: Seeking the unseen fungi in tumors.

Ningning Liu works in the field of fungal infection and cancer progression, with a particular focus on the mechanism of host-pathogen interaction. In this mSphere of influence article, he reflects on how papers entitled "The fungal mycobiome promotes pancreatic oncogenesis via activation of MBL," by B. Aykut, S. Pushalkar, R. Chen, Q. Li, et al. (Nature 574:264-267, 2019, https://doi.org/10.1038/s41586-019-1608-2), and "A pan-cancer mycobiome analysis reveals fungal involvement in gastrointestinal and lung tumors," by A. B. Dohlman, J. Klug, M. Mesko, I. H. Gao, et al. (Cell 185:3807-3822.E12, 2022, https://doi.org/10.1016/j.cell.2022.09.015), emphasized the non-negligible role of fungi in the host and demonstrated a connection between fungi and cancer. These researches arouse his interest in seeking the novel fungal pathogen lurking inside tumors and understanding the unexplored mechanisms behind the severe fungal infections in cancer patients.

Конверс-анализ обыденной речи: русский, французский и английский языки

The aim of the study is to identify the characteristics of the current state of the corpus of conversational analysis methods by examining, on the basis of video texts in different European languages, how conversation analysis articulates verbal and non-verbal data, discourse formats and social categories of interactants, taking into account the systematicity and variability of multimodal formats of communicative steps. The scientific novelty of the work consists in constructing a universal linguo-semiological model for describing everyday discourse in order to clarify the conceptual apparatus and more clearly semantic structuring of the terminology of linguistics and conversation analysis, as well as the theory of language and communication theory. The key point of the study is the attitude to language as an open, actively developing system of conversational supports and guidelines used to achieve the goals of interaction with other individuals, which cannot be interpreted without involving non-verbal communication resources. The multimodal method of processing information at the junction of linguistics and converse analysis in the mainstream of the interpretative-semiological approach is also important. The data obtained make it possible to identify a number of important mechanisms of linguosemiotic interaction between speech subjects, necessary for constructing an adequate communication model associated with the activation of the dynamics of the polyfunctional parameters of the speech act, on the paths of creating a new metadiscourse axiology both in theory and in practice not only of linguosemiotics of converse analysis, but also of general linguistics.

A Comprehensive Review of Riverbank Filtration Technology for Water Treatment

Riverbank filtration (RBF) technology has been applied and investigated worldwide for water supplies due to its sustainable water quantity guarantee and reliable quality improvement. In this work, the development history, application status, research progress, and technical overview of RBF are reviewed and summarized. RBF usually uses rivers, lakes, and groundwater as raw water, with a few cases using seawater. Nitrogen removal in RBF systems primarily occurs through key geochemical processes such as adsorption, denitrification, organic nitrogen mineralization, and dissimilatory nitrate reduction to ammonium (DNRA). For the attenuation of emerging contaminants in groundwater environments, key processes such as filtration, adsorption, and biotransformation play a crucial role, and microorganisms are essential. Based on a discussion of the advantages and disadvantages, we proposed the research prospects of RBF. To further enhance the water-supply safety and security with RBF, the mechanisms of surface water and groundwater interaction, pollutant removal, and blockage; the impact of capturing surface water on the stability of river ecosystems; and the coupling and synergistic effect of RBF with other water treatment technologies should be deeply investigated.

Investigation of Pyrrole Analogues Inhibition on Mpro by Spectroscopic Analysis, Molecular Docking and Enzyme Activity.

The main protease (Mpro) is a cysteine enzyme and represents a vital target for antiviral drug screening. In this work, Twenty-five pyrrole derivatives were synthesized and screened by enzyme activity experiments. Results indicate that six pyrrole derivatives can bind to Mpro and have inhibitory effect on Mpro. The binding mode involves a static quenching process. Among them, 1d exhibits the highest binding affinity. The interactions between pyrrole derivatives and Mpro are investigated by spectra and molecular docking. The interaction between 1d and Mpro is spontaneous and enthalpy-driven. Hydrogen bonding is identified as the primary binding force for 1d. Furthermore, nitro groups are important for the binding between pyrrole analogues and Mpro. This study elucidates the mechanism of interactions and provides direction and valuable insights for developing novel Mpro inhibitors.

Social interactions in the digital environment: sociolinguistic approach

The article reveals the methodological application of sociolinguistic approach in the process of researching the relationship between lexical markers and social interactions in the digital environment. Social interaction in virtual space is investigated through the identification of mechanisms of social field construction through the symbolic nature of the text. As a method, a social network analysis is proposed, which relies on graph theory and visualisation of nodes and edges (‘nodes’) and links (‘links’) with certain types of relationships. The technology of building a corpus of key words and phrases is described, on the basis of which the structure is formed, clusters and the most significant lexical units reflecting the main discourses, themes and frames are singled out. The application of the network analysis method in the empirical field can reveal the mechanisms of interaction between different social groups.

HSP27/IL-6 axis promotes OSCC chemoresistance, invasion and migration by orchestrating macrophages via a positive feedback loop

Novel strategies to disrupt tumor progression have emerged from studying the interactions between tumor cells and tumor-associated macrophages (TAMs). However, the molecular mechanisms of interactions between tumor cells and TAMs underlying oral squamous cell carcinoma (OSCC) progression have not been fully elucidated. This study explored the molecular mechanism of the HSP27/IL-6 axis in OSCC chemoresistance, invasion, and migration. Here, we demonstrated the higher expression of HSP27 in OSCC cells. Paracrine HSP27 from OSCC cells enhanced chemoresistance, invasion, migration, and EMT in OSCC by inducing M2 polarization and IL-6 secretion in TAMs. HSP27 and IL-6 established a positive feedback loop between OSCC cells and M2 TAMs. TAMs-derived IL-6 orchestrated OSCC stemness and chemoresistance through upregulating β-catenin and CD44, and enhanced OSCC invasion, migration, and EMT via autocrine HSP27/TLR4 signaling. Collectively, HSP27/IL-6 axis facilitates OSCC chemoresistance, invasion, and migration by orchestrating macrophages through a positive feedback loop. We identify the regulatory mechanism underlying the interaction and crosstalk between OSCC cells and TAMs mediated by the HSP27/IL-6 axis. Targeting the HSP27/IL-6 axis could be a promising treatment strategy for OSCC patients, potentially controlling disease progression and improving prognosis and recurrence outcomes.Graphical Graphical Headlights1. HSP27/IL-6 axis promotes OSCC chemoresistance, invasion, and migration by promoting M2 polarization and IL-6 secretion of TAMs.2. HSP27/IL-6 mediates a positive feedback loop between OSCC cells and M2 TAMs.3. Targeting HSP27/IL-6 axis might be a promising treatment strategy for OSCC.