We previously found that nusD-type mutations in Escherichia colitranscription termination factor Rho enhance in vitrotranscription termination at four points within the λ crogene. Here we show that the early termination points are part of one Rho-dependent termination site, tRE, with properties like those of previously characterized Rho-dependent sites λ tR1 and trpt′. The early termination points are all RNA polymerase pause sites, and by deletion analysis and oligonucleotide blocking experiments, a common 5′ Rho entry site for the early termination points ( rutE) is identified. We show that both Rho026 and Rho +can use rutEas an entry point for termination, but that Rho026 is more efficient in releasing the nascent RNA at tRE. The RNA-dependent ATPase activities of wild-type and mutant Rhos are similar, as are their abilities to bind free RNA and to use (rC) 10oligomers for ATPase activation. We therefore suggest that Rho-RNA polymerase interactions that define the site of RNA 3′ end formation are altered in NusD Rho mutants. NusD Rho mutants are less dependent on, but still responsive to, the transcription termination factor NusG. However, addition of NusG to in vitrotermination assays allows Rho +to terminate more efficiently at tRE. These results suggest that NusG aids in the 3′ end formation process. The decreased dependence on NusG for termination by the mutant Rhos in vitroprovides an explanation for poorer λ growth in rho(nusD)cells by interference with λN-mediated antitermination at Rho-dependent sites.