Mechanism Of Pore Formation Research Articles

Analysis of pore formation and carburizing mechanism of magnetite reduction by CO

To investigate the porosity and expansion mechanism of CO-reduced magnetite, the reduced microstructures of magnetite under conventional heating and microwave heating were compared. The microstructure characteristics of magnetite with various reduction degrees were examined in a 60%CO-Ar reduction atmosphere, and the formation and evolution mechanism of the structure were analyzed in conjunction with the reduction effect. The results indicate that the reduction product of CO reduced magnetite possesses a porous structure. Nevertheless, when the gas diffusion is restricted, the reduction process is sluggish and the porous structure is not readily formed. Microwave heating enhances the gas-phase diffusion capacity, thermodynamic carburizing capacity, and solid-phase carbon-oxygen diffusion capacity, resulting in a higher oxygen loss rate than that of conventional heating. Under the experimental conditions, the lattice shrinkage during the Fe3O4 → Fe0.88O transformation and the rapid expansion of Fe0.88O → Fe0.94O are the causes of the crack. Microwaves facilitate the formation of pores and cracks in the product. This study offers a novel concept for the development of foam steel.

Pyroptotic executioner pore-forming protein gasdermin D forms oligomeric assembly and exhibits amyloid-like attributes that could contribute for its pore-forming function.

Gasdermin D (GSDMD) is the chief executioner of inflammatory cell death or pyroptosis. During pyroptosis, proteolytic processing of GSDMD releases its N-terminal domain (NTD), which then forms large oligomeric pores in the plasma membranes. Membrane pore-formation by NTD allows the release of inflammatory cytokines and causes membrane damage to induce cell death. Structural mechanisms of GSDMD-mediated membrane pore-formation have been extensively studied. However, less effort has been made to understand the physicochemical properties of GSDMD and their functional implications. Here, we explore detailed characterization of the physicochemical properties of mouse GSDMD (mGSDMD), and their implications in regulating the pore-forming function. Our study reveals that mGSDMD shows some of the hallmark features of amyloids, and forms oligomeric assemblies in solution that are critically dependent on the disulfide bond-forming ability of the protein. mGSDMD oligomeric assemblies do not resemble typical amyloid fibrils/aggregates, and do not show resistance to proteolytic degradation that is otherwise observed with the conventional amyloids. Our results further elucidate the essential role of an amyloid-prone region (APR) in the oligomerization and amyloid-like features of mGSDMD. Furthermore, alteration of this APR leads to compromised pore-forming ability and cell-killing activity of NTD released from mGSDMD. Taken together, our study for the first time provides crucial new insights regarding implications of the amyloid-like property of mGSDMD in regulating its pore-forming function, which is an essential requirement for this pyroptotic executioner. To the best of our knowledge, such mode of regulation of mGSDMD-function has not been appreciated so far.

Pore formation and evolution mechanisms during hydrocarbon generation in organic-rich marl

Marine organic-rich marl is not only a high-quality hydrocarbon source of conventional oil and gas, but also a new type and field of unconventional oil and gas exploration. An understanding of its pore structure evolution characteristics during a hydrocarbon generation process is theoretically significant and has application prospects for the exploration and development of this special type of natural gas reservoirs. This study conducted thermal simulation of hydrocarbon generation under near-geological conditions during a whole process for cylinder samples of low mature marine organic-rich marl in the Middle Devonian of Luquan, Yunnan Province, China. During this process, hydrocarbon products at different evolution stages were quantified and corresponding geochemical properties were analyzed. Simultaneously, field emission scanning electron microscopy (FE-SEM) and low-pressure gas adsorption (CO2, N2) tests were applied to the corresponding cylinder residue samples to reveal the mechanisms of different types of pore formation and evolution, and clarify the dynamic evolution processes of their pore systems. The results show that with an increase in temperature and pressure, the total oil yield peaks at an equivalent vitrinite reflectance (VRo) of 1.03% and is at the maximum retention stage of liquid hydrocarbons, which are 367.51 mg/g TOC and 211.67 mg/g TOC, respectively. The hydrocarbon gas yield increases continuously with an increase in maturity. The high retained oil rate at the peak of oil generation provides an abundant material basis for gas formation at high maturity and over-maturity stage. The lower limit of VRo for organic matter (OM) pore mass development is about 1.6%, and bitumen pores, organic-clay complex pores together with intergranular pores, grain edge seams and dissolution pores constitute a complicated pore-seam-network system, which is the main reservoir space for unconventional carbonate gas. Pore formation and evolution are controlled synergistically by hydrocarbon generation, diagenesis and organic-inorganic interactions, and the pattern of pore structure evolution can be divided into four stages. A pore volume (PV) and a specific surface area (SSA) are at their highest values within the maturity range of 1.9% to 2.5%, which is conducive to exploring unconventional natural gas.

Electrokinetics-Driven Anodic Oxide Pore Formation: Linear and Weakly Nonlinear Analysis

Abstract Anodization of aluminium in an acidic medium facilitates the formation of well-ordered nanoporous anodic oxide films. The mechanism of pore formation is investigated as a morphological instability using a simplified model. The model accounts for the high field conduction law and field-assisted reactions (oxide formation/dissolution) only at the oxide-solution interface. The role of Butler–Volmer electrokinetics, electrolyte pH, anodic efficiency, and interface curvature on reaction kinetics are taken into account. Linear stability analysis suggests that the oxide film is unstable to well-defined wavelengths in specific ranges of parameters such as anodizing efficiency, applied voltage and electrolyte pH. Subsequently, a weakly nonlinear analysis is carried out to determine the nature of bifurcation beyond the stability threshold. Our findings indicate that the instability exhibits a subcritical nature, well in agreement with experimental observations.

Dissecting the Membrane Association Mechanism of Aerolysin Pores at Femtomolar Concentrations Using Water as a Probe.

Aerolysin is a bacterial toxin that forms transmembrane pores at the host plasma membrane and has a narrow internal diameter and great stability. These assets make it a highly promising nanopore for detecting biopolymers such as nucleic acids and peptides. Although much is known about aerolysin from a microbiological and structural perspective, its membrane association and pore-formation mechanism are not yet fully understood. Here, we used angle-resolved second harmonic scattering (AR-SHS) and single-channel current measurements to investigate how wild-type (wt) aerolysin and its mutants interact with liposomes in aqueous solutions at femtomolar concentrations. Our AR-SHS experiments were sensitive enough to detect changes in the electrostatic properties of membrane-bound aerolysin, which were induced by variations in pH levels. We reported for the first time the membrane binding affinity of aerolysin at different stages of the pore formation mechanism: while wt aerolysin has a binding affinity as high as 20 fM, the quasi-pore and the prepore states show gradually decreasing membrane affinities, incomplete insertion, and a pore opening signature. Moreover, we quantitatively characterized the membrane affinity of mutants relevant for applications to nanopore sensing. Our study provides a label-free method for efficiently screening biological pores suitable for conducting molecular sensing and sequencing measurements as well as for probing pore-forming processes.

Study on the effect of controlled disproportionation pore-forming and relative vacuum mechanism on the reduction rate of continuous magnesium smelting process

Study on the effect of controlled disproportionation pore-forming and relative vacuum mechanism on the reduction rate of continuous magnesium smelting process

Achieving low-porosity AlSi10Mg cladding layers for the additive repair of aluminum alloy parts by directed energy deposition

Directed Energy Deposition (DED) has broad application prospects for repairing damaged aluminum alloy parts such as piston aero-engine casing. However, due to the low laser absorptivity and the complex thermal cycle process, it is challenging to obtain aluminum alloy cladding layers with high-quality morphology and low-porosity by DED method. In this work, a series of individual stages of DED process are studied during single-tracks overlapping and layers stacking. Correspondingly, the within layer scanning strategy, inter-layer stacking strategy, and process parameters are examined carefully. Using the proposed DED processing flow that combines substrate preheating with intermittent process, the deposited layers without balling and collapse are successfully achieved. Furtherly, the bi-directional scanning strategy for single-tracks overlapping within layer is proposed to compensate the thickness nonuniformity caused by heat accumulation. The inter defects identified as “lack of fusion” and “gas pores” are observed in the cladding layers with uniform surface. By analyzing the pore formation mechanism and considering the energy distribution and re-melting, the multi-layer offset stacking strategy is proposed to inhibit the porosity to 0.25 %. Combined with the optimized process parameters, the porosity is reduced up to 0.09 %, ensuring the density of the DED deposition coating. This work provides new pathway and direct guidance for fabricating high-quality AlSi10Mg cladding layers during the DED repairing of valuable aluminum alloy parts.

Antagonistic nanobodies implicate mechanism of GSDMD pore formation and potential therapeutic application

Inflammasome activation results in the cleavage of gasdermin D (GSDMD) by pro-inflammatory caspases. The N-terminal domains (GSDMDNT) oligomerize and assemble pores penetrating the target membrane. As methods to study pore formation in living cells are insufficient, the order of conformational changes, oligomerization, and membrane insertion remained unclear. We have raised nanobodies (VHHs) against human GSDMD and find that cytosolic expression of VHHGSDMD-1 and VHHGSDMD-2 prevents oligomerization of GSDMDNT and pyroptosis. The nanobody-stabilized GSDMDNT monomers partition into the plasma membrane, suggesting that membrane insertion precedes oligomerization. Inhibition of GSDMD pore formation switches cell death from pyroptosis to apoptosis, likely driven by the enhanced caspase-1 activity required to activate caspase-3. Recombinant antagonistic nanobodies added to the extracellular space prevent pyroptosis and exhibit unexpected therapeutic potential. They may thus be suitable to treat the ever-growing list of diseases caused by activation of (non-) canonical inflammasomes.

Synthesis, antimicrobial activity, and mechanistic studies of enterocin DD14, a leaderless two-peptide bacteriocin

Bacteriocins are promising alternatives to antibiotics in the food, veterinary and medical sectors, but their study and use is often hampered by the low yields and high costs associated with their purification from naturally occurring bacteria. Chemical synthesis has emerged as a means to overcome this limitation and design more active variants. In this study, microwave-assisted solid-phase peptide synthesis was used to produce the leaderless two-peptide bacteriocin enterocin DD14 (EntDD14), composed of EntDD14A (44 amino acids) and EntDD14B (43 amino acids). The resulting synthetic peptides, syn-EntDD14A and syn-EntDD14B, were tested against Gram-positive bacteria including Listeria, Staphylococcus and Enterococcus strains. Both peptides were found to be necessary for optimal, but not synergistic, antibacterial activity and to act through a pore-forming mechanism. Both peptides exhibited moderate cytotoxicity against eukaryotic cells.

A modified pore evolution particle model applied to CaCO3 decomposition and CaO sintering during calcium looping CO2 cycles

The calcium looping of calcium-based carbonation/calcination cyclic reaction is an efficient decarbonization technology that can be applied to post-combustion carbon capture, solar thermal storage, and enhanced methane reforming hydrogen. The pore structure generated during the calcination stage greatly affects adsorption performance. Hence, the change of pore structure is crucial during the calcination. Existing models related to pore structure still need to be further improved regarding pore formation mechanisms. In this paper, a modified pore evolution model is proposed. The modified pore evolution model contains the kinetic equation, the CO2 diffusion equation, and the vacancy aggregates equation. The modified model considers the possibility of spontaneous pore formation in the mechanism, which can better fit the experimental results and provide higher simulation accuracy. The model reveals the diffusion of CO2 within the particles during decomposition, and spatiotemporal evolutionary properties of pore parameters. The pore change trend is the most obvious when the initial pores are mainly 3 nm mesopores. The pore evolution is relatively stable when the initial pores are mainly 25 nm mesopores. Besides, the particles experience significant pore migration during the sintering stage when the temperature reaches 1273 K. At 1073 K, the maximum pore size occurring during pore evolution did not exceed 40 nm. The model estimates a maximum pore size of more than 60 nm at 1273 K. The modified model can aid in a deeper comprehension of how the pore structure changes throughout the calcination process.

Free Energy Analysis of Peptide-Induced Pore Formation in Lipid Membranes by Bridging Atomistic and Coarse-Grained Simulations.

Antimicrobial peptides (AMPs) are attractive materials for combating the antimicrobial resistance crisis because they can kill target microbes by directly disrupting cell membranes. Although thousands of AMPs have been discovered, their molecular mechanisms of action are still poorly understood. One broad mechanism for membrane disruption is the formation of membrane-spanning hydrophilic pores which can be stabilized by AMPs. In this study, we use molecular dynamics simulations to investigate the thermodynamics of pore formation in model single-component lipid membranes in the presence of one of three AMPs: aurein 1.2, melittin and magainin 2. To overcome the general challenge of modeling long time scale membrane-related behaviors, including AMP binding, clustering, and pore formation, we develop a generalizable methodology for sampling AMP-induced pore formation. This approach involves the long equilibration of peptides around a pore created with a nucleation collective variable by performing coarse-grained simulations, then backmapping equilibrated AMP-membrane configurations to all-atom resolution. We then perform all-atom simulations to resolve free energy profiles for pore formation while accurately modeling the interplay of lipid-peptide-solvent interactions that dictate pore formation free energies. Using this approach, we quantify free energy barriers for pore formation without direct biases on peptides or whole lipids, allowing us to investigate mechanisms of pore formation for these 3 AMPs that are a consequence of unbiased peptide diffusion and clustering. Further analysis of simulation trajectories then relates variations in pore lining by AMPs, AMP-induced lipid disruptions, and salt bridges between AMPs to the observed pore formation free energies and corresponding mechanisms. This methodology and mechanistic analysis have the potential to generalize beyond the AMPs in this study to improve our understanding of pore formation by AMPs and related antimicrobial materials.

How relevant are sterols in the mode of action of prymnesins?

Prymnesins, produced by the haptophyte Prymnesium parvum, are considered responsible for fish kills when this species blooms. Although their toxic mechanism is not fully understood, membrane disruptive properties have been ascribed to A-type prymnesins. Currently it is suggested that pore-formation is the underlying cause of cell disruption. Here the hypothesis that A-, B-, and C-type prymnesins interact with sterols in order to create pores was tested. Prymnesin mixtures containing various analogs of the same type were applied in hemolysis and cytotoxicity assays using Atlantic salmon Salmo salar erythrocytes or rainbow trout RTgill-W1 cells. The hemolytic potency of the prymnesin types reflected their cytotoxic potential, with approximate concentrations reaching 50 % hemolysis (HC50) of 4 nM (A-type), 54 nM (C-type), and 600 nM (B-type). Variabilities in prymnesin profiles were shown to influence potency. Prymnesin-A (3 Cl) + 2 pentose + hexose was likely responsible for the strong toxicity of A-type samples. Co-incubation with cholesterol and epi-cholesterol pre-hemolysis reduced the potential by about 50 % irrespective of sterol concentration, suggesting interactions with sterols. However, this effect was not observed in RTgill-W1 toxicity. Treatment of RTgill-W1 cells with 10 µM lovastatin or 10 µM methyl-β-cyclodextrin-cholesterol modified cholesterol levels by 20-30 %. Regardless, prymnesin cytotoxicity remained unaltered in the modified cells. SPR data showed that B-type prymnesins likely bound with a single exponential decay while A-types seemed to have a more complex binding. Overall, interaction with cholesterol appeared to play only a partial role in the cytotoxic mechanism of pore-formation. It is suggested that prymnesins initially interact with cholesterol and stabilize pores through a subsequent, still unknown mechanism possibly including other membrane lipids or proteins.

Uncovering the membrane and pore formation mechanisms of the lysozyme membrane made via the amyloid-like assembly

Biomimetic protein membranes have attracted increasing interests because of their distinctive separation properties in the membrane field. Low-cost and stable protein lysozyme has been intensively invetigated to fabricate high-performance sepration membranes via a facile and scalable method of amyloid-like assembly. However, the pore formation mechnism of the lysozyme membrane remains unclear. Herein, the lysozyme membranes were fabricated and the membrane formation mechnism and separation properties were investigated experimentally. Molecular dynamics simulation (MDS) was also employed to reveal the pore formation mechanism of the lysozyme membrane. The fabricated lysozyme membrane achieved a stable high water permeance of 10.2 L m−2 h−1 bar−1, as well as a relatively high separation factor (9.5) towards antibiotic and sodium chloride. MDS results found that the membrane pore size is governed by the amino acid compositions and is negatively dependent on the intermolecular forces among amino acids around the pores. Selective pores with 0.54 nm in diameter are primarily formed by these amino acids including alanine (35 %), aspartic acid (25 %), arginine (15 %), phenylalanine (11 %), and lysine (7 %), in which half of them have hydrophobic side chains with no charge, and another half have hydrophilic side chains with balanced charges. This work may stimulate the development of highly permeable and selective protein membranes by carefully engineering their amino acid compositions with optimal charges and hydrophobicity for generation of numerous selective pores.

Preparation of porous (Mo2/3Y1/3)2AlC and its hydrogen evolution reaction performance

Porous (Mo2/3Y1/3)2AlC MAX phase ceramic was prepared by reaction sintering method using Mo, Y, Al, and graphite powder as raw materials. The influence of changes in Al content on the purity of the obtained samples was investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), Archimedes method, and bubble point method. The conditions for obtaining a pure phase were identified, and the transformation path of the phase during sintering and the mechanism of pore formation were provided. The linear sweep voltammetry (LSV) cure and cyclic voltammetry (CV) cure of MAX were tested using an electrochemical workstation. It can be calculated that MAX exhibits good hydrogen evolution reaction (HER) performance under alkaline conditions due to its high electrochemical active surface area (ECSA) of 373.2 and small Tafel slope of 41.7 ​mV·dec−1.

Photodiode-based porosity prediction in laser powder bed fusion considering inter-hatch and inter-layer effects

Laser powder bed fusion, while promising, faces hurdles in certifying fabricated parts due to cost and complexity, with in-process monitoring emerging as a potential solution. Existing models focus on predicting defects at a given location using the monitoring signals from solely that same location. Hence, these models treat each track or layer independently of the previous and subsequent ones, neglecting potential interdependencies. This study proposed an in-situ, photodiode-based monitoring approach considering inter-hatch and inter-layer effects on porosity formation - factors often overlooked in existing research. Two Ti-6Al-4 V cuboids (10×10×5 mm3) were built with optimized process parameters, with the melt pool continuously monitored at 20 kHz via a co-axially mounted photodiode. The monitoring system captured the integral radiation in the near-infrared spectrum within a field of view centered on the melt pool. The porosity is assessed by X-ray computed tomography (X-CT), serving as ground truth to build supervised machine learning (ML) models. This study considered physical phenomena occurring during the printing process, including remelting of lack of fusion pores by the subsequent layer, keyholes penetrating the current layer hence introducing pores in the layer below, and overlap between adjacent scan tracks. These considerations are critical for a holistic understanding of pore formation mechanisms. Photodiode signals and computed tomography volumes were cropped using windows of four sizes to test the model's pore localization capability. A machine learning model, specifically a Convolutional Neural Network (CNN) - Long Short-Term Memory (LSTM) network, was trained to predict porosities using these window sequences. The CNN extracted spatial features from photodiode signals, addressing inter-hatch effects, while the LSTM captured temporal dependencies across layers, addressing inter-layer effects. The results, with the Area Under the Receiver Operating Characteristic curve (AUC) of 0.91 for pores exceeding 8000 μm3 in volume and 100 μm2 in cross-sectional area, demonstrate the feasibility of the proposed model in detecting pores-level defects. This high defect prediction and positioning accuracy are essential for process control, providing real-time status of the region of interest and informing the controller of pore positions, thus facilitating intra-layer or inter-layer correction.

Effect of laser process parameters on the pores, surface roughness, and hardness of laser selective melting of dental cobalt-chrome alloys.

To address the quality problems caused by high porosity in the preparation of dental cobalt-chrome alloy prosthetics based on selective laser melting (SLM) technology, we investigated the influence mechanism of different forming process parameters on the microstructure and properties of the materials. Moreover, the range of forming process parameters that can effectively reduce defects was precisely defined. The effects of laser power, scanning speed, and scanning distance on the pore properties, surface roughness, and hardness of dental cobalt-chrome alloy were investigated by adjusting the printing parameters in the process of SLM. Through metallographic analysis, image analysis, and molten pool simulation, the pore formation mechanism was revealed, and the relationship between the porosity and energy density of SLM dental cobalt-chrome alloy was elucidated. When the linear energy density was higher than 0.18 J/mm, the porosity defect easily appeared at the bottom of the molten pool. When the laser energy density was lower than 0.13 J/mm, defects occurred in the gap of the molten pool due to insufficient melting of powder. In particular, when the linear energy density exceeded the threshold of 0.30 J/mm or was below 0.12 J/mm, the porosity increased significantly to more than 1%. In addition, we observed a negative correlation between free surface roughness and energy density and an inverse relationship between macroscopic hardness and porosity. On the basis of the conditions of raw materials and molding equipment used in this study, the key process parameters of SLM of molding parts with porosity lower than 1% were successfully determined. Specifically, these key parameters included the line energy density, which ranged from 0.13 J/mm to 0.30 J/mm, and the scan spacing should be strictly controlled below 90 μm.

Preparation of lightweight foam glass-ceramics from copper slag tailings: Secondary aluminum slag as pore-forming agent

To address metallurgical waste residue pollution, this study utilized copper slag tailings (CST) and waste glass (WG) as primary raw materials, with secondary aluminum dross (SAD) as a pore-forming agent, to produce lightweight spontaneous foam glass-ceramics. The effects of raw material ratios, sintering temperature (1120–1160 °C), and sintering time (0–40 min) on foam glass-ceramics properties were investigated, elucidating the correlations between various properties. Under optimal preparation conditions (CST: WG: SAD = 7:11:2, sintered at 1150 °C for 20 min), the foam glass-ceramics exhibited a bulk density of 0.41 g/m³, compressive strength of 2.32 MPa, open porosity of 61.02 %, 2-h water absorption of 83.76 %, and mean pore size of 3.12 mm. The sintering process transformed raw materials into substances like diopside subsilic ferrian, which enhanced the strength of lightweight foam glass-ceramics. Harmful substances in the raw materials can be immobilized within the internal structure of foam glass-ceramics, ensuring excellent environmental safety. Additionally, comprehensive characterization was conducted on the foam glass-ceramics, elucidating their composition, structure, and pore-forming mechanisms.

The minimal membrane requirements for BAX-induced pore opening upon exposure to oxidative stress

Perforation of the outer mitochondrial membrane triggered by BAX and facilitated by its main activator cBID is a fundamental process in cell apoptosis. Here, we employ a newly designed correlative approach based on a combination of a fluorescence cross correlation binding with a calcein permeabilization assay to understand the involvement of BAX in pore formation under oxidative stress conditions. To mimic the oxidative stress, we enriched liposomal membranes by phosphatidylcholines with truncated sn-2 acyl chains terminated by a carboxyl or aldehyde moiety. Our observations revealed that oxidative stress enhances proapoptotic conditions involving accelerated pore-opening kinetics. This enhancement is achieved through increased recruitment of BAX to the membrane and facilitation of BAX membrane insertion. Despite these effects, the fundamental mechanism of pore formation remained unchanged, suggesting an all-or-none mechanism. In line with this mechanism, we demonstrated that the minimal number of BAX molecules at the membrane necessary for pore formation remains constant regardless of BAX activation by cBID or the presence of oxidized lipids. Overall, our findings give a comprehensive picture of the molecular mechanisms underlying apoptotic pore formation and highlight the selective amplifying role of oxidized lipids in triggering formation of membrane pores.

Apicomplexan Pore-Forming Toxins.

Pore-forming toxins (PFTs) are released by one cell to directly inflict damage on another cell. Hosts use PFTs, including members of the membrane attack complex/perforin protein family, to fight bacterial infections and cancer, while bacteria and parasites deploy PFTs to promote infection. Apicomplexan parasites secrete perforin-like proteins as PFTs to egress from infected cells and traverse tissue barriers. Other protozoa, along with helminth parasites, utilize saposin-like PFTs prospectively for nutrient acquisition during infection. This review discusses seminal and more recent advances in understanding how parasite PFTs promote infection and describes how they are regulated and fulfill their roles without causing parasite self-harm. Although exciting progress has been made in defining mechanisms of pore formation by PFTs, many open questions remain to be addressed to gain additional key insights into these remarkable determinants of parasitic infections.

Fabrication and manipulation of hierarchically porous PEEK membranes based on crystallization‐induced phase segregation and sea‐island structures

AbstractPoly ether ether ketone (PEEK) is a premier engineering plastic, which has gained considerable attention in recent years. Our prior research investigated crystallization template techniques and pore formation mechanisms within the PEEK/poly (ether imide) system, paving the way for the development of hierarchically porous membranes (HPMs). Compared to single‐porous membranes, HPMs demonstrate enhanced flux and high mass transfer efficiency simultaneously, effectively mitigating the trade‐off effects. In this study, we focused on fabricating PEEK HPMs through precise manipulation of a polymer ternary blend system. The meticulous tuning enables intricate control of the hierarchical pore morphology of PEEK at two distinct scales. The finite element simulations not only underscored the crucial role of hierarchical pores in bolstering separation efficiency, but also exhibited strong concordance with the experimental findings. This study provides guidance for future optimization of HPMs with superior separation performance.