Mechanism Of Boundary Formation Research Articles

Nonsymbolic numerosity in sets with illusory-contours exploits a context-sensitive, but contrast-insensitive, visual boundary formation process

The visual mechanisms underlying approximate numerical representation are still intensely debated because numerosity information is often confounded with continuous sensory cues (e.g., texture density, area, convex hull). However, numerosity is underestimated when a few items are connected by illusory contours (ICs) lines without changing other physical cues, suggesting in turn that numerosity processing may rely on discrete visual input. Yet, in these previous works, ICs were generated by black-on-gray inducers producing an illusory brightness enhancement, which could represent a further continuous sensory confound. To rule out this possibility, we tested participants in a numerical discrimination task in which we manipulated the alignment of 0, 2, or 4 pairs of open/closed inducers and their contrast polarity. In Experiment 1, aligned open inducers had only one polarity (all black or all white) generating ICs lines brighter or darker than the gray background. In Experiment 2, open inducers had always opposite contrast polarity (one black and one white inducer) generating ICs without strong brightness enhancement. In Experiment 3, reverse-contrast inducers were aligned but closed with a line preventing ICs completion. Results showed that underestimation triggered by ICs lines was independent of inducer contrast polarity in both Experiment 1 and Experiment 2, whereas no underestimation was found in Experiment 3. Taken together, these results suggest that mere brightness enhancement is not the primary cause of the numerosity underestimation induced by ICs lines. Rather, a boundary formation mechanism insensitive to contrast polarity may drive the effect, providing further support to the idea that numerosity processing exploits discrete inputs.

Social categories in the making: construction or recruitment?

Real kinds, both natural and social categories, are characterized by rich inductive potential. They have relatively stable sets of conceptually independent projectable properties. Somewhat surprisingly, even some purely social categories (e.g., ethnicity, gender, political orientation) show such multiple projectability. The article explores the origin of the inductive richness of social categories and concepts. I argue that existing philosophical accounts provide only a partial explanation, and mechanisms of boundary formation and stabilization must be brought into view for a more comprehensive account of inductively rich social categories.

Revealing the Grain Boundary Formation Mechanism and Kinetics during Polycrystalline MoS2 Growth.

Controllable synthesis of MoS2 with desired grain morphology via chemical vapor deposition (CVD) or physical vapor deposition (PVD) remains a challenge. Hence, it is important to understand polycrystalline growth of MoS2 and further provide guidelines for its CVD/PVD growth. Here, we formulate a kinetic Monte Carlo (kMC) model aiming at predicting the grain boundary (GB) formation in the CVD/PVD growth of polycrystalline MoS2. In the kMC model, the grain growth is via kink nucleation and propagation, whose energetic parameters and initial nucleus details are either from first-principles calculations or from experiments. Using the kMC model, we perform extensive simulations to predict the GB formation by using two, three, four, and five initial nuclei and compare the simulation results with previous experimental results. The obtained GB morphologies are in an excellent agreement with those experimental results. These agreements suggest that the proposed kMC model can correctly capture the mechanism and kinetics of GB formation. In particular, we reveal that the formation of smooth/rough GB is dictated by the two growth vectors for the kink propagation at the two associated grain edges, which is validated by our high-resolution scanning transmission electron microscopy images for PVD growth of MoS2 grains. Besides, we have made predictions beyond reproducing experimental observations, including the growth with artificially designed nuclei, the morphology transformation by tuning the Mo and S sources, and the formation of high-quality single-crystalline monolayer MoS2 by using single-crystalline substrates with vicinal steps. Our kMC model may serve as a powerful predictive tool for the CVD/PVD growth of monolayer MoS2 with desired GB configurations.

Omics Studies Revealed the Factors Involved in the Formation of Colony Boundary in Myxococcus xanthus.

Two unrecognizable strains of the same bacterial species form a distinct colony boundary. During growth as colonies, Myxococcus xanthus uses multiple factors to establish cooperation between recognized strains and prevent interactions with unrecognized strains of the same species. Here, ΔMXAN_0049 is a mutant strain deficient in immunity for the paired nuclease gene, MXAN_0050, that has a function in the colony-merger incompatibility of Myxococcus xanthus DK1622. With the aim to investigate the factors involved in boundary formation, a proteome and metabolome study was employed. Visualization of the boundary between DK1622 and ΔMXAN_0049 was done scanning electron microscope (SEM), which displayed the presence of many damaged cells in the boundary. Proteome analysis of the DK1622- boundary disclosed many possible proteins, such as cold shock proteins, cell shape-determining protein MreC, along with a few pathways, such as RNA degradation, phenylalanine, tyrosine and tryptophan biosynthesis, and Type VI secretion system (T6SS), which may play major roles in the boundary formation. Metabolomics studies revealed various secondary metabolites that were significantly produced during boundary formation. Overall, the results concluded that multiple factors participated in the boundary formation in M. xanthus, leading to cellular damage that is helpful in solving the mystery of the boundary formation mechanism.

Microstructural and Mechanical Characterization of the Transition Zone of 9%Ni Steel Cladded with Ni-Based Superalloy 625 by GTAW-HW

9%Ni steel was recently used for the first time in the field of injection unit (IU) for the injection of CO2 into oil wells. Because such steels are operated in H2S medium and are susceptible to sulfide stress cracking, their pipes are cladded with Ni-based superalloy 625 by using gas tungsten arc welding with a hot wire to prevent this phenomenon from occurring. The transition zone of substrate/clad can have high hardness and low toughness, and promote failure of the component during service; therefore, it is very important to know its characteristics. In this work, this transition zone was analyzed through optical and scanning electron microscopy with energy dispersive X-ray spectrometry and electron backscatter diffraction, as well as Vickers microhardness, shear and bend tests. Metallographic analysis identified type I and II boundaries with distinct chemical gradients, MC-type carbides, Laves/γ eutectics, peninsulas macrosegregation, crystallographic texture close to <100> in the clads, residual strain, and drop of microhardness across the transition zone. The clads were approved in the shear and bend tests. This work proposes a new type II boundary formation mechanism in dissimilar welds of steels that do not exhibit the allotropic transformation δ → γ during the welding thermal cycle and do not experience a change in the solidification mode.

Notch-dependent epithelial fold determines boundary formation between developmental fields in the Drosophila antenna.

Compartment boundary formation plays an important role in development by separating adjacent developmental fields. Drosophila imaginal discs have proven valuable for studying the mechanisms of boundary formation. We studied the boundary separating the proximal A1 segment and the distal segments, defined respectively by Lim1 and Dll expression in the eye-antenna disc. Sharp segregation of the Lim1 and Dll expression domains precedes activation of Notch at the Dll/Lim1 interface. By repressing bantam miRNA and elevating the actin regulator Enable, Notch signaling then induces actomyosin-dependent apical constriction and epithelial fold. Disruption of Notch signaling or the actomyosin network reduces apical constriction and epithelial fold, so that Dll and Lim1 cells become intermingled. Our results demonstrate a new mechanism of boundary formation by actomyosin-dependent tissue folding, which provides a physical barrier to prevent mixing of cells from adjacent developmental fields.

Making ethnic boundaries in the society of religious renaissance: Islam and everyday ethnicity in post-Soviet Tatarstan

ABSTRACTThis article investigates the conflict between closely intertwined specific ethnic and universal religious practices that affect the formation and maintenance of ethnic group boundaries in the society of simultaneous Islamic and ethnic renaissances: contemporary post-Soviet Tatarstan. I argue that the negotiation of this conflict produces both reinforcement and erosion of the titular ethnic group boundaries. I pay special attention on ethnicity performance and ethnicity consumption practices. Thus, I conclude that practices of performing and consuming ethnicity serve as effective mechanism of boundary formation not just between various ethnic groups in the multi-ethnic republic but also inside the group itself.

Grain boundary formation by remnant dislocations from the de-twinning of thin nano-twins

We report a grain boundary formation mechanism in face-centred cubic metals with low stacking fault energies. Severe plastic deformation produces primary nano-twins with a twin boundary spacing of several nanometres, followed by secondary twinning through the activation of Shockley partial dislocations. The partial dislocations interact with primary twin boundaries, resulting in de-twinning of the primary twins and producing very high densities of sessile dislocations. The accumulation of these dislocations produces new grain boundaries with neighbouring grains having similar orientations.

Combined lineage mapping and gene expression profiling of embryonic brain patterning using ultrashort pulse microscopy and image registration.

During embryogenesis, presumptive brain compartments are patterned by dynamic networks of gene expression. The spatiotemporal dynamics of these networks, however, have not been characterized with sufficient resolution for us to understand the regulatory logic resulting in morphogenetic cellular behaviors that give the brain its shape. We have developed a new, integrated approach using ultrashort pulse microscopy [a high-resolution, two-photon fluorescence (2PF)-optical coherence microscopy (OCM) platform using 10-fs pulses] and image registration to study brain patterning and morphogenesis in zebrafish embryos. As a demonstration, we used time-lapse 2PF to capture midbrain-hindbrain boundary morphogenesis and a wnt1 lineage map from embryos during brain segmentation. We then performed in situ hybridization to deposit NBT/BCIP, where wnt1 remained actively expressed, and reimaged the embryos with combined 2PF-OCM. When we merged these datasets using morphological landmark registration, we found that the mechanism of boundary formation differs along the dorsoventral axis. Dorsally, boundary sharpening is dominated by changes in gene expression, while ventrally, sharpening may be accomplished by lineage sorting. We conclude that the integrated visualization of lineage reporter and gene expression domains simultaneously with brain morphology will be useful for understanding how changes in gene expression give rise to proper brain compartmentalization and structure.

Two nested developmental waves demarcate a compartment boundary in the mouse lung.

The lung is a branched tubular network with two distinct compartments — the proximal conducting airways and the peripheral gas exchange region — separated by a discrete boundary termed the bronchoalveolar duct junction (BADJ). Here we image the developing mouse lung in three dimensions and show that two nested developmental waves demarcate the BADJ under the control of a global hormonal signal. A first wave of branching morphogenesis progresses throughout embryonic development, generating branches for both compartments. A second wave of conducting airway differentiation follows the first wave but terminates earlier, specifying the proximal compartment and setting the BADJ. The second wave is terminated by a glucocorticoid signaling: premature activation or loss of glucocorticoid signaling causes a proximal or distal shift, respectively, in BADJ location. The results demonstrate a novel mechanism of boundary formation in complex, three-dimensional organs and provide new insights into glucocorticoid therapies for lung defects in premature birth.

Shoot Branching and Leaf Dissection in Tomato Are Regulated by Homologous Gene Modules

Aerial plant architecture is predominantly determined by shoot branching and leaf morphology, which are governed by apparently unrelated developmental processes, axillary meristem formation, and leaf dissection. Here, we show that in tomato (Solanum lycopersicum), these processes share essential functions in boundary establishment. Potato leaf (C), a key regulator of leaf dissection, was identified to be the closest paralog of the shoot branching regulator Blind (Bl). Comparative genomics revealed that these two R2R3 MYB genes are orthologs of the Arabidopsis thaliana branching regulator REGULATOR OF AXILLARY MERISTEMS1 (RAX1). Expression studies and complementation analyses indicate that these genes have undergone sub- or neofunctionalization due to promoter differentiation. C acts in a pathway independent of other identified leaf dissection regulators. Furthermore, the known leaf complexity regulator Goblet (Gob) is crucial for axillary meristem initiation and acts in parallel to C and Bl. Finally, RNA in situ hybridization revealed that the branching regulator Lateral suppressor (Ls) is also expressed in leaves. All four boundary genes, C, Bl, Gob, and Ls, may act by suppressing growth, as indicated by gain-of-function plants. Thus, leaf architecture and shoot architecture rely on a conserved mechanism of boundary formation preceding the initiation of leaflets and axillary meristems.

Rpd3-dependent boundary formation at telomeres by removal of Sir2 substrate

Boundaries between euchromatic and heterochromatic regions until now have been associated with chromatin-opening activities. Here, we identified an unexpected role for histone deacetylation in this process. Significantly, the histone deacetylase (HDAC) Rpd3 was necessary for boundary formation in Saccharomyces cerevisiae. rpd3Delta led to silent information regulator (SIR) spreading and repression of subtelomeric genes. In the absence of a known boundary factor, the histone acetyltransferase complex SAS-I, rpd3Delta caused inappropriate SIR spreading that was lethal to yeast cells. Notably, Rpd3 was capable of creating a boundary when targeted to heterochromatin. Our data suggest a mechanism for boundary formation whereby histone deacetylation by Rpd3 removes the substrate for the HDAC Sir2, so that Sir2 no longer can produce O-acetyl-ADP ribose (OAADPR) by consumption of NAD(+) in the deacetylation reaction. In essence, OAADPR therefore is unavailable for binding to Sir3, preventing SIR propagation.

A genetic screen for modifiers of UFO meristem activity identifies three novel FUSED FLORAL ORGANS genes required for early flower development in Arabidopsis.

In a screen to identify novel genes required for early Arabidopsis flower development, we isolated four independent mutations that enhance the Ufo phenotype toward the production of filamentous structures in place of flowers. The mutants fall into three complementation groups, which we have termed FUSED FLORAL ORGANS (FFO) loci. ffo mutants have specific defects in floral organ separation and/or positioning; thus, the FFO genes identify components of a boundary formation mechanism(s) acting between developing floral organ primordia. FFO1 and FFO3 have specific functions in cauline leaf/stem separation and in first- and third-whorl floral organ separation, with FFO3 likely acting to establish and FFO1 to maintain floral organ boundaries. FFO2 acts at early floral stages to regulate floral organ number and positioning and to control organ separation within and between whorls. Plants doubly mutant for two ffo alleles display additive phenotypes, indicating that the FFO genes may act in separate pathways. Plants doubly mutant for an ffo gene and for ufo, lfy, or clv3 reveal that the FFO genes play roles related to those of UFO and LFY in floral meristem initiation and that FFO2 and FFO3 may act to control cell proliferation late in inflorescence development.

The effect of inducer polarity and contrast on the perception of illusory figures.

A study designed to determine how inducer-surround contrast and inducer polarity affect the contour clarity and the lightness of illusory figures is reported. Using magnitude estimation procedures, ten naive subjects rated both the contour clarity and the lightness of Kanizsa squares. The magnitude of the inducer-surround contrast and the inducer polarity (all-black, all-white, or black-and-white) were varied randomly on each trial. The data indicate that contour clarity increases with contrast at the same rate across polarity conditions but that contour clarity at any given contrast level depends significantly on polarity. Contour clarity judgments were significantly lower when the inducers were all-white than when the inducers were all-black or black-and-white, and significantly greater in the 'mixed' polarity case (black-and-white inducers) than in the 'same' polarity case (the average of the all-black and all-white inducer conditions). Inducer contrast and polarity significantly affected the lightness of the illusory figure in a manner consistent with simultaneous spatial contrast. Also, for a given increment in contrast, contour clarity altered significantly more than surface lightness, regardless of inducer polarity. The findings suggest that the mechanism which mediates boundary formation is sensitive to the direction of contrast, and that the boundary formation mechanism is more sensitive than the surface lightness mechanism to changes in contrast magnitude. The results are considered within the context of neural network models of form perception.

Categorical perception of mouse-pup ultrasounds in the temporal domain

Mouse-pup ultrasounds emitted from outside the nest area are releasers of maternal pup searching and retrieving behaviour. House mouse, Mus domesticus, mothers were tested in a situation with two alternative choices for their unconditioned preference of 50 kHz tone bursts of various durations (models of mouse-pup ultrasounds). Categorical perception in the temporal domain was established by labelling and discrimination tests. Two categories occurred: one of non-preferred short-duration tones (25 ms and shorter) and the other of preferred long-duration tones (30 ms and longer) with a sharp boundary between 25 and 30 ms. Stimuli from the two categories were discriminated, however, only if they differed in duration by at least 20–25ms, which may be the threshold of duration discrimination. The discussion concentrates on the biological significance of categorical perception of communication sounds and possible mechanisms of boundary formation.