Mechanism Of Inhibition Research Articles

Structural insights into the inhibition mechanism of fungal GWT1 by manogepix

Glycosylphosphatidylinositol (GPI) acyltransferase is crucial for the synthesis of GPI-anchored proteins. Targeting the fungal glycosylphosphatidylinositol acyltransferase GWT1 by manogepix is a promising antifungal strategy. However, the inhibitory mechanism of manogepix remains unclear. Here, we present cryo-EM structures of yeast GWT1 bound to the substrate (palmitoyl-CoA) and inhibitor (manogepix) at 3.3 Å and 3.5 Å, respectively. GWT1 adopts a unique fold with 13 transmembrane (TM) helixes. The palmitoyl-CoA inserts into the chamber among TM4, 5, 6, 7, and 12. The crucial residues (D145 and K155) located on the loop between TM4 and TM5 potentially bind to the GPI precursor, contributing to substrate recognition and catalysis, respectively. The antifungal drug, manogepix, occupies the hydrophobic cavity of the palmitoyl-CoA binding site, suggesting a competitive inhibitory mechanism. Structural analysis of resistance mutations elucidates the drug specificity and selectivity. These findings pave the way for the development of potent and selective antifungal drugs targeting GWT1.

Synergistic inhibition effect and mechanism of an inhibitor for entire process inhibition of coal spontaneous combustion

Traditional coal spontaneous combustion (CSC) inhibitors, while effective, have limitations such as frequent application or short-term efficacy. To this end, we developed a slow-release and water-soluble synergistic inhibitor (SWSI) to achieve long-term CSC inhibition. The SWSI was formulated by integrating synergistic antioxidants (SA) composed of ascorbic acid (AsA) and Fe-superoxide dismutase (Fe-SOD) dissolved in a super absorbent polymer (SAP) encapsulated within hydrogel microcapsules. The effectiveness of SWSI was evaluated through simultaneous thermal analysis and in-situ free radical tests. The formulation optimization demonstrated that AsA and Fe-SOD had the maximum synergistic inhibition effect at a 1:1 molar ratio. The optimal SA: SAP ratio of 3:1 achieved the lowest oxygen consumption rate and highest inhibition rate. The encapsulation formulation, consisting of 3% SA, 6% PAM, 1% CaCl2, and 0.5% citric acid, generated the highest swelling ratio. Simultaneous thermal analysis and in-situ free radical tests indicated that SWSI greatly increased feature temperatures and the apparent activation energy, lowered reaction heat, and substantially reduced free radical concentration in the full oxidation process. The newly developed SWSI offers a significant advancement in long-term CSC prevention by integrating physical and chemical inhibition, which provides sustained and effective inhibition throughout the entire oxidation process.

The Immunomodulatory Mechanisms of BTK Inhibition in CLL and Beyond

Bruton’s tyrosine kinase (BTK), a cytoplasmic tyrosine kinase, plays a pivotal role in B cell biology and function. As an essential component of the B cell receptor (BCR) signaling pathway, BTK is expressed not only in B cells but also in myeloid cells, including monocytes/macrophages, dendritic cells, neutrophils, and mast cells. BTK inhibitors (BTKis) have revolutionized the treatment of chronic lymphocytic leukemia (CLL) and other B cell malignancies. Besides their well-characterized role in inhibiting BCR signaling, BTKis also exert significant immunological influences outside the tumor cell that extend their therapeutic potential and impact on the immune system in different ways. This work elucidates the immunomodulatory mechanisms associated with BTK inhibition, focusing on CLL and other clinical contexts. We discuss how BTK inhibition affects various immune cells, including B cells, T cells, and macrophages. The effects of BTKis on the profiles of cytokines, also fundamental parts of the tumor microenvironment (TME), are summarized here as well. This review also appraises the implications of these immunomodulatory actions in the management of autoimmune diseases and infections. Summarizing the dual role of BTK inhibition in modulating malignant lymphocyte and immune cell functions, this paper highlights the broader potential clinical use of compounds targeting BTK.

Benzoylmesaconine mitigates NLRP3 inflammasome-related diseases by reducing intracellular K+ efflux and disrupting NLRP3 inflammasome assembly

BackgroundBenzoylmesaconine (BMA), a major alkaloid derived from the traditional Chinese medicine Aconitum carmichaeli Debx, exhibits potent anti-inflammatory properties. However, the precise mechanism underlying its action remains unclear. PurposeThis study aimed to investigate the inhibitory mechanism of BMA on the NLRP3 inflammasome and assess its therapeutic efficacy in NLRP3-related metabolic diseases. MethodsA classic NLRP3 inflammasome-activated bone marrow-derived macrophage (BMDM) model was established to evaluate BMA's effects on NLRP3 upstream and downstream protein expression, as well as pyroptosis. Two distinct animal disease models, MSU-induced gouty arthritis and DSS-induced colitis, were utilized to validate BMA's anti-inflammatory activity in vivo. ResultsIn vitro findings revealed that BMA can suppress NLRP3 inflammasome activation by inhibiting interleukin-1β (IL-1β) secretion and GSDMD-N protein expression. This mechanism involved blocking intracellular K+ efflux and interfering with the formation of NLRP3 inflammasomes. In vivo studies demonstrated that BMA significantly alleviated inflammatory symptoms in MSU-induced acute gout and DSS-induced colitis models. ConclusionThese findings suggest that BMA effectively inhibits the activation of the NLRP3 signaling pathway through dual mechanisms: reducing intracellular K+ efflux and disrupting NLRP3 inflammasome assembly. This multifaceted action highlights the therapeutic potential of BMA for NLRP3-related diseases.

Phenazine-1-carboxamide Regulates Pyruvate Dehydrogenase of Phytopathogenic Fungi to Control Tea Leaf Spot Caused by Didymella segeticola.

Due to a lack of understanding of the disease epidemiology and comprehensive control measures, tea leaf spot caused by Didymella segeticola has a significant negative impact on tea yield and quality in the tea plantations of Southwest China. Phenazine-1-carboxamide (PCN) is a phenazine compound derived from Pseudomonas species, which exhibits antimicrobial activity against various pathogens. However, its inhibitory mechanism is not yet clear. The current study evaluated the inhibitory activity of PCN against various phytopathogenic fungi and found that PCN has inhibitory activity against multiple pathogens, with a half-maximal effective concentration (EC50) value for D. segeticola of 16.11 μg/mL in vitro and a maximum in-vivo curative activity of 72.28% toward tea leaf spot. Morphological changes in the hyphae after exposure to PCN were observed through microstructure and ultrastructure analysis, and indicated that PCN causes abnormalities in the hyphae, such as cytoplasmic coagulation, shortened hyphal inter-septum distances, and unclear boundaries of organelles. Transcriptomic analysis revealed that PCN upregulated the expression of genes related with energy metabolism. PCN significantly reduced the ATP concentration in the hyphae and decreased mitochondrial membrane potential. Molecular docking analysis indicated that PCN binds to one of the candidate target proteins, pyruvate dehydrogenase, with lower free energy of -10.7 kcal/mol. This study indicated that PCN can interfere with energy metabolism, reducing ATP generation, ultimately affecting hyphal growth. Overall, PCN shows potential for future application in the control of tea leaf spot due to its excellent antifungal activity and unique mode of action.

Mechanistic in vitro study of the effect of Cucurbita moschata (Cucurbitaceae) on carbohydrate digestive enzymes.

Diabetes is marked by postprandial hyperglycemia (PHG), an abnormal rise in blood glucose after meals. A key therapeutic goal to reduce PHG is the inhibition of α-amylase (αAM) and α-glucosidase (αGL), enzymes that break down carbohydrates into sugars. Cucurbita moschata has been shown to inhibit both enzymes. However, its inhibition mechanism has not been explored. This study investigated the in vitro inhibition mechanisms of αAM and αGL and conducted a metabolomic analysis of C. moschata (edible part) water-extract (CME), aiming to preliminarily identify its bioactive compounds (BCs). The inhibitory mechanisms were determined using Lineweaver-Burk plots. The BCs were identified and quantified using HPLC-QTOF-MS, employing both targeted and untargeted metabolomic approaches. CME had a significant higher effect (p<0.05) on αAM activity than against αGL with IC50 of 28.99 and 698.42mg/mL, respectively. The extract showed mixed and uncompetitive type inhibitions on αAM and αGL, respectively. The lowest inhibition constant (Ki) was 47.68mg/mL on αAM activity at 20mg/mL. Untargeted metabolic profiling by UPLC-MS-ESI-QTOF putatively identified 30 compounds in CME, such as amino acids, vitamins, phytohormones, fatty acids, cucurbitacins and phenolic acids, and flavonoids. Functional analysis of CME identified significant pathways, including pantothenate and CoA biosynthesis and phenylpropanoids, among others. The targeted analysis by UPLC-MS-ESI-QqQ allowed us to identify 12 compounds, with l-phenylalanine, p-hydroxybenzoic, and p-coumaric acid as majors. This study demonstrated the inhibitory potential of CME on αAM and αGL activities, which may be attributed to its metabolites. Thus, this plant represents a valuable source of BC against PHG. Practical Application: The research highlights that Cucurbita moschata has significant potential in managing postprandial hyperglycemia in diabetic patients by inhibiting enzymes like α-amylase and α-glucosidase. In addition, the identification of its compounds emphasizes its importance as a source of bioactive compounds. Therefore, C. moschata could be effectively utilized in the development of nutraceuticals or as an ingredient in functional foods specifically designed for postprandial hyperglycemia management. Thus, integrating C. moschata as part of the daily diet could offer patients with diabetes a natural alternative to control their blood glucose levels after eating.

Deciphering the Impact of Lignin on Anaerobic Digestion: Focus on Inhibition Mechanisms and Methods for Alleviating Inhibition

Deciphering the Impact of Lignin on Anaerobic Digestion: Focus on Inhibition Mechanisms and Methods for Alleviating Inhibition

Hair Growth Effect and the Mechanisms of Rosa rugosa Extract in DHT-Induced Alopecia Mice Model

Rosa rugosa is a medicinal plant known for its potential anti-inflammatory, antioxidant, anti-cancer, and antimicrobial benefits. The pharmacological effects of Rosa rugosa extract on hair loss have not yet been documented. This research sought to assess the inhibitory effects and mechanisms of action of Rosa rugosa water extract (RWE) in a mouse model of dihydrotestosterone (DHT)-induced alopecia. The study was conducted using C57BL/6 mice, which were assigned to five groups: control, DHT-treated, Rosa rugosa water extract (RWE) at doses of 25 mg/kg and 100 mg/kg body weight, and bicalutamide-treated. To induce hair loss, dihydrotestosterone (1 mg/day per body weight) was administered via intraperitoneal injections, and dorsal hair removal was timed to align with the telogen phase. Each group received oral treatments for a period of 23 days. In this study, we assessed hair growth activity, examined histological changes, and performed immunoblot analysis. We noted improvements in hair length and thickness. Additionally, the protein expression of growth factors associated with hair growth, including vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1), showed significant increases in the group treated with RWE. Additionally, treatment with RWE suppressed the protein expression of hair growth inhibitory factors, including dickkopf WNT signaling pathway inhibitor 1 (DKK1) and interleukin (IL)-6. Moreover, hair growth regulatory pathway related factors, including ERK, AKT, and GSK-3β, were activated. These findings indicate that RWE could serve as a promising natural therapy for preventing hair loss by enhancing the production of factors that promote hair growth while inhibiting those that suppress it.

A systematic review of lithium biology and pharmacology and toxicological evaluation of new organic lithium salts

Objective: to systematize scientific data on biomedical studies investigating trace element lithium over the past 70 years; evaluate toxic properties of lithium ascorbate (LiAsc) as an important promising candidate molecule.Material and methods. An analysis of 49,959 publications on lithium biomedical research retrieved from PubMed/MEDLINE database was carried out using modern data mining methods developed within the framework of topological approach to recognizing (Yu.I. Zhuravlev scientific school). Publications found by experts and not indexed in PubMed/MEDLINE were used in discussing the results of a systematic analysis of publications array retrieved from PubMed/MEDLINE. An experimental study of chronic 180 day-long LiAsc (at doses of 5, 50 and 150 mg/kg) toxicity was performed on 36 “Soviet chinchilla” rabbits by assessing local irritant action. Intoxication clinical picture, body weight dynamics, water and food intake as well as physiological, hematological and biochemical parameters were analyzed.Results. Classification and systematization of all currently available publications on lithium biology and medicine were performed. It was shown that pharmacological applications of lithium salts in mental disorders as well as lithium effects on simple sugars metabolism, lipid metabolism, blood pressure regulation, hematopoiesis, inflammation and tumor growth inhibition, neurotransmitter homeostasis, neurotrophic and neuroprotective molecular mechanisms as well as homeostasis of other electrolytes comprised promising fields of lithium drug research. The prospects for using organic lithium salts, particularly LiAsc, for various therapeutic goals were also discussed. 180-day-long oral administration of LiAsc at doses of 5, 50, 150 mg/kg resulted in no macroscopic signs of local inflammatory reaction while examining its local irritant effect.Conclusion. The lithium-ion effect on neurotransmitters promotes neuroprotection and reduces a risk of addiction. The antihypertensive, antiatherosclerotic, antidiabetic, antitumor and neurotrophic effects related to organic lithium salts may be beneficial in various therapeutic applications.

Investigating the inhibitory mechanism of methanogenesis during composting under the combined influence of amoxicillin and copper pollution

This study investigated the effects of different levels of combined amoxicillin and copper (Cu) pollution on the methanogenesis of microbial communities during aerobic composting of dairy manure. Three groups were established: the control group (CK), a low-level combined pollution group (L), and a high-level combined pollution group (H). As the level of pollution increased, carbohydrate metabolism decreased during the thermophilic phase of composting, while signal translation increased. Compared with the initial phase, functional genes related to the acetoclastic pathway decreased significantly in abundance during the thermophilic phase, and cdh had the lowest relative abundance among acetoclastic pathway with a decrease of 81.52%, 81.88%, and 84.73% in groups CK, L, and H, respectively. The cumulative methane emissions in group H decreased by 31.56% and 9.23%, respectively, compared with those from groups CK and L. These results contribute to understanding the effects of combined amoxicillin and Cu pollution on methane emissions during composting.

Injectable and pH-Responsive Metformin-Loaded Hydrogel for Active Inhibition of Posterior Capsular Opacification.

Posterior capsular opacification (PCO) is a common complication following cataract surgery, which can lead to a significant vision loss. This study introduces a facile method for developing a metformin-derived hydrogel (HCM6) stabilized by dynamic covalent bonds among natural polymers. This hydrogel demonstrates antifibrotic properties, on-demand drug release, pH responsiveness, injectability, and self-healing capabilities. Our in vitro experiments confirmed that the HCM6 hydrogel exhibits excellent biocompatibility, inhibiting lens epithelial cell migration, and transforming growth factor-2β (TGFβ2)-induced α-smooth muscle actin (α-SMA) expression in lens epithelial cells. In vivo studies conducted in a rat extracapsular lens extraction (ECLE) model revealed that HCM6 significantly suppressed PCO after 21 days of implantation with no observed pathological effects on surrounding tissues or the optic nerve. According to our experimental results, the inhibitory mechanism of PCO may be attributed to metformin's suppressive effect on lens cell migration, epithelial-mesenchymal transition (EMT), and lens fiber formation. In summary, the long-acting, controllable, and on-demand release characteristics of the HCM6 hydrogel not only provide an effective strategy for preventing PCO but also offer new avenues for treating undesirable proliferative conditions in ophthalmology and beyond.

The characteristics and mechanism of NaHCO3 compound fly ash in suppressing gas explosions in pipeline networks

Gas has been widely concerned due to its importance in the industrial and energy fields. In order to improve the inhibition effect of gas explosion, under the self-constructed pipe network system, the inhibition of gas explosion by composite inhibitor of fly ash and NaHCO3 with different ratios was investigated, and the microscopic inhibition mechanism of the two kinds of powders on the explosion of gas was investigated based on the method of density functional theory and transition state theory from the perspective of molecular dynamics. The results show that: the composite powder explosion suppression is better than a single powder, explosion suppression effect with the increase in the proportion of the mass of NaHCO3 significantly improve the 5 groups of conditions NaHCO3 loading of 40% (by mass) is the best, this time, the explosion of the peak overpressure, the peak flame propagation velocity, the peak flame temperature compared to the maximum reduction in the percentage of the measures taken for the maximum of 74.42%, 81.93%, 68.71%. In addition, NaHCO3, fly ash effectively inhibit the key primitive reaction of gas explosion. When the temperature reaches a certain point, the two and O*, O2, OH* and H* reaction rate higher than the rate of CH4 oxidation chain reaction, the dominant reaction, the maximum difference in rate constants of 70.95, 58.81, 60.06, 44.94. The study of the transition from the macro-scale to the molecular level of the study, in-depth understanding of the mechanism of explosion suppression, to reduce the risk of explosion from the ground up, and enriches the Gas explosion prevention and control of the theoretical system, for the operators to provide a strong technical guarantee for safe production.

Lexical inhibition after semantic violations recruits a domain-general inhibitory control mechanism.

Language processing is incremental. As language signals-for example, words in a sentence-unfold, humans predict and activate likely upcoming input to facilitate comprehension. Prediction not only accelerates understanding but also prompts reassessment in the case of prediction error, fostering learning and refining comprehension skills. Therefore, it is paramount to understand what happens when linguistic predictions are violated-for example, when a sentence ends in an unpredicted word. One theory, which we test here, is that the originally predicted word is actively inhibited after semantic violations. Furthermore, we tested whether this purported lexical inhibition process is achieved by a domain-general mechanism-that is, one that also inhibits other processes (e.g., movement). We combined a semantic violation task, in which highly constrained sentences primed specific words but sometimes continued otherwise, with a motoric stop-signal task. Across two experiments, semantic violations significantly impaired simultaneous action-stopping. This implies that lexical and motor inhibition share the same process. In support of this view, multivariate decoding of electroencephalographic recordings showed early overlap in neural processing between action-stopping (motor inhibition) and semantic violations (lexical inhibition). Moreover, a known signature of motor inhibition (the stop-signal P3) was reduced after this initial overlap period, further suggesting the presence of a bottleneck due to shared processing. These findings show that semantic violations trigger inhibitory processing and suggest that this lexical inhibition recruits a domain-general inhibitory control mechanism. This provides a new perspective on long-standing debates in psycholinguistics, extends the range of a well-characterized cognitive control mechanism into the linguistic domain, and offers support for recent neurobiological models of domain-general inhibitory control. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

The impact of minocycline in inhibiting glial scar formation in rats with traumatic brain injury: A mini scoping review

Minocycline, a second-generation tetracycline derivative, is proven to inhibit glial scar formation in several neurological diseases. However, studies associating micocycline in traumatic brain injury (TBI) is very limited. This review aims to determine the role of minocycline in inhibiting glial scar or astrogliosis formation in TBI animal models. This scoping review includes original studies in PubMed, Science Direct, and Google Scholar databases published between 1 January 2012 to 31 December 2022, in full text, involving rodent research, and written in English. Two authors who followed the Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR) guidelines, conducted the assessment independently. Of 2687 studies, 13 studies are reviewed. Two studies describe the benefits of minocycline in inhibiting glial scar formation in TBI, while 11 studies show that minocycline inhibits glial scar formation in diseases other than TBI. An explanation of the signaling pathways and cells involved in the mechanism of glial scar inhibition by minocycline can be found in ten articles, of which four observe the role of microglial cells, four observe the role of astrocyte cells, and two do not explain the mechanism. Research on the impact of minocycline in inhibiting glial scar formation in rats with TBI is limited. The results of this review support early research on the role of minocycline in inhibiting glial scar or astrogliosis in TBI, and similar studies in several other CNS diseases support this. However, the mechanism of minocycline's inhibitory pathway to glial scarring remains unclear.

Exploring the Experimental and Theoretical Studies and Inhibition Mechanism of Passiflora Incarnata Extract as a Novel Green Inhibitor for API 5CT N80 in an Aggressive Environment

Exploring the Experimental and Theoretical Studies and Inhibition Mechanism of Passiflora Incarnata Extract as a Novel Green Inhibitor for API 5CT N80 in an Aggressive Environment

Mechanism of four dihydroflavones inhibiting heterocyclic amine formation in roasted pork patties―increasing the moisture

This study explored the inhibitory effect and mechanisms of four dihydroflavones (hesperetin, hesperidin, naringenin and naringin) addition on the production of five HCAs (IQ, DMIP, MeIQx, Norharman and PhIP) in roasted pork patties. Results indicated that the moisture holding capacity of dihydroflavones played a crucial role in their inhibition on HCA formation. The importance of moisture in the HCA formation was further confirmed by adding two water-retaining edible starches (corn and sweet potato starch). Significantly, the combination of edible starch and naringenin resulted in better inhibition of HCAs. The inhibition ratio of the mixture containing corn starch and naringenin on IQ, DMIP, MeIQx, Norharman and PhIP of pork patties were even up to 76.64%, 100%, 53.29%, 91.29% and 100%, respectively. This study offers a new idea of combination of dihydroflavone and edible starch to better control the HCA formation in roasted meat products.

Synergistic inhibition effect of Chlorella sp. and benzotriazole on the corrosion of Q235 carbon steel in alkaline artificial seawater

The interaction of microalgae on the reinforced concrete with corrosion inhibitor is not well understood. Moreover, the inhibition role of microalgae on corrosion has been reported in recent years. In this study, the corrosion inhibition behavior of Q235 carbon steel (CS) due to the presence of Chlorella sp. and benzotriazole (BTA) in alkaline artificial seawater was investigated by means of weight loss, electrochemical measurements including open circuit potential, electrochemical impedance spectroscopy, and potentiodynamic polarization curves, and surface analysis including scanning electron microscopy, energy dispersion spectrometer, and X-ray photoelectron spectroscopy. The results of reduced corrosion rates in the algae-BTA system demonstrated that Chlorella sp. could facilitate the corrosion inhibition efficiency of BTA on the CS specimens. Moreover, polarization measurement showed the algae-BTA system had a mixed-type corrosion inhibition effect. The mechanisms of inhibition were proposed to be the precipitation of iron complexes such as Fe-BTA-EPS and Fe-BTA and iron compounds on the steel surface in the presence of the microalgae and BTA–. This study highlights the application of CS corrosion control by combining biological and chemical approaches that can be used for future research and practice, rather than purely chemical approaches.

Potential Antitumor Mechanism of Propolis Against Skin Squamous Cell Carcinoma A431 Cells Based on Untargeted Metabolomics.

Propolis is a sticky substance produced by honeybees (Apis mellifera) through the collection of plant resins, which they mix with secretions from their palate and wax glands. Propolis can inhibit tumor invasion and metastasis, thereby reducing the proliferation of tumor cells and inducing cell apoptosis. Previous research has shown that propolis has an inhibitory effect on skin squamous cell carcinoma A431 cells. Nevertheless, its inhibitory mechanism is unclear because of many significantly different Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways between the ethanol extract of the propolis (EEP) group and the control group of cells. In this study, the main components of EEP and the antitumor mechanism at an IC50 of 29.04 μg/mL EEP were determined via untargeted metabolomics determined using ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC‒MS/MS), respectively. The results revealed 43 polyphenolic components in the EEP and 1052 metabolites, with 160 significantly upregulated and 143 significantly downregulated metabolites between cells treated with EEP and solvent. The KEGG enrichment results revealed that EEP significantly inhibited A431 cell proliferation via the steroid hormone biosynthesis and linoleic acid metabolism pathways. These findings may provide valuable insights for the development of targeted therapies for the treatment of cutaneous squamous cell carcinoma.

Theta oscillations in observers’ temporal cortex index postural instability of point-light displays

This study investigates whether postural equilibration follows the same principles of motor resonance as goal-oriented actions, namely, whether an individual activates the same neuronal substrates when experiencing postural perturbation as when observing another individual in this condition. To address this question, we examined electroencephalographic dynamics while subjects observed point-light displays featuring an unstable human display, a stable human display, and their respective scrambled counterparts lacking shape information and biological motion. We focused on theta band (4–7 Hz), which is a fundamental frequency for modulating brain activity during challenging balance tasks and reflects postural stability monitoring. Rather than mirroring activity, our findings suggest an inhibitory response to postural instability. Theta event-related synchronization in the left temporal cortex was dampened for the unstable display as compared to its scramble counterpart and to the stable display. This low theta response coincided with an increase in left temporal-prefrontal connectivity, compatible with top-down inhibitory mechanisms. By contrast, the stronger theta response to the stable display as compared to the unstable one could be due to the difficulty of recognizing low-motion biological stimuli, or alternatively, to a facilitation of stimulus processing and strengthening of the mirroring response. The response facilitation for stable posture, coupled with a diminished response to the unstable display, could contribute to a broader mechanism mitigating postural threats and ensuring stable balance. Future investigations should leverage these findings to explore how posture-related responses correlate with perceptual and motor expertise, and to more clearly define these mechanisms during dynamic social interactions.

Evaluation of the synergic effect of amino acids for CO2 hydrate formation and dissociation

Gas hydrate formation is a very challenging problem in the oil and gas industry. The chemical inhibition method is the best practicable hydrate mitigation method by injecting hydrate inhibitors such as Thermodynamic Hydrate Inhibitors (THIs) and Kinetic Hydrate Inhibitors (KHIs) into the pipeline. However, the hydrate inhibitors' biodegradability and limited data on dual functional inhibitor mixtures raise concerns in this study. This study aims to investigate and compare thermodynamic and kinetic inhibition on CO2 hydrate by using biodegradable amino acid inhibitor mixtures of Glycine and Alanine. In this study, the inhibitor mixtures were prepared by mixing Glycine and Alanine in 3 different mixture ratios, followed by the Thermodynamic and Kinetic inhibition tests. Thermodynamic Inhibition Test was conducted by using 10 wt% of inhibitor mixtures in a CO2-water system under pressure of 4.0, 3.5, 3.0 and 2.5 MPa, while Kinetic Inhibition Test was carried out by using 1 wt% of the sample in the CO2-water system at a constant pressure of 4.0 MPa and temperatures at 274.15K. The analysis methods for the Thermodynamic Inhibition Test were Hydrate Liquid-Vapor Equilibrium (HLVE) Curve, Average Depression Temperature and Molar Dissociation Enthalpy, while the Induction Time method, the number of CO2 moles consumed, the formation rate of CO2 gas hydrate and RIP were used in the Kinetic Inhibition Test. In this study, all inhibitor mixtures exhibit dual functional inhibition on the CO2 hydrate. For THI, 50% Gly: 50% Ala shows the best thermodynamic inhibition strength with an average depression temperature of 2.42 K. All the inhibitor mixtures for THI have the dissociation enthalpies within the CO2 hydrate enthalpy range, which show that the inhibition is due to the influence on the water molecules’ interaction only. For KHI, 75% Gly: 25% Ala shows the highest kinetic inhibition strength on CO2 formation with the longest induction time of 115.2 min and the highest RIP of 0.40. In the KHI experiment, there is a drop in the CO2 uptake capacity with the presence of inhibitors used, where the highest difference of CO2 moles consumed is 0.0057 mol. A synergistic effect of inhibitor mixtures was observed in this study on 50% Gly: 50% Ala for THI and 75% Gly: 25% Ala for KHI. However, the “degradation” effect was also exhibited on 75% Gly: 25% Ala and 25% Gly: 75% Ala for THI and 50% Gly: 50% Ala and 25% Gly: 75% Ala for KHI, where the performance of the inhibitor mixtures is poorer than the concentrated amino acids. The “degradation” effect of the inhibitor mixtures at certain ratios was not covered in this study and it requires a further understanding of the chemistry behind the inhibition mechanism.