People pay more and more attention to sports and health, especially the goal of sports recovery and scientific sports is more and more clear. In order to avoid human injury caused by unreasonable sports, it is necessary to carry out real-time detection of human motion state. In this paper, the motion state of human body was taken as the test object, a flexible strain sensitive material combining iron nanowires and carbon microspheres was proposed, and polyester fabric was introduced as the sensor substrate to construct a sandwich structure flexible fabric strain sensor. Then a mechanical test platform was built to carry out mechanical property testing and practical application verification research. The mechanical test results show that the iron nanowires/carbon microspheres flexible fabric strain sensor has good linearity, the response time is 42 ms, and the hysteresis error is approximately 8.7 %. Finally, the flexible fabric strain sensor is fixed on the part of the human body that needs to be detected, and the electrical signal curve of the response is output by the electrochemical workstation. Through the analysis of the response curve, it can be seen that the iron nanowires/carbon microspheres flexible fabric strain sensor prepared in this paper has great potential in human movement health monitoring.