Mechanical Properties Of Human Skin Research Articles

Tribological method to objectify similarity of vague tactile sensations experienced during application of liquid cosmetic foundations

To develop objective estimation methods for the tactile sensations experienced during the application of liquid cosmetic foundation (i.e., emulsion foundations (EFs)), a tribotester based on the mechanical properties of human skin was used in lubrication experiments with EF samples. From the time-varying signals of the tangential spring deflection obtained in reciprocating rubbing tests for small sample amounts, trajectories (i.e., plots of the tangential spring deflection against the tangential drive position) were obtained. Using characteristic trajectory shapes (e.g., a shape like a ribbon with jagged edges) makes it possible to evaluate the similarity of vague tactile sensations without sensory testing.

Non contact method for in vivo assessment of skin mechanical properties for assessing effect of ageing

The assessment of human tissue properties by objective and quantitative devices is very important to improve the understanding of its mechanical behaviour. The aim of this paper is to present a non contact method to measure the mechanical properties of human skin in vivo.A complete non contact device using an air flow system has been developed. Validation and assessment of the method have been performed on inert visco-elastic material. An in vivo study on the forearm of two groups of healthy women aged of 23.2±1.6 and 60.4±2.4 has been performed. Main parameters assessed are presented and a first interpretation to evaluate the reduced Young's modulus is proposed.Significant differences between the main parameters of the curve are shown with ageing. As tests were performed with different loads, the influence of the stress is also observed. We found a reduced Young's modulus with an air flow force of 10mN of 14.38±3.61kPa for the youngest group and 6.20±1.45kPa for the oldest group. These values agree with other studies using classical or dynamic indentation. Non contact test using the developed device gives convincing results.

Quantifying the mechanical properties of human skin to optimise future microneedle device design

Microneedle devices are a promising minimally invasive means of delivering drugs/vaccines across or into the skin. However, there is currently a diversity of microneedle designs and application methods that have, primarily, been intuitively developed by the research community. To enable the rational design of optimised microneedle devices, a greater understanding of human skin biomechanics under small deformations is required. This study aims to develop a representative stratified model of human skin, informed by in vivo data. A multilayer finite element model incorporating the epidermis, dermis and hypodermis was established. This was correlated with a series of in-vivo indentation measurements, and the Ogden material coefficients were optimised using a material parameter extraction algorithm. The finite element simulation was subsequently used to model microneedle application to human skin before penetration and was validated by comparing these predictions with the in-vivo measurements. Our model has provided an excellent tool to predict micron-scale human skin deformation in vivo and is currently being used to inform optimised microneedle designs.

A new stochastic inverse identification of the mechanical properties of human skin

The study of the mechanical properties of human skin is a key point to better understand surgery, skin ageing and pathologies. As the skin is a living tissue, it must be studied in vivo, hence analytical solutions are really difficult to obtain. In this study, a new stochastic inverse method for the identification of its mechanical properties is proposed. The developed optimization method is first presented. It is based on an iterative stochastic approach which ensures the identification of a global extremum. The suction actual case study is then analysed through comparisons between experimental data and finite element models of this test. Only the elastic components of the skin are considered here. The solutions for the recursive least squares and Gauss-Newton's problems are finally compared with the proposed approach to conclude this study and to briefly present our future works.

Application of substrate curvature method to differentiate drying stresses in topical coatings and human stratum corneum

Despite the extensive use of topical coatings in cosmetics, their effect on the mechanical properties of human skin and the perception of skin tightness in the form of drying stresses is not well understood. We describe the application of a recently developed substrate curvature technique to characterize stresses in drying and non-drying occlusive topical coatings. We then extend the technique to measure the combined effects of the coating applied to human stratum corneum (SC) where the overall drying stresses may have contributions from the coating, the SC and the interaction of the coating with the SC. We show how these separate contributions in the coating and SC layers can be differentiated.

Skin–textile friction and skin elasticity in young and aged persons

The mechanical properties of human skin are known to change with ageing, rendering skin less resistant to friction and shear forces, as well as more vulnerable to wounds. Until now, only few and contradictory results on the age-dependent friction properties of skin have been reported. This study has investigated in detail the influence of age on the friction of human skin against textiles. In vivo skin-friction measurements on a force plate were combined with skin analyses concerning elasticity, hydration, pH value and sebum content. Thirty-two young and 28 aged persons rubbed their volar forearm in a reciprocating motion against various textiles on the force plate, using defined normal loads and sliding velocities, representing clinically relevant contact conditions. Mean friction coefficients ranged from 0.30 +/- 0.04 (polytetrafluoroethylene) to 0.43 +/- 0.04 (cotton/polyester). No significant differences in the friction properties of skin were found between the age groups despite skin elasticity being significantly lower in the aged persons. Skin hydration was significantly higher in the elderly, whereas no significant differences were observed in either skin pH value or sebum content. Adhesion is usually assumed to be the dominant factor in skin friction, but our observations imply that deformation is also an important factor in the friction of aged skin. In the elderly, lower skin elasticity and skin turgor are associated with more pronounced skin tissue displacements and greater shear forces during frictional contact, emphasizing the importance of friction reduction in wound-prevention programmes.

Measuring the mechanical properties of human skin in vivo using digital image correlation and finite element modelling

The mechanical properties of the skin are important in many applications, but are not well understood. This paper presents a method for measuring the mechanical properties of human skin in vivo using digital image correlation, with a finite element model that was used to optimize the material properties to obtain the best match with the model data. The skin was modelled as an Ogden hyperelastic membrane, with a tension field wrinkling model and an initial stretch identified as an additional material parameter, and the boundary conditions were the measured load and the displacements around the edge of the region of interest. Fast, reliable convergence was obtained using a Hager–Zhang non-linear conjugate gradient solver. A stochastic optimization procedure was used to identify the material parameters. Good estimates of the material parameters could be obtained from the displacement field at a single time point. Typical material parameters were μ = 10 Pa, α = 26, and an initial strain of 0.2. These parameters were not unique; the stochastic optimization procedure gave good global convergence and an indication of the overall uncertainty in the identification of the results. It is argued that the use of the DIC technique, which generates very large amounts of data, also gave a clearer picture of the overall uncertainty.

Use of the Kalman filters for the analysis of the mechanical properties of human skin in vivo

In this article, we present an inverse method for the identification of the mechanical properties of human skin, a complex multi-layered organ which has been studied in vivo using a suction deformation technique. To identify the required properties, experimental results were compared to finite element solutions of the test, with the assumption that skin behaves as a single isotropic hyperelastic layer. The inverse method used in this article is based on that of the extended Kalman filters principle, with two modifications of this standard formulation for use in skin analysis. The modified formulation was then tested using Finite Element Method (FEM) simulated mechanical data and also with a study of linear and nonlinear theoretical problems. The results of the new formulation were also compared with the Gauss–Newton, recursive least square and Kalman smoother approaches. Finally, the reliability of the method was tested on a case study.

New extensometer to measure in vivo uniaxial mechanical properties of human skin

Biomechanical properties of skin are important for clinical decision making as well as clinical intervention. Measuring these properties in vivo is critical for estimating dimensional behaviour of skin flap or graft after harvest. However, existing methodologies and devices often suffer from lack of standardisation and unwanted peripheral force contribution due to the deformation of surrounding tissues during measurement. This naturally leads to measurement inaccuracies and lack of reproducibility. In order to improve the measurement accuracy, a new portable extensometer, which measures the non-invasive in vivo biomechanical properties of skin, has been designed and constructed. This design incorporates three pads that attach to the skin, including a C-shaped pad to shield the force sensor from peripheral forces. Such design produces data that are significantly closer to in vitro measurements. The results have been verified by finite element analysis, and experiments on rubber sheets and pig skins. This device can be used to obtain biomechanical properties of skin that will aid doctors in measuring skin elasticity and surgical planning, especially in skin flap surgery.

In vivo characterization of the mechanical properties of human skin derived from MRI and indentation techniques

The human skin is an exceedingly complex and multi-layered material. This paper aims to introduce the application of the finite element analysis (FEA) to the in vivo characterization of the non-linear mechanical behaviour of three human skin layers. Indentation tests combined with magnetic resonance imaging (MRI) technique have been performed on the left dorsal forearm of a young man in order to reveal the mechanical behaviour of all skin layers. Using MRI images processing and a pre and post processor allows to make numerically individualized 2D model which consists of three skin layers and the muscles. FEA has been applied to simulate indentation tests. Neo-Hookean slightly compressible material model of two material constants (C10, K) has been used to model the mechanical behaviour of the three skin layers and the muscles. The identification of material model parameters was done by applying Levenberg–Marquardt algorithm (LMA). Our methodology of identification provides a range of values for each constant. Range of values of different material properties of epidermis, dermis, hypodermis are respectively, C10E = 0.12 ± 0.06 MPa, C10D = 1.11 ± 0.09 MPa, C10H = 0.42 ± 0.05 KPa, K E = 5.45 ± 1.7 MPa, K D = 29.6 ± 1,28 MPa, K H = 36.0 ± O.9 KPa.

In vivo measurements of the elastic mechanical properties of human skin by indentation tests

Knowledge about the human skin mechanical properties is essential in several domains, particularly for dermatology, cosmetic or to detect some cutaneous pathology. This study proposes a new method to determine the human skin mechanical properties in vivo using the indentation test. Usually, the skin mechanical parameters obtained with this method are influenced by the mechanical properties of the subcutaneous layers, like muscles. In this study, different mechanical models were used to evaluate the effect of the subcutaneous layers on the measurements and to extract the skin elastic properties from the global mechanical response. The obtained results demonstrate that it is necessary to take into account the effect of the subcutaneous layers to correctly estimate the skin Young's modulus. Moreover, the results illustrate that the variation of the measured Young's modulus at low penetration depth cannot be correctly described with usual one-layer mechanical models. Thus a two-layer elastic model was proposed, which highly improved the measurement of the skin mechanical properties.

The in vivo characterization of the mechanical properties of human skin by MRI and indentation techniques

The in vivo characterization of the mechanical properties of human skin by MRI and indentation techniques

An inverse identification of the mechanical properties of human skin

The mechanical properties of the skin are of potential interest in the identification of certain diseases, for assessing therapeutic intervention, or for predicting the effect of a trauma. Such an ...

Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study

Extracellular matrix (ECM) organization is a complex process that requires the coordinated efforts of many molecules. For the regulation of collagen fiber diameter, the proteoglycan decorin appears to be of major relevance. To investigate the role of decorin in the process of (photo-)aging in more detail, full-thickness punch biopsies were isolated from human buttock skin. Single exposure with two minimal erythemal doses of solar simulated irradiation caused down-regulation of decorin mRNA in young (n = 5) and old subjects (n = 5) after 24 h. Interestingly, decorin mRNA was elevated with age. To test the hypothesis that a decreased collagen-to-decorin-ratio impairs collagen structure we also investigated collagens I and III gene expression. Both were down-regulated with increasing age and after single UV-irradiation. As determined by laser capture microdissection-quantitative real time-Polymerase chain reaction (n = 11), decorin is mostly present in the reticular dermis while being absent from the papillary dermis. Minor expression was also observed in the epidermis. However, in contrast to full-thickness skin biopsies age-dependent changes in collagens I, III, and decorin expression could not be observed with this methodology indicating technical limitations. Together with our finding that collagens I and III mRNA are similarly expressed in the reticular and papillary dermis and are down-regulated by UV, our studies support the idea of a major role of decorin in ECM organization. Altered expression of decorin mRNA in the different dermal strata and a decrease in the collagen-to-decorin ratio inflicted by both age and ultraviolet irradiation possibly affect collagen bundle diameter and subsequently the mechanical properties of human skin.

Variations with age in the mechanical properties of human skin in vivo

To quantitatively assess the viscoelastic properties of human skin, an in vivo testing method has been developed and previously reported. In this method, a strain-gauged pretension device is first used to determine the natural skin tension and then to apply a predetermined tension. A suction cup device with a rectangular cross-section and semi-circular ends is then applied to the pretensioned area and the skin deflection versus negative pressure characteristic is recorded. With the basic characteristics of the skin stress field determined by the geometry of the cup as a homogeneous stress state, the stress-strain response curve is computed from the recorded data. Using a constant pressurisation rate testing mode, the back and forearm of 116 healthy male and female subjects ranging in age from two to 67 years were tested. Variations in the shape and average slope (approximate modulus) of the stress-strain curves have been investigated with changes in age and sex. It has been found that the average slope decreases through maturation, reaches a minimum between the ages of 15 and 25, and then appears to increase with advancing age. The data points begin to diverge after approximately 30 years of age. It is hypothesised that this diverging phenomenon may be a result of the cumulative effect of ultraviolet radiation on the collagen and elastin networks. An examination of the shape of the curves appears to indicate that stiffness of collagen in the dermis increases with increasing age. However, it appears that the lower portions of the curves, possibly associated with the elastin and ground substance components, undergo changes not common to every individual. It is also hypothesised that 'scatter' in the data obtained from females and the sudden change noted in the shape of the curves at puberty may be due to hormonal changes.

Measurement of the mechanical properties of the skin using the suction test

The suction test is commonly used to study the mechanical properties of human skin in vivo. The unevenness of the stress fields complicates obtaining the intrinsic mechanical parameters of the skin in vivo because the values of the local stresses and deformations cannot be calculated directly from the displacements and forces applied by the test apparatus. In general, users only take into account the negative pressure applied and the elevation of the dome of skin drawn up in order to deduce the properties of the skin. This method has the major disadvantage of being dependent on the experimental conditions used: in particular, the size of the suction cup and the negative pressure applied. Here, we propose a full mechanical study of the test to provide rigorous results. We compare the frequently used geometric method (making the thin plate hypothesis), Timoshenko's method (which can take greater plate thicknesses into account) and finally various results obtained by the finite elements (FE) technique. The suction test was modelled by FE with large displacements and large deformations both for orthotropic and isotropic plates. The results obtained in the elastic domain for various values of Young's modulus and of applied negative pressure were used as references and were compared with methods using analytical relationships. The geometric method generally used in the interpretation of suction tests gives results, which in certain configurations, are very different from those obtained by FE. The method of Timoshenko is suited to thick plates 'in contact' or embedded round the edge, the elevation of the dome and the tension and flexion stresss are analytically accessible through relationships involving four constants that are dependent on the limit conditions. Comparison with the FE results enabled the optimisation of the coefficients to adapt the relationships to the particular conditions of the suction trials. We showed the limits of the geometrical method and proposed a solution, which while remaining simple to use, gives results that are closer to reality both for the calculation of the modulus and for the determination of the state of the stresses obtained.

Finite element model of mechanical properties of human skin in vivo under indentation coupled with MRI technique

Finite element model of mechanical properties of human skin in vivo under indentation coupled with MRI technique

Characterization of the mechanical properties of skin by inverse analysis combined with the indentation test

This study proposes a new method to determine the mechanical properties of human skin by the use of the indentation test [Pailler-Mattei, 2004. Caractérisation mécanique et tribologique de la peau humaine in vivo, Ph.D. Thesis, ECL-no. 2004-31; Pailler-Mattei, Zahouani, 2004. Journal of Adhesion Science and Technology 18, 1739–1758]. The principle of the measurements consists in applying an in vivo compressive stress [Zhang et al., 1994. Proceedings of the Institution of Mechanical Engineers 208, 217–222; Bosboom et al., 2001. Journal of Biomechanics 34, 1365–1368; Oomens et al., 1984. Selected Proceedings of Meetings of European Society of Biomechanics, pp. 227–232; Oomens et al., 1987. Journal of Biomechanics 20(9), 877–885] on the skin tissue of an individual's forearm. These measurements show an increase in the normal contact force as a function of the indentation depth. The interpretation of such results usually requires a long and tedious phenomenological study. We propose a new method to determine the mechanical parameters which control the response of skin tissue. This method is threefold: experimental, numerical, and comparative. It consists combining experimental results with a numerical finite elements model in order to find out the required parameters. This process uses a scheme of extended Kalman filters (EKF) [Gu et al., 2003. Materials Science and Engineering A345, 223–233; Nakamura et al., 2000. Acta Mater 48, 4293–4306; Leustean and Rosu, 2003. Certifying Kalman filters. RIACS Technical Report 03.02, 27pp. http://gureni.cs.uiuc.edu/~grosu/download/luta+leo.pdf; Welch and Bishop, An introduction to Kalman filter, University of North Carolina at Chapel Hill, 16p. http://www.cs.unc.edu/~welch/kalman/]. The first results presented in this study correspond to a simplified numerical modeling of the global system. The skin is assumed to be a semi-infinite layer with an isotropic linear elastic mechanical behavior [Zhang et al., 1994. Proceedings of the Institution of Mechanical Engineers 208, 217–222] This analysis will be extended to more realistic models in further works.

In vivo experimental testing and model identification of human scalp skin

A comprehensive experimental/numerical procedure is formulated and validated for the in vivo characterization of the mechanical properties of human skin and the simulation of reconstructive surgery. The procedure uses in vivo experimental tests on undermined skin flaps, which can be performed during surgery, a numerical model formulated within the framework of nonlinear finite strain elasticity and a nonlinear parameter identification technique for the calibration of the model from indirect measurements. The procedure is applied to characterize the scalp skin tested in Raposio and Nordström (Skin Res. Technol. 4 (1998) 94). The skin is treated as a time independent, isotropic and hyperelastic membrane and the problem is solved through a finite element discretization. The study highlights that the model parameters can be determined with good accuracy using displacement measurements of a few points in the domain.

Model of the viscoelastic behaviour of skin in vivo and study of anisotropy

The single-axis extension test is relatively little used to study the mechanical properties of human skin in vivo. A campaign of tests was carried out with an original, modern machine developed in our laboratory. It can perform extension or compression tests using servo-controlled position or force in different directions. The load can either be of the extension or monotonous compression type, creep or relaxation. The results obtained were used to develop a viscoelastic model. The elastic modulus calculated helps us to determine the main directions of anisotropy on the forearm. We use a new in vivo single-axis extension machine (patent no. FR03/09220 application in progress). With it, we can carry out monotonous, creep and relaxation tests on the forearm. An associated finite elements model enables conversion to the intrinsic parameters of the skin under stress and strain from external stress applied in force and displacement. From the tests, we can propose a viscoelastic model and the identification of his parameters. We carried out tests in four directions with respect to the axis of the forearm of 63 people of different ages. The present report is limited to a brief presentation of the experimental set-up used, and a more complete presentation of the viscoelastic model and how it is defined and also the work on the anisotropy in the elastic domain. The viscoelastic model proposed has only four intrinsic parameters: elasticity parameters E(e) and E(ve) and viscosity parameters epsilon(ve) and A. Skin being considered as orthotropic, we were able to determine the average main direction of 63 people, which is of 5.33+/-5.78 around the longitudinal axis of the arm. An average modulus E(1) (ave)=6.57E(5) (Pa) can be found in the direction close to the axis of the arm and E(2) (ave)=1.30E(5) (Pa) in the perpendicular direction and a G(12)=1.32E(5) (Pa) shear modulus. The parameters obtained with the viscoelastic model are independent of the type of load, the same coefficients enable a correct representation in creep and relaxation tests. The main directions vary from one person to another, Young's modulus in these directions could be an indicator for dermatologists and cosmeticians.