Mechanism Of Modification Research Articles

Investigation into the modification mechanism and performance assessment of waste battery powder modified bitumen

ABSTRACT In order to reduce the impact of Waste Battery on the environment, this study mixed Waste Battery Powder (WBP) into bitumen to make waste battery modified bitumen. The compatibility and action mechanism of WBP modified bitumen have been investigated by molecular dynamics, the influence of WBP on bitumen aggregation and asphalt molecular movement has been analysed, the interaction energy between WBP and bitumen has been evaluated, and the cohesion energy density and modulus of WBP bitumen have been studied. The density of polar functional groups in WBP is high, and the interaction between WBP and other molecules is enhanced by numerous benzene ring structures, which can effectively adsorb the light components of bitumen. MnO2 and other metal components have strong electrostatic attraction with bitumen molecules, which makes the interface adhesion between WBP and bitumen phase excellent, resulting in a stronger resistance to shear deformation of WBP modified bitumen. The results show that the addition of WBP can improve the resistance to load deformation and shear resistance, and improve the high temperature stability. This study provides a theoretical basis for reducing the pressure of waste batteries on the environment and further studying the use of waste batteries for modified bitumen.

Fabricating ultra wear-resistant surfaces on titanium alloy by combining laser-induced modification with abrasive belt grinding

A novel method combining laser-induced modification and abrasive belt grinding (LM&BG) was proposed to prepare wear-resistant surfaces of titanium alloys. Comprehensively investigated the evolution mechanism of material modification and wear resistance of LM&BG samples. It was found that an oxide-ceramic-modified layer with increasing hardness (10 times) was formed by laser modification on the titanium alloy, mainly composed of TiO2, with the two phases Rutile-TiO2 and Anatase-TiO2. Moreover, the surface roughness after abrasive belt grinding of the modified layer is less than Ra0.1, with a uniform subsurface microstructure. Interestingly, the wear modes of the modified layer shifted from adhesive and oxidative wear to brittle microdetachment, significantly reducing the coefficient of friction, material wear, and subsurface damage, thus improving the wear-resistant capability.

Study on the high-strength and high-conductivity modification mechanisms of C7035 copper alloy induced by La and Sc doping

The effects of multi-element doping on mechanical and electrical properties of C7035 copper alloy were investigated by first principles calculation and experiment based on density functional theory (DFT). The results show that the addition of lanthanum (La) significantly improves the electrical conductivity of copper alloy, mainly because the precipitated phase of lanthanum (La) has high electrical conductivity, and promotes the formation of high-density twins in 0 Cu matrix. The electrical conductivity of the C7035 alloy doped with La reaches 80.19 % of IACS, which is 44.17 % higher than that of the alloy without La. In addition, the incorporation of scandium (Sc) increased the yield strength of the alloy to 635.63 MPa, an improvement of 25.65 %, due to the excellent mechanical properties of the ScCu₄ precipitated phase. The highly conductive NiSi precipitated phase is formed by the copper alloy doped with La and Sc simultaneously. Compared with the undoped alloy, the yield strength and conductivity of the co-doped alloy are 672.27 MPa, 62.7 %, 32.89 % and 12.73 % respectively. In summary, the coupling doping of La and Sc improves the mechanical and electrical properties of C7035 copper alloy, which provides theoretical guidance for the design of high-strength and high-conductivity copper alloys.

Polyethylene and related hydrocarbon polymers (“plastics”) are not biodegradable

Research on the biodegradation of polyethylene (PE), polystyrene (PS) and related polymers has become popular and the number of publications on this topic is rapidly increasing. However, there is no convincing evidence that the frequently claimed biodegradability of these so-called “plastics” really exists. Rather, a diffuse definition of the term “biodegradability” has led to the publication of reports showing either marginal weight losses of hydrocarbon polymers by the action of isolated bacterial strains or mechanical disintegration and polymer surface modification in case of hydrocarbon polymer-consuming insect larvae. Most of the data can be alternatively explained by the utilization of polymer impurities/additives, by the utilization of low molecular weight oligomers, and/or by physical fragmentation and subsequent loss of small fragments. Evidence for a (partial) biotic and/or abiotic oxidation of the amorphous polymer fraction and of surface-exposed hydrocarbon side chains is not sufficient to claim that PE is biodegradable. To the best of my knowledge, no report has been so far published in which substantial biodegradation and mineralization of PE or related (long chain length) hydrocarbon polymers to carbon dioxide has been convincingly demonstrated by the determination of the fate of carbon atoms in isotope-labeled polymers. It is disappointing that publications with a critical view on biodegradation of hydrocarbon polymers are not cited in most of these reports. The possibility should be considered that the rapidly expanding research field of hydrocarbon polymer biodegradation is chasing rainbows.

Adsorption and sensing properties of TiO2-modified MoSe2 monolayer for SF6 decomposition components based on the first-principles

As crucial components in power systems, SF6 gas-insulated equipment inevitably develops insulation defects and faults over long-term operation, leading to the decomposition of SF6 gas. Therefore, detecting SF6 decomposition components is essential for assessing the operational status of SF6 gas-insulated equipment and ensuring the safe and stable operation of power systems. Based on first principles, the modification mechanism of TiO2 on MoSe2 was explored, and optimal adsorption models for gases SO2, H2S, SOF2, and SO2F2 on this composite material were established. The adsorption characteristics of each system were analyzed through parameters such as adsorption energy, adsorption distance, charge transfer, electron density difference, and density of states, and the sensing properties of the systems were discussed using parameters including changes in frontier molecular orbitals, desorption time, and sensitivity. The results indicate that TiO2–MoSe2 has difficulty capturing SO2F2, but exhibits chemisorption for SO2, H2S, and SOF2 with adsorption energies of −1.25 eV, −0.99 eV, and −3.40 eV, respectively. Upon adsorption of SO2, TiO2–MoSe2 shows a significant change in electrical conductivity, a desorption time of 4.51 s (at 498 K), and a strong sensitivity response. However, the response to H2S is relatively weak, with lower sensitivity. Therefore, this material is suitable for detecting SO2 but not H2S. TiO2–MoSe2 demonstrates a significant response and excellent sensitivity to SOF2; however, the adsorption process lacks repeatability. Therefore, it can be used as a disposable gas sensor or solid adsorbent for SOF2 detection. The results of this study provide theoretical support for the application of MoSe2-based sensors in the monitoring and fault diagnosis of SF6 gas-insulated equipment.

Characterization of temperature regulating, high-temperature rheological and anti-aging properties of asphalt mastic modified with SSPCMs

The objective of this study is to explore the potential of utilizing self-developed SSPCMs as additives for heat storage temperature regulation in asphalt mastic modification. To achieve this aim, a variety of asphalt mastics with different F-A volume ratios and SSPCM contents were formulated, and their high-temperature rheological properties, modification mechanisms, thermoregulation capabilities, and aging resistance performances were analyzed. The results of temperature regulation test indicated that the maximum cooling amplitude of asphalt mastic could reach 11.3°C, showing a great promising application for cooling asphalt pavements. Additionally, the incorporation of SSPCMs generally resulted in positive impacts on both the high-temperature rheological properties and temperature sensitivity of the asphalt mastics. Moreover, the aging indices GAI, ICO and ISO of the modified asphalt mastic with 30 % SSPCMs decreased by 5.6 %, 18.3 % and 8.7 %, respectively, compared with control asphalt mastic, implying that the incorporation of SSPCMs can retard the aging process of asphalt and enhance the thermal oxidative aging resistance of the asphalt mastics. In summary, the current study demonstrates the feasibility, improved performance, and effective thermoregulation properties of SSPCMs in asphalt mastics.

Fenamates: Forgotten treasure for cancer treatment and prevention: Mechanisms of action, structural modification, and bright future.

Fenamates as classical nonsteroidal anti-inflammatory agents are widely used for relieving pain. Preclinical studies and epidemiological data highlight their chemo-preventive and chemotherapeutic potential for cancer. However, comprehensive reviews of fenamates in cancer are limited. To accelerate the repurposing of fenamates, this review summarizes the results of fenamates alone or in combination with existing chemotherapeutic agents. This paper also explores targets of fenamates in cancer therapy, including COX, AKR family, AR, gap junction, FTO, TEAD, DHODH, TAS2R14, ion channels, and DNA. Besides, this paper discusses other mechanisms, such as regulating Wnt/β-catenin, TGF-β, p38 MAPK, and NF-κB pathway, and the regulation of the expressions of Sp, EGR-1, NAG-1, ATF-3, ErbB2, AR, as well as the modulation of the tumor immune microenvironment. Furthermore, this paper outlined the structural modifications of fenamates, highlighting their potential as promising leads for anticancer drugs.

Research Progress on Saccharide Molecule Detection Based on Nanopores.

Saccharides, being one of the fundamental molecules of life, play essential roles in the physiological and pathological functions of cells. However, their intricate structures pose challenges for detection. Nanopore technology, with its high sensitivity and capability for single-molecule-level analysis, has revolutionized the identification and structural analysis of saccharide molecules. This review focuses on recent advancements in nanopore technology for carbohydrate detection, presenting an array of methods that leverage the molecular complexity of saccharides. Biological nanopore techniques utilize specific protein binding or pore modifications to trigger typical resistive pulses, enabling the high-sensitivity detection of monosaccharides and oligosaccharides. In solid-state nanopore sensing, boronic acid modification and pH gating mechanisms are employed for the specific recognition and quantitative analysis of polysaccharides. The integration of artificial intelligence algorithms can further enhance the accuracy and reliability of analyses. Serving as a crucial tool in carbohydrate detection, we foresee significant potential in the application of nanopore technology for the detection of carbohydrate molecules in disease diagnosis, drug screening, and biosensing, fostering innovative progress in related research domains.

Investigation of surface finish and fatigue life of laser Powder Bed fused Ti-6Al-4V

In this fact-pacing world of technological advancement, the rise of additive manufacturing (AM) has found immense applications of metallic alloys such as Ti6Al4V to manufacture key components in automotive, aerospace and healthcare sectors. However, fatigue characteristics of such additively manufactured metal alloys is noticeably weak, and as such requires post-processing. In this study, AM Ti6Al4V manufactured by Laser Powder Bed Fusion (L-PBF) was treated by Hydrodynamic Cavitation Abrasive Finishing (HCAF) and Laser Cavitation Peening (LCP) to observe the improvement in surface roughness as well as fatigue performance. Our investigations revealed a substantial improvement in surface quality post-HCAF processing. Notably, the surface roughness of AM Ti6Al4V specimens showed a significant reduction of about 91.2 %, post surface treatment. However, Laser Cavitation Peening (LCP) treated sample demonstrates a significantly enhanced fatigue strength of 393 ± 22 MPa at 107, which was 1.75 times better than that of as-built one, i.e., 224 ± 16 MPa. This difference can be attributed to the distinct surface modification mechanisms of each technique. LCP, utilizing laser-induced shock waves, likely induces large compressive residual stresses, leading to better fatigue resistance.

Mechanical properties and strain rate effect of graphene oxide grafted carbon fiber modified concrete under dynamic impact load

In order to improve the shortcomings of carbon fiber, such as smooth surface, poor bonding with concrete matrix and easy to pull out under dynamic impact load, graphene oxide is grafted onto the surface of carbon fiber by chemical bonds to prepare graphene oxide grafted carbon fiber (CF-GO). The dynamic impact test on CF-GO modified concrete (CF-GOMC) is carried out by the split hopkinson pressure bar (SHPB) test system, and the mechanical properties and strain rate effect of CF-GOMC under dynamic impact loads are studied. The modification mechanism of CF-GO is analyzed by microscopic tests. The results show that compared with carbon fiber, the surface roughness and surface area of CF-GO increase and CF-GO is more suitable for bonding with concrete matrix. CF-GO can improve the mechanical properties of concrete under dynamic impact load and enhance the strain rate effect of dynamic mechanical properties indicators by preventing crack, bridging, filling and promoting hydration. With the increase of CF-GO content, the dynamic mechanical properties and strain rate sensitivity of CF-GOMC increase first and then decrease. The optimal content of CF-GO is 0.3 %. The improvement effect of CF-GO on the dynamic mechanical properties and strain rate effect of concrete is better than that of carbon fiber. The graphene oxide on the surface of CF-GO makes the interface of CF-GO/concrete matrix compact by improving the mechanical bite force of fiber/concrete matrix and promoting the formation of hydration products on the surface of fiber. The addition of CF-GO does not change the composition of cement hydration products, but promotes the crystal growth of hydration products and optimizes the pore structure of concrete.

Mechanism of chloride modification ambipolar polymer electrode materials for high-performance energy storage device

Ambipolar conducting polymer electrode materials exhibit a wide voltage window, contributing to fast development of high-energy–density storage devices. The doping levels of p-doped and n-doped in ambipolar conducting polymers determine the electrochemical performance of energy storage devices. However, n-doped materials always face low doping efficiency, caused by difficulty in electron injection and ion migration, thereby affecting specific capacitance and stability. In this study, two ambipolar conductive polymers were developed, in which the n-doped imine group was chlorinated to improve electron injection, and the mechanism of ion migration process was studied by changing the chlorination of different positions relative to the carbonyl group. The influence of chloride modification on n-doped electrochemical energy storage was thoroughly investigated. The results show that chloride modification can lower the lowest unoccupied molecular orbital (LUMO) energy level of the monomer and make electron injection easier. Interestingly, the chloride modification position not only affects the oxidation–reduction of the carbonyl, but also the binding of the carbonyl with lithium ions. If the chloride modification position is too close to the carbonyl, lithium-ion migration in ambipolar conducting polymer will be restricted, although electron injection is beneficial. Based on this design, the developed PEPCl conducting polymer exhibits excellent electrochemical performance, and the mechanism of chlorination modification of ambipolar polymer electrode materials is described.

The role of RNA modifications in hepatocellular carcinoma: functional mechanism and potential applications.

Hepatocellular carcinoma (HCC) is a highly aggressive cancer with a poor prognosis. The molecular mechanisms underlying its development remain unclear. Recent studies have highlighted the crucial role of RNA modifications in HCC progression, which indicates their potential as therapeutic targets and biomarkers for managing HCC. In this review, we discuss the functional role and molecular mechanisms of RNA modifications in HCC through a review and summary of relevant literature, to explore the potential therapeutic agents and biomarkers for diagnostic and prognostic of HCC. This review indicates that specific RNA modification pathways, such as N6-methyladenosine, 5-methylcytosine, N7-methylguanosine, and N1-methyladenosine, are erroneously regulated and are involved in the proliferation, autophagy, innate immunity, invasion, metastasis, immune cell infiltration, and drug resistance of HCC. These findings provide a new perspective for understanding the molecular mechanisms of HCC, as well as potential targets for the diagnosis and treatment of HCC by targeting specific RNA-modifying enzymes or recognition proteins. More than ten RNA-modifying regulators showed the potential for use for the diagnosis, prognosis and treatment decision utility biomarkers of HCC. Their application value for HCC biomarkers necessitates extensive multi-center sample validation in the future. A growing number of RNA modifier inhibitors are being developed, but the lack of preclinical experiments and clinical studies targeting RNA modification in HCC poses a significant obstacle, and further research is needed to evaluate their application value in HCC treatment. In conclusion, this review provides an in-depth understanding of the complex interplay between RNA modifications and HCC while emphasizing the promising potential of RNA modifications as therapeutic targets and biomarkers for managing HCC.

A novel lncRNA GM47544 modulates triglyceride metabolism by inducing ubiquitination-dependent protein degradation of APOC3

ObjectiveEmerging evidence highlights the pivotal roles of long non-coding RNAs (lncRNAs) in lipid metabolism. Apoprotein C3 (ApoC3) is a well-established therapeutic target for hypertriglyceridemia and exhibits a strong association with cardiovascular disease. However, the exact mechanisms via which the lncRNAs control ApoC3 expression remain unclear. MethodsWe identified a novel long noncoding RNA (lncRNA), GM47544, within the ApoA1/C3/A4/A5 gene cluster. Subsequently, the effect of GM47544 on intracellular triglyceride metabolism was analyzed. The diet-induced mouse models of hyperlipidemia and atherosclerosis were established to explore the effect of GM47544 on dyslipidemia and plaque formation in vivo. The molecular mechanism was explored through RNA sequencing, immunoprecipitation, RNA pull-down assay, and RNA immunoprecipitation. ResultsGM47544 was overexpressed under high-fat stimulation. GM47544 effectively improved hepatic steatosis, reduced blood lipid levels, and alleviated atherosclerosis in vitro and in vivo. Mechanistically, GM47544 directly bound to ApoC3 and facilitated the ubiquitination at lysine 79 in ApoC3, thereby facilitating ApoC3 degradation via the ubiquitin-proteasome pathway. Moreover, we identified AP006216.5 as the human GM47544 transcript, which fulfills a comparable function in human hepatocytes. ConclusionsThe identification of GM47544 as a lncRNA modulator of ApoC3 reveals a novel mechanism of post-translational modification, with significant clinical implications for the treatment of hypertriglyceridemia and atherosclerosis.

Relaxation and entropy generation in dewetting thin glassy polymer films trapped far from equilibrium

AbstractPolymers when confined to a dimension comparable to the length scale of polymer chain coils such as thin films, often lead to molecular relaxation processes distinct from their bulk counterpart. Often observed as thermal and mechanical responses such relaxation has been frequently associated with the squeezing of polymer chains having conformations trapped far from thermodynamic equilibrium and subsequently generating processing‐induced molecular recoiling stress. Relaxation in polymer films can be modified by tuning the molecular recoiling stress, which is directly influenced by the preparation conditions of the polymer thin films. Hence a comprehensive understanding of the genesis and relaxation of molecular recoiling stress becomes necessary. Here, we provide insights into the nonequilibrium nature observed in polymer thin films, focusing majorly on the investigations into the molecular recoiling stress using the dewetting technique. The impact of various factors like temperature of dewetting, thickness of films, molecular weight of polymers, and physical aging affecting the relaxation of molecular recoiling stress is discussed. In addition, discussions on the possible mechanisms of relaxation and modification of molecular recoiling stress by varying the spin‐coating speed and addition of plasticizers are also provided. An alternate approach which gives a new perspective into the relaxation of molecular recoiling stress considering the entropy generated during the dewetting of polymer films is also included. The present work is expected to give the reader a comprehensive understanding of the characteristics of molecular recoiling stress relaxation occurring in polymer thin films.

In-situ deposition modification of hydrophilic polytetrafluoroethylene (PTFE) membranes using surfactants as anchoring points for long-term stability

Polytetrafluoroethylene (PTFE) membranes are renowned for their excellent thermal stability, resistance to strong acids and alkalis, and superior mechanical stability. However, its inherent strong hydrophobicity significantly limits its use in water treatment. In this study, a SiO2 coating was successfully deposited in-situ on the surface of PTFE membranes using a hydrolyzed solution of tetraethyl orthosilicate (TEOS) compounded with the commercial fluorinated polyoxyethylene ether surfactant FS-31 (C6F13CH2CH2O(CH2CH2O)nH). The modification mechanism involves using the low surface energy nonionic fluorocarbon surfactant FS-31, which interacts hydrophobically with the PTFE substrate, serving as an “anchor” point around which the TEOS hydrolyzes and condenses around the fibers and nodes of the PTFE membrane, forming a complete and stable chemical network structure. The results show that the initial water contact angle of the modified membrane dropped to 36.5°, and the water flux reached 537.9 L m−2 h−1. After being continuously immersed in strong acid (5 wt% HCl), strong alkali (4 wt% NaOH), and oxidizing agent (5 wt% NaClO) for one week, the membrane maintained excellent hydrophilicity and stability. This study proposes a mild and efficient modification method to prepare durable hydrophilic PTFE membranes, portending great potential for applications in harsh environments.

Metal-Organic Frameworks (MOFs): Classification, Synthesis, Modification, and Biomedical Applications.

Metal-organic frameworks (MOFs) are a new variety of solid crystalline porous functional materials. As an extension of inorganic porous materials, it has made important progress in preparation and application. MOFs are widely used in various fields such as gas adsorption storage, drug delivery, sensing, and biological imaging due to their high specific surface area, porosity, adjustable pore size, abundant active sites, and functional modification by introducing groups. In this paper, the types of MOFs are classified, and the synthesis methods and functional modification mechanisms of MOFs materials are summarized. Finally, the application prospects and challenges of metal-organic framework materials in the biomedical field are discussed, hoping to promote their application in multidisciplinary fields.

Sirt1 m6A modification-evoked Leydig cell senescence promotes Cd-induced testosterone decline

Diminished testosterone levels have been documented as a key factor in numerous male health disorders. Both human and animal studies have consistently demonstrated that cadmium (Cd), a pervasive environmental heavy metal, results in decreased testosterone levels. However, the exact mechanism through which Cd interferes with testosterone synthesis remains incompletely elucidated. This research sought to examine the impact of cellular senescence on Cd-suppressed testosterone synthesis. We also investigated the related m6A modification mechanism. The results demonstrated that Cd (100 mg/L) led to a decrease in testosterone levels, along with downregulated expression of testosterone synthase in C57BL/6 N male mice. Furthermore, Cd significantly increased β-galactosidase staining intensity, senescence-related proteins, and senescence-related secretory phenotypes in mouse testicular Leydig cells. Subsequent investigations revealed that Cd decreased the mRNA and protein levels of NAD-dependent deacetylase Sirtuin-1 (SIRT1) in Leydig cells. Mechanistically, mice treated with resveratrol (50 mg/kg), a specific SIRT1 activator, mitigated Leydig cell senescence and reversed Cd-reduced testosterone levels in mouse testes. These effects were also restored by SIRT1 overexpression in Leydig cells. Additionally, we found that Cd increased the level of methyltransferase enzyme METTL3 and Sirt1 m6A modification in Leydig cells. Mettl3 siRNA effectively restored Cd-enhanced Sirt1 m6A level and reversed Cd-downregulated Sirt1 mRNA expression in Leydig cells. Overall, our findings suggest that Cd exposure inhibits testosterone synthesis via Sirt1 m6A modification-mediated senescence in mouse testes. These results offer an experimental basis for investigating the causes and potential treatments of hypotestosteronemia induced by environmental factors.

Effects of ultrasonic waves of different powers on the physicochemical properties, functional characteristics, and ultrastructure of bovine liver peptides

In recent years, ultrasound has emerged as a widely used technology for modifying proteins/peptides. In this study, we focused on the intrinsic mechanism of ultrasound-induced modification of bovine liver peptides, which were treated with ultrasound power of 0, 100, 200, 300, 400, and 500 W, and their physicochemical and functional properties, as well as ultrastructures, were investigated. The results show that ultrasound mainly affects hydrogen bonding and hydrophobic interactions to change the conformation of proteins and unfolds proteins through a cavitation effect, leading to an increase in biological activity. Fourier infrared spectroscopy showed that ultrasound inhibited the formation of hydrogen bonds and reduced intermolecular cross-linking. Molecular weight distribution showed that the antioxidant components of bovine liver polypeptides were mainly concentrated in fractions of 500–1,000 Da. Maximum values of ABTS (82.66 %), DPPH (76.02 %), chelated iron (62.18 %), and reducing power (1.2447) were obtained by treating bovine liver polypeptides with 500 W ultrasound. Combined with the scanning electron microscopy results, with the intervention of ultrasound, the impact force generated by ultrasonication may lead to the loosening of the protein structure, which further promotes the release of antioxidant peptides, and these findings provide new insights into the application of ultrasound in the release of antioxidant peptides from bovine liver.

Modified Titanium Implants Satisfy the Demands of Diabetic Osseointegration via Sequential Regulation of Macrophages and Mesenchymal Stem Cells.

The application of titanium (Ti) implants for patients with diabetes mellitus (DM) is still facing a significant challenge due to obstacles such as hyperglycemia, reactive oxygen species (ROS), and chronic inflammation, which hinders osseointegration. To address this issue, a Ti implant with dual functions of regulating polarization of macrophages and facilitating osseointergration is developed via hydrothermal reaction and hydrogel coating. The reactive oxygen species (ROS) and glucose (Glu) responsive hydrogel coating can locally deliver adenosine (ADO) in the early stage of implantation. The controlled release of ADO regulated the phenotype of macrophages, restored oxidative balance, and enhanced mitochondrial function during the early stages of implantation. Subsequently, strontium (Sr) ions will be released to promote osteogenic differentiation and proliferation of mesenchymal stem cells (MSCs), as the hydrogel coating degraded. It eventually leads to bone reconstruction during the late stages, aligning with the biological cascade of bone healing. The modified Ti implants showed effective osteogenesis for bone defects in DM patients, shedding light on the design and biological mechanisms of surface modification. This research offers promising potential for improving the treatment of bone-related complications in diabetic patients.

Exploring the effects of three-finger toxins from Naja ashei venom on neuronal and immunological cancer cell membranes

Three-finger proteins are the most abundant toxins in the venom of Naja ashei, a snake species from the Elapidae family. This research aimed to describe the effects of varying charges of these proteins, isolated from Naja ashei venom using SEC and IEX chromatography. The study examined how differently charged three-finger toxin fractions interact with and affect neuroblastoma (SK-N-SH) and promyeloblast (HL-60) cells, as well as model Langmuir membranes and liposomes designed to mimic cellular lipid composition. Findings revealed that protein surface charges significantly impact cell survival (MTT assay), membrane damage (lactate dehydrogenase release, malondialdehyde formation), and the structural and electrochemical properties of model membranes (Langmuir membranes and zeta potential for liposomes and cancer cell lines). Results indicated that SK-N-SH cells, characterized by a higher negative charge on their cell membranes, interacted more effectively with positively charged toxins than HL-60 cells. However, the mechanism of these electrostatic interactions is complex. The research demonstrated that electrostatic and mechanical membrane modifications induced by venom proteins can significantly affect cell metabolism. Additionally, the total charge of the membrane, influenced by polar lipid components and phospholipid saturation, plays a decisive role in toxin interaction.