Minced Meat Research Articles

Development of a novel colorimetric pH-indicator film based on CMC/flaxseed gum/betacyanin from beetroot peels: A powerful tool to monitor the beef meat freshness

A novel indicator film based on carboxymethyl-cellulose (CMC)/flaxseed gum (FG) was developed by introducing betacyanin (BC) from beetroot peel extract. Remarkably, CMC/FG/BC film exhibited a dynamic color transition from pink to yellow in response to pH changes from 1 to 12. Beyond its pH sensitivity, the newly developed film demonstrated exceptional sensitivity to ammonia solution concentrations, detecting variation from 0.125 to 0.5 mg/mL with a significant ΔE (P < 0.05) change when a concentration of 0.5 mg/mL was added. Moreover, the inclusion of BC not only heightened the film's colorimetric capabilities but also enhanced the antioxidant, physical (moisture content, swelling index, thickness and biodegradability), and mechanical (elongation at break) properties. On the other hand, the CMC/FG/BC film's ability to provide real-time monitoring of minced beef meat quality was examined during 14 days of storage at 4 °C. The CMC/FG/BC film displayed a distinctive color change (from light pink to purple) corresponding to changes in meat samples' total volatile basic nitrogen (TVB-N) and microbial count (aerobic plate, psychrotrophic total and Enterobacteriaceae counts) during the storage period. The CMC/FG/BC film could be considered as a novel colorimetric pH indicator to monitor meat and meat derivatives' freshness. Its multifaceted properties make it valuable as an intelligent packaging used in food quality control, offering real-time insights into both pH changes and the freshness of stored meat products.

Using Essential Oils to Reduce Yersinia enterocolitica in Minced Meat and in Biofilms.

Yersiniosis, one of the leading foodborne infections in the European Union, is caused by Yersinia enterocolitica. In this study, the antibacterial and antibiofilm effects of cinnamon (Cinnamomum zeylanicum Nees), clove (Syzygium aromaticum L.), oregano (Origanum vulgare L.), rosemary (Rosmarinus officinalis L.), thyme (Thymus vulgaris L.), and winter savory (Satureja montana L.) essential oils were investigated against Y. enterocolitica strains belonging to the bioserotype 4/O:3. Cinnamon essential oil showed the highest antibacterial activity, with an MIC value 0.09 µL/mL, followed by oregano and thyme essential oils, with MIC values from 0.09 to 0.18 µL/mL, and from 0.18 to 0.23 µL/mL, respectively. Thyme essential oil at 0.23 µL/g (MIC) and at 0.46 µL/g (2MIC) significantly (p < 0.05) reduced the number of Y. enterocolitica by 0.38 log CFU/g and 0.64 log CFU/g, respectively, in minced pork meat during storage at 4 °C for 4 days. The Y. enterocolitica strains formed biofilms at 15 °C and 37 °C in tryptic soy broth and Luria-Bertani broth, while no biofilms were obtained at 5 °C, and in meat broth nutrient media. Applying the minimum bactericidal concentrations of cinnamon, clove, oregano, rosemary, thyme, and winter savory essential oils on preformed biofilms led to significant reductions being observed in the range from 45.34% to 78.89%. A scanning electron microscopy assay showed the devastating impact of oregano and thyme essential oils on the morphology of Y. enterocolitica bacterial cells. In conclusion, the results of this study show that essential oils possess high anti-Yersinia and antibiofilm effects.

Design and Optimization of a yst-PCR to Detect Yersinia enterocolitica in Meat Food.

In this study, a polymerase chain reaction (PCR) directed to the yst chromosomal gene (yst-PCR) was used as a rapid, sensitive, and specific method to detect Yersinia enterocolitica strains belonging to different biotypes in foods; a competitive Internal Amplification Control (cIAC) is also developed. The cIAC had a molecular weight of 417 bp and was detected until a concentration of 0.85 ng/μL. No other strains of other Yersinia species, nor Enterobacteriales order were detected by this PCR. In pure culture, the detection limit (DL) of the yst-PCR was lower for ystA+ strain (10 colony-forming unit [CFU]/mL) than for ystB+ strain (1 × 102 CFU/mL); which was the concentration detected in Y. enterocolitica inoculated minced meat. The proposed protocol included an enrichment step in peptone sorbitol bile (PSB) broth at 25°C for 24 h followed by isolation on Mac Conkey agar and chromogenic medium. An aliquot of the PSB broth homogenate and a loopful from the confluent zone of solid media were collected to perform DNA extraction for yst-PCR, and typical colonies were characterized by biochemical assays. Among 30 non-contaminated food samples, 4 samples were yst-positive and no Y. enterocolitica isolates were obtained. It is suggested that this yst-PCR could be used in the investigation of Y. enterocolitica in foods.

ВОЗМОЖНОСТЬ ИСПОЛЬЗОВАНИЯ ПОРТУЛАКА ОГОРОДНОГО (PORTULACA OLERACEA L.) ДЛЯ ОБОГАЩЕНИЯ МЯСНЫХ РУБЛЕНЫХ ИЗДЕЛИЙ

The purpose of the study is to identify the possibility of using the aerial part of purslane in cutlet recipes. Objectives: to determine the dosage of purslane in ground turkey meat, ensuring the best quality indicators of the product; to conduct a tasting assessment of the product; to develop a schematic diagram for the production of semi-finished meat and vegetable products. The components for the production of chopped semi-finished meat products complied with the regulatory documentation: turkey meat – in accordance with GOST R 55365-2012 “Mince meat. Technical conditions" and GOST 32951-2014 "Semi-finished meat and meat-containing products. General technical conditions"; chicken eggs – according to GOST 31654-2012 “Chicken eggs for food. Technical conditions"; onions – according to GOST 34306-2017 “Fresh onions. Technical conditions"; black pepper – according to GOST 29050-91 “Spices. Black and white pepper. Technical conditions"; table salt – according to GOST R 51574-2018; vegetable oil – according to GOST 1129-2013; breadcrumbs – according to GOST 28402-89. Organoleptic and tasting assessment of finished products was carried out in accordance with GOST 31986-2012 (GOST 7269-2015 and GOST 9959-2015) on a 5-point scale. A recipe was developed for turkey meat cutlets with the addition of a plant component – purslane. Purslane was collected in the vicinity of Krasnoyarsk and was added crushed to minced meat in dosages from 3 to 9 % in increments of 3 instead of raw meat. The study of the organoleptic characteristics of the finished minced meat product showed that the rational dosage introduced into the meat cutlet mass of the vegetable ingredient is 6 % of the mass of the minced meat. The test sample with the introduction of 6 % plant component received the highest rating from tasters. Stages for the production of cutlets have been developed with the addition of chopped aerial parts of purslane to the recipe. The use of purslane in the recipe for chopped semi-finished meat products will help expand the range of this type of product, improve the consumer properties of meat products, increase the content of essential components in the composition, and reduce costs.

Modelling the centrifugal mixing process of minced meat to optimise the production of chopped meat semi-finished products

One of the most important problems in ensuring the quality of mincemeat preparation in the production of sausages is the effective structuring of components and mixing of their ingredients. To solve this problem, researchers added a multifunctional admixture based on whey protein in the process of centrifugal mixing of the components, which determined the composition of the factor space of the investigated process. Based on the results of the research, the effective content of whey protein, sodium alginate, and soy fiber in the developed recipe was proven, which showed high characteristics in terms of fat-retaining and moisture-retaining ability, digestibility, pH level – activity, and other parameters. The developed formulation made it possible to improve the general indicator of the balance of amino acids in the product and increase the functional-technological and quality parameters of the developed products. The physical and mechanical characteristics of the obtained meat product were evaluated based on the results of physical and mathematical modelling. Modelling was carried out using Federman-on-Buckingham's second similarity theory and the "dimension theory" method, which allows the processing of the obtained experimental data in the form of a criterion equation, which was compiled using Froude, Euler, and Sherwood criteria. The purpose of this study was to obtain dependencies between such process factors as product density, the coefficient of dynamic viscosity of the technological medium, the ultimate shear stress, the change in the concentration of the main impurities of lactic acid in the raw material, the value of the diffusion coefficient and the coefficient of mass transfer in the loading mass, the weight of one load of products, the angular frequency of rotation of the screws of the minced meat mixer, the radius of the rotating working bodies, the characteristic size of the products after grinding. Using the complex criterion equation and the developed program, we find a recommended set of operating mode parameters for preparing minced meat under the conditions of centrifugal influence on the mixing process and the action of the specified factors.

ПЕРСПЕКТИВИ ВИРОБНИЦТВА КВАСОЛІ В КОНТЕКСТІ НІШЕВОЇ ДИВЕРСИФІКАЦІЇ СІЛЬСЬКОГО ГОСПОДАРСТВА ТА НАРОЩУВАННЯ ЙОГО ЕКСПОРТНОГО ПОТЕНЦІАЛУ

The global trends in the production and trade of beans were analyzed, the level and dynamics of export-import operations of Ukraine in this market segment were investigated, the prospects of crop production were substantiated in the context of niche diversification of the domestic agrarian sphere and the expansion of its export potential. It is noted that during the last decade, the volume of world production of beans has remained relatively stable within 29 million tons. The leading countries are India and Brazil, the combined share of which in the world crop harvest in 2022 was 36.2%. A third of the world's bean exports go to Myanmar – the main exporter of legumes to India. The main importers of beans are India and China, these countries supply 32.5% of the world's import of the crop.In the overall structure of foreign trade, the share of Ukrainian beans in 2022 was 1.2%. During 2018-2022, the value of culture exports from Ukraine was characterized by an upward trend. Almost all export (99.9%) and import products (95.4%) are white bean varieties The main buyers of Ukrainian beans were Italy, Pakistan and Poland. In 2022, 53.4% of exports were sent to these countries. On the foreign market, Ukraine mainly buys elite bean seeds. In 2022, 85.6% of its total imports were supplied from Poland. It is noted that in modern conditions, beans are a promising niche crop for the domestic agriculturist, since logistics is less affected by it than grain crops, the demand in foreign markets is high, in addition to economic, the introduction of beans in crop rotation also provides an ecological effect. The export potential of beans is determined by the growth of global demand for processed legume products (chips, hummus, vegetable minced meat, nuggets, burger patties, etc.).

Effect of deacetylated konjac glucomannan on the 3D printing properties of minced pork.

The influences of deacetylated konjac glucomannan (DKGM) at different condition levels (0.0%, 0.5%, 1.0%, 1.5%, 2.0%) on the 3D printing feasibility, printing properties, and the final gel characteristics of minced pork were investigated. As the DKGM content increased, the printing accuracy and stability initially increased and then declined, and the printing stability and accuracy increased to their highest levels (98.16% and 98.85%) with a 1.5% addition of DKGM. Furthermore, the addition of DKGM significantly enhanced the texture of 3D-printed meat after heat treatments. When the DKGM content reached 1.5%, the hardness and springiness were 1.19 and 1.06 times higher than those of the control group. The results of low-field nuclear magnetic resonance and Raman spectra revealed that DKGM enhanced the amount of bound water in 3D-printed meat and encouraged changes in protein structure. After the addition of DKGM at 1.5%, the contents of bound water and β-sheets were 7.67% and 12.89% higher than those of the control group, respectively, facilitating the development of a better gel network of minced meat during heating. The results indicate that a concentration of 1.5% DKGM is the ideal setting for obtaining the desired rheological properties and textural characteristics (printability) of 3D-printed minced meat products compared to other samples. In addition, the results showed that the addition of DKGM at 1.5% promotes the transition from α-helix to β-folding of proteins during heating, which facilitates the formation of gels. The results of the study contribute to the application potential of minced meat in the field of 3D food printing. © 2024 Society of Chemical Industry.

Occurrence and Characteristics of Toxigenic Staphylococcus aureus in Retail Foods in Iran.

Staphylococcus aureus causes various toxigenic and invasive diseases in humans worldwide. This study examined the prevalence, virulence genes, and antibiotic resistance of S. aureus isolates collected from 894 retail food samples in Ardabil, Iran. Staphylococcal cassette chromosome mec (SCCmec), spa, and multilocus sequence typing methods were employed to further investigate the molecular characteristics of methicillin-resistant S. aureus (MRSA) isolates. The results revealed that 11.18% (n = 100) of food samples exhibited contamination with S. aureus (10.50% methicillin-sensitive S. aureus [MSSA] and 0.67% MRSA). Notably, raw minced meat (29.41%), Faloodeh (25%), and Olivier salad (21.42%) emerged as the most frequently contaminated food items. Among the 100 isolates of S. aureus, 94% were characterized as MSSA, with the remaining 6% identified as MRSA. The highest resistance was observed for penicillin (12%). MRSA isolates exhibited significantly higher resistance rates. Seventy-nine percent of the isolates were positive for sea, 14% for seb, 8% for a sec, and 0% for sed enterotoxin-encoding genes. Sixteen percent of isolates harbored two or more staphylococcal enterotoxin genes, simultaneously. Moreover, 97%, 94%, 24%, and 22% of isolates were positive for hla, hld, tst, and pvl virulence-encoding genes, respectively. No isolate was positive for the exfoliative toxins encoding eta and etb genes. MRSA isolates belonged to CC8 (n = 4) and CC22 (n = 2). Isolates in CC8 belonged to lineage ST239-MRSA-III and spa type t030; the isolates in CC22 belonged to ST22-MRSA-IV and spa types t310 and t223. In conclusion, a relatively high proportion of our retail food samples were contaminated with S. aureus. The high incidence of isolates with toxigenic genes raises serious health concerns. Furthermore, the presence of MRSA lineages linked to humans suggests that retail foods may be contaminated with human origin.

Meat and meat products as potential sources of emerging MDR Bacillus cereus: groEL gene sequencing, toxigenic and antimicrobial resistance

BackgroundBacillus cereus is implicated in severe foodborne infection in humans. This study intended to assess the occurrence, groEL gene sequencing, biofilm production, and resistance profiles of emerged multidrug resistant (MDR) B. cereus in meat and meat product samples. Moreover, this work highlights the virulence and toxigenic genes (hblABCD complex, nheABC complex, cytK, ces, and pc-plc) and antimicrobial resistance genes (bla1, tetA, bla2, tetB, and ermA).MethodsConsequently, 200 samples (sausage, minced meat, luncheon, beef meat, and liver; n = 40 for each) were indiscriminately collected from commercial supermarkets in Port Said Province, Egypt, from March to May 2021. Subsequently, food samples were bacteriologically examined. The obtained isolates were tested for groEL gene sequence analysis, antibiotic susceptibility, biofilm production, and PCR screening of toxigenic and resistance genes.ResultsThe overall prevalence of B. cereus among the inspected food samples was 21%, where the highest predominance was detected in minced meat (42.5%), followed by beef meat (30%). The phylogenetic analysis of the groEL gene exposed that the examined B. cereus strain disclosed a notable genetic identity with other strains from the USA and China. Moreover, the obtained B. cereus strains revealed β-hemolytic activity, and 88.1% of the recovered strains tested positive for biofilm production. PCR evidenced that the obtained B. cereus strains usually inherited the nhe complex genes (nheA and nheC: 100%, and nheB: 83.3%), followed by cytK (76.2%), hbl complex (hblC and hblD: 59.5%, hblB: 16.6%, and hblA: 11.9%), ces (54.7%), and pc-plc (30.9%) virulence genes. Likewise, 42.9% of the examined B. cereus strains were MDR to six antimicrobial classes and encoded bla1, bla2, ermA, and tetA genes.ConclusionIn summary, this study highlights the presence of MDR B. cereus in meat and meat products, posing a significant public health risk. The contamination by B. cereus is common in minced meat and beef meat. The molecular assay is a reliable fundamental tool for screening emerging MDR B. cereus strains in meat and meat products.

Minced Beef Meat Paste Characteristics: Gel Properties, Water Distribution, and Microstructures Regulated by Medium Molecular Mass of γ-Poly-Glutamic Acid.

The influences of various m-γ-PGA (0.08-0.20%, w/w) concentrations on the properties of minced beef meat paste in terms of rheological properties, texture, moisture distribution, and microstructures were evaluated. The results indicated that m-γ-PGA enhanced the water-holding capacity, gel strength, texture, and whiteness of the minced beef meat paste. Based on the microstructural results, m-γ-PGA helped form a more organized and compact gel, thereby limiting the migration of water through the gel matrix. In contrast to the control group, the water-holding property, gel strength, and whiteness of minced meat paste gels with m-γ-PGA content of 0.12% increased from 75.89%, 584.51 g·cm, and 61.83 to 79.91%, 780.87 g·cm, and 62.54, respectively (p < 0.05), exhibiting the highest water-holding property and gel strength. Thus, m-γ-PGA exhibits great potential for minced meat paste products as a healthy gel water retainer and enhancer in low-fat meat products.

Antimicrobial Activity of Some Essential Oils as Natural Preservatives in Minced Meat

Antimicrobial Activity of Some Essential Oils as Natural Preservatives in Minced Meat

EVALUATION EFFECT OF ANTIMICROBIAL NANOCELLULOSE FILM COMBINED WITH LACTOBACILLUS RHAMNOSUS POSTBIOTICS IN ACTIVE PACKAGING OF MINCED MEAT

Minced meat (MM) has a high surface-to-volume ratio, which makes it prone to the growth of pathogenic and spoilage bacteria due to increased exposure to oxygen and high nutrient levels. In this study, postbiotics were used as a natural preservative to extend the shelf life of MM. Lyophilized postbiotics from Lactobacillus rhamnosus were incorporated into bacterial nanocellulose (BNNC) to create an antimicrobial film for packaging MM. The effectiveness of the postbiotic-embedded BNNC film against Staphylococcus aureus was tested using the disk diffusion method and the optimal postbiotic concentrations were determined using the microdilution broth method. Structural changes of the BNNC film after immersion in postbiotics were examined using scanning electron microscopy, and the attachment and chemical nature of the functional groups in the sample were confirmed using FTIR. The antimicrobial efficacy of the postbiotic-embedded BNNC film against S. aureus in MM was also tested. The results showed that BNNC with 10% (P10-BNNC) and 4% (P4-BNNC) postbiotic concentrations were the optimal films, as confirmed by SEM and FTIR. The P-BNNC films significantly reduced the number of S. aureus during storage of MM for 9 days at 4°C. In conclusion, BNNC can serve as a suitable carrier for creating antimicrobial films using postbiotics derived from LAB for food packaging applications.

Tailoring an egg white protein double network emulsion gel as a novel fat substitute for improving freeze-thaw stability of minced meat gel

This work aimed to investigate the effect of double network emulsion gel (DNEG) as a substitute for pork back fat (PBF) on the freeze-thaw stability of minced meat gel (MMG). We used slow acidification with glucono-δ-lactone (GDL) to induce egg white protein (EWP) gelation, while CaCO3 was affected by acidification to release Ca2+ to cross-link sodium alginate (SA), resulting in the gradual formation of DNEG networks. The MMG prepared using DNEG substitute for PBF had fine hardness and color after 5 freeze-thaw cycles (FTCs). This was due to the excellent plasticity of DNEG, which made it more capable of filling the voids of minced meat products, thus reducing the space for ice crystal growth. As a result, MMG prepared with DNEG could pass through the maximum ice crystal formation zone faster and avoid damage due to the formation of larger ice crystals. Notably, DNEG provided a barrier for the migration of immobilized water to bulk water in the sample. After several freeze-thaw treatments, the water holding capacity of samples prepared with DNEG decreased significantly slower, which slowed down the oxidation and decomposition of meat proteins. In summary, this study revealed the role of fat substitute DNEG as a favorable filling material for improving the freeze-thaw stability of minced meat products, and provided a technical reference for the development of healthy and storable meat products.

Wild fennel seeds from Valle Agricola (Southern Italy): Biochemical and antioxidant traits in minced pork meat

Here, we report the nutritional (crude proteins, total and free amino acids, fatty acids, minerals) and biochemical (protease inhibitors and antioxidant enzymes) characterization of wild fennel seeds from ‘Valle Agricola’ (Caserta, Italy), typical local products used as a food flavouring and preservative. The comparative analysis with commercial fennel seeds revealed the higher antioxidant activity of wild fennel seeds and the considerable ability of the latter in food preservation. In light of this, both wild and commercial fennel seeds were added to minced pork meat to evaluate the effect of flavouring treatment at 0, 1, 3 and 7 days. The analysis revealed that free amino acids, fatty acids and glycosidically bound volatile compounds responsible for meat taste and aroma were mainly released following wild fennel seeds addition.Overall, these data encourage the local consumption of traditional spices, usually added to traditional dry-cured meat and open new opportunities for the niche market.

Occurrence, antimicrobial resistance, and molecular characterization of Salmonella enterica from chicken products and human in Wasit Governorate of Iraq

Background: Salmonella infections are considered the most common foodborne pathogens responsible for zoonotic infections and food poisoning in humans and animal species such as birds. Antimicrobial resistance is considered a global anxiety, because it cause human public health repercussions, as well as lead to increase in animal morbidity and death. Aim: The aims of this study are the isolation and identification of Salmonella enterica, as well as to investigate the antimicrobial susceptibility test and the molecular characteristics using PCR and sequences for isolates from chicken products (eggs, livers and minced meat) and human in the Wasit Governorate of Iraq. Methods: A total of 300 samples (150 chicken product samples including eggs, livers, and minced meat, and 150 human fecal samples) were collected from the Wasit governorate of Iraq from January to December 2022. The bacterial isolation was done according to recommendations of ISO 6579 standard and the Global Foodborne Infections Network laboratory protocol WHO 2010. Serotyping test and Antimicrobial susceptibility test (AST) were done by using 19 antibiotic agents according to the recommendations of CLSI, 2022 by using disc diffusion susceptibility test and Vitik 2 test. Finally, the suspected isolates were confirmed using the conventional polymerase chain reaction (PCR) method and sequencing for a unique rRNA gene. Results: The results showed that the isolation percentage of S. enterica in chicken products was 8.66% (12% eggs, 6% livers and 8% minced meat), while in humans it was 4.6%. Also, showed 100% of S. Typhi in human. While, in chicken eggs S. Typhi, S. Typhimurium and S. Enteritidis were 50%, 33.33% and 16.66%, respectively. Also, showed 100% of S. Typhimurium in both livers and minced meat. The AST in human isolates showed resistance to Ampicillin, Cefotaxime, Ceftazidime, Cefepime, Amikacin, Gentamicin, Ciprofloxacin, Norfloxacin, and Ceftriaxone, while no resistance to Amoxicillin, Pipracillin, Ertapenem, Imipenem, Meropenem, Fosfomycin, Nitrofurantoin, Trimethoprim, Azithromycin, and Tetracycline. In chicken products isolates were resistance with different percentages to Amikacin, Gentamicin, Tetracycline, Ciprofloxacin, Norfloxacin, Nitrofurantoin, Ampicillin, Cefotaxime, Ceftazidime, Cefepime, and Trimethoprim; while no resistance to Amoxicillin, Pipracillin, Ertapenem, Imipenem, Meropenem, Fosfomycin, Azithromycin and Ceftriaxone. Sequencing by using rRNA gene was done for four PCR products. Conclusion: This study showed the presence of genetic mutations for S. enterica which led to variations in the molecular characteristics, and antimicrobial drug resistance of S. enterica isolated from chicken products and human.

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration.

Minced meat is a valuable source of nutrients, but it is vulnerable to contamination by microorganisms commonly present in the environment. In addition, there is a risk of adulteration with cheaper meat sources, which can be harmful to consumers. It is crucial to identify meat adulteration with distinct microbiological analysis for legal, economic, religious, and public health purposes. A total of 100 minced meat samples were collected from several markets in Sharkia Governorate, Egypt. These samples were then subjected to bacteriological testing and an advanced multiplex PCR method. This method enables the detection of bovine, equine, porcine, and dog species in meat samples with just one step. The adulterated samples had a higher total bacterial count and pH values compared to pure bovine meat. These differences in bacterial count and pH values were statistically significant, with p-values of 0.843 (log10) and 0.233, respectively. The frequency of Escherichia coli occurrence was 13%, and the O111 serotype was predominant in the adulterated samples. Listeria monocytogenes and Staphylococcus aureus were isolated with prevalence rates of 3% and 29%, respectively. Besides, the SYBR-green multiplex real-time PCR assay used in this study detected adulteration with dog, equine, and porcine meats in the examined samples at rates of 9%, 5%, and 4%, respectively. This method provides a sensitive and specific approach to detect issues related to well-being and safety.

К ВОПРОСУ ПРИМЕНЕНИЯ ПИЩЕВЫХ ВОЛОКОН В ПРОИЗВОДСТВЕ МЯСНЫХ РУБЛЕНЫХ ПОЛУФАБРИКАТОВ

Quality of life, working capacity and longevity depend on what a person eats. Meat is one of essential nutritional factors, but it lacks carbohydrates. Dietary fiber is known to be important for prevention of human diseases (diabetes, obesity, cancer, cardiovascular diseases), therefore, recipes for meat products with food fiber are currently being developed. The article presents developed recipes for semi-finished minced meat products from poultry with the introduction of natural dietary wheat fibers «Unitsel VF 200» in place of the chicken fillet part. The study of functional, technological and organoleptic properties of experimental minced meat samples with additive «Unitsel VF 200» made it possible to obtain product containing complex of all nutritional elements, including proteins, fats, carbohydrates, dietary fiber, while meeting the necessary sensory characteristics. The optimal amount of added wheat fibers «Unitsel VF 200» to the composition of minced poultry meat was 24 % in dry form, 510 % in hydrated form.

Применение творожной СЫВОРОТКИ В ТЕХНОЛОГИИ КОЛБАСНО-СОСИСОЧНЫХ ИЗДЕЛИЙ с рыбной составляющей как направление рационального использования вторичного пищевого сырья

In this paper, we discuss the use of whey in food technologies. We discuss the feasibility of production by enterprises from Kamchatka Territory of products using whey as a secondary raw material and benign fish food waste from fillet. Introduction of these components into food compositions in the production of sausage products will provide the opportunity to implement the principle of rational use of biological resources and will expand the range of products that are in demand by consumers. Data from a study of methods for introducing curd whey into the minced meat mixture for the preparation of sausage products are presented. It has been shown that achieving high consumer (organoleptic) properties of the finished product is ensured by introducing whey into minced meat in the form of a suspension. The dependence of the taste and aroma properties and consistency of products on the amount of curd whey introduced into the recipe composition is characterized.

Antimicrobial Activity of Pomegranate Peel and Its Applications on Food Preservation

Multiplex PCR is efficient in detection of Staphyloccocuus aureus and L.monocytogenes in food and may be used in tracing of toxins to promote food hygiene. Implications of contamination of processed meat to highlight food hygiene . The purpose of this study was to use Multiplex PCR for detecting listeria monocytogenes and Staphylococcus aureus in processed beef meat product . S.aureus 23S rRNA gene and L. monocytogenes 16SrRNA gene were used to detect Staphyloccocuus aureus and L.monocytogenes respectively in the examined beef meat product . A total of 40 random processed meat samples (10 each of minced meat, beef burger, beef sausage and beef kofta) were collected from different super markets in the study area (El-Menofia governorate). The peels of pomegranate fruits are the major by-products produced during food processing of pomegranate enriched in antioxidants and broad-spectrum antimicrobial agents and can prevent food deterioration even. In this study , the effect of The pomegranate peel extract give high microbial activity against Staphyloccocuus aureus and L.monocytogenes in minced meat. The in vitro antibacterial and antioxidant properties of hydromethanolic extract of peel from Citrus sinensis (Sweet orange) was investigated. In this study the antibacterial activity of Citrus sinensis peel extract against Stapyloccous aureus and listeria monocytogenes was reported ,Citrus sinensis peels had shown mild activity against the two microbes .

РАЗРАБОТКА РЕЦЕПТУРЫ ПАШТЕТА НА ОСНОВЕ МЯСА СЕВЕРНОЙ КРЕВЕТКИ ДЛЯ ПИТАНИЯ ПОЖИЛЫХ ЛЮДЕЙ

A pate based on northern shrimp meat (Pandalus borealis), dairy cream and sunflower oil was developed to feed elderly. The optimal combination of main ingredients of the pate (% by weight of raw materials) was established: minced northern shrimp meat – 50.59%, milk cream – 30.59%, sunflower oil – 17.92%, based on a study of organoleptic, structural-mechanical and optical parameters of the final product. The pate based on northern shrimp meat contains 12.65% protein and 24.38% fat, which are important for a balanced diet for older people. The use of gentle heat treatment – pasteurization – contributed to the preservation of nutrients, improving the organoleptic and structural-mechanical properties of the product. The results of the study of microbiological and biochemical parameters during storage indicated stable storage of the product for 108 days at temperatures from 0 to 6°C.