Meat Inspection Research Articles

Core genome multilocus sequence typing (cgMLST) confirms systemic spread of avian pathogenic Escherichia coli (APEC) in broilers with cellulitis

Broiler cellulitis has emerged as an important cause of economic losses for farmers and slaughter plants from carcass condemnation at processing. Avian pathogenic Escherichia coli (APEC) has been identified as the main causative agent. The aim was to characterize E. coli isolated from cellulitis and organs in broilers at slaughter by whole genome sequencing analysis to study if systemic spread could be confirmed. Isolates were collected post-mortem from 101 carcasses condemned due to dermatitis/cellulitis from five commercial farms and six flocks. Forty-six isolates were characterised to determine serotypes, sequence types and virulence-associated genes. Analysis by cgMLST was performed to study the genetic similarity between isolates from the same broiler, among birds from the same flock and between flocks. Escherichia coli was isolated from 90% of birds from subcutaneous samples. In 20 broilers, E. coli was isolated from organs in pure culture or mixed with sparse growth of other bacteria. In eight of these, there were post-mortem findings suggestive of systemic bacterial spread. The majority of the isolates from the same bird and flock belonged to the same serotype and sequence type and were genetically indistinguishable, but differed when compared between flocks. Common APEC virulence genes, i.e. chuA, fyuA, hlyF, iroN, irp2, iss, ompT, sitA, TerC, TraT, were present in > 87% of the isolates. We conclude that evidence of systemic spread of E. coli from cellulitis was present in some birds at time of slaughter but cannot be reliably detected at meat inspection.

Presence of Foodborne Bacteria in Wild Boar and Wild Boar Meat-A Literature Survey for the Period 2012-2022.

The wild boar is an abundant game species with high reproduction rates. The management of the wild boar population by hunting contributes to the meat supply and can help to avoid a spillover of transmissible animal diseases to domestic pigs, thus compromising food security. By the same token, wild boar can carry foodborne zoonotic pathogens, impacting food safety. We reviewed literature from 2012-2022 on biological hazards, which are considered in European Union legislation and in international standards on animal health. We identified 15 viral, 10 bacterial, and 5 parasitic agents and selected those nine bacteria that are zoonotic and can be transmitted to humans via food. The prevalence of Campylobacter, Listeria monocytogenes, Salmonella, Shiga toxin-producing E. coli, and Yersinia enterocolitica on muscle surfaces or in muscle tissues of wild boar varied from 0 to ca. 70%. One experimental study reported the transmission and survival of Mycobacterium on wild boar meat. Brucella, Coxiella burnetii, Listeria monocytogenes, and Mycobacteria have been isolated from the liver and spleen. For Brucella, studies stressed the occupational exposure risk, but no indication of meat-borne transmission was evident. Furthermore, the transmission of C. burnetii is most likely via vectors (i.e., ticks). In the absence of more detailed data for the European Union, it is advisable to focus on the efficacy of current game meat inspection and food safety management systems.

MICROBIOLOGICAL ASSESSMENT OF BROILER CHICKEN MEAT FROM DIFFERENT SLAUGHTERHOUSES OF POKHARA VALLEY

Chicken meat is a nutritious food consuming all over the world having low cost and fat content which is highly perishable with short shelf life even when refrigerated in low temperature. Microbiological assessment was done by taking samples from different slaughtered houses of different wards of Pokhara and was evaluated in the lab. To assess the hygiene quality of chicken meat slaughtered in the slaughtered house of Pokhara Valley, six chicken meat samples were taken in the present work. Different microbiological parameters such as total coliforms, fecal coliforms, total plate count, Salmonella, Shigella, and Staphylococcus aureus were examined. A survey with the help of a questionnaire was also done to assess the sanitary condition of slaughtered houses and butchers. The average value for the total plate count of the meat sample was found to be 6.3406×106 cfu/g, only one sample was found to be Salmonella positive whereas all the samples were found to be Shigella negative. Only two samples were found to be Staphylococcus aureus positive. Likewise, two samples were found to contain total coliforms and there were no any formation of fecal coliform. Based on the results obtained, the risk of contamination depends on how the meat is processed, handled and packed. From the survey, it was clear that in some slaughtered houses, chicken meat is slaughtered unhygienically and unscientifically in an unmanaged way. There is a need for strict compulsion of the meat inspection act and education about sanitation for all the employees working in it.

Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi.

The lack of quick, accurate, and low-cost detection methods has hindered the active control strategies for bovine tuberculosis (bTB) in resource-limited countries with a high burden of disease. We developed a dry loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Mycobacterium bovis, the principal causative agent of bTB, and evaluated the efficacy of the assay using suspected bTB samples collected during routine meat inspection at major regional abattoirs in Malawi. Template genomic DNA was extracted directly from the granulomatous bTB-like lesion (crude extracted DNA), as well as growth from the incubated mycobacterial growth indicator tubes (MGIT). Field results were visualized by the naked eye within 40 min following a color change of the amplified products. The sensitivity and specificity of the dry LAMP assay while using 152 DNA samples extracted from MGIT with confirmed M. bovis results were 98% and 88%, respectively. When 43 randomly selected crude DNA samples from lesions were used, the sensitivity and specificity of the dry LAMP assay were 100% and 75%, respectively. Our LAMP assay offers the potential to meet the demands for a low-cost and rapid field detection tool for bTB in resource-limited countries in which bTB is endemic.

Evaluating remote post-mortem veterinary meat inspections on pig carcasses using pre-recorded video material

BackgroundOfficial meat inspections at small-scale slaughterhouses and game-handling establishments in geographically remote areas place a heavy burden on the meat-producing food business operators. By performing meat inspections remotely using live-streamed video, instead of on-site, the official control could meet the goals of sustainability, resilience and logistics. We investigated the agreement between the two approaches at pig slaughter. Two official veterinarians (OVs) inspected 400 pig carcasses at a Swedish slaughterhouse, with each pig being inspected on site by one OV and remotely by the other. After a period of 3 to 6 months, video recordings of the remote inspections were assessed again by the same OVs, thus enabling direct comparisons of previous on-site inspections and renewed video-based inspections within the same OV.ResultsAgreement across 22 finding codes was generally very high for both OVs. In all but one case (whether to fully condemn a carcass), for both OVs, Prevalence-Adjusted Bias-Adjusted kappa was well above 0.8, indicating ‘almost perfect agreement’.ConclusionsThis study supports earlier findings that reliable post-mortem inspections can be performed using video, and indicates higher agreement between remote and on-site inspections if the same OV performs both.

Using a value chain framework for food safety assessment of edible offals in Nairobi, Kenya

Edible offals comprise those parts of the animal considered fit for human consumption apart from the skeletal muscle, fat and attached skin. Edible offals form an important source of affordable animal protein and a delicacy for the larger Kenyan market. The offals are mainly traded in the informal markets which supplies at least 80% of the domestic market and where hygiene levels are low. The Meat Control Act Chapter 356, Laws of Kenya (Revised 2012) requires that offals be obtained from an officially declared food animal that has been slaughtered in a licensed slaughterhouse and declared fit for human consumption. According to the Meat Control Act, slaughterhouses in Kenya are classified as either export or local. The latter is subdivided into categories A, B, and C depending on their daily capacity, land size and the area they are authorized to supply meat and meat products. Each export slaughterhouse, category A and B slaughterhouses require a minimum of three meat inspectors to carry out inspection of carcasses and offals. The Directorate of Veterinary Services is the main regulator in Kenya on matters concerning safety of meat at the slaughterhouses. The Director of Veterinary Services appoints meat inspectors and veterinary officers who are responsible for ascertaining the safety of meat and meat products from the slaughterhouses. The meat inspectors also verify that slaughter facilities, staff, processes and equipment conform to food safety requirements. Offals, like carcass meat, should be handled under utmost hygiene conditions to minimize contamination or excessive growth of microorganisms. The current practices in slaughterhouses is that each set of edible offals is packaged separately in either polythene bags, plastic containers, meat transport containers or carriers and dispatched to the market. In some instances, stomachs and intestines are packaged for dispatch together with the lungs and hearts for transportation to distances over 50 km in either refrigerated or non-refrigerated containers or carriers. Some of these practices could compromise the safety of the offals. In this paper, we review the challenges faced in maintaining safety of edible offals especially at the slaughterhouses, distributors and retailers level from regulators' point of view.

Financial Losses Arising from Cattle Organ and Carcass Condemnation at Lokoloko Abattoir in Wau, South Sudan.

Slaughterhouses in South Sudan mirror the economic losses resulting from cattle organs and carcass condemnation due to zoonotic and epizootic diseases of livestock, such as tuberculosis, cysticercosis, and hydatidosis in cattle. However, due to the war, slaughterhouse record keeping has been inconsistent in South Sudan, and thus the estimation of diseases in cattle and their impact may be underestimated. Therefore, this study was conducted to estimate the major causes of carcasses and organ condemnation of cattle slaughtered at Lokoloko abattoir and the resulting financial losses. A cross-sectional active abattoir survey involving antemortem and postmortem examinations was conducted on 310 cattle between January 2021 and March 2021. Furthermore, five-year (September 2015-September 2020) retrospective data on meat inspection records were also collected and analyzed. During the antemortem inspection of the active abattoir survey, 103 (33.2%) cattle had signs of disease. These signs included herniam 17 (5.5%), local swelling 16 (5.2%), lameness 15 (4.8%), emaciation 13 (4.2%), blindness 12 (3.9%), depression 11 (3.5%), pale mucus membrane 7 (2.3%), nasal discharge 5 (1.6%), lacrimation 4 (1.3%), and salivation 03 (0.97%). Postmortem inspection revealed gross pathological findings on 180 (58.6%) carcasses, out of which 47 (26.1%) livers and 31 (17.2%) hearts were condemned due to various causes. The active abattoir survey and the retrospective data revealed that tuberculosis, fascioliasis, hydatidosis, and heart cysticercosis were the leading causes of condemnation of carcasses and organs. In the active abattoir survey, a total of 19,592,508 South Sudanese Pounds, equivalent to US$29,686 was lost from organ condemnation, while in the retrospective data; the overall direct financial loss during the five years was estimated to be 299,225,807 South Sudanese Pounds equivalent to US$453,372. This study revealed that bacterial and parasitic diseases were the common causes of carcass and organ condemnations and caused significant financial losses at Lokoloko abattoir in Wau, South Sudan. Therefore, there is a need for training farmers on cattle disease management, heightened meat inspections, and proper disposal of condemned meat.

Ante-mortem and Post-mortem Inspection and Relationship between Findings in a North Albanian Pig Slaughterhouse.

In June 2014, Albania was granted EU candidate status, thus starting a process of compliance with the membership criteria. In this context, a modern meat inspection approach in line with the European legislation was applied to a pig slaughterhouse in northern Albania in order to investigate the ante-mortem (AM) and post-mortem (PM) conditions and the relationship between these findings. For this purpose, 3930 pigs divided into 35 batches were evaluated over a 3-month period. The most frequent AM conditions recorded were tail lesions and dyspnea (9.1%), followed by skin (8.9%) and ear lesions (8.5%), while in the PM inspections, pleuritis was the most frequently observed condition (10.2%), followed by pneumonia (8.5%), liver alterations (5.7%), milk spot liver (3.8%), and pericarditis (3.3%). With the exception of liver alterations, the other PM lesions mentioned were positively associated with lesions on the ears (OR = 1.036; p < 0.001) and skin (OR = 1.026; p = 0.011) and dyspnea (OR = 1.021; p = 0.005), confirming the link between these variables and the health and welfare conditions of pigs on farms. Overall, the evidence that emerged from this Albanian slaughterhouse can be considered in line with other European contexts, especially in light of the considerable variability in the data present in the literature.

Cystic echinococcosis in donkeys in eastern Africa.

Cystic echinococcosis (CE) is endemic in humans and domestic animals in eastern Africa. All the species of the Echinococcus granulosus sensu lato complex have been reported in this region except for E. equinus, possibly due to the small number of studies involving equids. This study reports the frequency of different Echinococcus species in donkeys from eastern Africa. A total of 5961 donkeys were examined during meat inspection in 3 slaughterhouses in Kenya. Identification of Echinococcus spp. was achieved through polymerase chain reaction-restriction fragment-length polymorphism and sequencing of the mitochondrial nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 1 gene. The prevalence of CE was 5.7% (337/5961). The 263 genotyped cysts belonged to E. equinus (n = 163), E. granulosus sensu stricto (n = 70), E. canadensis (G6/7) (n = 26) and E. ortleppi (n = 4). One donkey harboured a metacestode of Spirometra theileri. All E. equinus cases, except 2, originated from southern Ethiopia, whereas the other species were more evenly distributed across the study area. Most of the cysts belonging to E. equinus were fertile (111/163), while those of the other species were non-fertile. This is the first report of Echinococcus spp. in donkeys from sub-Saharan Africa and the first confirmation of E. equinus in East Africa. The frequent fertility of E. equinus cysts in donkeys affirms their suitability as intermediate hosts of this species, while low frequency and cyst fertility suggest a marginal role of donkeys in the transmission of E. granulosus s. s., E. canadensis (G6/7) and E. ortleppi.

The Importance of the Slaughterhouse in Surveilling Animal and Public Health: A Systematic Review.

From the point of public health, the objective of the slaughterhouse is to guarantee the safety of meat in which meat inspection represent an essential tool to control animal diseases and guarantee the public health. The slaughterhouse can be used as surveillance center for livestock diseases. However, other aspects related with animal and human health, such as epidemiology and disease control in primary production, control of animal welfare on the farm, surveillance of zoonotic agents responsible for food poisoning, as well as surveillance and control of antimicrobial resistance, can be monitored. These controls should not be seen as a last defensive barrier but rather as a complement to the controls carried out on the farm. Regarding the control of diseases in livestock, scientific research is scarce and outdated, not taking advantage of the potential for disease control. Animal welfare in primary production and during transport can be monitored throughout ante-mortem and post-mortem inspection at the slaughterhouse, providing valuable individual data on animal welfare. Surveillance and research regarding antimicrobial resistance (AMR) at slaughterhouses is scarce, mainly in cattle, sheep, and goats. However, most of the zoonotic pathogens are sensitive to the antibiotics studied. Moreover, the prevalence at the slaughterhouse of zoonotic and foodborne agents seems to be low, but a lack of harmonization in terms of control and communication may lead to underestimate its real prevalence.

The Value of Current Ante Mortem Meat Inspection and Food Chain Information of Dairy Cows in Relation to Post Mortem Findings and the Protection of Public Health: A Case for a More Risk-Based Meat Inspection.

In this study, the contribution of the ante mortem (AM) inspection and the food chain information (FCI) to ensuring meat safety and public health was investigated, by evaluating the slaughterhouse findings of 223,600 slaughtered dairy cows in the Netherlands. The outcome of this study was that the ante mortem (AM) and post mortem (PM) inspections have a substantial overlap, and that with regard to food safety and public health in over 99% of cases the PM could even be omitted on the basis of the AM. In this study, the data provided by the dairy farmers on the current FCI forms contributed little to nothing with regard to the outcomes of AM and PM inspection. It is concluded that current meat inspection procedures need an update and a more risk-based approach needs to be adopted. Regarding this, the AM inspection of dairy cattle should remain, because it plays an important role in ensuring food safety (e.g., by preventing contamination of the slaughter line by excessively dirty animals, or animals with abscesses), monitoring animal welfare and in detecting some important notifiable diseases. The PM inspection, however, could in many cases be omitted, provided there is a strict AM inspection complemented by a vastly improved (automated) way of obtaining reliable FCI.

Peter Joseph Zanetti.

Initially a small animal vet, he worked for the PDSA before becoming a meat inspector.

Exploring potential risk pathways with high risk groups for urban Rift Valley fever virus introduction, transmission, and persistence in two urban centers of Kenya.

Rift Valley fever virus (RVFV) is a zoonotic arbovirus that has profound impact on domestic ruminants and can also be transmitted to humans via infected animal secretions. Urban areas in endemic regions across Africa have susceptible animal and human hosts, dense vector distributions, and source livestock (often from high risk locations to meet the demand for animal protein). Yet, there has never been a documented urban outbreak of RVF. To understand the likely risk of RVFV introduction to urban communities from their perspective and guide future initiatives, we conducted focus group discussions with slaughterhouse workers, slaughterhouse animal product traders, and livestock owners in Kisumu City and Ukunda Town in Kenya. For added perspective and data triangulation, in-depth interviews were conducted one-on-one with meat inspector veterinarians from selected slaughterhouses. A theoretical framework relevant to introduction, transmission, and potential persistence of RVF in urban areas is presented here. Urban livestock were primarily mentioned as business opportunities, but also had personal sentiment. In addition to slaughtering risks, perceived risk factors included consumption of fresh milk. High risk groups' knowledge and experience with RVFV and other zoonotic diseases impacted their consideration of personal risk, with consensus towards lower risk in the urban setting compared to rural areas as determination of health risk was said to primarily rely on hygiene practices rather than the slaughtering process. Groups relied heavily on veterinarians to confirm animal health and meat safety, yet veterinarians reported difficulty in accessing RVFV diagnostics. We also identified vulnerable public health regulations including corruption in meat certification outside of the slaughterhouse system, and blood collected during slaughter being used for food and medicine, which could provide a means for direct RVFV community transmission. These factors, when compounded by diverse urban vector breeding habitats and dense human and animal populations, could create suitable conditions for RVFV to arrive an urban center via a viremic imported animal, transmit to locally owned animals and humans, and potentially adapt to secondary vectors and persist in the urban setting. This explorative qualitative study proposes risk pathways and provides initial insight towards determining how urban areas could adapt control measures and plan future initiatives to better understand urban RVF potential.

Serodiagnosis of Porcine Cysticercosis and Risk Assessments in Pigs Slaughtered in Ywar Thar Gyi Slaughter within Yangon Region, Myanmar

Since 2015, porcine cysticercosis has been under-reported in Myanmar, even though the country was known to be endemic. This study aimed to figure out the seroprevalence of porcine cysticercosis and risk factors relating to pigs' infection by administering questionnaires to the farm owners. We bled a total of 330 pigs to collect the sera sample before slaughter at the Ywar Thar Gyi slaughter, the major abattoir in Yangon, Myanmar. The monoclonal antibody-based B158/B60 Ag-ELISA technique was used since it has shown high diagnostic values. The presence of Taenia spp. cyst was also assessed by meat inspection of six different muscles. We also investigated the distribution of cysts in slaughtered pigs. Of 330 blood samples, 14.24% of pigs were tested as seropositive. However, 12.12% of pigs were examined for the presence of cysticerci by the meat inspection method. The comparison between seroprevalence and prevalence by meat inspection was made using a 2x2 matrix table. Regarding cyst distribution, 95.0% (38/40) observed cysticerci on the ventral surface of the tongue muscle, and we found the remaining two positive samples inside the heart muscle. Results have shown that husbandry practice (OR=5.1; 95% CI=2.9-8.5) and deworming practice (OR=4.1; 95%CI=2.5-7.5) had a significant association (P&lt;0.05) with the presence of infection in slaughter pigs. This study indicates the presence of cysticercosis in slaughtered pigs, and the results could assist the need for further studies for the intervention of effective control strategies.

Association of Live Vaccine with Swine Erysipelas Found in Meat Inspection

Association of Live Vaccine with Swine Erysipelas Found in Meat Inspection

Seroprevalence of Taenia saginata Cysts in Cattle, Human Hospital Cases, and Risk Factors for Human Taeniasis in Kajiado County, Kenya.

Taenia saginata is a zoonotic tapeworm that causes diseases in cattle and humans. A cross-sectional survey was conducted between June and July 2021 in Kajiado County, Kenya, to estimate the seroprevalence of Taenia saginata cysts in cattle presented for slaughter in Kitengela, Kiserian, and Keekonyokie slaughterhouses; determine the annual hospital reported cases of T. saginata in humans that visited all level 4 hospitals in Kajiado County between 2015 and 2021; and assess the risk factors for Taenia saginata in humans. Analyzed data in this study revealed that the seroprevalence of Taenia saginata cysts in cattle in the selected slaughterhouses was 2.67% (4/150). The meat inspectors recorded no cysts during the study period, confirming that antibody ELISA is three times as sensitive as meat inspection. Data from hospital records showed that a total of 1,487,687 patients visited different facilities in Kajiado County between 2015 and 2021. During the same period, 29 patients were diagnosed with human taeniasis. From the risk factors assessed, uninspected home slaughter (75%), consumption of raw/improperly cooked beef (100%), and open defecation by herders (11%) still pose a risk to humans getting infected by Taenia saginata, while the presence and use of latrines (89%) and availability, accessibility, and use of taenicides (89%) seem to have significantly contributed to the reduction of Taenia saginata prevalence in this region. It is recommended that control of the infection should be centered towards continuous focused education coupled with regular deworming of the herders and school going children. This will gradually result in behavior and culture change that will ultimately reduce the prevalence and lead to the elimination of the disease.

A Marked Decline in Taenia solium Taeniasis and Cysticercosis Infections in China: Possible Reasons from the Ecological Determinants Perspective

Taenia solium taeniasis/cysticercosis is an important global food-borne infectious disease transmitted between humans and pigs. According to both national surveys and field investigations, the prevalence of the disease in China has significantly decreased in recent decades. The primary disease control measures are health education and promotion, meat inspection, and chemotherapy. Other factors that influence or fundamentally affect human and pig T. solium taeniasis/cysticercosis have been identified, such as pig farming patterns shift and a revolution in sanitary toilets, which block T. solium transmission routes. Pig farming practices have shifted from backyard to large-scale intensive farming, thus decreasing pig contact with, and consumption of, human excreta. The increased in the use of sanitary toilets has facilitated hygiene by preventing of human excreta from contacting humans, or polluting the environment, feed, or water. The occurrence of human T. solium infections has markedly decreased as a result of these social changes. The objective of this review is to describe the ecological determinants of the cysticercosis decline in China.

A Trichinella britovi outbreak in the Northern Alps of France: investigation by a local survey network.

We describe a small family outbreak of trichinellosis caused by the consumption of raw ham from a wild boar (Sus scrofa) hunted in the northern Alps of France in February 2022. Out of the six people, aged 3-69 years, who consumed the meat, three were confirmed cases, and three were suspected cases. Eosinophilia detected in four people was the hallmark that drove the diagnosis. Three patients presented with myalgia, two with intense and prolonged chest pain, and one with elevated troponin. One patient presented with dermographism during treatment. Anti-Trichinella IgG were detected in three symptomatic individuals after about ten weeks. One patient had negative serology and no symptoms, but was on long-term corticosteroid therapy. Trichinella britovi larvae (8.3 larvae/g) were detected in the wild boar meat remnants. Trichinellosis is rare in France, but this family outbreak is reminiscent of the circulation of this pathogen in wild animals, highlighting the need to inform hunters about the risk of infection linked to the consumption of raw meat of game animals, and about the need for veterinary inspection of game meat. The consumption of raw meat outside controlled circuits is a practice not devoid of risks, which justifies raising the awareness of hunters, doctors, and medical biologists.

Investigation of the Occurrence of Lungworm Pneumonia in Sheep Based on Histopathological Study in Tehran Province

Lung parasites are one of the main causes of respiratory diseases in sheep in most parts of the world. The survival and development of lung parasites in sheep occurs more in tropical and subtropical regions of the world and this leads to the removal of this organ, which includes many economic losses. In this study, the lungs of 4500 sheep from various farms in an industrial slaughterhouse in Tehran province were examined. After slaughter, common methods of meat inspection were used to identify pathological lesions in carcasses using macroscopic examinations. As a result, macroscopic pneumonia was identified in the lungs of 420 sheep (9.33%). Then, based on specific features such as the presence of gray-white nodules, brown spots, and the presence of adult worms, 65 lungs suspected of lungworm pneumonia were identified and tissue samples were taken.

PRECONDITIONS FOR THE CREATION OF A MEAT FRESHNESS CONTROL AND IDENTIFICATION SYSTEM

The relevance of creating a comprehensive system for meat control and identification to determine its freshness level has been demonstrated in the study. The drawbacks of traditional organoleptic and laboratory methods commonly used for meat inspection were analyzed. The authors presented the advantages and challenges of employing an electronic nose. A design for a meat control and identification system was proposed, which includes an Arduino Uno microcontroller, Raspberry Pi, USB to TTL adapter, gas sensors, color sensor, thermal camera, and image sensor. The proposed implementation of the electronic nose system on a single-board computer demonstrates its success in controlling and identifying meat freshness. A matrix of semiconductor gas sensors, TGS2602, MQ137, and MQ138, is formed as olfactory sensors, and TCS3200 is used as an RGB vision sensor, enabling the identification of the smell and color of different degrees of meat freshness. To obtain clear output differences from the gas sensors that react to the freshness level of meat, the baseline method is proposed for use. Therefore, a system enhanced with neural network capabilities will replace traditional devices for identifying meat freshness.